The binding of auxin to the Arabidopsis auxin influx transporter AUX1.

Abstract:

:The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood, and we sought to address this in the present investigation. We expressed AUX1 to high levels in a baculovirus expression system and prepared membrane fragments from baculovirus-infected insect cells. These membranes proved suitable for determination of the binding of IAA to AUX1 and enabled us to determine a K(d) of 2.6 mum, comparable with estimates for the K(m) for IAA transport. The efficacy of a number of auxin analogues and auxin transport inhibitors to displace IAA binding from AUX1 has also been determined and can be rationalized in terms of their physiological effects. Determination of the parameters describing the initial interaction between a plant transporter and its hormone ligand provides novel quantitative data for modeling auxin fluxes.

journal_name

Plant Physiol

journal_title

Plant physiology

authors

Carrier DJ,Bakar NT,Swarup R,Callaghan R,Napier RM,Bennett MJ,Kerr ID

doi

10.1104/pp.108.122044

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

529-35

issue

1

eissn

0032-0889

issn

1532-2548

pii

pp.108.122044

journal_volume

148

pub_type

杂志文章
  • Flavone synthases from Medicago truncatula are flavanone-2-hydroxylases and are important for nodulation.

    abstract::Flavones are important copigments found in the flowers of many higher plants and play a variety of roles in plant adaptation to stress. In Medicago species, flavones also act as signal molecules during symbiotic interaction with the diazotropic bacterium Sinorhizobium meliloti. They are the most potent nod gene induce...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.106.095018

    authors: Zhang J,Subramanian S,Zhang Y,Yu O

    更新日期:2007-06-01 00:00:00

  • Overexpression of the RieskeFeS Protein Increases Electron Transport Rates and Biomass Yield.

    abstract::In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochrome b6f (cyt b6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cyt f (PetA) and cyt b6 (PetB), core proteins of t...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.17.00622

    authors: Simkin AJ,McAusland L,Lawson T,Raines CA

    更新日期:2017-09-01 00:00:00

  • Characterization of a molecular modification of phytochrome that is associated with its conversion to the far-red-absorbing form.

    abstract::Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior when compared to soluble phytochrome isolated from shoots that had never been irradiated. This modified behavior includes ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.68.4.789

    authors: Boeshore ML,Pratt LH

    更新日期:1981-10-01 00:00:00

  • Developmental history affects the susceptibility of spinach leaves to in vivo low temperature photoinhibition.

    abstract::Room temperature chlorophyll a fluorescence was used to determine the effects of developmental history, developmental stage, and leaf age on susceptibility of spinach to in vivo low temperature (5 degrees C) induced photoinhibition. Spinach (Spinacia oleracea cv Savoy) leaves expanded at cold hardening temperatures (5...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.99.3.1141

    authors: Boese SR,Huner NP

    更新日期:1992-07-01 00:00:00

  • Effects of temperature, water potential, and light on germination responses of redroot pigweed seeds to ethylene.

    abstract::The responses of redroot pigweed (Amaranthus retroflexus L.) seeds to nine ethylene concentrations between 0.5 and 50 microliters per liter were assessed at different temperatures and water potentials and in either continuous white light or darkness. Under all experimental treatments, the probit-transformed percentage...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.6.1149

    authors: Schonbeck MW,Egley GH

    更新日期:1980-06-01 00:00:00

  • The PsENOD12 Gene Is Expressed at Two Different Sites in Afghanistan Pea Pseudonodules Induced by Auxin Transport Inhibitors.

    abstract::A number of early nodulin genes are expressed in specific cell types as pea (Pisum sativum) root nodules develop. The Pisum sativum early nodulin PsENOD2 is detected only in the uninfected cells of the nodule parenchyma, whereas PsENOD12 is expressed at two spatially removed sites: in root hairs and adjacent cortical ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.100.4.1649

    authors: Scheres B,McKhann HI,Zalensky A,Löbler M,Bisseling T,Hirsch AM

    更新日期:1992-12-01 00:00:00

  • Synthesis of abscisic Acid-responsive, heat-stable proteins in embryonic axes of dormant wheat grain.

    abstract::Germination of embryonic axes from dormant grain is inhibited by low concentrations of abscisic acid (ABA) compared with axes from nondormant grain. Incubation of dormant grain axes in 0.05 to 50 micromolar ABA caused the prolonged synthesis of a set of heat-stable proteins. Two of these proteins were identified as de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.93.2.662

    authors: Ried JL,Walker-Simmons MK

    更新日期:1990-06-01 00:00:00

  • Effect of substrate salinity on the ability for protein synthesis in pea roots.

    abstract::THE EFFECT OF SALINITY ON INCORPORATION OF AMINO ACIDS INTO ROOT TIP PROTEIN IS APPARENTLY OF DUAL NATURE: in presence of salts the uptake is depressed and the normal metabolic pathways are disturbed. If the roots were grown at high salt concentration, uptake and incorporation are affected even if they are carried out...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.43.7.1115

    authors: Kahane I,Poljakoff-Mayber A

    更新日期:1968-07-01 00:00:00

  • myo-Inositol 1-Phosphate Synthase Inhibition and Control of Uridine Diphosphate-d-glucuronic Acid Biosynthesis in Plants.

    abstract::Of the eight intermediates associated with the two pathways of UDP-d-glucuronic acid biosynthesis found in plants, only d-glucuronic acid inhibited myo-inositol 1-phosphate synthase (EC 5.5.1.4), formerly referred to as d-glucose 6-phosphate cycloaldolase. Inhibition was competitive. An attempt to demonstrate over-all...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.3.368

    authors: Loewus MW,Loewus F

    更新日期:1974-09-01 00:00:00

  • Intracellular Localization of the Neurotoxin 2,4-Diaminobutyric Acid in Lathyrus sylvestris L. Leaf Tissue.

    abstract::The intracellular distribution of the neurotoxin 2,4-diaminobutyric acid (DABA) in mature leaves of the perennial legume Lathyrus sylvestris L. var ;Lathco' (flatpea) was determined using subcellular fractions from mesophyll protoplasts. Chloroplasts contained about 15% of the cellular DABA. At least 75% of the DABA w...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.4.900

    authors: Foster JG,Cress WD,Wright SF,Hess JL

    更新日期:1987-04-01 00:00:00

  • Adaptations of Photosynthetic Electron Transport, Carbon Assimilation, and Carbon Partitioning in Transgenic Nicotiana plumbaginifolia Plants to Changes in Nitrate Reductase Activity.

    abstract::Transgenic Nicotiana plumbaginifolia plants that express either a 5-fold increase or a 20-fold decrease in nitrate reductase (NR) activity were used to study the relationships between carbon and nitrogen metabolism in leaves. Under saturating irradiance the maximum rate of photosynthesis, per unit surface area, was de...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.104.1.171

    authors: Foyer CH,Lescure JC,Lefebvre C,Morot-Gaudry JF,Vincentz M,Vaucheret H

    更新日期:1994-01-01 00:00:00

  • Characterization of cadmium uptake by plant tissue.

    abstract::The uptake of cadmium by excised root tissue of barley (Hordeum vulgare L. cv. Arivat) was investigated with respect to kinetics, concentration, and interactions with various cations. The role of metabolism in Cd absorption was examined using a range of temperatures, anaerobic treatments, and chemical inhibitors. The ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.54.1.67

    authors: Cutler JM,Rains DW

    更新日期:1974-07-01 00:00:00

  • Quantitation of Chill-Induced Release of a Tubulin-Like Factor and Its Prevention by Abscisic Acid in Gossypium hirsutum L.

    abstract::The degree of tubulin polymerization in cotton (Gossypium hirsutum L. cv Acala) cotyledonary tissue was estimated by radioimmunoassay which measured the amount of a tubulin-like factor. It was assumed that the release of this tubulin-like factor indicated depolymerization of microtubules. Exposure to chilling resulted...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.71.4.747

    authors: Rikin A,Atsmon D,Gitler C

    更新日期:1983-04-01 00:00:00

  • Light and the correlation of chloroplast development and coupling of phosphorylation to electron transport.

    abstract::Coupling of phosphorylation to electron transport was examined by measuring the photosynthetic control ratio for broken wheat plastids isolated from seedlings at different greening stages. The photosynthetic control ratio progressively increased during greening and tight coupling was noted after granal stacking and th...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.65.5.880

    authors: Duysen ME,Freeman TP,Zabrocki RD

    更新日期:1980-05-01 00:00:00

  • Hormonal regulation of cell elongation in the hypocotyl of rootless soybean: an evaluation of the role of DNA synthesis.

    abstract::A method was developed where soybean seedlings were grown without roots to study the influence of hormones of root origin on shoot growth. Excision of the root resulted in inhibition of apical section growth and DNA synthesis and inhibited elongating section growth. A synthetic cytokinin restored DNA synthesis in the ...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.44.9.1295

    authors: Holm RE,Key JL

    更新日期:1969-09-01 00:00:00

  • Photosynthetic carbon metabolism in isolated maize bundle sheath strands.

    abstract::Photosynthetically active bundle sheath strands capable of assimilating up to 8 micromoles CO(2) per milligram chlorophyll per hour have been isolated from fully expanded leaves of Zea mays L. Mesophyll cell contamination of the preparations was negligible, as evidenced by light and electron microscopy and by a high r...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.51.4.787

    authors: Chollet R

    更新日期:1973-04-01 00:00:00

  • Architecture-based multiscale computational modeling of plant cell wall mechanics to examine the hydrogen-bonding hypothesis of the cell wall network structure model.

    abstract::A primary plant cell wall network was computationally modeled using the finite element approach to study the hypothesis of hemicellulose (HC) tethering with the cellulose microfibrils (CMFs) as one of the major load-bearing mechanisms of the growing cell wall. A computational primary cell wall network fragment (10 × 1...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.112.201228

    authors: Yi H,Puri VM

    更新日期:2012-11-01 00:00:00

  • Auxin receptors of maize coleoptile membranes do not have ATPase activity.

    abstract::Membrane-localized auxin-binding sites from coleoptiles and primary leaves of Zea mays L. which may be auxin receptors can be fully solubilized by 1 to 1.5 mg of Triton X-100 per mg of membrane protein (about 1 mg per gram of original tissue fresh weight), while 70% of the basal (Mg(2+))-ATPase and 85% of the K(+)-sti...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.61.4.581

    authors: Cross JW,Briggs WR

    更新日期:1978-04-01 00:00:00

  • Translational regulation of the synthesis of euglena fumarase by light and ethanol.

    abstract::Ethanol addition or the exposure of dark-grown resting Euglena to light produced a transient increase in the rate of fumarase synthesis as measured by pulse-labeling with [(35)S]sulfate and immunoprecipitation. The half-life of fumarase in the dark and in the light was similar. In the plastidless mutant W(3)BUL, fumar...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.1.63

    authors: Rikin A,Schwartzbach SD

    更新日期:1989-05-01 00:00:00

  • A β-Ketoacyl-CoA Synthase Is Involved in Rice Leaf Cuticular Wax Synthesis and Requires a CER2-LIKE Protein as a Cofactor.

    abstract::Cuticular waxes are complex mixtures of very-long-chain fatty acids (VLCFAs) and their derivatives, forming a natural barrier on aerial surfaces of terrestrial plants against biotic and abiotic stresses. In VLCFA biosynthesis, β-ketoacyl-CoA synthase (KCS) is the key enzyme, catalyzing the first reaction in fatty acid...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.16.01527

    authors: Wang X,Guan Y,Zhang D,Dong X,Tian L,Qu LQ

    更新日期:2017-02-01 00:00:00

  • Putrescine-induced wounding and its effects on membrane integrity and ion transport processes in roots of intact corn seedlings.

    abstract::Interactions between putrescine and membrane function were examined with the use of a recently developed microelectrode system that enables us simultaneously to quantify membrane potentials and net K(+) fluxes associated with individual cells at the root surface of an intact corn (Zea mays L.) seedling. In contrast to...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.90.3.988

    authors: Ditomaso JM,Shaff JE,Kochian LV

    更新日期:1989-07-01 00:00:00

  • alpha-L-fucosyltransferases from radish primary roots.

    abstract::A novel alpha-L-fucosyltransferase capable of transferring L-fucose (L-Fuc) from GDP-L-Fuc to the O-2 of alpha-L-arabinofuranosyl residue (GDP-L-Fuc:alpha-L-arabinofuranoside 2-alpha-L-fucosyltransferase) has been found in the microsomal fraction of primary roots from 6-d-old radish (Raphanus sativus L.) seedlings. En...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.110.2.665

    authors: Misawa H,Tsumuraya Y,Kaneko Y,Hashimoto Y

    更新日期:1996-02-01 00:00:00

  • Polygalacturonase Isozymes and Pectin Depolymerization in Transgenic rin Tomato Fruit.

    abstract::We have previously described the construction and expression of a chimeric gene that allows developmentally regulated expression of tomato (Lycopersicon esculentum) polygalacturonase in ripening-impaired, mutant (rin) tomato fruit (JJ Giovannoni, D DellaPenna, AB Bennett, RL Fischer [1989] The Plant Cell 1: 53-63). We...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.94.4.1882

    authors: Dellapenna D,Lashbrook CC,Toenjes K,Giovannoni JJ,Fischer RL,Bennett AB

    更新日期:1990-12-01 00:00:00

  • Some aspects of the control of root growth and georeaction: the involvement of indoleacetic Acid and abscisic Acid.

    abstract::Apical segments of roots of Zea mays L. cv. Orla and cv. Anjou show a strong georeaction during 7 hours geostimulation. This is abolished by detipping the segments and restored by replacing the tips upon the apical cut surfaces. After exodiffusion of endogenous indoleacetic acid (IAA) the retipped segments showed a si...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.67.5.1047

    authors: Pilet PE

    更新日期:1981-05-01 00:00:00

  • Effect of a Brief CO(2) Exposure on Ethylene Production.

    abstract::Ethylene production and respiration by Granny Smith apples were inhibited by treatment with 20% CO(2) for 2 hours. A similar effect was observed in tissue slices when treated at either 0 or 25 degrees C.The inhibition continued even after an extended aeration period. There is also an inhibition of ethylene emission in...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.76.1.88

    authors: Chaves AR,Tomás JO

    更新日期:1984-09-01 00:00:00

  • Effect of head removal on leaf senescence of sunflower.

    abstract::Greenhouse and field studies examined the effect of flower or seedhead removal on leaf senescence and associated changes in sunflower (Helianthus annuus L.) plants. At intervals during seed development, selected leaves (leaves 6 through 8 from the top in the greenhouse and leaf 7 from the top in the field) were harves...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.83.4.844

    authors: Ho I,Below FE,Hageman RH

    更新日期:1987-04-01 00:00:00

  • Control of specific gene expression by gibberellin and brassinosteroid.

    abstract::We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:

    authors: Bouquin T,Meier C,Foster R,Nielsen ME,Mundy J

    更新日期:2001-10-01 00:00:00

  • Enhanced Disease Susceptibility1 Mediates Pathogen Resistance and Virulence Function of a Bacterial Effector in Soybean.

    abstract::Enhanced disease susceptibility1 (EDS1) and phytoalexin deficient4 (PAD4) are well-known regulators of both basal and resistance (R) protein-mediated plant defense. We identified two EDS1-like (GmEDS1a/GmEDS1b) proteins and one PAD4-like (GmPAD4) protein that are required for resistance signaling in soybean (Glycine m...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.114.242495

    authors: Wang J,Shine MB,Gao QM,Navarre D,Jiang W,Liu C,Chen Q,Hu G,Kachroo A

    更新日期:2014-07-01 00:00:00

  • Solute distribution in sugar beet leaves in relation to Phloem loading and translocation.

    abstract::The distribution of solutes in the various cells of sugar beet (Beta vulgaris L.) source leaves, petioles, and sink leaves was studied in tissue prepared by freeze-substitution. The differences in degree of cryoprotection indicated that sieve elements and companion cells of the source leaf, petiole, and sink leaf cont...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.52.6.585

    authors: Geiger DR,Giaquinta RT,Sovonick SA,Fellows RJ

    更新日期:1973-12-01 00:00:00

  • Synthesis and apparent turnover of Acid invertase in relation to invertase inhibitor in wounded sweet potato root tissue.

    abstract::Previously we showed that acid invertase activity increased and then decreased rapidly in wounded sweet potato (Ipomoea batatas Liam.) root tissue, and that the tissue contained a heat-stable, proteinaceous inhibitor with a molecular weight of about 19,500 daltons.In response to wounding of sweet potato root tissue, i...

    journal_title:Plant physiology

    pub_type: 杂志文章

    doi:10.1104/pp.59.5.879

    authors: Matsushita K,Uritani I

    更新日期:1977-05-01 00:00:00