Abstract：
BACKGROUND/AIM:Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms, and therapeutic options for pNETs are limited. Metformin is an anti-hypoglycemic drug that appears to have anticancer effects. However, little is known about the effect of metformin on pNETs. In this study, we investigated the anti-proliferative effect of metformin on a human pNET cell line. MATERIALS AND METHODS:The anti-proliferative properties of metformin were evaluated in QGP-1 and NCI-H727 cells using a cell counting kit-8 assay. Xenograft mouse models were used to assess the tumor effect in vivo. RESULTS:Metformin inhibited the proliferation and anti-tumor growth of QGP-1 cells, accompanied by their arrest during the cell cycle at the G0/G1 phase. Immunohistochemical analysis of tumor tissues revealed down-regulation of cyclin D1 and proliferating cell nuclear antigen in the metformin-treated group. Additionally, metformin induced apoptosis, and the expression of survivin and claspin were decreased in metformin-treated QGP-1 cells according to the apoptosis array. Furthermore, the angiogenic related protein TIMP-1 was down-regulated, and its miRNA expression was altered by metformin in QGP-1 cells. CONCLUSION:Taken together, our study demonstrated the therapeutic potential of metformin and provides molecular mechanistic insights into its anti-tumoral effect on pNETs. This study is the first one describing anti-tumoral effects in pNETs.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Yamana H,Kato K,Kobara H,Fujihara S,Fujita K,Namima D,Fujita N,Kobayashi K,Kamada H,Morishita A,Tsutsui K,Iwama H,Masaki Tdoi
10.21873/anticanres.13933subject
Has Abstractpub_date
2020-01-01 00:00:00pages
121-132issue
1eissn
0250-7005issn
1791-7530pii
40/1/121journal_volume
40pub_type
杂志文章abstract：BACKGROUND:The possibilities and limitations of computer tomography (CT) examination for detecting primary gastric cancer tumors have been poorly understood. The aim of the present study was to evaluate the pre-operative assessment of gastric cancer tumors using 32-multidetector row-CT. PATIENTS AND METHODS:A prospect...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：UNLABELLED:Adenomatous polyps are precursor lesions for colorectal carcinoma. The risk of cancer development has been associated with age and size, amount of villous component and high-grade dysplasia of adenomas. The subject-related and adenoma-related risk factors for severely dysplastic lesions were further investig...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-09-01 00:00:00
abstract：:Three histamine H2-receptor antagonists, Cimetidine, Metiamide and Ranitidine, were tested for their inhibitory effect on two experimental bowel cancer models. In the first model mitotic rates were measured in dimethylhydrazine-induced tumors of rat colon and in the second model volumetric changes in human large bowel...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:TNF-alpha is one of the key inflammatory cytokines and it modulates various events through several pathways. U937 myelomonocytic leukemia cells are sensitive to TNF-alpha and about 20% of these cells undergo apoptosis within 6 hours after treatment. Co-treatment of these cells with actinomycin D or cyclohexi...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND:Laparoscopic pelvic lymph node staging is widely used in patients with cervical cancer prior to the initiation of primary chemoradiation therapy. Data on the morbidity of this procedure are sparse. PATIENTS AND METHODS:Between 1995 and 2007, 71 patients with locally advanced cervical cancer (FIGO stage IB2-...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:Paclitaxel pharmacokinetics were shown to be related to toxicity and survival. PATIENTS AND METHODS:We evaluated the effects of time above paclitaxel concentrations of 0.05 micromol/l (T(>0.05) and systemic exposures (AUC) to total and unbound paclitaxel (tPAC, uPAC) on response in patients with advanced ca...
journal_title：Anticancer research
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2005-11-01 00:00:00
abstract：:In furtherance of our structure-activity relationship studies on the antitumor activity of indolo[2,3-b]quinolines, novel cytotoxic derivatives bearing methyl groups at N-5, C-11, C-2 and/or C-9, as well as methoxy-groups at C-2 and/or C-9, were synthesized by the modified Graebe-Ullmann reaction. To elucidate the met...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：AIM:The current study aimed to determine the prognostic significance of thrombocytosis in patients with colorectal cancer (CRC) by a meta-analysis of the literature. PATIENTS AND METHODS:The meta-analysis followed the 2009 guidelines of Preferred Reporting Items for Systematic Reviews and Meta-Analyses. A systematic l...
journal_title：Anticancer research
pub_type： 杂志文章,meta分析,评审
doi：10.21873/anticanres.11878
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND:Epstein-Barr virus-associated gastric carcinoma (EBV-GC) has been characterized as a special gastric cancer subset. Lifestyle and other major factors that may contribute to EBV-GC and non-EBV-GC were investigated here. MATERIALS AND METHODS:A total of 247 patients with gastric cancer were interviewed, clini...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-06-01 00:00:00
abstract：UNLABELLED:Magnetic drug targeting (MDT) is a new locoregional chemotherapy method that increases the drug dose in the tumour region, while simultaneously reducing the overall dose through the application of chemotherapeutic-bound superparamagnetic nanoparticles, which are focused by an external magnetic field to the d...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-05-01 00:00:00
abstract：BACKGROUND:Mistletoe lectins (MLs) are the active components of aqueous mistletoe extracts widely used in complementary cancer therapy, however, it is not clear if they bind to carbohydrate residues only or whether they interact with proteins as well. Protein-protein interactions do not seem unlikely as MLs act at very...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:The option to treat patients presenting with HER-2 overexpressed invasive breast carcinoma with Herceptin requires quantitative determination of the HER-2 status. The aim of this study was to retrospectively evaluate the HER-2 mRNA expression levels using quantitative real-time RT-PCR (Q-RT-PCR) in tissue samples from...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：BACKGROUND:The altered expression of multidrug resistance 1 (MDR1) contributes to various types of carcinogenesis and their disease progression. Hypermethylation of CpG islands located in the promoter regions is involved in gene silencing at the transcriptional level. The methylation status of MDR1 gene promoter was in...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:CD62L is the human homologue of the murine lymphocyte homing receptor, mel-14. We investigated CD62L + cells in the spleen from patients with gastric cancer. Flow cytometric analysis revealed that CD62L + cells were decreased in the peripheral blood, but inversely increased in the spleen in parallel with disease progr...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：BACKGROUND/AIM:Ouabain, a plant-derived product/substance with Na+/K+-ATPase inhibiting properties, has been shown to exert anti-cancer activity on human cancer cells. This is the first study to investigate the effect of ouabain on apoptotic cell death of human osteosarcoma-derived U-2 OS cells. MATERIALS AND METHODS:...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12205
更新日期：2018-01-01 00:00:00
abstract：:In a retrospective study, immunohistochemical determinations of p53 were performed on paraffinised tissue sections of 243 patients with node negative breast cancer. These patients were primary treated in the years 1980-1986 at the UFK-Würzburg. A complete follow up could be performed in 92% of all cases. The median fo...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Decreased expression of VDUP1, which is an interesting stress response gene, has been shown in rat mammary tumors and has been discussed in relation to the development of the tumor. However, VDUP1 expression in clinical specimens of human cancer remains unclear. We employed TaqMan RT-PCR assay to investigate VDUP1 exp...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：BACKGROUND:Polymorphisms altering DNA repair capacity may lead to synergistic effects with tobacco carcinogen-induced lung cancer risk. Based on this hypothesis, the relationship between APE1 polymorphism, smoking and the risk of lung cancer was explored. MATERIALS AND METHODS:The distribution of the APE1 Asp148Glu po...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2009-06-01 00:00:00
abstract：:Hepatocellular carcinoma is one of the most common malignancies in the world, with the liver being the second most frequently involved organ in metastatic disease. Although the gold standard treatment for malignant liver disease is surgical resection, only few patients can undergo such an intervention. This explains t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-07-01 00:00:00
abstract：BACKGROUND:Pulmonary complications (PCs) after esophagectomy for patients with esophageal cancer have been correlated with prolonged hospital stays and in-hospital mortality. Previous studies have shown that minimally-invasive esophagectomy (MIE) is associated with a lower rate of PCs compared to conventional open surg...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2014-05-01 00:00:00
abstract：:Adult lymphoblastic lymphoma (LBL) can be of T-cell or B-cell lineage. However, the clinical significance of immunophenotypes is largely unknown. We conducted a retrospective study to compare T-cell LBL with its B-cell counterpart. Between 1983 and 1995, 50 adult patients were diagnosed as LBL at National Taiwan Unive...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：BACKGROUND:Angiogenesis is required for tumor growth and metastasis, and is an exciting target for cancer treatment. We designed and synthesized antiangiogenetic TX agent (TX-1898, -1900), and analyzed their structural features. TXs have a chiral center and S- and R-enantiomers. Conformation analysis and molecular dyna...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND:Once diagnosed, esophageal cancer has a very low overall 5-year survival rate. This study investigates the mechanisms behind the invasiveness and severity of esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS:Transwell invasion chamber was used to subdivide one Taiwanese ESCC cell line, CE81T/...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：:Grape seed extracts were more cytotoxic than grape peel extracts. Methanol and 70% methanol extracts of grape seed selectively killed two human oral tumor cell lines, more efficiently than human gingival fibroblasts. ESR spectroscopy revealed that these extracts produced radicals under alkaline conditions and enhanced...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：BACKGROUND:The size of colorectal polyps is important in the clinical management of these lesions. When using a conventional ruler (the tool of pathologists worldwide), we have previously found unacceptably high intra- and inter-observer variations in assessing the size of phantom polyps. The aim of this study was to a...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND/AIM:SLC9A9 plays an oncogenic role in esophageal squamous carcinoma and glioblastoma. Herein, we showed an oncogenic function of SLC9A9 in colorectal cancer (CRC). MATERIALS AND METHODS:We examined SLC9A9 expression in CRC specimens by immunohistochemistry. In CRC tissues, the relationship between SLC9A9 ex...
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.11562
更新日期：2017-05-01 00:00:00
abstract：:Catalytic activity and FdUMP binding characteristics of thymidylate synthase (TS) were determined in 22 tumor biopsies of patients to be treated (15) or just treated (7) for colorectal cancer with 5-fluorouracil and leucovorin. In 19 samples both parameters could be determined and were found to represent a wide range ...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND:S100(+) dendritic cells and CD57(+) lymphocytes are factors reflecting the immune system's ability to suppress the progress of tumor growth. CD57(+) cells include natural killer cells and late stages of T-effector lymphocytes. We evaluated the relationship between the known clinical and histological factors ...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-05-01 00:00:00
abstract：:Overexpression of p53-protein appears to be a common event in primary breast cancer. It has been proposed that the presence of elevated levels of this protein may be an independent prognostic factor and may be important for the ability of a tumor to metastasize. This study was performed to evaluate the influence of im...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：BACKGROUND:Preclinical studies have shown that paclitaxel and doxifluridine can act synergistically without overlapping toxicity for the treatment of advanced gastric cancer. The objectives of this study were to determine the maximum tolerated dose (MTD), the dose-limiting toxicity and the recommended Phase II dose for...
journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：2005-03-01 00:00:00
