Differentiation of variola, monkeypox, and vaccinia antisera by radioimmunoassay.

Abstract:

:Poxvirus antisera adsorbed with "homologous" and "heterologous" poxvirus-infected chorioallantoic membranes (CAM) were differentiated by solid-phase radioimmunoassay (RIA). Mixtures of the antiserum dilutions and infected CAM were added directly (without centrifugation) to poxvirus-infected CAM antigens affixed to wells of microtitration plates. The affixed antigens combined with unadsorbed antibodies, and the cross-reactive antigen-antibody complexes were removed by washing. The results showed that adsorption of an antiserum with variola-, vaccinia-, or uninfected-CAM antigen and subsequent reaction of each in RIA with monkeypox- and uninfected-CAM antigens allowed the identification of antivariola, antivaccinia, or antimonkeypox sera.

authors

Hutchinson HD,Ziegler DW,Wells DE,Nakano JH

subject

Has Abstract

pub_date

1977-01-01 00:00:00

pages

613-23

issue

5

eissn

0042-9686

issn

1564-0604

journal_volume

55

pub_type

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