Abstract:
:Changes in absorbance of voltage-sensitive merocyanine-rhodanine dyes were used to monitor electrical responses in the semilunar ganglion of 4-10-day-old developing chick embryos. The electrical responses were simultaneously recorded from many positions in the ganglion. Stimulation of the afferent nerve fibers (the ophthalmic division of cranial nerve V) with a suction electrode led to changes in light absorption of the stained ganglia. With both the depolarizing and hyperpolarizing pulses, the change was largest at 700 nm and was eliminated at a wavelength of 620 nm where the voltage-dependent absorption change of the dyes disappears. In the 4-10-day-old embryonic ganglia, two types of optical membrane potential responses, 'non-conducted' and 'conducted' responses, were identified. The non-conducted response varied with the intensity of the stimulus and had the nature of an electrotonic spread. Furthermore, this non-conducted response exhibited an 'initial upstroke-response' followed by the steady-state plateau evoked by larger depolarizing pulses. The conducted responses were blocked by tetrodotoxin (TTX) or by high external potassium concentration. The incidence of the conducted responses increased as development proceeded from the 5th to the 10th day of age. Thus, the TTX-sensitive action potential activity is probably generated initially in the semilunar ganglion during the 5-10-day-stage of development. These data represent the first demonstration of membrane potential responses in early embryonic intact nervous system. Furthermore, these studies demonstrate the usefulness of voltage-sensitive dyes in the analysis of the organizing process of embryonic neuronal functions during these early stages of development.
journal_name
Brain Resjournal_title
Brain researchauthors
Sakai T,Hirota A,Komuro H,Fujii S,Kamino Kdoi
10.1016/0165-3806(85)90130-0subject
Has Abstractpub_date
1985-01-01 00:00:00pages
39-51issue
1-2eissn
0006-8993issn
1872-6240journal_volume
349pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::We have previously demonstrated resiniferatoxin (RTX) to be an ultrapotent analog of capsaicin. Like capsaicin, RTX initially induces neurogenic inflammation, pain, and hypothermia and then causes desensitization of these responses. We examine here the duration of desensitization following acute treatment with the max...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91705-8
更新日期:1989-11-27 00:00:00
abstract::Hippocampal cholinergic neurostimulating peptide (HCNP), originally isolated from soluble fraction of young rat hippocampus and released from hippocampus by the stimulation of N-methyl-d-aspartate (NMDA) receptors, enhances the cholinergic phenotype development in vitro. HCNP precursor protein (HCNP-pp) has multiple f...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.05.013
更新日期:2007-07-16 00:00:00
abstract::The regional distribution of FLFQPQRFamide binding sites on fresh unfixed cryostate sections from post mortem specimens of human spinal cord and lower medulla oblongata was studied by quantitative autoradiographic methods using [125I]YLFQPQRFamide as ligand. Samples were taken from five cases who had died with no hist...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91531-8
更新日期:1994-01-07 00:00:00
abstract::The short term effects of spinal cord hemisection on protein synthesis in the Cebus monkey were studied by measuring the uptake of [3H]lysine into the trichloroacetic acid (TCA) precipitable and non-precipitable fractions of spinal cord. Animals were sacrificed 3, 6, or 13 days after hemisection. Two normal animals an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)91046-5
更新日期:1976-07-23 00:00:00
abstract::Cyclic AMP is a second messenger by which different extracellular signals are transduced into biological responses. Within the cell, most of the effects of cAMP are mediated through the cAMP protein kinase which appears to be localized in specific compartments of the cell near to their substrate proteins. In the prese...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90244-h
更新日期:1993-01-29 00:00:00
abstract::The effects of bilateral intracranial injections of the D-1 dopamine receptor antagonist SCH 23390 HCl (0, 0.8, 1.6, 3.2, and 6.4 microgram total bilateral dose) administered into the dorsolateral bed nucleus of the stria terminalis (dlBNST) immediately prior to a 3 h intravenous cocaine self-administration session we...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01190-6
更新日期:1998-02-16 00:00:00
abstract::Results from the application of a procedure that allows simultaneous estimation of the rates of glucose transport from blood and of glucose phosphorylation in discrete regions of the brain are given for groups of rats displaying chemically induced motor disturbances. The procedure is based on sequential injections of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90782-4
更新日期:1981-09-28 00:00:00
abstract::The localization of the two Ca-activated extralysosomal proteases m-calpain and mu-calpain in the eye of the adult rabbit was examined by immunohistochemistry, using poly- and monoclonal antibodies against the corresponding rabbit antigens. Immunoreactivity against the two forms of calpains was observed in the epithel...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)90513-m
更新日期:1993-05-21 00:00:00
abstract::Problems in interpretation of autoradiograms generated by ligand binding in brain tissue may be caused by two types of technical limitations: the differential absorption of 3H-generated emissions within the tissue (differential quenching) and the reduced resolution when 125I and 14C are used as isotopes. In the course...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90290-9
更新日期:1988-08-30 00:00:00
abstract::The misfolding and aggregation of the Aβ peptide - a fundamental event in the pathogenesis of Alzheimer׳s disease - can be instigated in the brains of experimental animals by the intracranial infusion of brain extracts that are rich in aggregated Aβ. Recent experiments have found that the peripheral (intraperitoneal) ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.06.047
更新日期:2015-10-05 00:00:00
abstract::The effects of dopamine on glutamate-induced cytotoxicity were examined using the primary cultures of rat striatal neurons. Cell viability was significantly reduced by exposure of cultures to glutamate or kainate for 24 h. In contrast, similar application of N-methyl-D-aspartate (NMDA) or alpha-amino-3-hydroxy-5-methy...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91597-0
更新日期:1994-08-29 00:00:00
abstract::Dorsal temporal pole (dTP) is a cortical region at the rostral end of the superior temporal gyrus that forms part of the ventral auditory object processing pathway. Anatomical connections with frontal and medial temporal areas, as well as a recent single-unit recording study, suggest this area may be an important part...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.12.025
更新日期:2016-06-01 00:00:00
abstract::Oxidative stress and massive production of nitric oxide (NO) have been implicated in the neuropathogenesis following peripheral nerve injury. This study was aimed to ascertain whether melatonin would exert its neuroprotective effect on the lesioned hypoglossal neurons after peripheral axotomy, since it is known to red...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02489-6
更新日期:2000-08-11 00:00:00
abstract::In the present paper immunocytochemical analysis at the fluorescence microscopical level has been performed of neural cell adhesion. molecule (NCAM) immunoreactivity in the adult rat tel- and diencephalon in order to further substantiate the highly selective neuronal localization of NCAM immunoreactivity, using an aff...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(96)01130-4
更新日期:1997-01-23 00:00:00
abstract::In the course of pulse-label studies on the axonal transport of the small, basic, actin-binding proteins--actin depolymerizing factor, cofilin and profilin--in chicken motor neurones, we observed a heavily labelled protein of M(r) 18 kDa and pI 8.2 on fluorographs of two-dimensional polyacrylamide gels. On the basis o...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00766-x
更新日期:1997-10-17 00:00:00
abstract::A combined retrograde transport-immunohistochemical method was used to compare the numbers of corticotropin-releasing factor (CRF) and vasopressin-immunoreactive neurons in the paraventricular nucleus of the hypothalamus (PVH) that could be retrogradely labeled after tracer injections in the dorsomedial medulla or the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91641-6
更新日期:1987-12-29 00:00:00
abstract::Hypophysiotropic thyrotropin-releasing hormone (TRH)-synthesizing neurons, the central regulators of the hypothalamic-pituitary-thyroid axis, are located in the paraventricular nucleus of the hypothalamus (PVN). These neurons are well-known to be stimulated by cold exposure through activation of ascending brainstem pa...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.07.094
更新日期:2009-10-19 00:00:00
abstract::Adequate grip force modulation is critical to manual dexterity and often impaired in hemiparetic stroke patients. Previous studies in hemiparetic patients suggest that aspects of grip force control may be differently affected by the lesion. We developed a visuomotor power grip force-tracking task allowing quantificati...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2012.03.007
更新日期:2012-05-03 00:00:00
abstract::The rat hippocampus contains an acidic macromolecular neurotrophic factor (NTF) which supports the survival of cultured chick ciliary ganglion cells. Hippocampal NTF increases approximately 2-fold from birth to adulthood with no further change with aging. Disruption of extrinsic inputs to the hippocampus from the ento...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(86)91015-2
更新日期:1986-01-22 00:00:00
abstract::To investigate the effects of ionic changes possibly associated with hibernation, hippocampal slices prepared from golden hamsters were studied in artificial cerebrospinal fluid (ACSF) of variable composition (K+ 3-5 mM, Ca2+ 2-4 mM, Mg2+ 2-4 mM, pH 7.0-7.7) at temperatures of 15-20 degrees C, just above the temperatu...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00524-t
更新日期:1995-08-14 00:00:00
abstract::The optic tecta of 55 Rutilus rutilus were examined at intervals varying from 2 days to 4 years after unilateral retinal ablation. Qualitative ultrastructural examination of the retinal target lamina of the optic tectum (stratum fibrosum et griseum superficiale, SFGS) revealed that an initial period of degeneration an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91133-x
更新日期:1988-05-03 00:00:00
abstract::The two primary categories of stroke, ischemic and hemorrhagic, both have fundamentally different mechanisms and thus different treatment options. These two stroke categories were applied to rat models to identify potential biomarkers that can distinguish between them. Ischemic stroke was induced by middle cerebral ar...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2013.09.051
更新日期:2013-12-02 00:00:00
abstract::Cerebrospinal fluid (c.s.f.) pressure and resistance to absorption have been studied in exteriorized fetuses (18 to 21 days gestation) from pregnant rats anaesthetised with pentobarbitone and in similarly anaesthetised postnatal rats at 1, 5, 10 and 30 days after birth and in adults. The resting c.s.f. pressure was lo...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(87)90172-6
更新日期:1987-05-01 00:00:00
abstract::The melanocortin (MC)4 receptor is important for food intake and weight homeostasis as it mediates the orexigenic and anorexigenic effects of the MCs. OLETF (Otsuka-Long-Evans-Tokushima-Fatty) rats are a selective inbred strain of polygenic variant rats which overeat and develop obesity with elevated leptin levels. We...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02125-3
更新日期:2000-01-03 00:00:00
abstract::Safe, long-term gene expression is a primary criteria for effective gene therapy in the brain, so studies were initiated to evaluate adeno-associated virus (AAV) vector transfer of a reporter gene into specific sites of the rat brain. In the 4 day old rat, site infusions of AAV-CMV-lacZ (1 microliter; 5 x 10(4) partic...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01488-8
更新日期:1996-03-25 00:00:00
abstract::Motor cortex stimulation (MCS) is a promising clinical procedure to help alleviate chronic pain. Animal models demonstrated that MCS is effective in lessening nocifensive behaviors. The present study explored the effects of MCS on cortical somatosensory evoked potentials (SEPs) recorded at the primary somatosensory co...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2012.04.027
更新日期:2012-06-29 00:00:00
abstract::We have previously demonstrated that NMDA receptor-mediated extracellular adenosine accumulation in neuronal cultures is receptor-mediated and requires calcium influx. Because protein kinase C (PKC) is a calcium-dependent enzyme, we hypothesized that activation of PKC might be involved in NMDA-mediated adenosine accum...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.04.057
更新日期:2007-06-25 00:00:00
abstract::Projection neurons from the superior colliculus (SC) to the cuneiform region (CFR) of the midbrain form distinct cell clusters in the intermediate gray layer which precisely overlap the cholinergic fiber patches in this region of the cat SC (Jeon and Mize, J. Comp. Neurol., 337 (1993) 127-150). The acetylcholine (ACh)...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00563-x
更新日期:1996-09-16 00:00:00
abstract::The aim of this study was to investigate the degree of functional and anatomical overlap between two major neuronal subpopulations in the rostral ventrolateral medulla: pre-sympathetic (sympathoexcitatory) neurons, and expiratory neurons of the Bötzinger complex. Extracellular recordings were made with dye-filled micr...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)00814-7
更新日期:1995-11-13 00:00:00
abstract::The cholinergic agonist pilocarpine injected intraperitoneally (ip) increases mean arterial pressure (MAP) and superior mesenteric (SM) vascular resistance and reduces submandibular/sublingual gland (SSG) vascular resistance. In the present study, we investigated the effects of electrolytic lesions of the anteroventra...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.06.071
更新日期:2005-09-07 00:00:00