STICCS reveals matrix-dependent adhesion slipping and gripping in migrating cells.

abstract：:Enterococcus hirae V1-ATPase is a molecular motor composed of the A3B3 hexamer ring and the central stalk. In association with ATP hydrolysis, three catalytic AB pairs in the A3B3 ring undergo conformational changes, which lead to a 120° rotation of the central stalk. To understand how the conformational changes of th...

journal_title：Biophysical journal

authors： Isaka Y,Ekimoto T,Kokabu Y,Yamato I,Murata T,Ikeguchi M

更新日期：2017-03-14 00:00:00

abstract：:In an attempt to quantitate the physical behavior of biological systems, Fourier analysis has been applied to the respiratory and circulatory systems by a number of investigators. The validity of this application has been questioned on the basis that these systems are nonlinear and not strictly periodic. If these obje...

journal_title：Biophysical journal

authors： Attinger EO,Anné A,McDonald DA

更新日期：1966-05-01 00:00:00

abstract：:During inflammation, flowing leukocytes tether to and roll on vascular surfaces through the association and dissociation of selectin/ligand bonds. The interactions of P- and L- selectins with their respective ligands exhibit catch-slip bonds, such that increasing force initially prolongs and then shortens bond lifetim...

journal_title：Biophysical journal

authors： Wayman AM,Chen W,McEver RP,Zhu C

更新日期：2010-08-09 00:00:00

abstract：:The partial specific volume and adiabatic compressibility of proteins reflect the hydration properties of the solvent-exposed protein surface, as well as changes in conformational states. Reverse micelles, or water-in-oil microemulsions, are protein-sized, optically-clear microassemblies in which hydration can be expe...

journal_title：Biophysical journal

authors： Valdez D,Le Huérou JY,Gindre M,Urbach W,Waks M

更新日期：2001-06-01 00:00:00

abstract：:Bistability is a major mechanism for cellular decision making and usually results from positive feedback in biochemical control systems. Here we show theoretically that bistability between unbound and bound states of adhesion clusters results from positive feedback mediated by structural rather than biochemical proces...

journal_title：Biophysical journal

authors： Erdmann T,Schwarz US

更新日期：2006-09-15 00:00:00

abstract：:Transmissible spongiform encephalopathies are neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein PrP(Sc). Its fragment 106-126 has been reported to maintain most of the pathological features of PrP(Sc), and a role in neurodegeneration has been proposed based on the...

journal_title：Biophysical journal

authors： Henriques ST,Pattenden LK,Aguilar MI,Castanho MA

更新日期：2008-08-01 00:00:00

abstract：:Embryonic and adult fibroblasts can be returned to pluripotency by the expression of reprogramming genes. Multiple lines of evidence suggest that these human induced pluripotent stem (hiPS) cells and human embryonic stem (hES) cells are behaviorally, karyotypically, and morphologically similar. Here we sought to deter...

journal_title：Biophysical journal

authors： Daniels BR,Hale CM,Khatau SB,Kusuma S,Dobrowsky TM,Gerecht S,Wirtz D

更新日期：2010-12-01 00:00:00

abstract：:The functional diversity of gap junction intercellular channels arising from the large number of connexin isoforms is significantly increased by heterotypic interactions between members of this family. This is particularly evident in the rectifying behavior of Cx26/Cx32 heterotypic channels (. Proc. Natl. Acad. Sci. U...

journal_title：Biophysical journal

authors： Suchyna TM,Nitsche JM,Chilton M,Harris AL,Veenstra RD,Nicholson BJ

更新日期：1999-12-01 00:00:00

abstract：:Photoreverse reactions of octopus rhodopsin (Rh) from acid-metarhodopsin (Acid-Meta), which is the final product of the photoreaction of Rh, to Rh were studied by the time-resolved transient absorption and transient grating methods. The time course of the absorption signal showed a rapid change within 500 ns followed ...

journal_title：Biophysical journal

authors： Inoue K,Tsuda M,Terazima M

更新日期：2007-05-15 00:00:00

abstract：:The effects of myosin regulatory light chain (RLC) phosphorylation and strain on adenosine diphosphate (ADP) release from cross-bridges in phasic (rabbit bladder (Rbl)) and tonic (femoral artery (Rfa)) smooth muscle were determined by monitoring fluorescence transients of the novel ADP analog, 3'-deac-eda-ADP (deac-ed...

journal_title：Biophysical journal

authors： Khromov AS,Webb MR,Ferenczi MA,Trentham DR,Somlyo AP,Somlyo AV

更新日期：2004-04-01 00:00:00

abstract：:Green flagellates possess rhodopsin-like photoreceptors involved in control of their behavior via generation of photocurrents across the plasma membrane. Chlamydomonas mutants blocked in retinal biosynthesis are "blind," but they can be rescued by the addition of exogenous retinoids. Photosignaling by chlamyrhodopsin ...

journal_title：Biophysical journal

authors： Govorunova EG,Sineshchekov OA,Gärtner W,Chunaev AS,Hegemann P

更新日期：2001-11-01 00:00:00

abstract：:To investigate the mechanisms by which vesicles of pulmonary surfactant adsorb to an air-liquid interface, we measured the effect of different phospholipids and of their concentration both in the subphase and at the interface on this process. Adsorbing vesicles contained the hydrophobic surfactant proteins mixed with ...

journal_title：Biophysical journal

authors： Walters RW,Jenq RR,Hall SB

更新日期：2000-01-01 00:00:00

abstract：:The effect of a nonuniform solute concentration on the osmotic transport of water through the boundaries of a simple model cell is investigated. A system of two ordinary differential equations is derived for the motion of a single cell in the limit of a fast solute diffusion, and an analytic solution is obtained for o...

journal_title：Biophysical journal

authors： Jaeger M,Carin M,Medale M,Tryggvason G

更新日期：1999-09-01 00:00:00

abstract：:A method for determination of the orientation of adsorbed structure-stable proteins using Total Internal Reflection Fluorescence is outlined. The theory has been elaborated for orientation studies on adsorbed free base cytochrome c, of which the prophyrin can be used as an intrinsic fluorescent label. The ratio of flu...

journal_title：Biophysical journal

authors： Fraaije JG,Kleijn JM,van der Graaf M,Dijt JC

更新日期：1990-05-01 00:00:00

abstract：:To give a realistic representation of the pulse propagation in arteries a theoretical analysis of the wave propagation through a viscous incompressible fluid contained in an initially stressed elastic tube is considered. The tube is assumed to be orthotropic and its longitudinal motion is constrained by a uniformly di...

journal_title：Biophysical journal

authors： Atabek HB

更新日期：1968-05-01 00:00:00

abstract：:By considering how polymer structures are distributed in conformation space, we show that it is possible to quantify the difficulty of structural prediction and to provide a measure of progress for prediction calculations. The critical issue is the probability that a conformation is found within a specified distance o...

journal_title：Biophysical journal

authors： Sullivan DC,Kuntz ID

更新日期：2004-07-01 00:00:00

abstract：:The physical characteristics which govern the water relations of the giant-celled sporangiophore of Phycomyces blakesleeanus were measured with the pressure probe technique and with nanoliter osmometry. These properties are important because they govern water uptake associated with cell growth and because they may in...

journal_title：Biophysical journal

authors： Cosgrove DJ,Ortega JK,Shropshire W Jr

更新日期：1987-03-01 00:00:00

abstract：:Phase-resolved monitoring of photoacoustic signals can provide information about the depth profile of a sample. We describe an application of this principle to determine the depth profiles of ligands and antitumor agents in mammalian cells. Measurements of the in-phase and quadrature components of the photoacoustic sp...

journal_title：Biophysical journal

authors： Narayanan K,Chandani S,Ramakrishna T,Rao CM

更新日期：1997-05-01 00:00:00

abstract：:By using an analogy between the magnetization of a paramagnetic material in an external magnetic field and the electric polarization of the lateral wall of outer hair cells in response to the transmembrane potential, we show that, based on experimental data on the charge transfer across the membrane, it is impossible ...

journal_title：Biophysical journal

authors： Scherer MP,Gummer AW

更新日期：2005-05-01 00:00:00

abstract：:The electrical membrane potential (Vm) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because Vm also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety...

journal_title：Biophysical journal

authors： Mancini L,Terradot G,Tian T,Pu Y,Li Y,Lo CJ,Bai F,Pilizota T

更新日期：2020-01-07 00:00:00

abstract：:Thermodynamically rigorous free energy methods in principle allow the exact computation of binding free energies in biological systems. Here, we use thermodynamic integration together with molecular dynamics simulations of a DNA-protein complex to compute relative binding free energies of a series of mutants of a prot...

journal_title：Biophysical journal

authors： Beierlein FR,Kneale GG,Clark T

更新日期：2011-09-07 00:00:00

abstract：:We used the nonlinear Poisson-Boltzmann equation to calculate electrostatic potentials in the aqueous phase adjacent to model phospholipid bilayers containing mixtures of zwitterionic lipids (phosphatidylcholine) and acidic lipids (phosphatidylserine or phosphatidylglycerol). The aqueous phase (relative permittivity, ...

journal_title：Biophysical journal

authors： Peitzsch RM,Eisenberg M,Sharp KA,McLaughlin S

更新日期：1995-03-01 00:00:00

abstract：:Cholesterol is abundant in plasma membranes and exhibits a variety of interactions throughout the membrane. Chemical potential accounts for thermodynamic consequences of molecular interactions, and quantifies the effective concentration (i.e., activity) of any substance participating in a process. We have developed, t...

journal_title：Biophysical journal

authors： Ayuyan AG,Cohen FS

更新日期：2018-02-27 00:00:00

abstract：:Frog skeletal muscle fibers, mechanically skinned under water-saturated silicone oil, swell upon transfer to aqueous relaxing medium (60 mM KCl; 3 mM MgCl(2); 3 mM ATP; 4 mM EGTA; 20 mM Tris maleate; pH = 7.0; ionic strength 0.15 M). Their cross-sectional areas, estimated with an elliptical approximation, increase 2.3...

journal_title：Biophysical journal

authors： Godt RE,Maughan DW

更新日期：1977-08-01 00:00:00

abstract：:Recently various pathways of human telomere (ht) DNA folding into G-quadruplexes and of ligand binding to these structures have been proposed. However, the key issue as to the nature of forces driving the folding and recognition processes remains unanswered. In this study, structural changes of 22-mer ht-DNA fragment ...

journal_title：Biophysical journal

authors： Bončina M,Hamon F,Islam B,Teulade-Fichou MP,Vesnaver G,Haider S,Lah J

更新日期：2015-06-16 00:00:00

abstract：:Using a recently developed in vitro motility assay, we have demonstrated that local anesthetics directly inhibit myosin-based movement of single actin filaments in a reversible dose-dependent manner. This is the first reported account of the actions of local anesthetics on purified proteins at the molecular level. In ...

journal_title：Biophysical journal

authors： Tsuda Y,Mashimo T,Yoshiya I,Kaseda K,Harada Y,Yanagida T

更新日期：1996-11-01 00:00:00

abstract：:It is shown that alpha-helical content of eleven proteins is well correlated with alanine plus leucine content. These residues, taken singly or together, are to a first approximation randomly distributed in the four proteins whose tertiary structures have been determined (i.e., myoglobin, lysozyme, ribonuclease, alpha...

journal_title：Biophysical journal

authors： Prothero JW

更新日期：1968-11-01 00:00:00

abstract：:The published reports of flash-induced absorbance changes in the 680-690 nm spectral region, which have been attributed to bleaching of the primary reaction center chlorophyll of photosystem II (PSII) P-680, are discussed in light of what is known about the primary electron acceptor of PSII, C-550. The question of whe...

journal_title：Biophysical journal

authors： Butler WL

更新日期：1972-07-01 00:00:00

abstract：:Using both quasi-elastic light scattering spectroscopy and angular dissymmetry in the intensity of the scattered light, we examined the onset of turbidity for intact calf lenses and for isolated nuclear cytoplasm. In the case of the nuclear cytoplasm these measurements demonstrate the presence of two kinds of scattere...

journal_title：Biophysical journal

authors： Delaye M,Clark JI,Benedek GB

更新日期：1982-03-01 00:00:00

abstract：:Cyclic nucleotide-gated (CNG) channels, which were initially studied in retina and olfactory neurons, are activated by cytoplasmic cGMP or cAMP. Detailed comparisons of nucleotide-activated currents using nucleotide analogs and mutagenesis revealed channel-specific residues in the nucleotide-binding domain that regula...

journal_title：Biophysical journal

authors： Scott SP,Cummings J,Joe JC,Tanaka JC

更新日期：2000-05-01 00:00:00