当前位置： SCI文献检索 > JOURNAL OF REPRODUCTIVE IMMUNOLOGY期刊下所有文献 > Phytohaemagglutinin as a molecular probe to study the membrane constituents of human X- and Y-bearing spermatozoa.

Phytohaemagglutinin as a molecular probe to study the membrane constituents of human X- and Y-bearing spermatozoa.

Abstract：

:Phytohaemagglutinin (PHA) was employed to study the constituents of the cell membrane in X- and Y-bearing human spermatozoa. The presence of certain receptors for PHA was revealed in the equatorial region of about 50% of the spermatozoa. To determine the association of the equatorial staining by PHA with one of the subpopulations of spermatozoa a double staining technique was developed. In this method, the membrane was first stained with PHA employing the indirect immunofluorescence technique and then followed by the conventional quinacrine staining method to demonstrate the F-body. It was shown that spermatozoa which had receptors in the equatorial region for PHA were the ones which exhibited in the F-body. Staining of the membrane with PHA is a better method for the identification of human Y spermatozoa since it does not have the drawbacks of quinacrine staining.

journal_name

J Reprod Immunol

journal_title

Journal of reproductive immunology

authors

Hegde UC,Shastry PR,Rao SS

doi

10.1016/0165-0378(81)90005-x

subject

Has Abstract

pub_date

1981-03-01 00:00:00

pages

351-7

issue

6

eissn

0165-0378

issn

1872-7603

pii

0165-0378(81)90005-X

journal_volume

2

pub_type

杂志文章

相关文献

JOURNAL OF REPRODUCTIVE IMMUNOLOGY文献大全
  • Pravastatin prevents miscarriages in antiphospholipid antibody-treated mice.

    abstract：:Miscarriages in patients with antiphospholipid (aPL) antibodies have been attributed to thrombosis of placental vessels. However, we have shown that inflammation plays a crucial role in fetal injury. We identified tissue factor (TF), the major cellular activator of the coagulation cascade, as a key mediator in inflamm...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2009.03.003

    authors： Girardi G

    更新日期：2009-11-01 00:00:00

  • Innate and adaptive immunity against herpes simplex virus type 2 in the genital mucosa.

    abstract：:Herpes simplex virus type 2 (HSV-2) is becoming increasingly prevalent worldwide, despite the widespread use of antiviral drugs. Its ability to evade the immune system and establish a latent infection has made it difficult to develop an effective vaccine. Our understanding of the immune response against HSV-2 remains ...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jri.2011.01.001

    authors： Chan T,Barra NG,Lee AJ,Ashkar AA

    更新日期：2011-03-01 00:00:00

  • Post-obstruction rat sperm autoantigens identified by two-dimensional gel electrophoresis and western blotting.

    abstract：:Although antisperm autoantibody responses to obstruction of the male reproductive system have been documented, information on the nature of the cognate sperm autoantigens has been limited. In the present study, the patterns of sperm autoantigens recognized by sera from rats after obstruction of the vas deferens or epi...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/s0165-0378(98)00090-4

    authors： Flickinger CJ,Bush LA,Williams MV,Naaby-Hansen S,Howards SS,Herr JC

    更新日期：1999-05-01 00:00:00

  • Indications of anti-HY immunity in recurrent placental abruption.

    abstract：PROBLEM:Placental abruption is a potential life-threatening condition for both the fetus and the mother, being significantly more common in pregnancies with male fetuses. The pathogenesis of placental abruption remains unknown. However, some recent reports point toward a maternal immune response against the fetus as a ...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2007.03.003

    authors： Nielsen HS,Mogensen M,Steffensen R,Kruse C,Christiansen OB

    更新日期：2007-08-01 00:00:00

  • Secondary recurrent miscarriage is associated with previous male birth.

    abstract：:Secondary recurrent miscarriage (RM) is defined as three or more consecutive pregnancy losses after delivery of a viable infant. Previous reports suggest that a firstborn male child is associated with less favourable subsequent reproductive potential, possibly due to maternal immunisation against male-specific minor h...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2010.10.004

    authors： Ooi PV,Russell N,O'Donoghue K

    更新日期：2011-01-01 00:00:00

  • Tumor necrosis factor-alpha soluble receptor p55 (sTNFp55) and risk of preeclampsia in Peruvian women.

    abstract：:We conducted a large case-control study to assess the risk of preeclampsia with elevated sTNFp55 concentrations (markers of excessive TNF-alpha release) in Peruvian women. A total of 125 women with preeclampsia and 179 normotensive women were included in a study conducted during the period, June 1997 through January 1...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/s0165-0378(99)00064-9

    authors： Sanchez SE,Zhang C,Williams MA,Ware-Jauregui S,Larrabure G,Bazul V,Farrand A

    更新日期：2000-05-01 00:00:00

  • Uterine stromal cell suppression of pIgR production by uterine epithelial cells in vitro: a mechanism for regulation of pIgR production.

    abstract：:Previous studies have shown that the polymeric Ig receptor (pIgR) is produced by rat uterine epithelial cells both in vivo and vitro. The expression of the pIgR is regulated by sex hormones and/or cytokines at mucosal sites, however the mechanism of regulation in the uterus is not clear. In these studies, co-culture o...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/s0165-0378(97)00015-6

    authors： Richardson JM,Wira CR

    更新日期：1997-06-01 00:00:00

  • Risk factors for pregnancy failure in patients with antiphospholipid antibody positivity and prior pregnancy losses: A retrospective study.

    abstract：OBJECTIVES:To analyze the risk factors associated with pregnancy failure in patients with positive antiphospholipid antibodies (aPL) and prior pregnancy losses, with or without a diagnosis of antiphospholipid syndrome. METHODS:We retrospectively reviewed the medical records of all the pregnant patients with positive a...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2020.103171

    authors： Duan H,Li X,Liu C,Ge Y,Deng X

    更新日期：2020-09-01 00:00:00

  • Differential distribution of interleukin-1 alpha and interleukin-1 beta proteins in human placentas.

    abstract：:Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) were identified in samples of first trimester and term placentas by immunocytochemistry using two sets of polyclonal antibodies to recombinant IL-1. All samples contained the two species of IL-1, which predominated in different types of cells: IL-1 al...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(92)90047-8

    authors： Hu XL,Yang Y,Hunt JS

    更新日期：1992-10-01 00:00:00

  • Characterisation of the soluble fraction of human syncytiotrophoblast microvillous plasma membrane-associated proteins.

    abstract：:Syncytiotrophoblast microvillous plasma membrane (StMPM) preparations were obtained from human full-term placentae by previously published methods of cold saline extraction and phase centrifugation. Purity of these preparations was assessed by electron microscopy, enzyme analysis and hydroxyproline content. IgG, album...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(79)90013-5

    authors： Ogbimi AO,Johnson PM,Brown PJ,Fox H

    更新日期：1979-07-01 00:00:00

  • Intrauterine administration of autologous hCG- activated peripheral blood mononuclear cells improves pregnancy outcomes in patients with recurrent implantation failure; A double-blind, randomized control trial study.

    abstract：:We aimed to investigate the effect of intrauterine administration of autologous hCG-activated PBMCs in RIF women with low Th-17/Treg cell ratio. 248 women with a history of implantation failure volunteered to receive PBMC-therapy. After immunologic consultation and doing flow cytometry analysis, 100 women with at leas...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2020.103182

    authors： Pourmoghadam Z,Soltani-Zangbar MS,Sheikhansari G,Azizi R,Eghbal-Fard S,Mohammadi H,Siahmansouri H,Aghebati-Maleki L,Danaii S,Mehdizadeh A,Hojjat-Farsangi M,Motavalli R,Yousefi M

    更新日期：2020-11-01 00:00:00

  • Peptide presentation and NK inhibition by HLA-G.

    abstract：:The expression of the nonclassical MHC class Ib molecule HLA-G is nearly exclusively restricted to the feto-maternal interface during pregnancy. There it probably serves the same physiological functions already known for classical MHC class I molecules; these include peptide presentation, natural killer cell (NK) inhi...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/s0165-0378(99)00029-7

    authors： Münz C,Stevanović S,Rammensee HG

    更新日期：1999-07-01 00:00:00

  • Characterization of myeloid cell populations in human testes collected after sex reassignment surgery.

    abstract：:The testis has been described in animal models as a site of immune privilege, which protects spermatids against tissue damage during inflammation. Myeloid cells, including macrophages and dendritic cells (DC), are defined as key players in the testicular immune privilege in animal models. However, their distribution a...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2017.10.043

    authors： Ponte R,Dupuy FP,Brimo F,Mehraj V,Brassard P,Belanger M,Yurchenko E,Jenabian MA,Bernard NF,Routy JP,ORCHID study group.

    更新日期：2018-02-01 00:00:00

  • Indomethacin blocks the immunosuppressive activity of rat testicular macrophages cultured in vitro.

    abstract：:Macrophages are a prominent resident cell type in the interstitial tissue of the testis in several mammalian species. This presence in an immunologically-privileged site prompted an investigation of their ability to initiate and regulate lymphocyte proliferation in vitro. Isolated rat peripheral blood lymphocytes (PBL...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(95)91391-q

    authors： Kern S,Maddocks S

    更新日期：1995-03-01 00:00:00

  • Spliceosome protein EFTUD2 is upregulated in the trophoblast of spontaneous miscarriage and hydatidiform mole.

    abstract：BACKGROUND:Elongation factor Tu GTP binding domain containing 2 (EFTUD2) is an alternative splicing factor that modulates cell differentiation and activation processes. EFTUD2 is known to modulate immune responses and mutation of the EFTUD2-gene lead to fetal malformation. Little is known about its expression and role ...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2020.103149

    authors： Löb S,Vattai A,Kuhn C,Schmoeckel E,Mahner S,Wöckel A,Kolben T,Szekeres-Bartho J,Jeschke U,Vilsmaier T

    更新日期：2020-08-01 00:00:00

  • Prednisone improves pregnancy outcome in repeated implantation failure by enhance regulatory T cells bias.

    abstract：OBJECTIVE:Repeated implantation failure (RIF) has been shown related to maternal immune imbalance. Many studies suggested that prednisone promoted the Th17/Treg balance shift to the direction of immune tolerance. Our study aimed to evaluate the role of prednisone in Th17/Treg balance and pregnancy outcome in RIF patien...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2020.103245

    authors： Huang Q,Wu H,Li M,Yang Y,Fu X

    更新日期：2021-02-01 00:00:00

  • Peripheral blood T- and B-cell immunophenotypic abnormalities in selected women with unexplained recurrent miscarriage.

    abstract：:We aimed to investigate if women with recurrent miscarriage disclosed abnormalities in the maturation and activation status of peripheral blood lymphocyte subsets. In a case control study, 24 women with recurrent miscarriage, 37 women with children but no history of miscarriage and 39 women without previous pregnancie...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2015.11.003

    authors： Carbone J,Sarmiento E,Gallego A,Lanio N,Navarro J,García S,Fernandez-Cruz E

    更新日期：2016-02-01 00:00:00

  • Prostaglandin F2 alpha immunization of prepubertal beef heifers: effects of conjugate dose and timing of immunization relative to puberty on the onset of puberty and subsequent ovarian function.

    abstract：:Fifty-six prepubertal Hereford cross Friesian heifers were assigned to seven treatment groups: (1) primary (day 0) and booster (day 41) using 10 mg human serum albumin (HSA) (control); (2) primary and booster (day 41) immunizations using 3.3 mg prostaglandin F2 alpha PGF-HSA conjugate; (3) primary and booster (day 83)...

    journal_title：Journal of reproductive immunology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0165-0378(94)90006-x

    authors： Crowe MA,Enright WJ,Roche JF

    更新日期：1994-12-01 00:00:00

  • Molecular signature and functional analysis of uterine ILCs in mouse pregnancy.

    abstract：:In addition to natural killer cells, other innate lymphoid cells have recently been identified in the mouse and human uterus, but their roles in successful pregnancy remain poorly defined. In this study, we examined the dynamic changes of uterine innate lymphoid cells throughout pregnancy in mice. We found that the to...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2017.09.003

    authors： Li M,Gao Y,Yong L,Huang D,Shen J,Liu M,Ren C,Hou X

    更新日期：2017-09-01 00:00:00

  • The debate on the presence of HIV-1 in human gametes.

    abstract：:The debate about the presence of HIV-1 particles in human gametes and recent experimental results are reported in detail. Using immunocytochemistry, in situ hybridization at electron microscopy level, polymerase chain reaction and in vitro fertilization, it has been demonstrated that human spermatozoa can incorporate ...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章,评审

    doi：10.1016/s0165-0378(98)00048-5

    authors： Baccetti B,Benedetto A,Collodel G,di Caro A,Garbuglia AR,Piomboni P

    更新日期：1998-12-01 00:00:00

  • If we know so much about preeclampsia, why haven't we cured the disease?

    abstract：:Preeclampsia has been recognized for at least 100 years. In the last 20 years, the consideration of the disorder as more than simply hypertension in pregnancy has led to an explosion in knowledge about preeclampsia pathophysiology. It is now evident that for most cases of preeclampsia, the root cause is the placenta. ...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jri.2013.05.003

    authors： Roberts JM,Bell MJ

    更新日期：2013-09-01 00:00:00

  • Immunochemical identification of human trophoblast membrane antigens using monoclonal antibodies.

    abstract：:Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and ac...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(83)90244-9

    authors： Brown PJ,Molloy CM,Johnson PM

    更新日期：1983-11-01 00:00:00

  • Hormonal regulation of serum and endometrial IL-1 alpha, IL-1 beta and IL-1ra: IL-1 endometrial microenvironment of the human embryo at the apposition phase under physiological and supraphysiological steroid level conditions.

    abstract：:We have investigated serum and intracavitary levels of IL-1 alpha, IL-1 beta and IL-1ra from agonadal women undergoing mock cycles (n = 20) of oocyte donation as a clinical model of controlled hormonal stimulation. Further, we compared the intracavitary IL-1 alpha, IL-1 beta and IL-1ra levels in the microenvironment o...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(96)00982-5

    authors： Simón C,Mercader A,Frances A,Gimeno MJ,Polan ML,Remohí J,Pellicer A

    更新日期：1996-10-01 00:00:00

  • Suppression of fertility in male mice by immunization against LH receptor.

    abstract：:We have investigated the potential contraceptive effects of immunization against the luteinizing hormone (LH) receptor in male mice at the prepubertal stage. Two N-terminal fragments of the porcine LH receptor encoding amino acids 1-297 and 1-370 were produced in large quantities through the Baculovirus insect cell sy...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(93)90042-g

    authors： Remy JJ,Bozon V,Couture L,Goxe B,Salesse R,Garnier J

    更新日期：1993-09-01 00:00:00

  • Fc receptor blocking antibodies after active immunization for the treatment of recurrent spontaneous abortion.

    abstract：:In a prospective study 140 couples who had at least three spontaneous abortions (RSA) were studied for the presence of Fc receptor blocking antibodies detected by the erythrocyte antibody rosette inhibition (EAI) assay, for anti-paternal cytotoxic antibodies (APCA), and for mixed lymphocyte culture inhibiting (MLCI) a...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(91)90030-t

    authors： Kuhn U,Blasczyk R,Hojnacki B,Vögeler U,Luboldt W,Passarge E,Fischer WM,Grosse-Wilde H

    更新日期：1991-07-01 00:00:00

  • A monoclonal antibody which detects a cell surface antigen on murine embryonal carcinoma and early mouse embryo stages may recognize a carbohydrate determinant involving alpha-linked galactose.

    abstract：:A rat monoclonal antibody (5D4) apparently specific for murine embryonal carcinoma cells and cells of the pre-implantation embryo is described. It does not react with differentiated cells from a variety of different tumours and tissues of the mouse. Biochemical studies show that the molecules that carry 5D4 antigen ar...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(83)90012-8

    authors： Stern PL,Gilbert P,Heath JK,Furth M

    更新日期：1983-05-01 00:00:00

  • Isolation of a sperm autoantigen from the kidneys of vasectomized rabbits.

    abstract：:The rabbit sperm membrane autoantigen, RSA-1, has been isolated from the kidneys of bilaterally vasectomized rabbits. Using detergent extraction the IgG-RSA-1 complex in the kidney was isolated first on an anti-IgG immunocolumn. The eluted IgG-RSA-1 complex was then applied to an anti-sperm autoantibody column which b...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/0165-0378(84)90006-8

    authors： O'Rand MG,Porter JP,Alexander NJ

    更新日期：1984-02-01 00:00:00

  • Constitutive or induced elevated levels of L-carnitine correlate with the cytokine and cellular profile of endometriosis.

    abstract：:During the past decade, accumulated evidence indicates an association between endometriosis and an alteration of humoral and cell-mediated immunity. While the role of L-carnitine in the regulation of energy metabolism is well established, it is only recently that L-carnitine has been recognized to modify the immune re...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2004.12.002

    authors： Dionyssopoulou E,Vassiliadis S,Evangeliou A,Koumantakis EE,Athanassakis I

    更新日期：2005-04-01 00:00:00

  • Soluble HLA-G and control of angiogenesis.

    abstract：:In this review, we summarize the results of a number of our recent in vitro and in vivo experiments demonstrating that, in addition to the immunoregulatory functions, soluble HLA-G molecules also affect endothelial cell activity. We have found that soluble HLA-G1 (also designated HLA-G5) inhibits endothelial cell prol...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.jri.2007.03.007

    authors： Le Bouteiller P,Fons P,Herault JP,Bono F,Chabot S,Cartwright JE,Bensussan A

    更新日期：2007-12-01 00:00:00

  • Expression and localization of cell adhesion molecules in human fetal membranes during parturition.

    abstract：:There is increasing evidence to support the view that human parturition represents an inflammatory process. We have previously demonstrated that parturition is associated with leukocyte invasion and pro-inflammatory cytokine production in the cervix and myometrium. Furthermore, we have shown that several cell adhesion...

    journal_title：Journal of reproductive immunology

    pub_type： 杂志文章

    doi：10.1016/j.jri.2004.04.003

    authors： Osman I,Crawford M,Jordan F,Young A,Norman J,Thomson A

    更新日期：2004-08-01 00:00:00