当前位置： SCI文献检索 > TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE期刊下所有文献 > Semi-quantitative detection of Mycobacterium leprae antigens in skin scrapings: suitability as a laboratory aid for field diagnosis of leprosy.

Semi-quantitative detection of Mycobacterium leprae antigens in skin scrapings: suitability as a laboratory aid for field diagnosis of leprosy.

Abstract：

:We describe here a method, potentially suitable for field applications, for semi-quantitative detection of Mycobacterium leprae antigens in skin scrapings, which are taken normally for smear microscopy. Thirty acid-fast bacilli-negative paucibacillary (PB) leprosy patients comprised the main study group; eight acid-fast bacilli-positive multibacillary (MB) patients and five healthy laboratory workers served as controls. Samples in saline were spotted on nitrocellulose paper and probed with mycobacterium-specific polyclonal or M. leprae-specific mAbs against 12, 35 and 65kDa protein antigens, using a dot-ELISA format. Spot densities were read through a densitometer and also graded visually. The polyclonal antibody produced the best sensitivity, resulting in densitometric detection of mycobacterial antigen in 100% MB, 76% multiple-lesion PB and 62% single-lesion PB patients. None of the healthy volunteers showed antigen positivity. A correlation was noted between the densitometric and visual estimates of the antigen. Determination of antigen in the lesion and an apparently uninvolved area of skin in a subset of PB patients provided clues to the state of the underlying infection. Serological positivity of PB patients for M. leprae-specific antibodies against the 35kDa and phenolic glycolipid-I antigens was too low (<20%) for any diagnostic significance.

journal_name

Trans R Soc Trop Med Hyg

journal_title

Transactions of the Royal Society of Tropical Medicine and Hygiene

authors

Chaturvedi V,Girdhar BK,Sengupta U,Sinha S

doi

10.1016/j.trstmh.2007.02.026

subject

Has Abstract

pub_date

2007-07-01 00:00:00

pages

699-706

issue

7

eissn

0035-9203

issn

1878-3503

pii

S0035-9203(07)00067-3

journal_volume

101

pub_type

杂志文章

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