Abstract：
:Explantation and cultivation in vitro of 55 human biopsies of histologically normal urothelial tissue, and 63 biopsies from transitional cell carcinomas (TCC) revealed a primary success rate of about 75% and a secondary success rate of about 50% with no significant differences, between normal and TCC derived cultures. While only half of the subcultured biopsies of normal origin were able to grow for more than 4 passages all TCC derived cultures were able to do so. Based on morphology, life span, invasive growth into co-cultured fragments of embryonic chick heart, and tumorigenicity in nude mice the following transformation grades (TGr) were defined: Normal (TGr 0), non-malignant with prolonged, but finite life span (TGr I), premalignant with infinite life span but without tumorigenic and invasive potential (TGr II), malignant with infinite life span and tumorigenic and invasive properties (TGr III). Differentiation between TGr I - TGr III was possible by several other parameters, including cytological characteristics, molecular and immunological surface structure, relative oncogene expression, pyruvate kinase isoenzymes, DNA Repair, and growth pattern. However, in addition to these common characteristics, the individual cell lines of the various categories of transformation showed differences that allowed the identification of well defined cell lines and sublines.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Kieler J,Christensen B,Ottesen S,Tromholt V,Ostrowski Ksubject
Has Abstractpub_date
1987-09-01 00:00:00pages
959-69issue
5Beissn
0250-7005issn
1791-7530journal_volume
7pub_type
杂志文章abstract：:The relationship between production of Colony-Stimulating Factor (CSF) and metastasis has been investigated in the TS/A murine model. CSF production was determined in TS/A cell variants isolated through serial in vivo selection of lung metastatic nodules induced by intravenous or subcutaneous injection of tumor cells ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:For the determination of LDL receptor expression on living human cells two monoclonal antibodies specific for the extracellular domain of LDL receptor were established using affinity-purified LDL receptor and carrier-conjugated LDL receptor peptide 163-174 as immunizing antigens. The 125I-labeled antibodies were used ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Melanoma cells express the nuclear vitamin D receptor (VDR), indicating that malignant melanoma represents a promising target for treatment with 1,25-dihydroxyvitamin D3 (1,25(OH)(2)D(3)) or its analogs. We previously showed that some melanoma cell lines are resistant to the antiproliferative effects of 1,25...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND/AIM:We evaluated the clinical impact of FOLFOXIRI regimen aiming for conversion surgery in patients with unresectable multiple colorectal liver metastasis (CRLM). PATIENTS AND METHODS:A total of 42 patients with unresectable multiple CRLM who received chemotherapy with molecular agents were included in the ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13703
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND/AIM:Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an anti-hypoglycemic drug that appears to have anticancer effects. However, little is known about the effect of metformin on pNETs. In this study, we investigated the anti-p...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13933
更新日期：2020-01-01 00:00:00
abstract：:CEA is discussed as a potential tumour marker for carcinomas of the urothelium. However, investigations by means of polyclonal antibodies have shown various cross reactions which are not to be expected with monoclonal antibodies (MAb). Immunohistochemistry was done with the MAb anti-CEA BW 431/26 (Behring), N 1522 (Da...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Three monoclonal antiidiotypic antibodies (AIA) to MOPC 315 IgA, G3 (IgG2b), A2 (IgG1) and D10 (a hybrid molecule consisting of gamma 1 and gamma 2a heavy chains), were characterized with respect to their binding constants (Ka) to MOPC 315 mouse myeloma cells. The Ka of G3 and A2 was 10(8)/mole; and that of D10 was 3 ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Curcumin's instability and its metabolite, tetrahydrocurcumin (THC) pose a major issue for the establishment of dependable pharmacokinetics and excretion profiles. Additional pharmacokinetic variances are associated with durations of intravenous infusions. We found that stabilizing curcumin with phosphoric acid allows...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:Vulvar cancer is a rare malignancy, representing approximately 5% of all female genital tract cancers and 1% of all malignancies in women. The incidence of vulvar carcinoma in situ (VIN III) has nearly doubled from 1.1 to 2.1 per 100,000 women-years between 1973 and 1987. It is more frequent among older women...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:Adjuvant radioimmunotherapy (RIT) for human colonic cancer was performed in a nude rat model of experimental liver metastases. Thirty-three rats were injected intraportally through a mesenteric vein with 5 x 10(6) cells from the human colonic cancer cell line LS174T. Within half an hour, 20 MBq (n = 2), 75 MBq (n = 5)...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:Prostatic 25-hydroxyvitamin D-1alpha-hydroxylase (1alpha-OHase) is up-regulated by epidermal growth factor (EGF) and down-regulated by 1alpha,25-dihydroxyvitamin D [1alpha,25(OH)2D] at the promoter level in an autocrine/paracrine fashion, suggesting that local production of 1alpha,25(OH)2D could provide an important c...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND/AIM:Metastases involving the adrenal glands can be treated surgically or with radiation therapy. Retrospective studies indicate that radiotherapy for this indication is safe, well-tolerated and associated with symptom palliation and good local control. We conducted this analysis to report on patterns and out...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12788
更新日期：2018-08-01 00:00:00
abstract：:Two monoclonal antibodies, KM227 (IgM) and KM432 (IgG1), were established by immunizing mice with the crude membrane fraction of lung squamous cell carcinoma and the high-molecular-weight antigen in pleural perfusion from patients with lung adenocarcinoma, respectively. They were found to recognize the carbohydrate ep...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1993-05-01 00:00:00
abstract：:1-(phenylmethyl)-4,7,10-tris-[(4'methylphenyl)-sulfonyl]-1,4,7, 10-tetraazacyclododecane proved to possess potent in vivo antineoplastic and in vitro cytotoxicity in murine and human tissue cultured cells. In Tmolt3 leukemic cells 1-(phenylmethyl)-4,7,10-tris-[(4'methylphenyl)sulfonyl]-1,4,7, 10-tetraazacyclododecane ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-03-01 00:00:00
abstract：:The p53 gene product suppresses both tumor cell growth and the cellular transformation process promoted by oncogenes. Although several genes are known to be positively regulated by p53, it is unclear how many gene expression events are involved in p53-mediated growth arrest and apoptosis. Two-dimensional gel electroph...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：:Gallic acid induced apoptotic cell death in human promyelocytic leukemia HL-60 cells. The effect of gallic acid was significantly reduced by blocking the free hydroxyl or carboxyl group with acetyl, methyl, ethyl, n-propyl or isoamyl group. This was paralleled with the decrease in the intensity of both gallate radical...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:5-Fluoro-2'-deoxyuridine (5-FdU), a drug against gastric cancer, was covalently linked via its nucleobase with the amino-bisphosphonate alendronate (Ale), resulting in a new antimetabolite-bisphosphonate conjugate (5-FdU-Ale), designed for bone-targeting. MATERIALS AND METHODS:The cytostatic effect of 5-FdU...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Patients with type 2 diabetes mellitus (T2DM) as well as patients with obesity have increased cancer mortality. In a previous paper we suggested that there was a trend for decreased mortality in obese individuals with T2DM. The aim of the new analyses was to investigate the same relationship after increasing...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND:Mistletoe lectins (MLs) are the active components of aqueous mistletoe extracts widely used in complementary cancer therapy, however, it is not clear if they bind to carbohydrate residues only or whether they interact with proteins as well. Protein-protein interactions do not seem unlikely as MLs act at very...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：BACKGROUND:Macrophage colony stimulating factor (M-CSF) binds to colony-stimulating factor-1 receptor (CSF-1R) and thereby stimulates the proliferation, differentiation and behaviour of monocytes, macrophages and their bone marrow progenitors. Previous studies have suggested that high expression of these markers is cor...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：:This report describes a precise molecular analysis of a rare case of Philadelphia chromosome (Ph) positive acute myeloid leukemia (AML) (FAB classification M2). Phenotypic markers were positive for cells of the myeloid lineage, but negative for B cell and T cell lineage. The leukemic cells carried a Philadelphia chrom...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:The mammalian target of rapamycin (mTOR) controls cell growth through protein synthesis regulation. It can be activated by protein kinase B (AKT) or through ribosomal S6 kinase (S6K1). In malignant glioma, mTOR inhibitors have antiproliferative and proapoptotic effects and mTOR has been suggested as a target...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：BACKGROUND:MicroRNAs (miRNAs), which are endogenously expressed regulatory noncoding RNAs, have an altered expression in tumor tissues. MiRNAs regulate cancer-related processes such as cell growth and tissue differentiation, and therefore, might function as oncogenes or tumor-suppressor genes. The aim of our study was ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：:The purpose of this study was to elucidate the behavior of signal transduction activity in rat and human carcinoma cells. Signal transduction activity was measured by the steady-state activity of the three enzymes involved in the conversion of 1-phosphatidylinositol (PI) to IP3, PI 4-kinase, PI 4-phosphate 5-kinase, a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：BACKGROUND:Transient hypocalcaemia is a frequent complication after total knee arthroplasty (TKA). In this study, we investigated the factors associated with the development of hypocalcaemia after TKA in order to explore its clinical significance and treatment. PATIENTS AND METHODS:A retrospective analysis of the chan...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-03-01 00:00:00
abstract：:Throughout the world, breast cancer is among the major causes of cancer-related death and is the most common cancer found in women. The development of cancer molecular knowledge has surpassed the novel concept of cancer biology and unraveled principle targets for anticancer drug developments and treatment strategies. ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.12502
更新日期：2018-05-01 00:00:00
abstract：:P-glycoprotein has been identified in mast cells stabilized in culture as well as in rat peritoneal mast cells, and is primarily concentrated on the granular membrane. This study aimed to define the role of this protein in the transport and accumulation of doxorubicin in mast cell granules and in its histamine releasi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:The clinical features of 10 cases with the adjunct of a literature review of primary neuroendocrine carcinoma of the skin (Merkel cell tumor) are reported. This cancer arises in the dermis and subcutaneous tissue of elderly individuals. Natural history is characterized by local recurrences (40%), regional lymph-nodes ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:The aim of this study was to investigate the effect of breast cancer adjuvant therapies on the levels of circulating surfactant protein-D (SP-D), C-Reactive protein (CRP) and soluble receptor for advanced glycation end-products (sRAGE), as potential biomarkers of subclinical pulmonary inflammation. MATERIAL...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
abstract：:In the present study, we have investigated the effect of cytotropic heterogeneous molecular lipid (CHML), a new anticancer agent, on growth suppression in a variety of human tumor cell lines. At a non-toxic concentration (a range from 25 micrograms/ml to 100 micrograms/ml), CHML has shown to strongly inhibit tumor cel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
