Oxidized phospholipids induce type VIII collagen expression and vascular smooth muscle cell migration.

Abstract:

:Phenotypic switching of vascular smooth muscle cells (VSMCs) is known to play a critical role in the development of atherosclerosis. However, the factors present within lesions that mediate VSMC phenotypic switching are unclear. Oxidized phospholipids (OxPLs), including 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine (POVPC), are active components of minimally modified low density lipoprotein and have been previously shown to induce multiple proatherogenic events in endothelial cells and macrophages, but their effects on VSMCs have been largely unexplored until recently. We previously showed that OxPLs induced phenotypic switching of VSMCs, including suppression of SMC differentiation marker genes. The goal of the present studies was to test the hypothesis that OxPLs alter extracellular matrix production and VSMC migration. Results showed that POVPC activated expression of several extracellular matrix proteins in VSMC. POVPC increased expression of type VIII collagen alpha1 chain (Col8a1) mRNA in cultured VSMCs and in vivo in rat carotid arteries by 9-fold and 4-fold, respectively. POVPC-induced activation of Col8a1 gene expression was reduced by small interfering RNA-mediated suppression of Krüppel-like factor 4 (Klf4) and Sp1, and was abolished in Klf4-knockout VSMCs. POVPC increased Klf4 binding to the Col8a1 gene promoter both in vivo in rat carotid arteries and in cultured VSMCs based on chromatin immunoprecipitation assays. Moreover, POVPC-induced VSMC migration was markedly reduced in Klf4- or type VIII collagen-knockout VSMCs. Given evidence that OxPLs are present within atherosclerotic lesions, it is interesting to suggest that OxPL-induced changes in VSMC phenotype may contribute to the pathogenesis of atherosclerosis at least in part through changes in extracellular matrix composition.

journal_name

Circ Res

journal_title

Circulation research

authors

Cherepanova OA,Pidkovka NA,Sarmento OF,Yoshida T,Gan Q,Adiguzel E,Bendeck MP,Berliner J,Leitinger N,Owens GK

doi

10.1161/CIRCRESAHA.108.186064

subject

Has Abstract

pub_date

2009-03-13 00:00:00

pages

609-18

issue

5

eissn

0009-7330

issn

1524-4571

pii

CIRCRESAHA.108.186064

journal_volume

104

pub_type

杂志文章
  • Duration and Life-Stage of Antibiotic Use and Risks of All-Cause and Cause-Specific Mortality: Prospective Cohort Study.

    abstract::Rationale: The overuse of antibiotics has been an important clinical issue, and antibiotic exposure is linked to alterations in gut microbiota, which has been related to risks of various chronic diseases such as cardiovascular disease and cancer. Also, duration of antibiotic exposure may be a risk factor of premature ...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/CIRCRESAHA.119.315279

    authors: Heianza Y,Ma W,Li X,Cao Y,Chan AT,Rimm EB,Hu FB,Rexrode KM,Manson JE,Qi L

    更新日期:2020-01-31 00:00:00

  • Targeting CCR2 or CD18 inhibits experimental in-stent restenosis in primates: inhibitory potential depends on type of injury and leukocytes targeted.

    abstract::A central role for leukocytes in neointimal hyperplasia after arterial injury is suspected. However, the relative importance of neutrophils and monocytes in balloon or stent-induced injury are not well understood, and mechanistic targeting of leukocyte recruitment or function is crude. We determined the temporal and s...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/hh0402.105956

    authors: Horvath C,Welt FG,Nedelman M,Rao P,Rogers C

    更新日期:2002-03-08 00:00:00

  • Muscarinic-activated K+ current in bovine aortic endothelial cells.

    abstract::Isolated, cultured arterial endothelial cells express an acetylcholine (ACh)-activated K+ current in addition to an inward rectifier current whose conductance is unaffected by ACh. The cholinergic K+ current is specifically blocked by atropine (1 microM) and shows single saturation kinetics with ACh (half-maximal stim...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.62.6.1059

    authors: Olesen SP,Davies PF,Clapham DE

    更新日期:1988-06-01 00:00:00

  • Plasma prorenin in normal, hypertensive, and anephric subjects and its effect on renin measurements.

    abstract::We have previously shown that a cryoactivatable inactive form of renin, which we have tentatively termed prorenin, occurs in human plasma. Plasma prorenin is measured by subtracting the endogenous plasma renin activity (PRA) from the total renin activity measured after cryoactivation. Cryoactivation is accomplished by...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:

    authors: Sealey JE,Moon C,Laragh JH,Atlas SA

    更新日期:1977-05-01 00:00:00

  • Protease nexin-1 interacts with thrombomodulin and modulates its anticoagulant effect.

    abstract::The endothelial cell membrane glycoprotein thrombomodulin (TM) plays a critical role in the regulation of coagulation. TM is an essential cofactor in protein C activation by thrombin, and a direct inhibitor of thrombin-induced platelet activation and fibrinogen clotting. Protease nexin-1 (PN-1) is a serpin synthesized...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000265066.92923.ee

    authors: Bouton MC,Venisse L,Richard B,Pouzet C,Arocas V,Jandrot-Perrus M

    更新日期:2007-04-27 00:00:00

  • Gestational changes in pulmonary converting enzyme activity in the fetal rabbit.

    abstract::Changes in angiotensin-converting enzyme were measured in the lungs of fetal rabbits isolated and perfused in situ at varying ages from 22 days gestation to 7 days of age under controlled conditions of flow, pH, and temperature. Enzyme activity was assessed by infusing bradykinin or angiotensin I in Krebs-Henseleit so...

    journal_title:Circulation research

    pub_type: 杂志文章,收录出版

    doi:10.1161/01.res.43.5.705

    authors: Stalcup SA,Pang LM,Lipset JS,Odya CE,Goodfriend TL,Mellins RB

    更新日期:1978-11-01 00:00:00

  • Mechanisms of plaque formation and rupture.

    abstract::Atherosclerosis causes clinical disease through luminal narrowing or by precipitating thrombi that obstruct blood flow to the heart (coronary heart disease), brain (ischemic stroke), or lower extremities (peripheral vascular disease). The most common of these manifestations is coronary heart disease, including stable ...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.114.302721

    authors: Bentzon JF,Otsuka F,Virmani R,Falk E

    更新日期:2014-06-06 00:00:00

  • Ischemic preconditioning reduces infarct size in swine myocardium.

    abstract::We evaluated the hypothesis that stunning swine myocardium with brief ischemia reduces oxygen demand in the stunned region and increases tolerance of myocardium to longer periods of ischemia. Wall function was quantified with ultrasonic crystals aligned to measure wall thickening, and stunning was achieved with two cy...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.66.4.1133

    authors: Schott RJ,Rohmann S,Braun ER,Schaper W

    更新日期:1990-04-01 00:00:00

  • Blood pressure and vascular resistance in genetically hypertensive rats treated at birth with 6-hydroxydopamine.

    abstract::Genetically hypertensive (GH) rats of the New Zealand strain and normotensive (N) rats were sympathectomized from birth with 6-hydroxydopamine (100 mg/kg,s.c, on alternate days, seven treatments). In adult treated rats from each strain (GHTr and NTr), blood pressure was lower than normal. Functional tests and electron...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.43.2.293

    authors: Clark DW,Jones DR,Phelan EL,Devine CE

    更新日期:1978-08-01 00:00:00

  • Crosstalk of beta-adrenergic receptor subtypes through Gi blunts beta-adrenergic stimulation of L-type Ca2+ channels in canine heart failure.

    abstract::The mechanisms underlying the blunted contractile response to beta-adrenergic receptor (beta-AR) stimulation in heart failure (HF) are incompletely understood, especially with regard to beta-AR subtype-specific regulation of L-type Ca2+ channels. We evaluated the impact of HF induced by pacing tachycardia on beta-AR r...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000181160.31851.05

    authors: He JQ,Balijepalli RC,Haworth RA,Kamp TJ

    更新日期:2005-09-16 00:00:00

  • Human nonmuscle myosin heavy chains are encoded by two genes located on different chromosomes.

    abstract::We report the cloning of cDNAs encoding two different human nonmuscle myosin heavy chains designated NMMHC-A and NMMHC-B. The mRNAs encoding NMMHC-A and NMMHC-B are both 7.5 kb in size but are shown to be the products of different genes, which are localized to chromosome 22q11.2 and chromosome 17q13, respectively. In ...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.69.2.530

    authors: Simons M,Wang M,McBride OW,Kawamoto S,Yamakawa K,Gdula D,Adelstein RS,Weir L

    更新日期:1991-08-01 00:00:00

  • Antibody and complement in transplant vasculopathy.

    abstract::Advances in immunosuppression have decreased the incidence of acute rejection, but the development of vasculopathy in the coronary arteries of transplants continues to limit the survival of cardiac allografts. Transplant vasculopathy has also been referred to as accelerated graft arteriosclerosis because it has featur...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/01.RES.0000255032.33661.88

    authors: Wehner J,Morrell CN,Reynolds T,Rodriguez ER,Baldwin WM 3rd

    更新日期:2007-02-02 00:00:00

  • Muscle stiffness determined from canine left ventricular pressure-volume curves.

    abstract::We measured pressure-volume curves in nine excised dog ventricles and stress-strain curves in two to five muscle specimens from each ventricle to verify a derived formula that relates muscle stiffness to the ventricular pressure-volume curve. The assumptions underlying this formula are: (1) the ventricle is a uniform ...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.37.6.787

    authors: Glantz SA,Kernoff RS

    更新日期:1975-12-01 00:00:00

  • Neuropeptide Y as a putative modulator of the vagal effects on heart rate.

    abstract::Neuropeptide Y is stored in sympathetic nerve terminals throughout the heart and has direct and indirect effects on cardiac function. Although neuropeptide Y has been shown to be released upon intense (16-30 Hz) cardiac sympathetic stimulation, we sought to determine whether effective quantities of neuropeptide Y were...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.64.5.882

    authors: Warner MR,Levy MN

    更新日期:1989-05-01 00:00:00

  • Genetic manipulation of cardiac K(+) channel function in mice: what have we learned, and where do we go from here?

    abstract::In the mammalian myocardium, potassium (K(+)) channels control resting potentials, action potential waveforms, automaticity, and refractory periods and, in most cardiac cells, multiple types of K(+) channels that subserve these functions are expressed. Molecular cloning has revealed the presence of a large number of K...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/hh2301.100349

    authors: Nerbonne JM,Nichols CG,Schwarz TL,Escande D

    更新日期:2001-11-23 00:00:00

  • Metabolic coupling of glutathione between mouse and quail cardiac myocytes and its protective role against oxidative stress.

    abstract::Cultured quail myocytes were much more resistant to H2O2 toxicity than cultured mouse myocytes. The intracellular concentration of glutathione ([GSH]i) and the activity of gamma-glutamylcysteine synthetase (gamma-GCS) in quail heart cells were about five and three times higher, respectively, than in mouse heart cells,...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.74.5.806

    authors: Nakamura TY,Yamamoto I,Kanno Y,Shiba Y,Goshima K

    更新日期:1994-05-01 00:00:00

  • Mechanism of adenosine-induced inhibition of calcium current in guinea pig ventricular cells.

    abstract::The mechanism of adenosine-induced inhibition of Ca2+ currents was studied by recording single-channel Ca2+ currents from cell-attached patches on isolated guinea pig ventricular cells with pipettes containing 50 or 100 mM Ba2+. Numerous 100-msec depolarization steps were applied repetitively at 2 Hz from the resting ...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.67.5.1134

    authors: Kato M,Yamaguchi H,Ochi R

    更新日期:1990-11-01 00:00:00

  • Active oxygen species stimulate vascular smooth muscle cell growth and proto-oncogene expression.

    abstract::Vascular smooth muscle cells (VSMCs) proliferate in response to arterial injury. Recent findings suggest that, in addition to platelet-derived growth factors, growth factors from inflammatory cells and endothelial cells at the site of injury may contribute to VSMC proliferation. We hypothesized that a common mechanism...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.70.3.593

    authors: Rao GN,Berk BC

    更新日期:1992-03-01 00:00:00

  • Vascular endothelial growth factor upregulates the expression of matrix metalloproteinases in vascular smooth muscle cells: role of flt-1.

    abstract::Vascular endothelial growth factor (VEGF) is a critical regulator of angiogenesis that stimulates proliferation, migration, and proteolytic activity of endothelial cells. Although the mitogenic activity of VEGF is endothelial cell specific, recent reports indicate VEGF is able to stimulate chemotaxis and tissue factor...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.83.8.832

    authors: Wang H,Keiser JA

    更新日期:1998-10-19 00:00:00

  • Electrophysiological study of cardiovascular neurons in the rostral ventrolateral medulla in rats.

    abstract::In urethane-anesthetized rats, electrophysiological recordings of spontaneously active neurons in the vasopressor area of the rostral ventrolateral medulla were analyzed for participation in cardiovascular regulation. A total of 138 units were found which were inhibited by transient increases in mean arterial pressure...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.56.3.359

    authors: Brown DL,Guyenet PG

    更新日期:1985-03-01 00:00:00

  • RNA Splicing: Regulation and Dysregulation in the Heart.

    abstract::RNA splicing represents a post-transcriptional mechanism to generate multiple functional RNAs or proteins from a single transcript. The evolution of RNA splicing is a prime example of the Darwinian function follows form concept. A mutation that leads to a new mRNA (form) that encodes for a new functional protein (func...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.115.307872

    authors: van den Hoogenhof MM,Pinto YM,Creemers EE

    更新日期:2016-02-05 00:00:00

  • Identification of an angiotensin receptor in rabbit renomedullary interstitial cells in tissue culture. Correlation with prostaglandin biosynthesis.

    abstract::High affinity binding sites of angiotensin II (A II) have been characterized in rabbit renomedullary interstitial cells in tissue culture. Binding was rapid, specific, and saturable. Scatchard analysis of steady state saturation binding data at 22 degrees C indicated a single binding site with an equilibrium dissociat...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.46.6.802

    authors: Brown CA,Zusman RM,Haber E

    更新日期:1980-06-01 00:00:00

  • Direct visualization of lipid deposition and reverse lipid transport in a perfused artery : roles of VLDL and HDL.

    abstract::The major goal of this study was to determine the interactions of VLDL surface and core lipids with the artery wall. We first demonstrated in vitro that surface lipid in VLDL could be traced using the phospholipid-like fluorescent probe 1,1'-dioctadecyl-3,3, 3',3'-tetramethyl-indocarbocyanine (DiI). The core of VLDL p...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.86.7.768

    authors: Rutledge JC,Mullick AE,Gardner G,Goldberg IJ

    更新日期:2000-04-14 00:00:00

  • Tissue- and species-specific expression of inhibitory guanine nucleotide-binding proteins. Cloning of a full-length complementary DNA from canine heart.

    abstract::Three different isoforms of the inhibitory guanine nucleotide-binding protein alpha-subunit (Gi alpha) have been cloned. However, little information on the cardiac-specific expression of these isoforms is available. The deduced amino acid sequence of a full-length Gi alpha 2 complementary DNA from a canine ventricular...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.65.4.1136

    authors: Holmer SR,Stevens S,Homcy CJ

    更新日期:1989-10-01 00:00:00

  • Animal model that mimics atherosclerotic plaque rupture.

    abstract::Atherosclerotic plaque rupture is the main cause of coronary thrombosis and myocardial infarcts. Currently, there is no animal model of plaque disruption. We have developed a rabbit model in which an atherosclerotic plaque can be ruptured at will after an inflatable balloon becomes embedded into the plaque. Furthermor...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.83.7.705

    authors: Rekhter MD,Hicks GW,Brammer DW,Work CW,Kim JS,Gordon D,Keiser JA,Ryan MJ

    更新日期:1998-10-05 00:00:00

  • Protein kinase C activation contributes to microvascular barrier dysfunction in the heart at early stages of diabetes.

    abstract::The functional disturbance of microvasculature is recognized as an initiating mechanism that underlies the development of various diabetic complications. Although a causal relationship between microvascular leakage and tissue damage has been well documented in diabetic kidneys and eyes, there is a lack of information ...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.87.5.412

    authors: Yuan SY,Ustinova EE,Wu MH,Tinsley JH,Xu W,Korompai FL,Taulman AC

    更新日期:2000-09-01 00:00:00

  • Control of coronary vascular tone by nitric oxide.

    abstract::A specific difference-spectrophotometric method was used to measure nitric oxide (NO) release into the coronary effluent perfusate of isolated, constant-flow-perfused guinea pig hearts. Authentic NO applied into the coronary circulation decreased vascular resistance dose dependently and enhanced coronary release of cy...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.66.6.1561

    authors: Kelm M,Schrader J

    更新日期:1990-06-01 00:00:00

  • Heart failure gene therapy: the path to clinical practice.

    abstract::Gene therapy, aimed at the correction of key pathologies being out of reach for conventional drugs, bears the potential to alter the treatment of cardiovascular diseases radically and thereby of heart failure. Heart failure gene therapy refers to a therapeutic system of targeted drug delivery to the heart that uses fo...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.113.300269

    authors: Pleger ST,Brinks H,Ritterhoff J,Raake P,Koch WJ,Katus HA,Most P

    更新日期:2013-08-30 00:00:00

  • Cholinergic constriction in the general circulation and its role in coronary artery spasm.

    abstract::The release of acetylcholine from autonomic nerves in those tissues that receive a cholinergic innervation is widely believed to dilate blood vessels. Exogenously administered acetylcholine in vivo does dilate vascular beds and produce hypotension; however, this latter effect is indirect and probably the result of lib...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/01.res.65.2.237

    authors: Kalsner S

    更新日期:1989-08-01 00:00:00

  • Decreased cardiac concentration of cGMP kinase in hypertensive animals. An index for cardiac vascularization?

    abstract::Cyclic GMP (cGMP) kinase is intimately involved in the regulation of vascular smooth muscle tone. Its tissue concentration was determined in normotensive and hypertensive rats by use of monospecific anti-cGMP kinase antibodies. Hearts of spontaneously hypertensive rats and renovascular (Goldblatt II) hypertensive rats...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.65.5.1361

    authors: Ecker T,Göbel C,Hullin R,Rettig R,Seitz G,Hofmann F

    更新日期:1989-11-01 00:00:00