Detection of citrate synthase autoantibodies in rats with chronic allograft nephropathy.


UNLABELLED:Citrate synthase (CS) is the one of the key enzymes in the citric acid cycle and an important mitochondrial autoantigen. The autoimmune responses against CS have not been studied in chronic allograft nephropathy (CAN). This study investigated the role of specific CS autoantibodies in rats bearing renal allografts affected with CAN. METHODS:Fisher344 rat renal grafts were orthotopically transplanted into Lewis rats following the procedure of Kamada with our modification. Lewis-to-Lewis and Fisher344-to-Fisher344 kidney transplantations were also performed as autologous control groups (each n = 9). All the allograft recipients given cyclosporine (10 mg/kg(-1)d(-1) x 10 d) were divided into four groups (each n = 9): (1) vehicle: normal saline orally; (2) cyclosporine: 6 mg/kg(-1)d(-1); (3)FK506: 0.15 mg/kg(-1)d(-1); (4) mycophenolate mofetil (MMF): 20 mg/ kg(-1)d(-1). At 4, 8, and 12 weeks posttransplantation, the animals were sacrificed to harvest sera and renal allografts. The serum creatinine (SCr) was measured and pathological changes assessed according to Banff 97 criteria. IgM and IgG isotypes of CS antibodies were detected in all recipient sera by enzyme linked immunosorbent assays. RESULTS:Both IgM and IgG isotype CS autoantibodies were observed in the sera of all the recipients before and after transplantation, but the levels of IgM CS autoantibody were obviously higher than IgG isotype in all the blood samples. It was stable not only in autologous but also in allograft groups. In both autologous groups, the SCr and IgM and IgG isotype CS autoantibodies showed no obvious change before and after transplantation, and no typical CAN occurred. The values of IgG isotype of CS autoantibody (DeltaOD) at 4, 8 and 12 weeks were stable. At 4 weeks, the values of SCr, Banff score, and IgG isotype CS autoantibody (DeltaOD) were not significantly different (P > .05) among the allograft groups. At 8 and 12 weeks, with progression of CAN in vehicle, cyclosporine and FK506 groups' values of SCr, Banff score, and IgG (DeltaOD) also increased dramatically (P = .005) in all three groups when compared with the baseline and 4 week values, but the differences among the three groups were not significant (P > .05). At 8 and 12 weeks, the MMF group suffered mild-to-moderate CAN, but the values of SCr and Banff score were significantly lower than those in the other three groups. MMF significantly inhibited the formation of IgG (DeltaOD) when compared with the other three groups (P = .02). CONCLUSION:This study suggested that the IgG isotype of CS autoantibody contributes to CAN after kidney transplantation. The IgM isotype is physiological. MMF significantly inhibited the formation of IgG isotype CS autoantibody, which may be related to its effects to alleviate CAN.


Transplant Proc


Zhang LY,Lu YP,Yang L,Luo GH,Song J,Li YP




Has Abstract


2009-12-01 00:00:00














  • Liver transplantation in pigs with small-for-size grafts: effect of portocaval shunt.

    abstract::Small-for-size livers are associated with graft dysfunction, probably due to portal hyperperfusion. Modulation of the recipient portal inflow is a new option in these cases. This article sought to analyze the effect of portocaval shunt in small-for-size liver grafts in pigs. Twelve liver transplants were performed in ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Reichert PR,Pires JS,Sallet CA,Brun MV,Vesco JD,Bonadeo NM,Missel J,Sossela R,Rodrigues R,Fracasso JI,Malheiros AV,Bajerski JC,Annes RD,Guimarães TT,Santos FC,Ben AJ,Gressler M,Wolff NM,Winkelmann G,Manzatto LB,Sa

    更新日期:2004-05-01 00:00:00

  • Tubular CD44 expression in renal allograft biopsies.

    abstract::CD44 is a type I transmembrane glycoprotein serving as a cell adhesion receptor, whose main ligand is hyaluronic acid, but also may interact with collagen, laminin, fibronectin, and osteopontin. This marker is involved in cell migration, homing, activation, metastasis, and inflammation. Tubular CD44 expression has bee...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sarioğlu S,Sis B,Tuncer C,Celik A,Zeybel M,Soylu A,Gülay H

    更新日期:2004-01-01 00:00:00

  • Frequency of Human Papilloma Virus Occurrence Among Pathological Changes of the Oral Cavity in Kidney Allotransplant Recipients Undergoing Long-Term Pharmacological Immunosuppressive Therapy.

    abstract::Recipients of allotransplants are more susceptible to viral infections, among which the human papilloma virus infection is an independent factor inducing precancerous lesions and cancers of both the anogenital and the cervicocephalic region. MATERIALS AND METHODS:The study included a group of 69 allogenic kidney tran...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Szubińska-Lelonkiewicz D,Osiak M,Wychowański P,Siewert-Gutowska M,Fiedor P

    更新日期:2018-01-01 00:00:00

  • Neutrophilic reversible airways dysfunction after liver transplantation: a case report.

    abstract::In the present case report we have described a 46-year-old female patient who underwent a liver transplantation in 1998 for polycystic disease and developed a syndrome of increasing dyspnea, with sputum production and a progressive decline in pulmonary function [forced expiratory volume in one second (FEV(1)) (decreas...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Verleden GM,Vos R,De Vleeschauwer S,Verleden S,Dupont L,Nevens F,Verbeken E,Vanaudenaerde BM

    更新日期:2011-06-01 00:00:00

  • Experience with cyclosporine: from revolution to evolution of immunosuppressive protocols in thoracic organ transplantation.

    abstract::The introduction of cyclosporine into clinical practice of thoracic organ transplantation had a dramatic and positive effect on both short- and long-term survival. Today, the majority of patients are still treated with this drug, and different immunosuppressive combination therapies have further resulted in improved l...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Haverich A,Gorler H

    更新日期:2004-03-01 00:00:00

  • Basiliximab: efficacy and tolerability in adults and children.

    abstract:UNLABELLED:An open, single arm, prospective clinical trial to assess the efficacy and safety of basiliximab (Simulect) combined with cyclosporine microemulsion (Neoral), steroids, and azathioprine was performed in four centers in Chile, two adult and two pediatric. The 23 patients who were enrolled were followed for 12...

    journal_title:Transplantation proceedings

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Mocarquer A,Pinto V,Buckel E,Lagos E,Pefaur J,Ramirez K,Morales J,Rosatti P,Elberg A,Simulect Multicenter Study Group of Chile.

    更新日期:2003-11-01 00:00:00

  • A 6-month, open-label, multicenter clinical study in Korean de novo renal transplant patients evaluating the efficacy, safety, and tolerance of myfortic concomitantly used with tacrolimus.

    abstract::Enteric-coated mycophenolate sodium (myfortic, Novartis Pharma AG, Basel, Switzerland) is designed to improve the gastrointestinal tolerability of micophenolic acid. This study was designed to evaluate the efficacy and safety of myfortic in Korean de novo renal transplantation. A total of 65 patients from four transpl...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,多中心研究


    authors: Ju MK,Park SH,Kim SJ,Moon IS,Kim YS

    更新日期:2012-01-01 00:00:00

  • Real-time monitoring of nitric oxide (NO) and pO2 levels under ischemic conditions associated with small bowel ischemia/reperfusion injury using selective electrodes for NO and oxygen molecules.

    abstract::The present study demonstrated the feasibility of monitoring nitric oxide (NO) and pO2 levels under ischemic conditions associated with small bowel ischemia/reperfusion (I/R) injury through the use of selective electrodes for NO and oxygen molecules. NO levels gradually increased during ischemia. When reperfusion was ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Watanabe T,Owada S,Kobayashi H,Ishiuchi A,Nakano H,Asakuta T,Shimamura T,Asano T,Koizumi S,Jinnouchi Y,Katayama M,Kamibayasi M,Murakami E,Otsubo T

    更新日期:2007-12-01 00:00:00

  • Role of N-acetylcysteine on renal function in patients after orthotopic heart transplantation undergoing coronary angiography.

    abstract:INTRODUCTION:The aim of this study was to assess nephroprotective influence of intravenous N-acetylcysteine (NAC) on renal function after radiocontrast use in calcineurin inhibitor-treated patients after orthotopic heart transplantation (OHT). MATERIALS AND METHODS:We analyzed the results of 112 consecutive coronary a...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,随机对照试验


    authors: Barańska-Kosakowska A,Zakliczyński M,Przybylski R,Zembala M

    更新日期:2007-11-01 00:00:00

  • Comparison of QuantiFERON-TB Gold with tuberculin skin test for detection of latent tuberculosis infection before kidney transplantation.

    abstract:INTRODUCTION:Screening for latent tuberculosis infection (LTBI) before kidney transplantation (KT) is an indispensable process, purposes of this study were to compare the QuantiFERON-TB Gold In-Tube test (QFT-GIT) with the tuberculin skin test (TST) for screening of LTBI in kidney transplant recipients (KTRs). METHODS...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Kim JS,Cho JH,Park GY,Kang YJ,Kwon O,Choi JY,Park SH,Kim YL,Kim HK,Huh S,Kim CD

    更新日期:2013-10-01 00:00:00

  • The role of dendritic cells in the gastrointestinal field effect.

    abstract:INTRODUCTION:Intestinal manipulation leads to local bowel wall inflammation that subsequently spreads over the entire gastrointestinal tract. Previously, this gastrointestinal field effect had been demonstrated by us in a rodent model. We herein postulated an immunologic mechanism mediated by activated leukocytes. The ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Koscielny A,Boerner T,Wehner S,Kurts C,Kalff JC

    更新日期:2006-07-01 00:00:00

  • Thromboelastography as a Predictor of Outcomes Following Liver Transplantation.

    abstract:BACKGROUND:Thromboelastography (TEG) has been used perioperatively during liver transplantation (LT) to provide a real-time global hemostasis assessment for targeted blood product replacement. We aimed to analyze the relationship between post-LT TEG results and outcomes. METHODS:We retrospectively analyzed patients un...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Trautman CL,Palmer WC,Taner CB,Canabal JM,Getz T,Goldman A,Heckman MG,Diehl NN,Lee DD,Stancampiano FF

    更新日期:2017-11-01 00:00:00

  • Intrahepatic Microdialysis for Monitoring of Metabolic Markers to Detect Rejection Early After Liver Transplantation.

    abstract:OBJECTIVES:The clinical and biochemical manifestations of acute rejection after liver transplantation are nonspecific, and a liver biopsy is often needed to verify the diagnosis. This may delay treatment. The aim of this study was to evaluate whether monitoring of intrahepatic glucose, lactate, pyruvate, and glycerol b...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: von Platen A,D'Souza MA,Rooyackers O,Nowak G

    更新日期:2021-01-01 00:00:00

  • Use of intraperitoneal ePTFE Gore dual-mesh plus in a giant incisional hernia after kidney transplantation: a case report.

    abstract::We evaluated the incidence of and predisposing factors for an incisional hernia after kidney transplantation. Numerous techniques have been used to repair postoperative fascial dehiscences or simple incisional hernias, but no clear treatment exists for giant hernias. Our aim was to obtain (1) a safe procedure to repai...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Lo Monte AI,Damiano G,Maione C,Gioviale MC,Lombardo C,Buscemi G,Romano M

    更新日期:2009-05-01 00:00:00

  • Metaanalysis of statins and survival in de novo cardiac transplantation.

    abstract:BACKGROUND:The use of HMG CoA reductase inhibitors (statins) after cardiac transplantation has been suggested to decrease the incidence of severe rejection and improve survival. Individual investigations that have led to this suggestion are randomized (but not placebo-controlled) studies, including small patient number...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,meta分析


    authors: Mehra MR,Raval NY

    更新日期:2004-06-01 00:00:00

  • Current concepts of autoimmune disease.

    abstract::Our concepts of autoimmune disease have evolved with our changing understanding of the immune response. In recent years, clonal deletion theories have gradually given way to more dynamic views of the regulation of anti-self immune reactions. It is now clear that self-reactive B cells and some self-reactive T cells per...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章,评审


    authors: Rose NR

    更新日期:1988-06-01 00:00:00

  • Effects of tolerance induction on the actions of interferon-gamma on porcine cardiac allografts.

    abstract::It is well known that interferon-gamma (IFN-gamma) not only plays a critical role in antigen-dependent but also in antigen-independent tissue injury; however, it is not clear how tolerance induction affects the actions of IFN-gamma in the transplant setting. To address this question, we compared the effects of IFN-gam...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Hoerbelt R,Benjamin LC,Shoji T,Johnston DR,Muniappan A,Guenther DA,Allan JS,Houser SL,Madsen JC

    更新日期:2006-12-01 00:00:00

  • Changes in Tumor Markers and Their Implications in Selecting Liver Transplantation for Patients With Hepatocellular Carcinoma.

    abstract:BACKGROUND AND AIM:It has been suggested that tumor markers can provide additional information over tumor size and number-based liver transplantation (LT) criteria. We aimed to assess if changes in tumor markers are associated with the risk of tumor recurrence after LT for hepatocellular carcinoma (HCC) who received lo...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Song BG,Sinn DH,Kang W,Gwak GY,Paik YH,Choi MS,Lee JH,Koh KC,Paik SW,Kim JM,Joh JW,Choi GS

    更新日期:2020-04-01 00:00:00

  • Causes for an Incomplete Donation Process at a Tertiary Care Hospital.

    abstract:INTRODUCTION:This study aims to identify the causes for the incomplete donation process at a tertiary care hospital. MATERIALS AND METHODS:A descriptive, retrospective study was performed; all potential donors reported to the Transplant Service within the period of 2005 to 2014 were included. Descriptive statistics we...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Guerra-Sáenz EK,Narváez-Navarro AG,Hernández-López AC,Rojas-Delgado CE,Ruíz-Cantú G,Cordero-Pérez P,Hernández-Guedea M,Zapata-Chavira H,Escobedo-Villarreal MM,Muñoz-Espinosa L,Pérez Rodríguez E

    更新日期:2016-03-01 00:00:00

  • Update on Causes of Family Refusal for Organ Donation and the Related Factors: Reporting the Changes Over 6 Years.

    abstract:INTRODUCTION:After significant improvement of the family consent rate to organ donation (OD) in recent years, owing to an increase in cultural activities and social awareness, a plateau has been reached. This study was performed to detect the causes for this plateau. METHODS:We reviewed exact causes of family refusal ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Mojtabaee M,Ghorbani F,Mohsenzadeh M,Beigee FS

    更新日期:2018-01-01 00:00:00

  • Curcumin Attenuates Hemorrhagic Shock and Blood Replenish Resuscitation-induced Impairment of Pulmonary Barrier Function by Increasing SIRT1 and Reducing Malondialdehyde and TNF-α Contents and Neutrophil Infiltration in Lung in a Dose-Dependent Fashion.

    abstract:OBJECTIVES:Acute lung injury (ALI) is a critical complication subsequent to hemorrhage shock and resuscitation (HSR) that frequently leads to multiple organ failure. Collective evidence suggested that the activation of pulmonary nicotinamide adenine dinucleotide-dependent deacetylase sirtuin-1 (SIRT1) plays a critical ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Yu-Wung Yeh D,Wang JJ

    更新日期:2020-01-01 00:00:00

  • Comparative study between living and cadaveric donors in pediatric liver transplantation.

    abstract:UNLABELLED:We examined whether the results in living-related hepatic transplantation (LRLT) are better than those from a cadaveric donor (CDLT). MATERIAL AND METHODS:The last 27 consecutive LRLT, performed from 1998 to 2005, were compared with 27 CDLT matched for age, weight, date, and diagnosis. Grafts in LRLT group ...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Oliveros FH,Santamaría ML,Gámez M,Murcia J,Leal N,Frauca E,Hierro L,Camarena C,de la Vega A,Bortolo G,Díaz MC,Jara P

    更新日期:2005-11-01 00:00:00

  • The effect of anti-human leukocyte antigen, anti-major histocompatibility complex class 1 chain-related antigen a, and anti-glutathione transferase-T1 antibodies on the long-term survival of renal allograft.

    abstract:BACKGROUND:One of the most important mechanisms of allograft rejection is the production of donor-specific antibodies (DSA). Anti-major histocompatibility complex class-I chain-related antigen A (MICA) and anti-glutathione S transferase-T1 (GSTT1) antibodies cause graft dysfunction and reduce graft survival. The aim of...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Akgul SU,Oğuz FS,Çalışkan Y,Kekik C,Çağatay P,Türkmen A,Nane I,Aydın F,Temurhan S

    更新日期:2013-04-01 00:00:00

  • The practical, moral, and ethical considerations of the new Israeli law for the allocation of donor organs.

    abstract::There is a worldwide shortage of organs for transplantation; the number of patients on the waiting list exceeds the supply of available organs. The demand for organ donation is particularly concerning in Israel. A new Israeli law has been instituted to give those who sign donor cards allocation priority if they are ev...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Gruenbaum BF,Jotkowitz A

    更新日期:2010-12-01 00:00:00

  • Cytomegalovirus infections in cardiac transplant patients: an experience at a clinical hospital, university of Chile.

    abstract:BACKGROUND:Since cytomegalovirus (CMV) infects between 20% and 50% of heart transplant patients, we reviewed our experience in 7 cases of this infection. METHODS:A prospective analysis of CMV infection was performed in heart transplant patients who received cyclosporine, azathioprine, or mycophenolate mofetil, and pre...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sepulveda L,Llancaqueo M,Zamorano J,Bermudez C,Cortes C

    更新日期:2007-04-01 00:00:00

  • Evaluation of the efficacy and safety of the conversion from a calcineurin inhibitor to an everolimus-based therapy in maintenance renal transplant patients.

    abstract::Everolimus has recently been introduced into clinical practice with promising perspectives due to its efficacy, lack of nephrotoxicity, and antitumor effects. Experience in clinical trials associated with low-dose cyclosporine showed good results, but there is almost no experience in calcineurin inhibitor (CNI) elimin...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Sánchez Fructuoso A,Ruiz San Millán JC,Calvo N,Rodrigo E,Moreno MA,Cotorruelo J,Conesa J,Gómez-Alamillo C,Arias M,Barrientos A

    更新日期:2007-09-01 00:00:00

  • Italian continuing medical education program: an evidence-based course for surgeons and health personnel of a regional transplantation center.

    abstract::In the organ transplantation area the application of the evidence-based medicine (EBM) methods may be limited by several, heterogeneous conditions, eg, mandatory laws and protocols, logistic concerns, as well as donor/recipient matching. In this report we have described the results of a first EBM-oriented course for s...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Santori G,Renzini AM,Lasagna F,Casaccia M,Fontana I,Valente U

    更新日期:2011-05-01 00:00:00

  • Improvement of mortality rate and decrease in histologic hepatic injury after human cord blood stem cell infusion in a murine model of hepatotoxicity.

    abstract:BACKGROUND AND AIMS:Because of their plasticity potential local and systemic application of cord blood stem cells may represent excellent candidates for cell-based therapeutic strategies in toxic liver injuries. It is already known that intraperitoneal administration of hematopoietic stem cells provides rapid liver hom...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Di Campli C,Piscaglia AC,Rutella S,Bonanno G,Vecchio FM,Zocco MA,Monego G,Michetti F,Mancuso S,Pola P,Leone G,Gasbarrini G,Gasbarrini A

    更新日期:2005-07-01 00:00:00

  • Correlation of C0 and C2 levels with cyclosporine side effects in kidney transplantation.

    abstract::Cyclosporine has a narrow therapeutic window requiring close monitoring to ensure adequate immunosuppression while avoiding nephrotoxicity and other side effects. Pharmacokinetic studies have suggested that cyclosporine levels at 2 hours postdose (C2) is the best single time point to predict area under the concentrati...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Rodrigo E,Ruiz JC,Angeles de Cos M,Ruiz J,Gago M,Piñera C,Sánchez B,González-Cotorruelo J,Gómez-Alamillo C,Arias M

    更新日期:2009-07-01 00:00:00

  • Accurate method for preoperative estimation of the right graft volume in adult-to-adult living donor liver transplantation.

    abstract:BACKGROUND:Accurate estimation of graft volume is crucial to avoid small-for-size syndrome following adult-to-adult living donor liver transplantation AALDLT). Herein, we combined radiological and mathematical approaches for preoperative assessment of right graft volume. METHODS:The right graft volume was preoperative...

    journal_title:Transplantation proceedings

    pub_type: 杂志文章


    authors: Khalaf H,Shoukri M,Al-Kadhi Y,Neimatallah M,Al-Sebayel M

    更新日期:2007-06-01 00:00:00