Fluorescein angiography basic science and engineering.

Abstract:

:Fluorescein angiography is an application of the physical phenomenon of fluorescence, which is phosphorescence in which the quantum mechanical decay curve is so rapid that it appears instantaneous, and it consequently has no afterglow. Sodium fluorescein is excited by light energy between 465 and 490 nm, and it decays into a lower state emitting light energy between 520 and 530 nm as fluorescent radiation. The free electrons available for excitation are reduced by chemical bonding between the fluorescein dye and plasma proteins to which up to 80% of the dye is bound in the bloodstream, thus reducing overall fluorescence. Optimalization of the observed and recorded fluorescence is afforded by providing exciter and barrier filters with as little overlap as possible to reduce or eliminate contrast reducing pseudofluorescence.

journal_name

Ophthalmology

journal_title

Ophthalmology

authors

Wolfe DR

doi

10.1016/s0161-6420(86)33521-8

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

1617-20

issue

12

eissn

0161-6420

issn

1549-4713

pii

S0161-6420(86)33521-8

journal_volume

93

pub_type

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