Abstract:
:Benzene is volatile organic hydrocarbon which is widely used in a wide range of industries. Studies have shown that exposure to benzene consequences serious health risks for human. Understanding the effect and risks of environmental hazard materials in the laser therapy of skin is interesting which can show useful or harmful role of these effects in therapies. In this study, the effect of low-level laser therapy was investigated on benzene-induced cytotoxicity on human skin fibroblast cells (HU02). Human skin fibroblast cells (HU02) were exposed to various concentrations of benzene (0-100 μg/mL) and incubated for 2 h. Then the effect of low-level laser therapy (LLLT) at 660-nm wavelength with 3 J/cm2 energy for 90 s was investigated on the viability of the cells exposed to benzene using MTT assay and inverted light microscope. The effect of low-level laser therapy on the viability of the cells was positive at concentrations 0-15 μg/mL but negative at higher concentrations than 15 μg/mL. Low-level laser therapy in low concentrations of benzene decreases the cytotoxicity caused by benzene and maintains cell viability. At high concentrations and in the presence of low-level laser therapy, the cell viability decreased compared to dark experiment. The morphology study of the cells using inverted light microscopy has confirmed the MTT results.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Salemi M,Khorsandi K,Hosseinzadeh R,Maghami Pdoi
10.1007/s10103-020-03211-ysubject
Has Abstractpub_date
2021-01-07 00:00:00eissn
0268-8921issn
1435-604Xpii
10.1007/s10103-020-03211-ypub_type
杂志文章abstract::The objective of this study was to evaluate the microbiological and clinical outcomes following nonsurgical treatment by either scaling and root planing, combination of Nd:YAG and Er:YAG lasers, or by Er:YAG laser treatment alone. The study involved 60 patients with generalized chronic periodontitis, randomly assigned...
journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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pub_type: 杂志文章,随机对照试验
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
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