Isolation and identification of mutagenic polycyclic aromatic hydrocarbons from a coal gasifier condensate.

Abstract:

:An iterative scheme of short-term bacterial mutagenesis assays and multidimensional liquid chromatographic methods was used to isolate mutagens from a neutral fraction of a complex carcinogenic tar formed as a by-product of coal gasification. Mutagens in the complementary reversed- and normal-phase liquid chromatographic fractions were identified by capillary column gas chromatography and gas chromatography-mass spectrometry. The principal mutagens included a variety of 4- to 6-ring unsubstituted polycyclic aromatic hydrocarbons, their alkylated homologs, and related methylene-bridged compounds. Most of the unsubstituted mutagens were known carcinogens that were qualitatively similar to those detected by carcinogenicity assays of fractions from other types of coal tars and cigarette smoke condensate. The chromatographic approach is generally applicable to complex mixtures and is especially well suited for fractionating mixtures of aromatic compounds and their alkyl homologs.

journal_name

Environ Res

journal_title

Environmental research

authors

Haugen DA,Stamoudis VC

doi

10.1016/s0013-9351(86)80135-9

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

400-19

issue

2

eissn

0013-9351

issn

1096-0953

pii

S0013-9351(86)80135-9

journal_volume

41

pub_type

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