Abstract:
:Human peripheral blood leukocytes, activated by phorbol myristate acetate, disrupt canine sarcoplasmic reticulum calcium transport, in vitro, by an oxygen-derived free radical mechanism. Activated leukocytes significantly depress Ca++ uptake activity and Ca++ -stimulated, Mg++ -dependent ATPase activity. The depression is completely inhibited by sodium-azide (0.1 mM) or the combination of superoxide dismutase (10 micrograms/ml) and catalase (10 micrograms/ml). Exogenous hydrogen peroxide (0.441-4.41 mM) uncoupled Ca++ uptake activity from ATP hydrolysis, and this effect was inhibited by catalase. Mannitol alone did not inhibit the effects of activated leukocytes, but superoxide plus mannitol (20-100 mM) resulted in normal ATPase activity, while Ca++ uptake remained depressed. In the presence of indomethacin and ibuprofen, activated leukocytes depressed Ca++ uptake and had no effect on ATPase activity. 2-Amino-methyl-4-t-butyl-6-iodophenol (MK-447) further depressed Ca++ uptake and partially inhibited the effect on ATPase activity. Indomethacin plus catalase completely inhibited the effects of activated leukocytes on cardiac sarcoplasmic reticulum. We conclude, first, that activated leukocytes depress canine cardiac sarcoplasmic reticulum Ca++ transport by an oxygen-free radical mechanism with the generation of hydrogen peroxide and hydroxyl radical. In addition to the classical membrane NADPH oxidase system, significant oxygen radical generation can occur through the cyclooxygenase pathway of arachidonic acid metabolism, and seems to be responsible for the generation of the hydroxyl radical.
journal_name
Circ Resjournal_title
Circulation researchauthors
Rowe GT,Manson NH,Caplan M,Hess MLdoi
10.1161/01.res.53.5.584subject
Has Abstractpub_date
1983-11-01 00:00:00pages
584-91issue
5eissn
0009-7330issn
1524-4571journal_volume
53pub_type
杂志文章abstract::Nonlaminar flow is a major predisposing factor to atherosclerosis. Yet little is known regarding hemodynamic gene regulation in disease-prone areas of the vascular tree in vivo. We have determined spatial patterns of expression of endothelial cell receptors in the arterial tree and of reporter gene constructs in trans...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000111803.92923.D6
更新日期:2004-02-20 00:00:00
abstract::High sodium intake (HNa) increases brain ouabainlike activity (OLA) in rats. In spontaneously hypertensive rats (SHR), HNa exaggerates development of hypertension and pressor and sympathoexcitatory responses to stress. To investigate whether dietary sodium-induced changes in brain OLA play a functional role, responses...
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更新日期:2010-02-19 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.76.2.199
更新日期:1995-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-06-08 00:00:00
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pub_type: 杂志文章
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更新日期:1998-05-04 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.248880
更新日期:2011-09-02 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.48.4.510
更新日期:1981-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-07-18 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.69.5.1171
更新日期:1991-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1985-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000155964.34150.F7
更新日期:2005-02-18 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.67.3.780
更新日期:1990-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.RES.0000171901.40952.0d
更新日期:2005-06-24 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.46.4.470
更新日期:1980-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.71.4.992
更新日期:1992-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.109.204040
更新日期:2009-10-23 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000149564.49410.0d
更新日期:2004-11-26 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.266445
更新日期:2012-08-03 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2010-10-29 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1987-07-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2014-05-09 00:00:00
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pub_type: 杂志文章
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更新日期:2009-03-13 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2005-10-28 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.61.2.244
更新日期:1987-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.60.6.933
更新日期:1987-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.39.1.106
更新日期:1976-07-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.249482
更新日期:2012-01-20 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.240150
更新日期:2011-09-30 00:00:00