Abstract：
BACKGROUND:MicroRNAs (miRNAs), which are endogenously expressed regulatory noncoding RNAs, have an altered expression in tumor tissues. MiRNAs regulate cancer-related processes such as cell growth and tissue differentiation, and therefore, might function as oncogenes or tumor-suppressor genes. The aim of our study was to assess the expression of mir-20a, let-7a, miR-15a and miR-16 in prostate cancer (PCa) and benign prostatic hyperplasia (BPH) tissue and to investigate the relation between the expression of miRNAs and the clinicopathological features of PCa. PATIENTS AND METHODS:The study group comprised 138 patients: 85 patients with BPH and 53 patients with PCa. The total RNA was isolated from the tissue specimen core and miRNA expressions were quantified using a real-time RT-PCR method (TaqMan MicroRNA Assays). U6snRNA was used for the normalization of the miRNA expression. RESULTS:miR-20a expression was significantly higher in the group of patients with a Gleason score of 7-10 in comparison with the group of patients with a Gleason score of 0-6 (p=0.0082). We found no statistical differences in the miRNA expressions (mir-20a, let-7a, miR-15a and miR-16) in the PCa tissue samples in comparison with the BPH tissue samples. CONCLUSION:Our result shows that the more dedifferentiated PCa cells have a higher expression of miR-20a and this supports the oncogenic role of miR-20a in PCa carcinogenesis. The evaluation of miRNA expression could yield new information about PCa pathogenesis.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Pesta M,Klecka J,Kulda V,Topolcan O,Hora M,Eret V,Ludvikova M,Babjuk M,Novak K,Stolz J,Holubec Lsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
3579-83issue
9eissn
0250-7005issn
1791-7530pii
30/9/3579journal_volume
30pub_type
杂志文章abstract：:This study investigates the treatment management and survival of inoperable advanced non-small cell lung cancer (NSCLC) patients. The objective was to treat all patients with induction chemotherapy and then to stratify them for surgery, radiotherapy, second-line chemotherapy or supportive treatment. Of the 359 patient...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND/AIM:Hepatic venous isolation and extracorporeal charcoal hemoperfusion (HVI-CHP) can reduce systemic exposure to hepatic arterial infusion (HAI) chemotherapy. The pre-existing HVI-CHP system has limited effectiveness against high-dose cisplatin; therefore, we designed a new system and evaluated its efficacy ...
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journal_title：Anticancer research
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doi：
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journal_title：Anticancer research
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doi：
更新日期：2015-12-01 00:00:00
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journal_title：Anticancer research
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journal_title：Anticancer research
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更新日期：1996-07-01 00:00:00
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doi：
更新日期：2011-06-01 00:00:00
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doi：
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pub_type： 临床试验,杂志文章,评审
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