Just a Flexible Linker? The Structural and Dynamic Properties of CBP-ID4 Revealed by NMR Spectroscopy.

abstract：:In the photoactive yellow protein, PYP, both Glu46 and Tyr42 form hydrogen bonds to the phenolic OH group of the p-hydroxycinnamoyl chromophore. Previous work on replacement of the carboxyl group of Glu46 by an amide group (Glu46Gln) has shown that changing the nature of this hydrogen bond has a minimal effect on the ...

journal_title：Biophysical journal

authors： Devanathan S,Lin S,Cusanovich MA,Woodbury N,Tollin G

更新日期：2001-10-01 00:00:00

abstract：:Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols can be designed. N...

journal_title：Biophysical journal

authors： Henrich E,Sörmann J,Eberhardt P,Peetz O,Mezhyrova J,Morgner N,Fendler K,Dötsch V,Wachtveitl J,Bernhard F,Bamann C

更新日期：2017-09-19 00:00:00

abstract：:Fatty acid binding proteins play an important role in the transportation of fatty acids. Despite intensive studies, how fatty acids enter the protein cavity for binding is still controversial. Here, a gap-closed variant of human intestinal fatty acid binding protein was generated by mutagenesis, in which the gap is lo...

journal_title：Biophysical journal

authors： Xiao T,Lu Y,Fan JS,Yang D

更新日期：2020-01-21 00:00:00

abstract：:The structural properties of a crucial transmembrane helix for proton translocation in vacuolar ATPase are studied using double site-directed spin-labeling combined with electron spin resonance (ESR) (or electron paramagnetic resonance) and circular dichroism spectroscopy in sodium dodecyl sulfate micelles. For this p...

journal_title：Biophysical journal

authors： Vos WL,Vermeer LS,Hemminga MA

更新日期：2007-01-01 00:00:00

abstract：:Proteins are not rigid molecules, but exhibit internal motions on timescales ranging from femto- to milliseconds and beyond. In solution, proteins also experience global translational and rotational motions, sometimes on timescales comparable to those of the internal fluctuations. The possibility that internal and glo...

journal_title：Biophysical journal

authors： Hamaneh MB,Zhang L,Buck M

更新日期：2011-07-06 00:00:00

abstract：:Many organisms are able to survive subzero temperatures at which bodily fluids would normally be expected to freeze. These organisms have adapted to these lower temperatures by synthesizing antifreeze proteins (AFPs), capable of binding to ice, which make further growth of ice energetically unfavorable. To date, the s...

journal_title：Biophysical journal

authors： Graether SP,Slupsky CM,Davies PL,Sykes BD

更新日期：2001-09-01 00:00:00

abstract：:The UV-visible absorption and magnetic circular dichroism (MCD) spectra of the ferric, ferrous, CO-ligated forms and kinetic photolysis intermediates of the tetraheme electron-transfer protein cytochrome c3 (Cc3) are reported. Consistent with bis-histidinyl axial coordination of the hemes in this Class III c-type cyto...

journal_title：Biophysical journal

authors： O'Connor DB,Goldbeck RA,Hazzard JH,Kliger DS,Cusanovich MA

更新日期：1993-10-01 00:00:00

abstract：:The piezoelectric properties of single collagen type I fibrils in fascia were imaged with sub-20 nm spatial resolution using piezoresponse force microscopy. A detailed analysis of the piezoresponse force microscopy signal in controlled tip-fibril geometry revealed shear piezoelectricity parallel to the fibril axis. Th...

journal_title：Biophysical journal

authors： Harnagea C,Vallières M,Pfeffer CP,Wu D,Olsen BR,Pignolet A,Légaré F,Gruverman A

更新日期：2010-06-16 00:00:00

abstract：:The pore-forming alpha 1 subunit of L-type calcium (Ca2+) channels is the molecular target of Ca2+ channel blockers such as phenylalkylamines (PAAs). Association and dissociation rates of (-)devapamil were compared for a highly PAA-sensitive L-type Ca2+ channel chimera (Lh) and various class A Ca2+ channel mutants. Th...

journal_title：Biophysical journal

authors： Degtiar VE,Aczél S,Döring F,Timin EN,Berjukow S,Kimball D,Mitterdorfer J,Hering S

更新日期：1997-07-01 00:00:00

abstract：:One theory of freezing damage suggests that slowly cooled cells are killed by being exposed to increasing concentrations of electrolytes as the suspending medium freezes. A corollary to this view is that protective additives such as glycerol protect cells by acting colligatively to reduce the electrolyte concentration...

journal_title：Biophysical journal

authors： Rall WF,Mazur P,Souzu H

更新日期：1978-07-01 00:00:00

abstract：:In the absence of atomic structures of high-density lipoproteins in their lipid-bound states, many molecular models have been produced based on experimental data. Using molecular dynamics, we show that a recently proposed "solar-flares" model of discoidal high-density lipoprotein is implausible. Our simulations show a...

journal_title：Biophysical journal

authors： Shih AY,Sligar SG,Schulten K

更新日期：2008-06-01 00:00:00

abstract：:Cytochrome c3 (cyt c3) can mediate electron transport across phosphatidylcholine (PC)/cardiolipin (CL) and PC/phosphatidylglycerol (PG) membranes. A two-molecule process is involved in the electron transport across PC/CL membranes in the liquid-crystalline state. In contrast, a single-molecule process dominates the el...

journal_title：Biophysical journal

authors： Kim SM,Yamamoto T,Todokoro Y,Takayama Y,Fujiwara T,Park JS,Akutsu H

更新日期：2006-01-15 00:00:00

abstract：:KL(4) is a 21-residue functional peptide mimic of lung surfactant protein B, an essential protein for lowering surface tension in the alveoli. Its ability to modify lipid properties and restore lung compliance was investigated with circular dichroism, differential scanning calorimetry, and solid-state NMR spectroscopy...

journal_title：Biophysical journal

authors： Antharam VC,Elliott DW,Mills FD,Farver RS,Sternin E,Long JR

更新日期：2009-05-20 00:00:00

abstract：:The time correlation function of the shape fluctuations of large (greater than 10 micron), cylindrical, hydrated, phospholipid-membrane vesicles consisting of one bimolecular layer was measured. The restoring force of the membrane was due to the excess curvature of a membrane element. A value for the curvature elastic...

journal_title：Biophysical journal

authors： Schneider MB,Jenkins JT,Webb WW

更新日期：1984-05-01 00:00:00

abstract：:A number of important pluripotency regulators, including the transcription factor Nanog, are observed to fluctuate stochastically in individual embryonic stem cells. By transiently priming cells for commitment to different lineages, these fluctuations are thought to be important to the maintenance of, and exit from, p...

journal_title：Biophysical journal

authors： Smith RCG,Stumpf PS,Ridden SJ,Sim A,Filippi S,Harrington HA,MacArthur BD

更新日期：2017-06-20 00:00:00

abstract：:The theoretical basis of an optical microscope technique to image dynamically scattered light fluctuation decay rates (dynamic light scattering microscopy) is developed. It is shown that relative motions between scattering centers even smaller than the optical resolution of the microscope are sufficient to produce sig...

journal_title：Biophysical journal

authors： Dzakpasu R,Axelrod D

更新日期：2004-08-01 00:00:00

abstract：:Two-color spatio-temporal image cross-correlation spectroscopy (STICCS) is a new, to our knowledge, image analysis method that calculates space-time autocorrelation and cross-correlation functions from fluorescence intensity fluctuations. STICCS generates cellular flow and diffusion maps that reveal interactions and c...

journal_title：Biophysical journal

authors： Toplak T,Pandzic E,Chen L,Vicente-Manzanares M,Horwitz AR,Wiseman PW

更新日期：2012-10-17 00:00:00

abstract：:Multiple molecular dynamics simulations of bacterioopsin pulling from its C-terminus show that its alpha-helices unfold individually. In the first metastable state observed in the simulations, helix G is unfolded at its C-terminal segment while the rest of helix G (residues 200-216) is folded and opposes resistance be...

journal_title：Biophysical journal

authors： Seeber M,Fanelli F,Paci E,Caflisch A

更新日期：2006-11-01 00:00:00

abstract：:Elastin-derived peptides are released from the extracellular matrix remodeling by numerous proteases and seem to regulate many biological processes, notably cancer progression. The canonical elastin peptide is VGVAPG, which harbors the XGXXPG consensus pattern, allowing interaction with the elastin receptor complex lo...

journal_title：Biophysical journal

authors： Hernández B,Crowet JM,Thiery J,Kruglik SG,Belloy N,Baud S,Dauchez M,Debelle L

更新日期：2020-06-02 00:00:00

abstract：:We elucidate the physics of protein dynamical transition via 10-100-ns molecular dynamics simulations at temperatures spanning 160-300 K. By tracking the energy fluctuations, we show that the protein dynamical transition is marked by a crossover from nonstationary to stationary processes that underlie the dynamics of ...

journal_title：Biophysical journal

authors： Okan OB,Atilgan AR,Atilgan C

更新日期：2009-10-07 00:00:00

abstract：:There is increasing evidence that UVA radiation, which makes up approximately 95% of the solar UV light reaching the Earth's surface and is also commonly used for cosmetic purposes, is genotoxic. However, in contrast to UVC and UVB, the mechanisms by which UVA produces various DNA lesions are still unclear. In additio...

journal_title：Biophysical journal

authors： Jiang Y,Rabbi M,Kim M,Ke C,Lee W,Clark RL,Mieczkowski PA,Marszalek PE

更新日期：2009-02-01 00:00:00

abstract：:We have studied the wavelength dependence of retinal Schiff base absorbencies on the protonation state of the chromophore at the multiconfigurational level of theory using second order perturbation theory (CASPT2) within an atomic natural orbital basis set on MP2 optimized geometries. Quantitative agreement between ca...

journal_title：Biophysical journal

authors： Sekharan S,Weingart O,Buss V

更新日期：2006-07-01 00:00:00

abstract：:Photoinduced electron transfer in photosystem I (PS I) proceeds from the excited primary electron donor P700 (a chlorophyll a dimer) via the primary acceptor A0 (chlorophyll a) and the secondary acceptor A1 (phylloquinone) to three [4Fe-4S] clusters, Fx, FA, and FB. Prereduction of the iron-sulfur clusters blocks elec...

journal_title：Biophysical journal

authors： Polm M,Brettel K

更新日期：1998-06-01 00:00:00

abstract：:Time-resolved photovoltage measurements were performed with the acid-blue (bR605A) and acid-purple (bR565A) forms of bacteriorhodopsin (bR) in the time range from 25 ns to 100 s. The bR605A and bR565A pigments were formed by titration with H2SO4 in the absence and presence of 150 mM KCI, respectively. Qualitatively th...

journal_title：Biophysical journal

authors： Moltke S,Heyn MP

更新日期：1995-11-01 00:00:00

abstract：:We combine ensemble and single-molecule spectroscopy to gain insight into the energy transfer between chlorophylls (Chls) in peridinin-chlorophyll-protein (PCP) complexes reconstituted with Chl a, Chl b, as well as both Chl a and Chl b. The main focus is the heterochlorophyllous system (Chl a/b-N-PCP), and reference i...

journal_title：Biophysical journal

authors： Mackowski S,Wörmke S,Brotosudarmo TH,Jung C,Hiller RG,Scheer H,Bräuchle C

更新日期：2007-11-01 00:00:00

abstract：:The conformational states of Escherichia coli Rep helicase undergoing ATP hydrolysis while bound to a partial-duplex DNA (pdDNA) were studied using single-molecule FRET. Crystallographic studies showed that Rep bound to single-stranded DNA can exist in open and closed conformations that differ in the orientation of th...

journal_title：Biophysical journal

authors： Balci H,Arslan S,Myong S,Lohman TM,Ha T

更新日期：2011-08-17 00:00:00

abstract：:Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggre...

journal_title：Biophysical journal

authors： Donnell JT 2nd,Finegold LX

更新日期：1981-09-01 00:00:00

abstract：:In dialyzed Myxicola axons substitution of heavy water (D2O) externally and internally slows both sodium and potassium kinetics and decreases the maximum conductances. Furthermore, this effect is strongly temperature dependent, the magnitude of the slowing produced by D2O substitution decreasing with increasing temper...

journal_title：Biophysical journal

authors： Schauf CL,Bullock JO

更新日期：1979-08-01 00:00:00

abstract：:The scattering and absorption coefficients of many homogeneous biological tissues such as muscle, skin, white matter in the brain, and dentin are often anisotropically oriented with respect to their bounding interface. In consequence the curves of equal intensity of re-emitted light on the surface of the slab will no ...

journal_title：Biophysical journal

authors： Dudko OK,Weiss GH

更新日期：2005-05-01 00:00:00

abstract：:An experimental procedure is described which consists of cutting the canal duct, inserting a micropipette and administering known volumetric displacements to the cupula. The cupula is made visible by dying the endolymph. Known displacements are administered to the cupula, and the time constant of the return to its equ...

journal_title：Biophysical journal

authors： Grant JW,Van Buskirk WC

更新日期：1976-06-01 00:00:00