Abstract：
PURPOSE:Prostate stem cell antigen (PSCA), a cell surface glycoprotein expressed in normal human prostate and bladder, is over-expressed in the majority of localized prostate cancer and most bone metastases. We have previously shown that the hu1G8 minibody, a humanized anti-PSCA antibody fragment (single-chain Fv-C(H)3 dimer, 80 kDa), can localize specifically and image PSCA-expressing xenografts at 21 h post-injection. However, the humanization and antibody fragment reformatting decreased its apparent affinity. Here, we sought to evaluate PET imaging contrast with affinity matured minibodies. METHODS:Yeast scFv display, involving four rounds of selection, was used to generate the three affinity matured antibody fragments (A2, A11, and C5) that were reformatted into minibodies. These three affinity matured anti-PSCA minibodies were characterized in vitro, and following radiolabeling with (124)I were evaluated in vivo for microPET imaging of PSCA-expressing tumors. RESULTS:The A2, A11, and C5 minibody variants all demonstrated improved affinity compared to the parental (P) minibody and were ranked as follows: A2 > A11 > C5 > P. The (124)I-labeled A11 minibody demonstrated higher immunoreactivity than the parental minibody and also achieved the best microPET imaging contrast in two xenograft models, LAPC-9 (prostate cancer) and Capan-1 (pancreatic cancer), when evaluated in vivo. CONCLUSION:Of the affinity variant minibodies tested, the A11 minibody that ranked second in affinity was selected as the best immunoPET tracer to image PSCA-expressing xenografts. This candidate is currently under development for evaluation in a pilot clinical imaging study.
journal_name
Eur J Nucl Med Mol Imagingauthors
Lepin EJ,Leyton JV,Zhou Y,Olafsen T,Salazar FB,McCabe KE,Hahm S,Marks JD,Reiter RE,Wu AMdoi
10.1007/s00259-010-1433-1subject
Has Abstractpub_date
2010-08-01 00:00:00pages
1529-38issue
8eissn
1619-7070issn
1619-7089journal_volume
37pub_type
杂志文章abstract：PURPOSE:An image-based scatter correction (IBSC) method was developed to convert scatter-uncorrected into scatter-corrected SPECT images. The purpose of this study was to validate this method by means of phantom simulations and human studies with 99mTc-labeled tracers, based on comparison with the conventional triple e...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-005-1791-2
更新日期：2005-10-01 00:00:00
abstract：PURPOSE:To evaluate the sensitivity and specificity of PET/CT findings in PMR and generate a diagnostic algorithm utilizing a minimum number of musculoskeletal sites. METHODS:Steroid-naïve patients with newly diagnosed PMR (2012 EULAR/ACR classification criteria) were prospectively recruited to undergo whole-body 18F-...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04731-z
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate metabolic and textural parameters from pretreatment [(18)F]FDG PET/CT scans for the prediction of neoadjuvant radiation chemotherapy response and 3-year disease-free survival (DFS) in patients with locally advanced rectal cancer (LARC). METHODS:We performed a retrospect...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-015-3180-9
更新日期：2016-03-01 00:00:00
abstract：:Vagus nerve stimulation (VNS) has gained recognition as a treatment for refractory epilepsies where surgical treatment is not possible. While it appears that this treatment is effective in some patients, the mechanism of action is not clearly understood. The purpose of this study was to clarify findings of other posit...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-1026-8
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:Nitroglycerin (NTG) is a vasodilating drug, which increases tumor blood flow and consequently decreases hypoxia. Therefore, changes in [18F] fluorodeoxyglucose positron emission tomography ([18F]FDG PET) uptake pattern may occur. In this analysis, we investigated the feasibility of [18F]FDG PET for response ass...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00259-016-3498-y
更新日期：2017-01-01 00:00:00
abstract：:This review describes the properties of available and emerging radiation detector and read-out technologies and discusses how they may affect PET scanner performance. After a general introduction, there is a section in which the physical properties of several different detector scintillators are compared. This is foll...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-003-1266-2
更新日期：2003-11-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate therapeutic response to neoadjuvant chemotherapy (NAC) and predict breast cancer recurrence using Positron Emission Tomography Response Criteria in Solid Tumors (PERCIST). MATERIALS AND METHODS:Fifty-nine breast cancer patients underwent fluorodeoxyglucose-positron emi...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,多中心研究
doi：10.1007/s00259-018-4008-1
更新日期：2018-09-01 00:00:00
abstract：:Breast cancer is associated with increased glucose consumption and can therefore be visualised with the glucose analogue [(18)F]2-deoxy-2-fluoro-D-glucose (FDG) and positron emission tomography (PET). FDG uptake in the primary tumour can vary substantially, and specific tumour characteristics have been demonstrated to...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-004-1529-6
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:The 18-kDa mitochondrial translocator protein (TSPO) was reported to be upregulated in gliomas. 18F-GE-180 is a novel 3rd generation TSPO receptor ligand with improved target-to-background contrast compared to previous tracers. In this pilot study, we compared PET imaging with 18F-GE-180 and MRI of patients w...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3799-9
更新日期：2017-12-01 00:00:00
abstract：INTRODUCTION:The precise clinical utility of lung 201Tl uptake in exercise SPECT myocardial perfusion imaging remains open to research. This study validates an optimal index for lung 201Tl uptake measurement and assesses its value in the prediction of higher-risk coronary artery disease (CAD). METHOD:Three hundred and...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-006-0255-7
更新日期：2007-04-01 00:00:00
abstract：PURPOSE:Overactivity of the multidrug efflux transporter P-glycoprotein (P-gp) at the blood-brain barrier (BBB) is believed to play an important role in resistance to central nervous system drug treatment. (R)-[(11)C]verapamil (VPM) PET can be used to measure the function of P-gp at the BBB, but low brain uptake of VPM...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1332-5
更新日期：2010-05-01 00:00:00
abstract：:The trade-off between resolution and count sensitivity dominates the performance of standard gamma cameras and dictates the need for relatively high doses of radioactivity of the used radiopharmaceuticals in order to limit image acquisition duration. The introduction of cadmium-zinc-telluride (CZT)-based cameras may o...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-016-3467-5
更新日期：2016-12-01 00:00:00
abstract：PURPOSE:Pretargeted radioimmunotherapy (PRIT) with bispecific antibodies (bsMAb) and a radiolabeled peptide reduces the radiation dose to normal tissues. Here we report the accuracy of an (111)In-labeled pretherapy test dose for personalized dosing of (177)Lu-labeled IMP288 following pretargeting with the anti-CEA × an...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-014-2742-6
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Rhenium-188-HEDP is a beta-emitting radiopharmaceutical used for palliation of metastatic bone pain. We investigated whether the addition of rhenium-188-HEDP to docetaxel/prednisone improved efficacy of chemotherapy in patients with CRPC. METHODS:Patients with progressive CRPC and osteoblastic bone metastas...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00259-017-3673-9
更新日期：2017-08-01 00:00:00
abstract：PURPOSE:Extracapsular spread (ECS) to the cervical lymph nodes is a major adverse prognostic factor in oral cavity squamous cell carcinoma (OSCC). We prospectively examined the value of FDG PET immediately before postoperative radiotherapy/concurrent chemoradiotherapy (pre-RT/CCRT PET) to detect residual/relapsing dise...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-012-2103-2
更新日期：2012-06-01 00:00:00
abstract：PURPOSE:The molecular features isocitrate dehydrogenase (IDH) mutation and 1p/19q co-deletion have gained major importance for both glioma typing and prognosis and have, therefore, been integrated in the World Health Organization (WHO) classification in 2016. The aim of this study was to characterize static and dynamic...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3846-6
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:Prostate-specific membrane antigen (PSMA) positron emission tomography (PET)/computed tomography (CT) is used for (re)staging prostate cancer (PCa) and as a biomarker for evaluating response to therapy, but lacks established response criteria. A panel of PCa experts in nuclear medicine, radiology, and/or urolog...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04934-4
更新日期：2021-02-01 00:00:00
abstract：PURPOSE:The purpose of this study was to monitor the metabolic effects of temozolomide (TMZ) chemotherapy in malignant gliomas by means of repeated positron emission tomography (PET) with [(11)C]methionine (MET). METHODS:Fifteen patients with histologically proven malignant glioma were treated by TMZ chemotherapy. MET...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-005-0002-5
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:The histamine H3 receptors are presynaptic neuroreceptors that inhibit the release of histamine and other neurotransmitters. The receptors are considered a drug target for sleep disorders and neuropsychiatric disorders with cognitive decline. We developed a novel PET ligand for the H3 receptors, [(11)C]TASP0410...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-016-3332-6
更新日期：2016-08-01 00:00:00
abstract：:This study was performed to assess the potential benefit of positron emission tomography (PET) using fluorine-18 fluorodeoxyglucose (FDG) in patients with cervical metastasis of squamous cell carcinoma from an unknown primary tumour. Eighteen patients with cervical metastasis of squamous cell carcinoma from an unknown...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-1078-9
更新日期：2003-03-01 00:00:00
abstract：PURPOSE:Cancer is still a clinical challenge, with many efforts invested in order to achieve timely detection. Unexplained elevated blood carcinoembryonic antigen levels are occasionally observed in an asymptomatic population and considered as a risk factor of cancers. The purpose of this study was to determine the val...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-015-3233-0
更新日期：2016-04-01 00:00:00
abstract：PURPOSE:The aim of this feasibility study was to use slice selective learning using a Generative Adversarial Network for external validation. We aimed to build a model less sensitive to PET imaging acquisition environment, since differences in environments negatively influence network performance. To investigate the sl...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04676-y
更新日期：2020-08-01 00:00:00
abstract：PURPOSE:Functional activation protocols are widely applied for the study of brain-cognition relations. Only few take advantage of the intrinsic characteristics of SPECT, particularly those allowing cognitive assessment outside of the camera, in settings close to the standard clinical or laboratory ones. The purpose of ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0635-7
更新日期：2008-04-01 00:00:00
abstract：:Multiple myeloma (MM) is characterized by a monoclonal plasma cell population in the bone marrow. Lytic lesions occur in up to 90 % of patients. For many years, whole-body X-ray (WBX) was the method of choice for detecting skeleton abnormalities. However, the value of WBX in relapsing disease is limited because lesion...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-016-3576-1
更新日期：2017-02-01 00:00:00
abstract：PURPOSE:To evaluate the role of F-18-Fluorothymidine (FLT) PET/CT in lymphoma patients with suspected recurrent or residual disease. METHODS:Adult lymphoma patients presenting with positive or equivocal F-18-FDG PET/CT at end-treatment or follow-up were prospectively addressed to an additional F-18-FLT-PET/CT. SUV max...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04323-6
更新日期：2019-07-01 00:00:00
abstract：PURPOSE:High-energy gamma probes have recently become commercially available, developed for (18)F-FDG probe-guided surgery. The radiation received by the staff in the operating room might limit the use of it, but has never been determined. We therefore wanted to measure the absorbed staff doses at operations where pati...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0532-0
更新日期：2008-03-01 00:00:00
abstract：:Oesophageal scintigraphy has been used for more than 25 years and allows quantifiable, safe, rapid, non-invasive and well-tolerated assessment of oesophageal transit time and function. As technology improves, more complex image analysis is becoming possible. Many studies have addressed its usefulness in assessing oeso...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-002-0826-1
更新日期：2002-09-01 00:00:00
abstract：PURPOSE:Alzheimer's disease (AD) is a primary degenerative disease that progressively affects all brain functions, with devastating consequences for the patient, the patient's family and society. Rest regional cerebral blood flow (rCBF) could have a strategic role in differentiating between AD patients and normal contr...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-005-1874-0
更新日期：2006-01-01 00:00:00
abstract：PURPOSE:The aim of this study was to evaluate the applicability of [(99m)Tc]Demotensin 5 and 6 [Formula: see text] in the targeted diagnostic imaging of neurotensin subtype 1 receptor (NTS1-R)-expressing tumours. METHODS:Labelling of Demotensin 5 and 6 with (99m)Tc was conducted by brief incubation with (99m)TcO(4) (-...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0489-z
更新日期：2007-11-01 00:00:00
abstract：:Dynamic PET (dPET) studies have been used until now primarily within research purposes. Although it is generally accepted that the information provided by dPET is superior to that of conventional static PET acquisitions acquired usually 60 min post injection of the radiotracer, the duration of dynamic protocols, the l...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-020-04843-6
更新日期：2021-01-01 00:00:00
