Abstract：
:DNA microarray is a technology that simultaneously evaluates quantitative measurements for the expression of thousands of genes. DNA microarrays have been used to assess gene expression between groups of cells of different organs or different populations. In order to understand the role and function of the genes, one needs the complete information about their mRNA transcripts and proteins. Unfortunately, exploring the protein functions is very difficult, due to their unique 3-dimentional complicated structure. To overcome this difficulty, one may concentrate on the mRNA molecules produced by the gene expression. In this paper, we describe some of the methods for preprocessing data for gene expression and for pairwise comparison from genomic experiments. Previous studies to assess the efficiency of different methods for pairwise comparisons have found little agreement in the lists of significant genes. Finally, we describe the procedures to control false discovery rates, sample size approach for these experiments, and available software for microarray data analysis. This paper is written for those professionals who are new in microarray data analysis for differential expression and want to have an overview of the specific steps or the different approaches for this sort of analysis.
journal_name
P R Health Sci Jjournal_title
Puerto Rico health sciences journalauthors
Suárez E,Burguete A,Mclachlan GJsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
89-104issue
2eissn
0738-0658issn
2373-6011journal_volume
28pub_type
杂志文章,评审abstract：:Although injection drug use (IDU) is the major risk category for HIV infection in Puerto Rico and injection drug users start their drug injection careers very young, there is little information related to drug injection patterns of young IDUs. In this paper we report on factors found to be associated with HIV seroposi...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Diagnosing cancer using microarray analysis to study differential gene expression has been a recent focus of intense research Although several very sophisticated analysis tools have been developed with this aim in mind, it still remains a challenge to keep these methods free of parametric adjustments as well as mainta...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2010-09-01 00:00:00
abstract：OBJECTIVES:The recent boom in patient education on chronic hepatitis C has resulted in a worldwide increase in the diagnosis of this condition. Available treatment is expensive and associated with significant side effects; therefore, many patients seek for alternative medicine. Silybum marianum is a natural herb known ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:Breast asymmetries have a high prevalence among women requesting breast augmentation. However, the prevalence of breast asymmetries in women undergoing other types of breast surgeries is not known. METHODS:The breast measurements of consecutively women evaluated for plastic surgery of the breast, but without...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2018-12-01 00:00:00
abstract：OBJECTIVE:To determine the IgE reactivity against recombinant protein Blo t 1 from the dust mite Blomia tropicalis (Bt) using serum from patients with positive skin test to this mite and to investigate the cross-reactivity between Bt and Dermatophagoides pteronyssinus. BACKGROUND:Dust mites have an important role as i...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2003-12-01 00:00:00
abstract：:The present study analyzed the socioeconomic determinants of health insurance status among Puerto Ricans. Given the fact that there are no mandated health benefits for private firms in Puerto Rico and that health services are provided almost free of charge in government facilities, Puerto Rico provides a unique case s...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1988-04-01 00:00:00
abstract：OBJECTIVE:To describe the respiratory pathogens found in the bronchoalveolar lavage of a Puerto Rican population infected with the human immunodeficiency virus (HIV). BACKGROUND:Empirical treatment is an accepted strategy for management of HIV-related pneumonia, but it is usually recommended for countries that have kn...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
abstract：:Endothelial cell proliferation and differentiation into blood capillaries (i.e., angiogenesis) are essential for growth and development, wound healing, osetogenesis, etc. But abnormal angiogenesis during tumor progression could lead to serious consequences. Angiogenesis is a complex biochemical process, and is often d...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1998-12-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of hypothyroidism in an adult female population in Puerto Rico and to determine the relationship between hypothyroidism, bone mineral density and vertebral and non-vertebral fractures in this population. METHODS:Data from the 400 subjects' database of the Latin American Vertebral ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:Patients hospitalized with unstable angina (UA) or with a non-ST segment elevation myocardial infarct (NSTEMI) are at increased risk of suffering refractory angina, recurrent myocardial infarct (MI), and death. These patients need to be evaluated more aggressively. According to the last published guidelines (2002) of ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2005-12-01 00:00:00
abstract：OBJECTIVE:This study aimed to assess the demographic, behavioral, and clinical factors associated with HIV and syphilis infection among a sample of men attending a sexually transmitted infection clinic during 2009 to 2010 in San Juan, Puerto Rico (PR). METHODS:A sample of 350 clinical records from men visiting the cli...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To determine the frequency of pediatric brain tumors treated at the University Pediatric Hospital of Puerto Rico over a 6-year period and examine the demographic data and tumor histology of patients in different age groups. METHODS:A retrospective study was undertaken at the University Pediatric Hospital. We...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:Exposure to lead in children living on a former landfill in Vega Baja-Puerto Rico, a United States Environmental Protection Agency (USEPA) designated Superfund Site, is a major health concern. Direct contact with lead-contaminated soil is considered a major exposure source. However, there is a lack of information rega...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：OBJECTIVE:Describe the pre-, intra-, and postoperative experience of colpocleisis in a group of patients at the University Hospital of the Puerto Rico Medical Center. METHODS:A chart review was done on a subgroup of patients, 70 years or older, on which colpocleisis was performed between January 2001 and December 2007...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:The World Health Organization criteria for the diagnosis of Osteopenia and Osteopoposis was applied to a control group of 33 females ages 50 to 59 years and 24 females ages 60 to 69 years. The general exclusion criteria for the selection of subjects included early menopause and diseases, use of drugs and toxic habits ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：:Although regeneration in the peripheral nervous system (PNS) after injury is robust, regeneration in the central nervous system (CNS) is abortive. The results from differences in the balance of regeneration inhibiting and promoting factors, which in the CNS is skewed toward inhibition while in the PNS it is skewed tow...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2000-09-01 00:00:00
abstract：:Rat gene 33 (g33) mRNA has a widespread tissue distribution. Insulin and various agents such as glucocorticoids, phorbol esters and plant lectins regulate G33 expression in rat hepatoma cells. The regulation of g33 by insulin and a phorbol ester was examined in two Chinese Hamster ovary (CHO) cell lines, CHO-T cells (...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:This study aimed to assess the role of gender on HIV/AIDS stigma among 507 medical students in Puerto Rico. METHODS:A secondary data analysis was performed with baseline measurements of a controlled randomized study. RESULTS:Unadjusted analyses showed that the overall multivariate test for gender was signif...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVE:This study intends to determine the prevalence of multidrug resistant (MDR) infections by A. baumannii, K. pneumoniae and P. aeruginosa in a tertiary care teaching hospital intensive care unit (ICU) in San Juan, PR, estimate the mortality rate and compare the morbidity and mortality differences among those tr...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2019-03-01 00:00:00
abstract：:This article examines the available data on the health status of Puerto Rican women in the United States. Statistics on reproductive health, cancer and AIDS indicate that the health status of these women is lower than that of both their U.S. and island counterparts. Usually, the health of immigrants lies somewhere bet...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:The therapy of chronic myeloid leukemia (CML), characterized by the presence of the Philadelphia chromosome in the clonal hematopoietic stem cells, has changed dramatically in the last year with the development of a specific inhibitor of the BCR-ABL tyrosine kinase. This medication, STI571 or imatinib, was recently ap...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：2002-09-01 00:00:00
abstract：:Seventy two partial subperiosteal implants were inserted in 52 patients. Thirty six of these implants had a classical design in which the submerged structure had thick primary and secondary connectors. Thirty six implants had a newly designed submerged structure consisting of long and thin primary connectors with groo...
journal_title：Puerto Rico health sciences journal
pub_type： 临床试验,杂志文章
doi：
更新日期：1994-09-01 00:00:00
abstract：:The prevalence of concomitant thyroid carcinoma with Grave's disease has been reported to range from 0 to 10%. Many controversies exist in the literature regarding the diagnostic workup and management in these types of patients. We are reporting a case of a 31 year old woman who had Graves' disease, a palpable thyroid...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：OBJECTIVE:to estimate the prevalence of pit and fissure sealants on first permanent molars in twelve year olds living in Puerto Rico and to further evaluate dental sealant prevalence by (1) urban/rural and public/private school status as well as (2) gender; DESIGN:population-based, cross-sectional study. SETTING:publ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Candida is the fourth most common cause of nosocomial bloodstream infections (BSI), being Candida albicans the most common species. This study evaluated the distribution of Candida spp isolates at a tertiary care medical center. The associated factors and outcome of patients with candidemia at the Puerto Rico Medical ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：:This study aims to determine the prevalence of Mycobacterium tuberculosis infection and its association with HIV and other health risk factors among drug users. A sample of 716 IDUs and crack users were enrolled from community sites. Consenting subjects were tested for HIV serum antibody status. Drug users with an unk...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:Thymidine kinase is a key enzyme responsible for the activation of several anticancer and antiviral drugs. As the first enzyme in the salvage pathway of thymidine, it is regulated by the feedback inhibition exerted by the end-product of the pathway, namely thymidine 5'-triphosphate. 5'-Aminothymidine is a non-toxic an...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：1994-03-01 00:00:00
abstract：:There has been a constant increase in the incidence of dengue in the Americas over the past 15 years. This has been caused by increased frequency of epidemic activity in most countries, as a result of increased numbers of virus serotypes circulating in the region. The change in disease ecology has resulted in the emer...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章,评审
doi：
更新日期：1987-08-01 00:00:00
abstract：:Formal training in public health in Puerto Rico began in 1940 when a degree-granting Division of Public Health was established within the existing School of Tropical Medicine. The division was sponsored by five institutions--the Puerto Rico Department of Health, the U.S. Public Health Service, the Children's Bureau, t...
journal_title：Puerto Rico health sciences journal
pub_type： 历史文章,杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Extracts from higher plants were evaluated for inhibitory activity against avian myeloblastosis virus reverse transcriptase. The assay may serve as a useful prescreen for potential anti-HIV bioactives. ...
journal_title：Puerto Rico health sciences journal
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
