Reverse optical trawling for synaptic connections in situ.

Abstract:

:We introduce a new method to unveil the network connectivity among dozens of neurons in brain slice preparations. While synaptic inputs were whole cell recorded from given postsynaptic neurons, the spatiotemporal firing patterns of presynaptic neuron candidates were monitored en masse with functional multineuron calcium imaging, an optical technique that records action potential-evoked somatic calcium transients with single-cell resolution. By statistically screening the neurons that exhibited calcium transients immediately before the postsynaptic inputs, we identified the presynaptic cells that made synaptic connections onto the patch-clamped neurons. To enhance the detection power, we devised the following points: 1) [K+]e was lowered and [Ca2+]e and [Mg2+]e were elevated, to reduce background synaptic activity and minimize the failure rate of synaptic transmission; and 2) a small fraction of presynaptic neurons was specifically activated by glutamate applied iontophoretically through a glass pipette that was moved to survey the presynaptic network of interest ("trawling"). Then we could theoretically detect 96% of presynaptic neurons activated in the imaged regions with a 1% false-positive error rate. This on-line probing technique would be a promising tool in the study of the wiring topography of neuronal circuits.

journal_name

J Neurophysiol

authors

Sasaki T,Minamisawa G,Takahashi N,Matsuki N,Ikegaya Y

doi

10.1152/jn.00012.2009

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

636-43

issue

1

eissn

0022-3077

issn

1522-1598

pii

00012.2009

journal_volume

102

pub_type

杂志文章
  • Prolonged vibration of the biceps brachii tendon reduces time to failure when maintaining arm position with a submaximal load.

    abstract::Vibration reduces the amplitudes of the tendon jerk response and the Hoffmann and stretch reflexes in the muscle exposed to the vibration, yet does not alter the time to task failure when the task involves exerting a submaximal force against a rigid restraint. Because the amplitude of the stretch reflex is greater whe...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.00807.2005

    authors: Mottram CJ,Maluf KS,Stephenson JL,Anderson MK,Enoka RM

    更新日期:2006-02-01 00:00:00

  • Superior colliculus signals decisions rather than confidence: analysis of single neurons.

    abstract::Recent findings indicate that monkeys can report their confidence in perceptual decisions and that this information is encoded in neurons involved in making decisions, including the lateral intraparietal area (LIP) and the supplementary eye field (SEF). A key issue to consider when studying confidence is that decision...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00152.2018

    authors: Grimaldi P,Cho SH,Lau H,Basso MA

    更新日期:2018-11-01 00:00:00

  • Silencing-induced metaplasticity in hippocampal cultured neurons.

    abstract::Silencing-induced homeostatic plasticity is usually expressed as a change in the amplitude or the frequency of miniature postsynaptic currents. Here we report that, prolonged (approximately 24 h) silencing of mature (20-22 days in vitro) cultured hippocampal neurons using the voltage-gated sodium channel blocker tetro...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90378.2008

    authors: Sokolova IV,Mody I

    更新日期:2008-08-01 00:00:00

  • Megamap: flexible representation of a large space embedded with nonspatial information by a hippocampal attractor network.

    abstract::The problem of how the hippocampus encodes both spatial and nonspatial information at the cellular network level remains largely unresolved. Spatial memory is widely modeled through the theoretical framework of attractor networks, but standard computational models can only represent spaces that are much smaller than t...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00856.2015

    authors: Hedrick KR,Zhang K

    更新日期:2016-08-01 00:00:00

  • Na+-activated K+ current contributes to postexcitatory hyperpolarization in neocortical intrinsically bursting neurons.

    abstract::The ionic mechanisms underlying the termination of action-potential (AP) bursts and postburst afterhyperpolarization (AHP) in intrinsically bursting (IB) neocortical neurons were investigated by performing intracellular recordings in thin slices of rat sensorimotor cortex. The blockade of Ca(2+)-activated K(+) current...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00695.2002

    authors: Franceschetti S,Lavazza T,Curia G,Aracri P,Panzica F,Sancini G,Avanzini G,Magistretti J

    更新日期:2003-04-01 00:00:00

  • Visuomotor velocity transformations for smooth pursuit eye movements.

    abstract::Smooth pursuit eye movements are driven by retinal motion signals. These retinal motion signals are converted into motor commands that obey Listing's law (i.e., no accumulation of ocular torsion). The fact that smooth pursuit follows Listing's law is often taken as evidence that no explicit reference frame transformat...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1152/jn.00728.2009

    authors: Blohm G,Lefèvre P

    更新日期:2010-10-01 00:00:00

  • EMG changes in human thenar motor units with force potentiation and fatigue.

    abstract::Few studies have analyzed activity-induced changes in EMG activity in individual human motor units. We studied the changes in human thenar motor unit EMG that accompany the potentiation of twitch force and fatigue of tetanic force. Single motor unit EMG and force were recorded in healthy subjects in response to select...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.00924.2005

    authors: Thomas CK,Johansson RS,Bigland-Ritchie B

    更新日期:2006-03-01 00:00:00

  • Drugs affecting phospholipase C-mediated signal transduction block the olfactory cyclic nucleotide-gated current of adult zebrafish.

    abstract::Amino acid and bile salt odorants are detected by zebrafish with relatively independent odorant receptors, but the transduction cascade(s) subsequently activated by these odorants remains unknown. Electro-olfactogram recording methods were used to determine the effects of two drugs, reported to affect phospholipase C ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1998.79.3.1183

    authors: Ma L,Michel WC

    更新日期:1998-03-01 00:00:00

  • Functional unity of the ponto-cerebellum: evidence that intrapontine communication is mediated by a reciprocal loop with the cerebellar nuclei.

    abstract::The majority of cerebral signals destined for the cerebellum are handed over by the pontine nuclei (PN), which thoroughly reorganize the neocortical topography. The PN maps neocortical signals of wide-spread origins into adjacent compartments delineated by spatially precise distribution of cortical terminals and posts...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01060.2005

    authors: Möck M,Butovas S,Schwarz C

    更新日期:2006-06-01 00:00:00

  • Directionally sensitive ganglion cells in the rabbit retina: specificity for stimulus direction, size, and speed.

    abstract::The receptive fields of directionally sensitive ganglion cells in the rabbit retina were analyzed. Several types of experiment showed that each point within the receptive field of the cell is inhibited by a fairly wide area of points around it, lying on each side of the preferred-null axis as well as along the preferr...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1975.38.3.613

    authors: Wyatt HJ,Daw NW

    更新日期:1975-05-01 00:00:00

  • Electrical coupling between Aplysia bag cell neurons: characterization and role in synchronous firing.

    abstract::In neuroendocrine cells, hormone release often requires a collective burst of action potentials synchronized by gap junctions. This is the case for the electrically coupled bag cell neurons in the reproductive system of the marine snail, Aplysia californica. These neuroendocrine cells are found in two clusters, and fi...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00494.2014

    authors: Dargaei Z,Colmers PL,Hodgson HM,Magoski NS

    更新日期:2014-12-01 00:00:00

  • P2 receptor-mediated signaling in spherical bushy cells of the mammalian cochlear nucleus.

    abstract::Purinoreceptors of the P2 family contribute strongly to signaling in the cochlea, but little is known about the effects of purinergic neurotransmission in the central auditory system. Here we examine P2 receptor-mediated signaling in the large spherical bushy cells (SBCs) of Mongolian gerbils around the onset of acous...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00186.2009

    authors: Milenkovic I,Rinke I,Witte M,Dietz B,Rübsamen R

    更新日期:2009-09-01 00:00:00

  • Human gaze stabilization during natural activities: translation, rotation, magnification, and target distance effects.

    abstract::Stability of images on the retina was determined in 14 normal humans in response to rotational and translational perturbations during self-generated pitch and yaw, standing, walking, and running on a treadmill. The effects on image stability of target distance, vision, and spectacle magnification were examined. During...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.4.2129

    authors: Crane BT,Demer JL

    更新日期:1997-10-01 00:00:00

  • Trial-by-trial discrimination of three enantiomer pairs by neural ensembles in mammalian olfactory bulb.

    abstract::Populations of output neurons in the mammalian olfactory bulb (OB) exhibit distinct, widespread spatial and temporal activation patterns when stimulated with odorants. However, questions remain as to how ensembles of mitral/tufted (M/T) neurons in the mammalian OB represent odorant information. In this report, the sin...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01334.2004

    authors: Lehmkuhle MJ,Normann RA,Maynard EM

    更新日期:2006-03-01 00:00:00

  • Rhythmically discharging basal forebrain units comprise cholinergic, GABAergic, and putative glutamatergic cells.

    abstract::The basal forebrain plays important roles in arousal, learning, and memory by stimulating cortical activation characterized by rhythmic slow theta and high-frequency beta-gamma activities. Although cholinergic neurons play a significant part in these roles, other, including GABAergic, neurons appear to contribute. Usi...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00938.2002

    authors: Manns ID,Alonso A,Jones BE

    更新日期:2003-02-01 00:00:00

  • Processing of interaural temporal disparities in the medial division of the ventral nucleus of the lateral lemniscus.

    abstract::The medial division of the ventral nucleus of the lateral lemniscus (VNLLm) contains a specialized population of neurons that is sensitive to interaural temporal disparities (ITDs), a potent cue for sound localization along the azimuth. Unlike many ITD-sensitive neurons elsewhere in the auditory system, neurons in the...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2002.88.2.666

    authors: Batra R,Fitzpatrick DC

    更新日期:2002-08-01 00:00:00

  • Decision making by urgency gating: theory and experimental support.

    abstract::It is often suggested that decisions are made when accumulated sensory information reaches a fixed accuracy criterion. This is supported by many studies showing a gradual build up of neural activity to a threshold. However, the proposal that this build up is caused by sensory accumulation is challenged by findings tha...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01071.2011

    authors: Thura D,Beauregard-Racine J,Fradet CW,Cisek P

    更新日期:2012-12-01 00:00:00

  • Varying the degree of single-whisker stimulation differentially affects phases of intrinsic signals in rat barrel cortex.

    abstract::Using intrinsic signal optical imaging (ISI), we have shown previously that the point spread of evoked activity in the rat barrel cortex in response to single-whisker stimulation encompasses a surprisingly large area. Given that our typical stimulation consists of five deflections at 5 Hz, the large area of evoked act...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1999.81.2.692

    authors: Polley DB,Chen-Bee CH,Frostig RD

    更新日期:1999-02-01 00:00:00

  • A color-based visualization technique for multielectrode spike trains.

    abstract::Multi electrode recordings of neuronal activity provide an overwhelming amount of data that is often difficult to analyze and interpret. Although various methods exist for treating multielectrode datasets quantitatively, there is a particularly prominent lack of techniques that enable a quick visual exploration of suc...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00758.2009

    authors: Jurjut OF,Nikolić D,Pipa G,Singer W,Metzler D,Mureşan RC

    更新日期:2009-12-01 00:00:00

  • Effect of eye rotation on visual-field map onto superior colliculus and visual cortex.

    abstract::We used multiunit recording to assess the effect of rotating one eye approximately 90 degrees at about the time of normal eye opening. Rotation of the eye did not alter the topography of the retinal maps onto visual cortex or superior colliculus. The intorted eye drove cells at most recording points in the contralater...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1983.50.3.618

    authors: Gordon B,Moran J,Presson J

    更新日期:1983-09-01 00:00:00

  • Multiple patterns and components of persistent inward current with serotonergic modulation in locomotor activity-related neurons in Cfos-EGFP mice.

    abstract::Using CFos-EGFP transgenic mice (P6-P12), we targeted persistent inward current (PIC) in the spinal interneurons activated by locomotion. Following a locomotor task, whole cell patch-clamp recordings were obtained from ventral EGFP+ neurons in spinal cord slices (200-250 μm from T₁₃-L₄). PIC was recorded by a family o...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01111.2009

    authors: Dai Y,Jordan LM

    更新日期:2010-04-01 00:00:00

  • Glutamate-induced long-term potentiation enhances spontaneous EPSC amplitude but not frequency.

    abstract::1. Many examples of long-term potentiation (LPT) are induced by repetitive electrical stimulation of presynaptic axons. LTP also is induced by direct glutamate iontophoresis (1 M, 1-2 microA, 10 s) onto postsynaptic neurons in hippocampal slices without evoked presynaptic stimulation; this form of LTP is called "ionto...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1996.75.5.1909

    authors: Cormier RJ,Kelly PT

    更新日期:1996-05-01 00:00:00

  • Visual activity in areas V3a and V3 during reversible inactivation of area V1 in the macaque monkey.

    abstract::1. Behavioral studies in the monkey and clinical studies in humans show that some visuomotor functions are spared in case of a V1 lesion. This residual vision appears to be subserved at least partially by visual activity in extrastriate cortex. Earlier studies have demonstrated that neurons in area V2 lose their visua...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1991.66.5.1493

    authors: Girard P,Salin PA,Bullier J

    更新日期:1991-11-01 00:00:00

  • Dynamics of reflex cocontraction in hermit crab abdomen: experiments and a systems model.

    abstract::1. Both stretch and release of the ventral superficial muscles (VSM) in the abdomen of the hermit crab, Pagurus pollicarus, activate the VSM motoneurons in the intact animal and in the isolated abdomen. 2. This reflex was studied by recording intracellularly from muscle fibers innervated by single motoneurons during s...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.69.6.1904

    authors: Chapple WD

    更新日期:1993-06-01 00:00:00

  • Inhibitory responses in Aplysia pleural sensory neurons act to block excitability, transmitter release, and PKC Apl II activation.

    abstract::Expression of the 5-HT(1Apl(a)) receptor in Aplysia pleural sensory neurons inhibited 5-HT-mediated translocation of the novel PKC Apl II in sensory neurons and prevented PKC-dependent synaptic facilitation at sensory to motoneuron synapses (Nagakura et al. 2010). We now demonstrate that the ability of inhibitory rece...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00767.2011

    authors: Dunn TW,Farah CA,Sossin WS

    更新日期:2012-01-01 00:00:00

  • Transient K current in the somatic membrane of cultured central neurons of embryonic rat.

    abstract::1. Somatic K currents of cultured hippocampal, striatal, and spinal cord neurons of embryonic rat were recorded under voltage clamp in membrane spheres ("blebs") excised by means of a tight-seal pipette. 2. The somatic K current in blebs was subject to rapid and near complete inactivation during 300-ms depolarizations...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.5.1708

    authors: Rizzo MA,Nonner W

    更新日期:1992-11-01 00:00:00

  • Rapid adaptation to Coriolis force perturbations of voluntary body sway.

    abstract::Studying adaptation to Coriolis perturbations of arm movements has advanced our understanding of motor control and learning. We have now applied this paradigm to two-dimensional postural sway. We measured how subjects (n = 8) standing at the center of a fully enclosed rotating room who made voluntary anterior-posterio...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00606.2018

    authors: Bakshi A,DiZio P,Lackner JR

    更新日期:2019-06-01 00:00:00

  • Sound-level-dependent representation of frequency modulations in human auditory cortex: a low-noise fMRI study.

    abstract::Recognition of sound patterns must be largely independent of level and of masking or jamming background sounds. Auditory patterns of relevance in numerous environmental sounds, species-specific vocalizations and speech are frequency modulations (FM). Level-dependent activation of the human auditory cortex (AC) in resp...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.00187.2001

    authors: Brechmann A,Baumgart F,Scheich H

    更新日期:2002-01-01 00:00:00

  • The Na+/Ca2+ exchanger inhibitor, KB-R7943, blocks a nonselective cation channel implicated in chemosensory transduction.

    abstract::The mechanism(s) of olfactory transduction in invertebrates remains to be fully understood. In lobster olfactory receptor neurons (ORNs), a nonselective sodium-gated cation (SGC) channel, a presumptive transient receptor potential (TRP)C channel homolog, plays a crucial role in olfactory transduction, at least in part...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90903.2008

    authors: Pezier A,Bobkov YV,Ache BW

    更新日期:2009-03-01 00:00:00

  • Sleep/wake firing patterns of human genioglossus motor units.

    abstract::Although studies of the principal tongue protrudor muscle genioglossus (GG) suggest that whole muscle GG electromyographic (EMG) activities are preserved in nonrapid eye movement (NREM) sleep, it is unclear what influence sleep exerts on individual GG motor unit (MU) activities. We characterized the firing patterns of...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00865.2007

    authors: Bailey EF,Fridel KW,Rice AD

    更新日期:2007-12-01 00:00:00