Differential effect of oleic and palmitic acid on lipid accumulation and apoptosis in cultured hepatocytes.

Abstract:

BACKGROUND AND AIM:Studies have shown monounsaturated oleic acid to be less toxic than palmitic acid and to prevent/attenuate palmitic acid hepatocites toxicity in steatosis models in vitro. However, to what degree these effects are mediated by steatosis extent is unknown. METHODS:We evaluated whether steatosis per se is associated with hepatocytes apoptosis and determined the role of oleic and palmitic acid, the most abundant fatty acids in western diets, on triglyceride accumulation and apoptosis in an in vitro model of steatosis induced in three hepatocytic cell lines (HepG2, HuH7, WRL68). The impact of incubation for 24 h with oleic (0.66 and 1.32 mM) and palmitic acid (0.33 and 0.66 mM), alone or combined (molar ratio 2 : 1) on steatosis, apoptosis, and insulin signalling, was evaluated. RESULTS:Concurrent with PPARgamma and SREBP-1 gene activation, steatosis extent was larger when cells were treated with oleic than with palmitic acid; the latter fatty acid was associated with increased PPARalpha expression. Cell apoptosis was inversely proportional to steatosis deposition. Moreover, palmitic, but not oleic acid, impaired insulin signalling. Despite the higher amount of fat resulting from incubation of the two fatty acids combined, the apoptosis rate and impaired insulin signalling were lower than in cells treated with palmitic acid alone, indicating a protective effect of oleic acid. CONCLUSIONS:Oleic acid is more steatogenic but less apoptotic than palmitic acid in hepatocityc cell cultures. These data may provide a biological basis for clinical findings on dietary patterns and pathogenetic models of nonalcoholic fatty liver disease.

authors

Ricchi M,Odoardi MR,Carulli L,Anzivino C,Ballestri S,Pinetti A,Fantoni LI,Marra F,Bertolotti M,Banni S,Lonardo A,Carulli N,Loria P

doi

10.1111/j.1440-1746.2008.05733.x

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

830-40

issue

5

eissn

0815-9319

issn

1440-1746

pii

JGH5733

journal_volume

24

pub_type

杂志文章
  • Clinicopathological features of patients with acute graft-versus-host disease of the upper digestive tract.

    abstract:BACKGROUND AND AIMS:Acute graft-versus-host disease (GVHD) occurring within 100 days post-transplant is one of critical factors influencing prognosis in transplant recipients. Among cases of GVHD of the gastrointestinal (GI) tract, GVHD rarely affects the upper GI. In this study, we retrospectively examined the frequen...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.12651

    authors: Nomura K,Iizuka T,Kaji D,Yamamoto H,Kuribayashi Y,Kimura R,Yamada A,Furuhata T,Yamashita S,Kikuchi D,Matsui A,Mitani T,Ogawa O,Hoteya S,Ota Y,Taniguchi S,Kaise M

    更新日期:2014-11-01 00:00:00

  • Clinical impact of HFE mutations in Japanese patients with chronic hepatitis C.

    abstract:BACKGROUND AND AIM:HFE mutations, a common cause of hereditary hemochromatosis (HH), are reportedly associated with hepatic iron overload, severe liver fibrosis, and good response to interferon treatment in European patients with chronic hepatitis C (CHC). HH shows ethnicity-based differences and little is known about ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2011.06976.x

    authors: Ishizu Y,Katano Y,Honda T,Hayashi K,Ishigami M,Itoh A,Hirooka Y,Nakano I,Goto H

    更新日期:2012-06-01 00:00:00

  • Natural history of acute hepatitis C.

    abstract::To study the short- and long-term outcomes of acute hepatitis C, three groups of patients were enrolled. Of 26 patients with acute hepatitis C, 18 (69%) maintained HCV viraemia and 8 had cleared virus naturally at 12 months after the onset. Normalization of ALT was seen in all 8 patients with acute resolving hepatitis...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1746.2000.02112.x

    authors: Tanaka E,Kiyosawa K

    更新日期:2000-05-01 00:00:00

  • Childhood pancreatitis.

    abstract::Inflammation of the pancreas has many presentations in children and adolescents. The etiology is often elusive, with a great number of cases being idiopathic. However, there have been a number of recent advances in the areas of cell biology, genetics and imaging technology, which should be highlighted. Herein is provi...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1746.2006.04246.x

    authors: Nydegger A,Couper RT,Oliver MR

    更新日期:2006-03-01 00:00:00

  • Therapeutic efficacy of dendritic cell vaccine combined with programmed death 1 inhibitor for hepatocellular carcinoma.

    abstract:BACKGROUND AND AIM:Hepatocellular carcinoma (HCC) remains a serious cause of cancer-related deaths worldwide. Developing new therapeutic strategies is urgently needed to improve the outcomes of HCC patients. Dendritic cell (DC)-based vaccines and programmed death 1 (PD-1) immune checkpoint inhibitors have been regarded...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.15398

    authors: Teng CF,Wang T,Shih FY,Shyu WC,Jeng LB

    更新日期:2021-01-19 00:00:00

  • Lamivudine/pegylated interferon alfa-2b sequential combination therapy compared with lamivudine monotherapy in HBeAg-negative chronic hepatitis B.

    abstract:BACKGROUND AND AIM:Monotherapy has been proven insufficient in achieving sustained control of chronic hepatitis B. We aimed to assess the efficacy of combined sequential administration of lamivudine and pegylated interferon alfa-2b in patients with hepatitis Be antigen (HBeAg)-negative chronic hepatitis B. METHODS:Eig...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2007.05103.x

    authors: Vassiliadis T,Tziomalos K,Patsiaoura K,Zagris T,Giouleme O,Soufleris K,Grammatikos N,Theodoropoulos K,Mpoumponaris A,Dona K,Zezos P,Nikolaidis N,Orfanou-Koumerkeridou E,Balaska A,Eugenidis N

    更新日期:2007-10-01 00:00:00

  • Functional gastrointestinal disorders and overlap syndrome in Korea.

    abstract::Functional gastrointestinal disorders (FGIDs) are common in clinical practice and in communities around the world, including Korea. In a recent point prevalence study on functional dyspepsia (FD) in Korea using the Rome III criteria, 13.4% of community respondents reported dyspepsia. Forty-seven percent of these FD ca...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2011.06644.x

    authors: Park H

    更新日期:2011-04-01 00:00:00

  • KRAS mutation analysis by next-generation sequencing in endoscopic ultrasound-guided sampling for solid liver masses.

    abstract:BACKGROUND AND AIM:KRAS mutation analysis may provide ancillary diagnostic yield in an endoscopic ultrasound (EUS)-guided histopathological evaluation. We evaluated the clinical impact of KRAS mutation analysis with cytological and histological evaluations in EUS-guided tissue sampling, using a core biopsy needle for s...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.13423

    authors: Choi HJ,Moon JH,Kim HK,Lee YN,Lee TH,Cha SW,Cho YD,Park SH

    更新日期:2017-01-01 00:00:00

  • Repeating measurements by transient elastography in non-alcoholic fatty liver disease patients with high liver stiffness.

    abstract:BACKGROUND AND AIM:The Baveno VI Consensus recommends repeating examination in patients with high liver stiffness measurement (LSM) by transient elastography to reduce false-positive diagnosis of advanced liver disease. We tested whether repeating transient elastography can increase the overall diagnostic accuracy. ME...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14311

    authors: Chow JC,Wong GL,Chan AW,Shu SS,Chan CK,Leung JK,Choi PC,Chim AM,Chan HL,Wong VW

    更新日期:2019-01-01 00:00:00

  • Homology model of the DNA gyrase enzyme of Helicobacter pylori, a target of quinolone-based eradication therapy.

    abstract:BACKGROUND AND AIMS:Resistance of Helicobacter pylori to the standard therapeutic antimicrobial agents has been demonstrated. Although quinolones are an alternative candidate for third-line eradication therapy, quinolone resistance of H. pylori is also increasing. Quinolone resistance of H. pylori is caused by a point ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2010.06245.x

    authors: Matsuzaki J,Suzuki H,Tsugawa H,Nishizawa T,Hibi T

    更新日期:2010-05-01 00:00:00

  • Non-alcoholic fatty liver disease in the Asia-Pacific region: definitions and overview of proposed guidelines.

    abstract::Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in Western industrialized countries, affecting 20-40% of the general population. Large population-based surveys in China, Japan, and Korea indicate that the prevalence of NAFLD is now 12% to 24% in population subgroups, depending on age, gende...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2007.05001.x

    authors: Chitturi S,Farrell GC,Hashimoto E,Saibara T,Lau GK,Sollano JD,Asia-Pacific Working Party on NAFLD.

    更新日期:2007-06-01 00:00:00

  • Microcirculatory alteration in low-grade gastric mucosa-associated lymphoma by Helicobacter heilmannii infection: its relation to vascular endothelial growth factor and cyclooxygenase-2.

    abstract:BACKGROUND:There are clinical reports that Helicobacter heilmannii, as well as Helicobacter pylori, has been clinically reported to cause gastric low-grade mucosa-associated lymphoid tissue-type (MALT) lymphoma, although its precise mechanism remains to be clarified. Thus, the present study was undertaken to elucidate ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2008.05554.x

    authors: Nakamura M,Takahashi S,Matsui H,Murayama SY,Aikawa C,Sekiya Y,Nishikawa K,Matsumoto T,Yamada H,Tsuchimoto K

    更新日期:2008-12-01 00:00:00

  • Use of transient elastography in patients with HIV-HCV coinfection: A systematic review and meta-analysis.

    abstract:BACKGROUND AND AIM:Patients with HIV-hepatitis C virus (HCV) coinfection progress towards liver fibrosis and cirrhosis more rapidly compared with HCV mono-infected individuals. This necessitates an accurate assessment of liver stiffness with transient elastography to guide treatment. METHODS:Searches of PubMed, EMBASE...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,meta分析,评审

    doi:10.1111/jgh.13337

    authors: Njei B,McCarty TR,Luk J,Ewelukwa O,Ditah I,Lim JK

    更新日期:2016-10-01 00:00:00

  • Treatment with a novel lipid A analogue, FS-112, and partial hepatectomy causes submassive liver necrosis and impaired liver regeneration in mice.

    abstract::A novel experimental model of submassive liver necrosis with impaired regeneration has been established. A novel lipid A analogue, FS-112, was injected intravenously into male BALB/c mice, followed 2 days later by a 70% partial hepatectomy. Over the next 9 days, mice became severely jaundiced, with a peak total biliru...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1996.tb00293.x

    authors: Maeda T,Ohnishi H,Hayashi T,Ishiki Y,Moriwaki H,Muto Y

    更新日期:1996-05-01 00:00:00

  • Characteristics of belching, swallowing, and gastroesophageal reflux in belching patients based on Rome III criteria.

    abstract:BACKGROUND AND AIMS:Belching is a common disorder with undetermined pathogenesis. With the combined multichannel intraluminal impedance pH monitoring, two different models of belching have been defined: gastric belching (GB) and supragastric belching (SB). The aim of this study was to assess whether SB was associated w...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.12187

    authors: Li J,Xiao Y,Peng S,Lin J,Chen M

    更新日期:2013-08-01 00:00:00

  • Contrast media-enhanced power Doppler sonography for evaluation of hemangiomas and malignant tumors in the liver.

    abstract:AIM:To evaluate the characterization of liver hemangiomas and malignant tumors using power Doppler sonography before and after intravenous injection of a sonocontrast agent. METHODS:Forty-five patients with 57 liver tumors (22 hemangiomas, 24 metastases, 10 hepatocellular carcinomas (HCC) and one cholangiocellular car...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1746.2003.02900.x

    authors: Karabacakoglu A,Karakose S,Cil AS,Kaya A

    更新日期:2003-01-01 00:00:00

  • Morphological comparison and functional reconstitution of rat hepatic parenchymal cells on various matrices.

    abstract::Four types of materials, type I collagen coat (Coat), acid-soluble type I collagen gel (Hardgel), pepsin-treated acid-soluble type I collagen gel (Softgel), and an extract of extracellular matrix of the murine Engelbreth-Holm-Swarm sarcoma (Matrigel), were used as matrices to culture rat hepatic parenchymal cells, and...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.1998.13.s1.55

    authors: Awata R,Sawai H,Imai K,Terada K,Senoo H,Sugiyama T

    更新日期:1998-09-01 00:00:00

  • Case report: lymphangioma of the oesophagus endoscopically resected.

    abstract::Lymphangioma of the oesophagus is an extremely rare entity, with only nine cases having been reported worldwide. We report on a 52-year-old woman with oesophageal lymphangioma, diagnosed using endoscopic ultrasonography and endoscopically resected. No case of malignant transformation of the lymphangioma has been repor...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1996.tb00333.x

    authors: Suwa T,Ozawa S,Ando N,Shinozaki H,Tsujitsuka K,Miki H,Makuuchi H,Kitajima M

    更新日期:1996-08-01 00:00:00

  • Association between the methylation of the STAT1 and SOCS3 in peripheral blood and gastric cancer.

    abstract:BACKGROUND AND AIM:DNA methylation is an important epigenetic modification that can promote the development of various cancers. The STAT1 and SOCS3 have been observed to be hypermethylated in tumor tissues and peripheral blood. This study aimed to explore the relationship between the methylation status of the STAT1 and...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.15021

    authors: Han Q,Zhou H,Xie W,Sun T,Wei R,Nie C,Hong J,Zhu L,Tian W

    更新日期:2020-08-01 00:00:00

  • Is apoptosis in antral mucosa correlated with serum nitrite concentration in Japanese Helicobacter pylori-infected patients?

    abstract:BACKGROUND AND AIM:Helicobacter pylori infection in gastric mucosa is a form of chronic active gastritis that leads to expression of inducible nitric oxide synthase in host macrophages and polymorphonuclear leukocytes. Nitric oxide produced by these cells infiltrating the gastric mucosa could damage DNA. We correlated ...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1746.2003.03013.x

    authors: Tari A,Kodama K,Kitadai Y,Ohta M,Sumii K,Kajiyama G

    更新日期:2003-05-01 00:00:00

  • Validation of European evidence-based guidelines and American College of Gastroenterology guidelines as predictors of advanced neoplasia in patients with suspected mucinous pancreatic cystic neoplasms.

    abstract:BACKGROUND AND AIM:The European evidence-based guidelines (EEG) and American College of Gastroenterology Guidelines (ACGG) have been published to guide the management of pancreatic cystic lesions. We aim to evaluate the value of both guidelines in predicting advanced pancreatic cystic lesions (A-PCLs) with preoperative...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14973

    authors: Sun L,Wang W,Wang Y,Jiang F,Peng L,Jin G,Jin Z

    更新日期:2020-09-01 00:00:00

  • Molecular adsorbent recirculating system: albumin dialysis-based extracorporeal liver assist device.

    abstract::Extracorporeal liver assist devices have been used for more than five decades to support patients with liver failure. Numerous modifications have been made to both biological as well as mechanical liver assist devices. Possibly, an ideal liver assist device would be one that would perform optimal detoxification and sy...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1440-1746.17.s3.14.x

    authors: Kapoor D

    更新日期:2002-12-01 00:00:00

  • Segmental colonoscopic biopsies in the differentiation of ileocolic tuberculosis from Crohn's disease.

    abstract:BACKGROUND AND AIM:The differentiation between Crohn's disease (CD) and tuberculosis (TB) of the intestine can be difficult in areas where both diseases occur. The present study examined histological criteria that would enable the diagnosis in mucosal biopsies. METHODS:Colonoscopic biopsies from 33 patients with TB an...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2005.03814.x

    authors: Pulimood AB,Peter S,Ramakrishna B,Chacko A,Jeyamani R,Jeyaseelan L,Kurian G

    更新日期:2005-05-01 00:00:00

  • Clinical characterization and mutation spectrum in patients with familial adenomatous polyposis in China.

    abstract:BACKGROUND AND AIM:Familial adenomatous polyposis (FAP) is the most common adenomatous polyposis syndrome. Patients with FAP are screened for germline mutations of two genes, APC and MUTYH. However, limited data exist on the clinical characterization and genotypic spectrum of FAP in China. This study was aimed to deter...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/jgh.14704

    authors: Li N,Kang Q,Yang L,Zhao XJ,Xue LJ,Wang X,Li AQ,Li CG,Sheng JQ

    更新日期:2019-09-01 00:00:00

  • Health-related quality of life in Chinese patients with primary biliary cirrhosis.

    abstract:BACKGROUND AND AIM:Symptoms and complications of primary biliary cirrhosis (PBC) have been shown to impair patients' health-related quality of life (HRQOL) in the West. We aimed to measure the HRQOL and to determine the factors associated with worse HRQOL among the Chinese PBC patients in Hong Kong. METHODS:Chinese pa...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2007.05092.x

    authors: Wong GL,Law FM,Wong VW,Hui AY,Chan FK,Sung JJ,Chan HL

    更新日期:2008-04-01 00:00:00

  • Ki-67 expression as a prognostic marker in patients with hepatocellular carcinoma.

    abstract::Ki-67 expression in tumours has been shown to be associated with prognosis in patients with hepatocellular carcinoma (HCC). In this study, primary HCC samples were obtained from 67 patients undergoing surgical resection. None of these patients had been subjected previously to any other form of therapy, such as arteria...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.1998.01555.x

    authors: King KL,Hwang JJ,Chau GY,Tsay SH,Chi CW,Lee TG,Wu LH,Wu CW,Lui WY

    更新日期:1998-03-01 00:00:00

  • Asia Pacific Consensus Statements on Crohn's disease. Part 1: Definition, diagnosis, and epidemiology: (Asia Pacific Crohn's Disease Consensus--Part 1).

    abstract::Inflammatory bowel disease (IBD) was previously thought to be rare in Asia, but emerging data indicate rising incidence and prevalence of IBD in the region. The Asia Pacific Working Group on Inflammatory Bowel Disease was established in Cebu, Philippines, at the Asia Pacific Digestive Week conference in 2006 under the...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1111/jgh.12956

    authors: Ooi CJ,Makharia GK,Hilmi I,Gibson PR,Fock KM,Ahuja V,Ling KL,Lim WC,Thia KT,Wei SC,Leung WK,Koh PK,Gearry RB,Goh KL,Ouyang Q,Sollano J,Manatsathit S,de Silva HJ,Rerknimitr R,Pisespongsa P,Abu Hassan MR,Sung J,

    更新日期:2016-01-01 00:00:00

  • Risk of alanine aminotransferase flare-up among asymptomatic hepatitis C virus RNA carriers: a 10-year follow-up study.

    abstract:AIMS:The aim of this study was to determine the cumulative rate of flare-up of serum alanine aminotransferase (ALT) level during a 10-year follow-up period, and characterize the clinical, virologic features in 120 hepatitis C virus (HCV) RNA-positive asymptomatic carriers with persistently normal ALT levels for 6 month...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1046/j.1440-1746.2001.02474.x

    authors: Tsuji K,Yamasaki K,Yamanishi M,Kawakami M,Shirahama S

    更新日期:2001-05-01 00:00:00

  • Transcriptional regulation of stellate cell activation.

    abstract::The identification of activated hepatic stellate cells and related cell types as key fibrogenic effectors during liver injury has led to intense evaluation of transcriptional events underlying their behavior. While initial studies focused on characterizing interactions between transcription factors and regulatory regi...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1440-1746.2006.04585.x

    authors: Friedman SL

    更新日期:2006-10-01 00:00:00

  • Adefovir dipivoxil therapy in liver transplant recipients for recurrence of hepatitis B virus infection despite lamivudine plus hepatitis B immunoglobulin prophylaxis.

    abstract:BACKGROUND:Treatment of post-transplantation recurrence of hepatitis B virus (HBV) infection despite prophylaxis with hepatitis B immunoglobulin (HBIG) and lamivudine combination therapy is not easy. Because HBV reinfection has a severe course and could result in graft failure in liver transplant recipients, prompt med...

    journal_title:Journal of gastroenterology and hepatology

    pub_type: 杂志文章

    doi:10.1111/j.1440-1746.2006.04609.x

    authors: Akyildiz M,Karasu Z,Zeytunlu M,Aydin U,Ozacar T,Kilic M

    更新日期:2007-12-01 00:00:00