Abstract:
:The cellular import of the hormone auxin is a fundamental requirement for the generation of auxin gradients that control a multitude of plant developmental processes. The AUX/LAX family of auxin importers, exemplified by AUX1 from Arabidopsis (Arabidopsis thaliana), has been shown to mediate auxin import when expressed heterologously. The quantitative nature of the interaction between AUX1 and its transport substrate indole-3-acetic acid (IAA) is incompletely understood, and we sought to address this in the present investigation. We expressed AUX1 to high levels in a baculovirus expression system and prepared membrane fragments from baculovirus-infected insect cells. These membranes proved suitable for determination of the binding of IAA to AUX1 and enabled us to determine a K(d) of 2.6 mum, comparable with estimates for the K(m) for IAA transport. The efficacy of a number of auxin analogues and auxin transport inhibitors to displace IAA binding from AUX1 has also been determined and can be rationalized in terms of their physiological effects. Determination of the parameters describing the initial interaction between a plant transporter and its hormone ligand provides novel quantitative data for modeling auxin fluxes.
journal_name
Plant Physioljournal_title
Plant physiologyauthors
Carrier DJ,Bakar NT,Swarup R,Callaghan R,Napier RM,Bennett MJ,Kerr IDdoi
10.1104/pp.108.122044subject
Has Abstractpub_date
2008-09-01 00:00:00pages
529-35issue
1eissn
0032-0889issn
1532-2548pii
pp.108.122044journal_volume
148pub_type
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