Rapid recruitment of hydrogen-producing biofilms for hydrogen production in a moving bed biofilm reactor by a sequential immobilization and deoxygenization approach.

Abstract:

:To reduce start-up time and enhance hydrogen production efficiency, a sequential immobilization and deoxygenization (SIDO) strategy for hydrogen production was investigated in continuous-flow moving bed biofilm reactors (MBBRs). The pre-immobilization process accelerated the initial enrichment of hydrogen-producing bacteria (HPB) and promoted the biofilm formation, which contribute to higher hydrogen production efficiency in SIDO-MBBRs compared to a non-immobilized reactor. A similar deoxygenization effect was achieved by inoculation with Pseudomonas aeruginosa compared with N2 sparging, and the P. aeruginosa pre-immobilized MBBR (Pse-MBBR) showed a higher H2 yield in the initial stage of operation. Microbial community analysis found a higher abundance of putative HPB in the range of 82.82-96.56%, with the predominant populations in the SIDO-MBBR assigned to genera Clostridium and Enterobacter. The results suggest that the SIDO-MBBR is an effective approach for rapid recruitment of HPB and start-up of fermentative hydrogen production.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Li Z,Wang J,Feng K,Li Y,Ding J,Liu B,Ren N,Xing D

doi

10.1016/j.biortech.2020.123979

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

123979

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(20)31251-7

journal_volume

317

pub_type

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