A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical methods.

Abstract:

:The production of a highly alkaline and thermostable pectinase of Bacillus pumilus was optimized in submerged fermentation using Plackett-Burman design and response surface methodology. Three fermentation variables (C:N ratio, K(2)HPO(4), and pH), which were identified to significantly affect pectinase production by Plackett-Burman design were further optimized using response surface methodology of central composite design (CCD). An over all 34- and 41-fold increase in enzyme production was achieved in shake flasks and lab fermenter by the optimization of variables using statistical approaches, respectively. The enzyme was optimally active at pH 10.5 and 50 degrees C, and selectively degraded only the noncellulosic gummy material of ramie (Boehmeria nivea) fibres causing 10.96% fibre weight loss, and therefore, the enzyme could find application in fibre processing industry. The use of the enzyme in fibre processing reduces the use of alkali, and the associated alkalinization of water bodies.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Sharma DC,Satyanarayana T

doi

10.1016/j.biortech.2005.04.012

subject

Has Abstract

pub_date

2006-03-01 00:00:00

pages

727-33

issue

5

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(05)00215-4

journal_volume

97

pub_type

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