IgE reactivity from serum of Blomia tropicalis allergic patients to the recombinant protein Blo t 1.

Abstract:

OBJECTIVE:To determine the IgE reactivity against recombinant protein Blo t 1 from the dust mite Blomia tropicalis (Bt) using serum from patients with positive skin test to this mite and to investigate the cross-reactivity between Bt and Dermatophagoides pteronyssinus. BACKGROUND:Dust mites have an important role as inducers of allergic asthma and rhinitis. Particularly, Bt is an important mite specie in the tropic and subtropical regions of the world. Therefore, recombinant allergens of this organism could be an important feature for development of effective diagnostic and therapeutic measures. MATERIALS AND METHODS:Purified recombinant Blot t 1 was produced in Escherichia coli and tested against sera from 54 allergic individuals by the dot blot technique. RESULTS:IgE response to Blot 1 was 72% for sera from patients with positive skin test to Bt. Cross-reactivity with Dermatophagoides pteronyssinus was not detected. Statistical analyses of the dot blot test results show 71.74% of sensitivity and 100% of specificity. CONCLUSION:By using a panel of allergic sera and an in vitro assay, the allergen rBlo t 1 exhibits no IgE cross-reactivity with Dermathophagoides pteronyssinus allergens. This finding suggests that specific clinical reagents are necessary for precise diagnosis and treatment of sensitization to Bt.

journal_name

P R Health Sci J

authors

Fonseca Fonseca L,Díaz AM

subject

Has Abstract

pub_date

2003-12-01 00:00:00

pages

353-7

issue

4

eissn

0738-0658

issn

2373-6011

journal_volume

22

pub_type

杂志文章
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    authors: Villegas VM,Díaz L,Emanuelli A,Izquierdo NJ,Townsend W

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    authors: Bansal N,Kumar PA,Agarwal MP,Aggarwal A

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    authors: Marrero C,Aponte A,Torres R,Santos F,Rivera J

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  • The Factors Significantly affecting the Quality of Life of Patients Who undergo Radical Cystectomy due to Bladder Cancer.

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    pub_type: 杂志文章

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    authors: Chabowski M,Juzwiszyn J,Malinowska K,Kostka A,Osmialowska E,Milan M,Malinowski M,Janczak D

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    authors: Banerjee DK

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    pub_type: 杂志文章,评审

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  • Reduction in the perinatal HIV transmission: the experience at the Maternal Infant Studies Center and Gamma Projects at the University of Puerto Rico School of Medicine.

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    pub_type: 杂志文章

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    journal_title:Puerto Rico health sciences journal

    pub_type: 杂志文章

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    更新日期:1997-09-01 00:00:00

  • The Zika Virus Infection in Pregnancy: Review and Implications for Research and Care of Women and Infants in Affected Areas.

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    pub_type: 杂志文章,评审

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    pub_type: 杂志文章,随机对照试验

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    pub_type: 杂志文章

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    pub_type: 临床试验,杂志文章,随机对照试验

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    authors: Adamsons K,de la Vega A,Santiago P

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    pub_type: 杂志文章

    doi:

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