Abstract：
PURPOSE:The aim of this study was to evaluate the applicability of [(99m)Tc]Demotensin 5 and 6 [Formula: see text] in the targeted diagnostic imaging of neurotensin subtype 1 receptor (NTS1-R)-expressing tumours. METHODS:Labelling of Demotensin 5 and 6 with (99m)Tc was conducted by brief incubation with (99m)TcO(4) (-), SnCl(2) and citrate anions in alkaline medium at ambient temperature. Affinities of conjugates for the NTS1-R were determined by competition binding experiments in WiDr cell membranes using [(125)I-Tyr(3)]NT as the radioligand. Saturation binding assays were conducted for [(99m)Tc/(99g)Tc]Demotensin 6 in WiDr cell membranes. Internalisation of [(99m)Tc]Demotensin 5 and 6 was studied at 37 degrees C in WiDr cells. Biodistribution of [(99m)Tc]Demotensin 5 and 6 was performed in female Swiss nu/nu mice bearing human WiDr xenografts. RESULTS:Unlabelled conjugates showed a high affinity for the human NTS1-R (Demotensin 5 IC(50)=0.03+/-0.01 nM; Demotensin 6 IC(50)=0.08+/-0.02 nM), while high affinity was also exhibited by (radio)metallated [(99m)Tc/(99g)Tc]Demotensin 6 (K (d)=0.13+/-0.01 nM). [(99m)Tc]Demotensin 5 and 6 internalised rapidly and specifically in WiDr cells. After injection in WiDr tumour-bearing mice, radiopeptides, and especially the doubly stabilised [(99m)Tc]Demotensin 6, showed NTS1-R-mediated uptake in the intestines and in the implanted tumour (4.30+/-0.45%ID/g at 1 h post injection) and rapid renal excretion from non-target tissues into the urine. CONCLUSION:[(99m)Tc]Demotensin 6 shows a favourable preclinical profile and further testing in patients is warranted to monitor its eventual applicability as a radiotracer in the diagnostic imaging of NTS1-R-positive tumours.
journal_name
Eur J Nucl Med Mol Imagingauthors
Maina T,Nikolopoulou A,Stathopoulou E,Galanis AS,Cordopatis P,Nock BAdoi
10.1007/s00259-007-0489-zsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
1804-14issue
11eissn
1619-7070issn
1619-7089journal_volume
34pub_type
杂志文章abstract：PURPOSE:A pilot study was performed in patients with recurrent back pain after spinal fusion surgery to evaluate the ability of (18)F-NaF PET/CT imaging to correctly identify those requiring surgical intervention and to locate a site amenable to surgical intervention. METHODS:In this prospective study 22 patients with...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-012-2196-7
更新日期：2012-11-01 00:00:00
abstract：:Medical imaging has made a major contribution to cerebral dysfunction due to inherited diseases, as well as injuries sustained with modern living, such as car accidents, falling down, and work-related injuries. These injuries, up until the introduction of sensitive techniques such as positron emission tomography (PET)...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,收录出版
doi：10.1007/s00259-013-2401-3
更新日期：2013-09-01 00:00:00
abstract：INTRODUCTION:The precise clinical utility of lung 201Tl uptake in exercise SPECT myocardial perfusion imaging remains open to research. This study validates an optimal index for lung 201Tl uptake measurement and assesses its value in the prediction of higher-risk coronary artery disease (CAD). METHOD:Three hundred and...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-006-0255-7
更新日期：2007-04-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-002-0835-0
更新日期：2002-08-01 00:00:00
abstract：PURPOSE:The Alzheimer's disease (AD) pathology is characterized by fibrillar amyloid deposits and neurofibrillary tangles, as well as the activation of astrocytosis, microglia activation, atrophy, dysfunctional synapse, and cognitive impairments. The aim of this study was to test the hypothesis that astrocytosis is cor...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2014-11-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-001-0710-4
更新日期：2002-03-01 00:00:00
abstract：PURPOSE:To assess the localizing value of 18F-FDG PET in patients operated on for drug-resistant epilepsy due to focal cortical dysplasia type 2 (FCD2). METHODS:We analysed 18F-FDG PET scans from 103 consecutive patients (52 males, 7-65 years old) with histologically proven FCD2. PET and MRI data were first reviewed b...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
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更新日期：2010-01-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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abstract：PURPOSE:The study aims to investigate the role of 18F-alfatide positron emission tomography/computed tomography (PET/CT) in predicting the short-term outcome of concurrent chemoradiotherapy (CCRT) in patients with advanced non-small cell lung cancer (NSCLC). METHODS:Eighteen patients with advanced NSCLC had undergone ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3505-3
更新日期：2016-12-01 00:00:00
abstract：:Positron emission tomography (PET) with fluorine-18 fluorodeoxyglucose (FDG) is a very useful technique for the imaging of lymphomas in the adult population. It provides unique information about the behaviour of malignant cells and contributes to more accurate staging of the illness and better assessment of response t...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-002-0861-y
更新日期：2002-09-01 00:00:00
abstract：:This study investigated the value of fluorine-18 2'-deoxy-2-fluoro- D-glucose (FDG) imaging with a double-headed gamma camera operated in coincidence (hybrid PET) detection mode in patients with suspected spondylitis. Comparison was made with conventional nuclear medicine imaging modalities and magnetic resonance imag...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-001-0719-8
更新日期：2002-04-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-006-0110-x
更新日期：2006-10-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3443-0
更新日期：2016-12-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3432-3
更新日期：2016-12-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-003-1444-2
更新日期：2004-06-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-018-4210-1
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0659-z
更新日期：2008-04-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04574-3
更新日期：2020-02-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-005-1797-9
更新日期：2005-08-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-019-04348-x
更新日期：2019-08-01 00:00:00
abstract：PURPOSE:Radioligand binding studies show that beta(1)-adrenoceptor (beta(1)-AR) density may be reduced in heart disease without down regulation of beta(2)-ARs. Radioligands are available for measuring total beta-AR density non-invasively with clinical positron emission tomography (PET) but none are selective for beta(1...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0553-8
更新日期：2008-01-01 00:00:00
abstract：PURPOSE:Targeted therapy with α-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the α-emitter (213)Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined (213)Bi-diethylenetriaminepentaa...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-012-2203-z
更新日期：2012-12-01 00:00:00
abstract：:As measurement of glomerular filtration rate (GFR) is now generally the responsibility of departments of nuclear medicine, it is important for nuclear medicine physicians and scientists to understand the pharmacokinetics of the indicators and radiotracers that are used, generally known as filtration markers. The singl...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-003-1341-8
更新日期：2004-01-01 00:00:00
abstract：:Functional imaging of small animals, such as mice and rats, using ultra-high resolution positron emission tomography (PET) and single-photon emission tomography (SPET), is becoming a valuable tool for studying animal models of human disease. While several studies have shown the utility of PET imaging in small animals,...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-002-0776-7
更新日期：2002-05-01 00:00:00
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journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-011-1976-9
更新日期：2012-02-01 00:00:00
abstract：:Radioiodine therapy is now the most common definite treatment for persistent hyperthyroidism. The outcome of radioiodine therapy depends mainly on the absorbed energy dose in the diseased thyroid tissue. The administered activity and the resulting target dose in the thyroid depend on both the biokinetics of radioiodin...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-002-0868-4
更新日期：2002-08-01 00:00:00
abstract：PURPOSE:Evaluation of the long-term prognostic value of myocardial perfusion imaging (MPI) in octogenarians. METHODS:Six hundred and twenty-nine octogenarians [51% previous myocardial infarction (MI) or revascularization] who underwent single-isotope MPI (78% 201Tl, 22% 99mTc-tetrofosmin) with exercise (38% Bruce, 2% ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3739-8
更新日期：2017-08-01 00:00:00
