Abstract:
:Strain B(8-11) has been found to be very sensitive to postirradiation DNA degradation. Up to 98% of the DNA is degraded at optimum doses. The amount of residual DNA correlates with the retention of colony-forming ability (CFA). Studies of rates of degradation as a function of dose agree with the concept that a degrading lesion causes a definite rate of degradation and that increased numbers of lesions produce proportionally faster rates. By observing the burst size of T7 phage which uses host DNA it has been established that DNA degradation occurs in an all-or-nothing fashion in a unit which is present two or three times per cell. Degradation is enzymatic and the enzyme system is already present in the cell as evidenced by the rapid onset of degradation. DNA synthesis continues in cells that have lost some chromosomes by degradation. Single-cell division patterns show that recovery from "sublethal" damage can occur even in this sensitive cell. Recovery in preirradiation oxygenated cells differs from that in nitrogenated cells.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Pollard EC,Tilberg Adoi
10.1016/S0006-3495(72)86076-4subject
Has Abstractpub_date
1972-02-01 00:00:00pages
133-56issue
2eissn
0006-3495issn
1542-0086pii
S0006-3495(72)86076-4journal_volume
12pub_type
杂志文章abstract::The kinetics of oxygen geminate binding with the taxonomic substates of MbO2 are reported. The maximum entropy method was used to analyze the rebinding kinetics of MbCO and MbO2 monitored in the Soret. The resulting rate distributions were found to consist of a small number of overlapping bands. A global parametric fi...
journal_title:Biophysical journal
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pub_type: 杂志文章
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更新日期:2011-04-20 00:00:00
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pub_type: 杂志文章
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更新日期:2012-09-19 00:00:00
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pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-03-04 00:00:00
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pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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更新日期:2016-01-19 00:00:00
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pub_type: 杂志文章
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更新日期:2015-05-05 00:00:00
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更新日期:2012-05-02 00:00:00
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更新日期:1980-01-01 00:00:00
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更新日期:2006-02-15 00:00:00
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pub_type: 杂志文章
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