Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil.

Abstract:

:Extended-spectrum beta-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for bla SHV, bla TEM and bla CTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. Bla SHV, bla TEM and bla CTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment.

authors

Dropa M,Balsalobre LC,Lincopan N,Mamizuka EM,Murakami T,Cassettari VC,Franco F,Guida SM,Balabakis AJ,Passadore LF,Santos SR,Matté GR,Matté MH

doi

10.1590/s0036-46652009000400005

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

203-9

issue

4

eissn

0036-4665

issn

1678-9946

pii

S0036-46652009000400005

journal_volume

51

pub_type

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