Abstract:
:In the mammalian pineal gland, the rhythm in melatonin biosynthesis depends on the norepinephrine (NE)-driven regulation of arylalkylamine N-acetyltransferase (AANAT), the penultimate enzyme of melatonin biosynthesis. A recent study showed that phytocannabinoids like tetrahydrocannabinol reduce AANAT activity and attenuate NE-induced melatonin biosynthesis in rat pineal glands, raising the possibility that an endocannabinoid system is present in the pineal gland. To test this hypothesis, we analyzed cannabinoid (CB) receptors and specific enzymes for endocannabinoid biosynthesis or catabolism in rat pineal glands and cultured pinealocytes. Immunohistochemical and immunoblot analyses revealed the presence of CB1 and CB2 receptor proteins, of N-acyl phosphatidyl ethanolamine hydrolyzing phospholipase D (NAPE-PLD), an enzyme catalyzing endocannabinoid biosynthesis and of fatty acid amide hydrolase (FAAH), an endocannabinoid catabolizing enzyme, in pinealocytes, and in pineal sympathetic nerve fibers identified by double immunofluorescence with an antibody against tyrosine hydroxylase. The immunosignals for the CB2 receptor, NAPE-PLD, and FAAH found in pinealocytes did not vary under a 12 hr light:12 hr dark cycle. The CB1 receptor immunoreaction in pinealocytes was significantly reduced at the end of the light phase [zeitgeber time (ZT) 12]. The immunosignal for NAPE-PLD found in pineal sympathetic nerve fibers was reduced in the middle of the dark phase (ZT 18). Stimulation of cultured pinealocytes with NE affected neither the subcellular distribution nor the intensity of the immunosignals for the investigated CB receptors and enzymes. In summary, the pineal gland comprises indispensable compounds of the endocannabinoid system indicating that endocannabinoids may be involved in the control of pineal physiology.
journal_name
J Pineal Resjournal_title
Journal of pineal researchauthors
Koch M,Habazettl I,Dehghani F,Korf HWdoi
10.1111/j.1600-079X.2008.00597.xsubject
Has Abstractpub_date
2008-11-01 00:00:00pages
351-60issue
4eissn
0742-3098issn
1600-079Xpii
JPI597journal_volume
45pub_type
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1998.tb00545.x
更新日期:1998-09-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1988.tb00782.x
更新日期:1988-01-01 00:00:00
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journal_title:Journal of pineal research
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doi:10.1111/j.1600-079x.1998.tb00390.x
更新日期:1998-12-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12018
更新日期:2013-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2015-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12636
更新日期:2020-04-01 00:00:00
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journal_title:Journal of pineal research
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更新日期:2015-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1995.tb00148.x
更新日期:1995-03-01 00:00:00
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更新日期:2020-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.2007.00536.x
更新日期:2008-05-01 00:00:00
abstract::Melatonin, which is contained in certain vegetables, may have an influence on circulatory melatonin concentrations. This study examined the effects of the consumption of vegetables on 6-sulfatoxymelatonin concentrations in morning urine. Ninety-four healthy women aged 24-55 were recruited through a city public health ...
journal_title:Journal of pineal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1600-079X.2007.00549.x
更新日期:2008-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1999.tb00566.x
更新日期:1999-01-01 00:00:00
abstract::In this study, the effect of melatonin on sodium arsenite (arsenite)-induced peripheral neurotoxicity was investigated using dorsal root ganglion (DRG) explants. After 24-hr incubation, arsenite (30 microm) consistently elevated the expression of heat shock protein 70 and haeme oxygenase-1, two well-known stress prote...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2008.00629.x
更新日期:2009-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1991.tb00826.x
更新日期:1991-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章,随机对照试验
doi:10.1111/jpi.12138
更新日期:2014-08-01 00:00:00
abstract::Diurnal variations in serum melatonin levels and pineal concentrations of serotonin (5-HT), N-acetylserotonin (NAS), 5-hydroxyindole acetic acid (5-HIAA), and melatonin were estimated in adult male Djungarian hamsters kept under long-day (LD 16:8) or short-day (LD 8:16) photoperiods. The nocturnal increase in melatoni...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1992.tb00039.x
更新日期:1992-04-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1996.tb00266.x
更新日期:1996-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
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journal_title:Journal of pineal research
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更新日期:1990-01-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.2010.00755.x
更新日期:2010-05-01 00:00:00
abstract::The milk-ejecting response of lactating mouse mammary gland tissue to ovine pineal extracts indicated the presence of a neurohormone-like bioactivity in this tissue. After successive fractionation on gel permeation chromatography and reversed-phase liquid chromatography (HPLC) in conjunction with radioimmunoassays (RI...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1988.tb00800.x
更新日期:1988-01-01 00:00:00
abstract::The influence of the pineal gland on the histopathologic changes induced in mammary glands by 7, 12-dimethylbenz(a)-anthracene (DMBA) has been studied. To achieve the maximum expression of pineal action in animals, 28-day-old female Sprague-Dawley rats were subjected to one of the following treatments: blindness + olf...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1989.tb00418.x
更新日期:1989-01-01 00:00:00
abstract::A role of D2-dopaminergic neurotransmission in the regulation of melatonin biosynthesis in retina was studied in vivo in chickens. The nighttime rise in serotonin N-acetyltransferase (NAT)--the penultimate and key regulatory melatonin-synthesizing enzyme--was potently inhibited by both acute light exposure and agonist...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079x.1991.tb00466.x
更新日期:1991-10-01 00:00:00
abstract::The protective role of exogenous melatonin on U-induced nephrotoxicity was investigated in rats. Animals were given single doses of uranyl acetate dihydrate (UAD) at 5 mg/kg (subcutaneous), melatonin at 10 or 20 mg/kg (intraperitoneal), and UAD (5 mg/kg) plus melatonin (10 or 20 mg/kg), or vehicle (control group). In ...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00447.x
更新日期:2007-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12652
更新日期:2020-08-01 00:00:00
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journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00520.x
更新日期:2008-04-01 00:00:00
abstract::Melatonin has demonstrated a potential protective effect in central nervous system. Thus, it is interesting to determine whether pre-ischemia melatonin administration could protect against cerebral ischemia/reperfusion (IR)-related injury and the underlying molecular mechanisms. In this study, we revealed that IR inju...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/jpi.12395
更新日期:2017-04-01 00:00:00
abstract::This study examined the factors that may be involved in altering the function of pineal gland in hypoxic conditions. Adult Wistar rats were subjected to hypoxia and the pineal gland was examined for the mRNA and protein expression of hypoxia-inducible factor-1alpha (HIF-1alpha), vascular endothelial growth factor (VEG...
journal_title:Journal of pineal research
pub_type: 杂志文章
doi:10.1111/j.1600-079X.2007.00424.x
更新日期:2007-04-01 00:00:00