Abstract：
:The development of a small animal model for hepatitis C virus (HCV) infection is a critical issue for the development of novel anti-HCV drugs. To this aim, we have tried many different approaches for generating mice carrying humanized liver. Main efforts were focused on the transplantation of human hepatocytes into immunocompromised mice (SCID-/-, Bg-/-) carrying a genetic lethal liver disease (Alb-uPA). Survival of homozygotic animals should largely depend on early transplantation with healthy hepatocytes. In parallel to establishing a colony of Alb-uPA/SCID/Bg mice, we developed a microsurgical procedure for intrasplenic xenotransplantation of healthy hepatocytes in 1-week-old mice. So far, we generated several chimeras by xenotransplanting human hepatocytes in Alb-uPA+/+/SCID-/-/Bg-/- mice at 1 week after birth. In a first step, identification of successfully engrafted animals is possible by quantification of human serum albumin and human alpha 1 antitrypsin in mouse sera. Additional preliminary histomorphological analysis of liver sections from chimeric animals was also carried out. One of the mice was transiently infected with HCV, reaching viremia levels of approximately 10(5) genomes/mL. However, the efficiency of this system to generate chimeric mice is still very limited. We are currently exploring the use of more robust models of hepatic disease. Moreover, we have been also exploring novel strategies for the generation of chimeric mice by xenotransplanting human adult stem cells, instead of human hepatocytes, at preimmune stages of development.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Turrini P,Sasso R,Germoni S,Marcucci I,Celluci A,Di Marco A,Marra E,Paonessa G,Eutropi A,Laufer R,Migliaccio G,Padron Jdoi
10.1016/j.transproceed.2006.02.149subject
Has Abstractpub_date
2006-05-01 00:00:00pages
1181-4issue
4eissn
0041-1345issn
1873-2623pii
S0041-1345(06)00258-2journal_volume
38pub_type
杂志文章abstract：BACKGROUND:It has become common practice to withdraw or reduce calcineurin inhibitors (CNI) in patients with renal dysfunction after liver transplantation; however, little is known about the long-term outcome of this strategy. This study investigates the long-term results of CNI withdrawal for post-liver transplant ren...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.10.044
更新日期：2011-12-01 00:00:00
abstract：:Several groups have performed graft biopsies at set times posttransplant ("protocol biopsies") and found unequivocal histologic criteria for acute rejection in a high proportion of patients with stable graft function. The significance of "subclinical" rejection remains controversial. Our group and others have shown th...
journal_title：Transplantation proceedings
pub_type： 杂志文章,评审
doi：10.1016/j.transproceed.2003.12.051
更新日期：2004-03-01 00:00:00
abstract：:The aim of this study was to compare incidence rates of mechanical and infectious complications associated with central venous catheterization via the internal jugular vein (IJV) versus the subclavian vein (SV) among 45 consecutive patients undergoing orthotopic liver transplantation (OLT) between January 2000 and Jun...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章
doi：10.1016/j.transproceed.2005.07.021
更新日期：2005-09-01 00:00:00
abstract：:Porcine neonatal pancreatic cell clusters (NPCCs) isolated from 1- to 3-day-old pigs cured diabetic nude mice more than 14 weeks after transplantation. To shorten the latent period between transplantation and reversal of hyperglycemia, we investigated the effects of insulin-like growth factor-1 (IGF-1) and NPCCs isola...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.03.057
更新日期：2009-06-01 00:00:00
abstract：:Portopulmonary hypertension (PoPH) is a common and feared complication of end-stage liver disease, and imposes increased risk of perioperative morbidity in the liver transplant patient. Herein, we present the first successful use of Imatinib in the perioperative management of a patient who was not responding to conven...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.02.100
更新日期：2009-06-01 00:00:00
abstract：INTRODUCTION:Renal transplantation (RT) in patients infected with human immunodeficiency virus (HIV) has significantly improved under the advent of combined antiretroviral therapy (cART). The authors describe their experience in RT in patients with HIV from September 2010 to June 2013. CASES REPORT:Four patients under...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2014.05.030
更新日期：2014-07-01 00:00:00
abstract：OBJECTIVE:Cytomegalovirus (CMV) is the most common viral infection after allotransplantation; it can be a major cause of morbidity and mortality. Our aim was to analyze the main risk factors that lead to development of CMV infection and disease. PATIENTS AND METHODS:We retrospectively analyzed 207 patients who receive...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.06.057
更新日期：2009-07-01 00:00:00
abstract：:Cyclosporine absorption has been key to obtaining adequate results in immunosuppressive regimens. Since 2005, we have used a different monitoring program for Cyclosporine among pediatric liver transplant recipients namely, two hours post dosing concentrations (O(2)). This study of 128 patients included 50.8% males and...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.11.016
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND:This study sought to establish an actual risk-based long-term screening protocol for hepatocellular carcinoma (HCC) recurrence after liver transplantation (OLT). METHODS:The study was a retrospective review of medical records from 334 HCC patients who underwent primary living donor OLT and followed up for a...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.08.068
更新日期：2013-10-01 00:00:00
abstract：:The results from previous trials suggested that tacrolimus-based treatment in kidney transplantation was associated with a significantly lower incidence of acute rejection, and that cyclosporine microemulsion (CsA-Me)-treated patients converted to tacrolimus had numerically better 6-year graft survival than those rema...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.11.081
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:Proteinuria in the nontransplant population is a progressive renal disease. We analyzed the prevalence and clinical significance of proteinuria as well as factors related to its degree at posttransplant year 1 among kidney transplant recipients. METHODS:We measured protein in a 24-hour urine among 644 recipien...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2011.11.060
更新日期：2012-04-01 00:00:00
abstract：:Perfusion parameters (PP) and early transplant outcome data from 332 consecutive ECD type kidneys machine preserved on the Waters RM-3 apparatus were reviewed and analyzed to examine the validity of using suboptimal PPs (renal resistance of .41-.60) as a criterion for discarding kidneys. Overall discard rate was 23.5%...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2005.01.058
更新日期：2005-01-01 00:00:00
abstract：OBJECTIVE:To develop a radial basis function (RBF) neural network and investigate its performance in the estimation of glomerular filtration rate (GFR) for patients with chronic kidney disease. METHODS:A total of 651 patients with chronic kidney disease were enrolled in this study. The GFR measured by 99mTc-DTPA renal...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2020.01.008
更新日期：2020-04-01 00:00:00
abstract：:Adult respiratory distress syndrome is a severe respiratory failure due to noncardiogenic pulmonary edema with high mortality rates (50-70%). The most common etiology of respiratory distress is sepsis, but it may also be caused by several of the immunosuppressants prescribed in transplantation. In the last year, influ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2013.06.015
更新日期：2013-11-01 00:00:00
abstract：:Extreme myocardial degeneration leading to advanced stages of cardiomyopathy with extensive atrophy is rarely observed before patients die. However, heterotopic transplantation is a special situation wherein this phenomenon can be observed. The greater part of the failed heart shows recuperation after receiving circul...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.02.012
更新日期：2009-04-01 00:00:00
abstract：:Pancreas transplantation is currently the only known therapy to restore glycemic metabolism in type 1 diabetic patients. Its most prevalent indication is in association with kidney transplantation (simultaneous pancreas and kidney transplantation SPK) for patients with type 1 diabetes mellitus (DM1) and nephropathy, w...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.02.020
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Liver transplantation is the criterion standard treatment for hepatocellular carcinoma, but tumor recurrence remains a problem leading to a worse prognosis. We aimed to evaluate factors associated with recurrence of hepatocellular carcinoma and survival after this procedure. METHODS:We retrospectively revie...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2016.02.063
更新日期：2016-07-01 00:00:00
abstract：:Since November 1974, heterotopic cardiac transplants have been performed at this unit in preference to orthotopic transplants. A number of reasons have led us to prefer this technique, namely donor malfunction in the immediate postoperative period and during rejection; right ventricular failure of the donor heart in t...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：BACKGROUND:Traditional open donor nephrectomy (ODN) is associated with good outcomes and excellent allograft function; nevertheless, it causes too much injury to the donor. Hand-assisted laparoscopic donor nephrectomy (HLDN) may accomplish these goals with less damage. We compared the outcomes of the hand-assisted with...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.05.081
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:Bronchiolitis obliterans is the most significant complication adversely affecting the survival of lung allograft recipients. Injury and loss of epithelium are associated with obliteration of the airway lumen. The aim of this study was to examine the effects of various immunosuppressants on airway epithelium....
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2006.01.042
更新日期：2006-04-01 00:00:00
abstract：BACKGROUND:This study was performed to determine the factors that cause arterial hypertension after heart transplantation (HT) and the drugs used in its management. MATERIALS AND METHODS:We studied 247 consecutive patients who had undergone HT between 2000 and 2006 and who survived for at least 6 months. We excluded p...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.08.112
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:Acute cellular rejection in heart transplants is characterized by an active lymphocytic infiltration, whereas the humoral response shows complement deposits in myocardial tissue. Both reactions may produce hemodynamic compromise during the first months after orthotopic heart transplantation (OHT). The aim of ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.08.028
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:Machine perfusion preservation has been used experimentally to extend the storage interval of donor hearts. We previously demonstrated that machine perfusion with glucose-supplemented Celsior preservation solution led to superior reperfusion function but resulted in increased myocardial edema compared with co...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2010.02.073
更新日期：2010-06-01 00:00:00
abstract：:The aim of this study was to document, in hyperhomocysteinemic renal transplant recipients, the effect of vitamin supplementation on carotid intima-media thickness (cIMT). Fifty-six hyperhomocysteinemic stable renal transplant recipients were randomly assigned to either vitamin supplementation (group A) or placebo tre...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.transproceed.2005.06.101
更新日期：2005-07-01 00:00:00
abstract：:Cyclosporine has a narrow therapeutic window requiring close monitoring to ensure adequate immunosuppression while avoiding nephrotoxicity and other side effects. Pharmacokinetic studies have suggested that cyclosporine levels at 2 hours postdose (C2) is the best single time point to predict area under the concentrati...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2009.06.155
更新日期：2009-07-01 00:00:00
abstract：BACKGROUND:Converting to once-daily tacrolimus (Advagraf [Adv]) among renal transplant patients results in better drug adherence. Data regarding dosage and intrapatient variability changes after conversion among patients with CYP3A4/5 inhibitors (CYPinh) is lacking. METHOD:A retrospective chart review among all kidney...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2019.02.065
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND:Urinary tract infections (UTIs) are the most common infectious complications among kidney recipients. They occur due to the lack of an effective antireflux mechanism in the majority of techniques used during kidney transplantation. Subureteral injection of tissue bulking substance is a feasible alternative t...
journal_title：Transplantation proceedings
pub_type： 临床试验,杂志文章
doi：10.1016/j.transproceed.2020.01.104
更新日期：2020-10-01 00:00:00
abstract：UNLABELLED:Organ transplantation as a substitute for diseased organs in end-stage organ failure has led to a worldwide increase in this treatment modality, but donation has not kept pace with the demand, despite scientific, social, and government efforts. For many years, Hispanic donation in Puerto Rico was meager and ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2008.03.120
更新日期：2008-05-01 00:00:00
abstract：BACKGROUND:The pathophysiology of Crohn's disease (CD) is related to immune dysregulation making it unique among indications for intestinal transplants (ITx). We examined whether outcomes of ITx for CD are any worse than the overall ITx population. METHODS:United Network for Organ Sharing Standard Transplant Analysis ...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.04.030
更新日期：2013-11-01 00:00:00
abstract：:To show drug interactions between tacrolimus and ertapenem, we retrospectively evaluated 13 renal transplant recipients who had been treated with ertapenem for urinary tract infections during prescription of a constant dose. The mean dose of tacrolimus to achieve desired therapeutic concentrations decreased significan...
journal_title：Transplantation proceedings
pub_type： 杂志文章
doi：10.1016/j.transproceed.2012.08.003
更新日期：2012-12-01 00:00:00
