Abstract:
:Melatonin possesses a number of important biologic activities including oncostatic, anti-oxidant, and immunostimulatory actions. This study was designed to assess the effects of melatonin on inflammation-related gene expression in lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMCs), using CombiMatrix 2K Human Inflammation chip. After pretreatment with melatonin (100 microm) for 4 hr, cells were incubated with LPS (1 microg/mL) for 24 hr. We compared gene expression profiles between LPS-treated, melatonin-treated, LSP/melatonin-treated, and control groups. LPS induced the upregulation of 95 genes, compared with controls. Melatonin pretreatment in LPS-stimulated PBMCs suppressed the expression of 23 genes more than twofold. Interestingly, melatonin showed a suppressive effect on the expression of CC chemokine subfamily genes, including CCL2/MCP1, CCL3/MIP1 alpha, CCL4/MIP1 beta, CCL5/RANTES, CCL8/MCP2, CCL20/MDC, and CCL22/MIP3 alpha, in LPS-stimulated PBMCs. This result was confirmed by reverse transcriptase polymerase chain reaction. Among the CC chemokine subfamily genes, particularly, the expression of CCL2 and CCL5 was markedly downregulated by melatonin in LPS-stimulated PBMCs. The secretion levels of CCL2 and CCL5 were measured using enzyme-linked immunosorbent assay. Stimulation of PBMCs by LPS induced the secretion of CCL2 (2334.3 +/- 161.4 pg/mL, mean +/- S.E.M.), whereas melatonin pretreatment (153.0 +/- 3.8 pg/mL) inhibited the LPS-induced secretion of CCL2. Melatonin pretreatment (2696.2 +/- 385.3 pg/mL) also inhibited the LPS-induced secretion of CCL5 (4679.6 +/- 107.5 pg/mL). Taken together, these results suggest that melatonin may have a suppressive effect on LPS-induced expression of CC chemokine genes, especially CCL2 and CCL5, which may explain its beneficial effects in the treatment of various inflammatory conditions.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
Park HJ,Kim HJ,Ra J,Hong SJ,Baik HH,Park HK,Yim SV,Nah SS,Cho JJ,Chung JHdoi
10.1111/j.1600-079X.2007.00452.xsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
121-9issue
2eissn
0742-3098issn
1600-079Xpii
JPI452journal_volume
43pub_type
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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pub_type: 杂志文章
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1996.tb00289.x
更新日期:1996-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-079x.1993.tb00498.x
更新日期:1993-05-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1998.tb00526.x
更新日期:1998-04-01 00:00:00
abstract::Melatonin has been shown to improve in vitro fertilization and offspring survival after bacterial infection, but its role in regulating maternal-fetal communication during early pregnancy has not been investigated. Results of this study demonstrated expression of abundant melatonin receptors in conceptus and endometri...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12670
更新日期:2020-09-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00550.x
更新日期:2008-05-01 00:00:00
abstract::Resistance inducers are a class of agrochemicals, including benzothiadiazole and chitosan, which activate the plant own defence mechanisms. In this work, open-field treatments with plant activators were performed on two red grape (Vitis vinifera L.) varieties cultivated in different localities, Groppello (Brescia, Lom...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2011.00887.x
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abstract::Earlier studies showed that melatonin reduced the growth of 17-beta-estradiol (E(2))-induced rat pituitary prolactin-secreting tumor (prolactinoma) in vivo. The mechanisms of melatonin's inhibitory action on the prolactin-secreting tumor were further explored by investigating the in vitro effects of melatonin on the g...
journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2009.00719.x
更新日期:2009-11-01 00:00:00
abstract::Maternal hyperthermia induces early embryonic death via increased oxidative stress to the embryo. In this study, we examined whether melatonin administered to heat-stressed pregnant mice would reduce hyperthermia-induced embryonic death. Mice were heat stressed (12 hr at 35 degrees C, 60% relative humidity) on the day...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2005.00260.x
更新日期:2005-10-01 00:00:00
abstract::A review of the literature indicates that the absolute oral bioavailability of exogenous melatonin in humans or in preclinical animal models has not been adequately characterized; hence, this study was undertaken. Pharmacokinetics of melatonin was studied in rats, dogs, and monkeys following intravenous and oral admin...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1997.tb00302.x
更新日期:1997-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-079x.1993.tb00503.x
更新日期:1993-08-01 00:00:00
abstract::Melatonin is a powerful scavenger of oxygen free radicals. In humans, melatonin is rapidly transferred from the maternal to the fetal circulation. To investigate whether or not maternal melatonin administration can protect the fetal rat brain from radical-induced damage by increasing the activities of antioxidant enzy...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1034/j.1600-079x.2001.280204.x
更新日期:2000-03-01 00:00:00
abstract::Melatonin is an excellent free radical scavenger, reacting with tert-butoxyl and cumyloxyl radicals with rate constants of 3.4 x 10(7) and 6.7 x 10(7) M-1s-1, respectively. Reaction with benzophenone triplet occurs with a near-diffusion-controlled rate constant of 7.6 x 10(9) M-1s-1 in acetonitrile and probably involv...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1995.tb00188.x
更新日期:1995-11-01 00:00:00
abstract::Transgenic rice plants overexpressing a sheep serotonin N-acetyltransferase led to an enhanced production of melatonin with various physiological effects, including seminal root elongation and resistance against cold and oxidative stress, which raises the possibility that melatonin may alter gene expression profiles i...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12077
更新日期:2013-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12705
更新日期:2020-11-19 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-079X.2009.00728.x
更新日期:2010-01-01 00:00:00
abstract::The effect of melatonin was evaluated on three phosphatidylcholine-based membrane models. Changes in liposome dynamics were monitored by fluorescence, following the response of the probe merocyanine-540, as well as by differential scanning calorimetry (DSC). Langmuir monolayers were investigated using molecular area m...
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pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00