Abstract：
BACKGROUND/AIM:Despite numerous studies, the etiology of chronic lymphocytic leukemia (CLL) remains unknown. A hypothesis of autoantigen stimulation in leukemic clone selection might explain 'stereotypy' of B-cell receptors. In healthy cells, cofilin-1 (CFL1) has multiple functions. Its role was described in several malignancies. The aim of this study was characterization of the role of CFL1 in CLL. Materialas and Methods: Cells from peripheral blood of 180 patients and 42 healthy volunteers (HVs) were isolated. Gene expression was assessed with reverse transcription polymerase chain reaction (RT-qPCR); western blot was performed for determination of protein level and activity. After silencing of CFL1 gene, cell ability for migration and chemotaxis was investigated with Transwell method. Post-silencing, apoptosis and cell cycle was determined by flow cytometry. RESULTS:In RT-qPCR, we observed significantly higher expression of CFL1. Higher activity of protein in CLL cells when compared to HVs was detected. Knock-down of CFL1 led to decreased chemotaxis and migration of CLL cells versus cells from HVs. Apoptosis was increased amongst cells with silenced CFL1 and correlated with higher proportion of cells in the G2/M phase. CONCLUSION:Significantly higher expression of CFL1 mRNA in CLL and higher protein activity might indicate high utilization of CFL1 in malignant cells, maintaining their viability, as its inhibition affected viability, cell-cycle progression and motility of leukemia cells.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Karp M,Karczmarczyk A,Bojarska-Junak A,Purkot J,Chojnacki M,ZajĄc M,Korpysz M,Tomczak W,Hus M,Morawska M,Giannopoulos Kdoi
10.21873/anticanres.14653subject
Has Abstractpub_date
2020-11-01 00:00:00pages
6327-6335issue
11eissn
0250-7005issn
1791-7530pii
40/11/6327journal_volume
40pub_type
杂志文章abstract：:Chk1 (checkpoint kinase 1) is a serine-threonine kinase that is critical for G2/M arrest in response to DNA damage. Chk1 phosphorylates Cdc25C at serine-216, a major regulatory site, in response to DNA damage. Furthermore, Chk1 also phosphorylates Cdc25A on serine 123 which accelerates its degradation through the ubiq...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-11-01 00:00:00
abstract：:Multidrug resistance (MDR) is a major clinical obstacle in the treatment of several cancers including hematological malignancies and solid tumors. The ATP-binding cassette transporter B1 (ABCB1) gene and its product, P-glycoprotein (P-gp), is one molecule that is involved in drug resistance. Here we report on the effe...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：BACKGROUND:Lymph node metastases from squamous cell carcinoma (SCC) can reach 20% in some high risk subgroups, and while early detection of metastasis may potentially improve overall and/or disease-specific survival, techniques for early detection have not been established. One such technique is the use of sentinel lym...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：2011-04-01 00:00:00
abstract：:We describe the case of an 80-year-old man with hepatocellular carcinoma (HCC) who developed tracheal obstruction due to peritracheal lymph node metastasis. A metastatic tumor that protruded into the airway was ablated using a neodymium yttrium-aluminium-garnet laser and then a self-expandable metallic stent (SEMS) wa...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 临床试验,杂志文章
doi：
更新日期：1993-03-01 00:00:00
abstract：:Almost all individuals with neurofibromatosis type 1 (NF1) develop peripheral nerve sheath tumors (PNSTs), mainly benign neurofibromas, however about 10% of PNSTs will undergo transformation to malignant peripheral nerve sheath tumors (MPNSTs). Surgical treatment of PNSTs has traditionally been regarded as a standard ...
journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：10.21873/anticanres.14136
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abstract：BACKGROUND:TZT-1027 (Soblidotin), a microtubule (MT)-depolymerizing agent, has antivascular activity through the disruption of microtubules in vascular endothelial cells. Our aim was to elucidate the mechanism of TZT-1027-induced antivascular activity by investigating the impact of various inhibitors. MATERIALS AND ME...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2007-11-01 00:00:00
abstract：BACKGROUND/AIM:Due to their unique composition of health-promoting compounds, the consumption of hazelnuts may contribute to the prevention of colon cancer. MATERIALS AND METHODS:Since hazelnuts are often consumed roasted, the impact of different roasting conditions (RC1=140.6°C/25 min, RC2=155.1°C/20 min and RC3=180....
journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.12195
更新日期：2018-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,评审
doi：
更新日期：1991-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：BACKGROUND:During the progression from low-grade dysplastic nodule (DN) to progressed hepatocellular carcinoma (HCC), intranodular portal tracts gradually disappear, while unpaired arteries develop increasingly. Contrast-enhanced ultrasound (CEUS) is highly accurate in depicting intranodular vascularity. This study eva...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-11-01 00:00:00
abstract：BACKGROUND:Incidence of brain metastases (BM) from breast cancer (BC) is increasing. However, prognostic evaluation and treatment strategies are still a matter of debate. AIM:To describe the clinical outcome of BM from BC treated by neurosurgical resection and to identify the actual prognostic factors in this specific...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：10.21873/anticanres.13208
更新日期：2019-02-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章,多中心研究
doi：
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:The proportion of geriatric gastric cancer (GC) patients with comorbidities has been increasing in Japan. This study was designed to evaluate the prognostic influence of the extent of lymph node dissection (END) and perioperative morbidities in patients with GC. PATIENTS AND METHODS:Between 2008 and 2013, t...
journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2016-04-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2011-10-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1992-01-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2012-11-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：BACKGROUND:Lenalidomide is an immunomodulatory drug frequently used for treatment of patients with multiple myeloma and myelodysplastic syndromes. This report presents a rare case of lenalidomide-associated hepatotoxicity and reviews the available literature. CASE REPORT:A 67-year-old male with multiple myeloma was ho...
journal_title：Anticancer research
pub_type： 杂志文章,评审
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更新日期：2012-09-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1999-05-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
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pub_type： 杂志文章
doi：10.21873/anticanres.12694
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journal_title：Anticancer research
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
