Abstract：
:Synthetic macromolecules such as copolymers of N-(2-hydroxypropyl)methacrylamide (pHPMA) are potential carriers for the delivery of drugs owing to their ability to passively accumulate in solid tumours [enhanced permeation and retention (EPR) effect]. To gain further knowledge about the biodistribution and the cellular localisation, poly(HPMA) was prepared for labelling by introducing biotin molecules. Biotinylated pHPMA (5 mol%) was intravenously injected into tumour-bearing rats and the accumulation of biotin-pHPMA was visualised using a streptavidin-alkaline phosphatase technique at day 7 post injection. In spite of the high solubility of pHPMA copolymers and the lack of attachment to cell structures, the biotinylated polymer could be easily detected in tissues fixed in 10% paraformaldehyde-phosphate buffer at 4 degrees C for 48 h. While biotin-pHPMA could be detected intracytoplasmically in liver and spleen, a predominantly interstitial localisation was observed within the anaplastic prostate carcinoma (Dunning R3327-AT1). How biotin as a label influences the biodistribution of poly(HPMA) was assessed by scintigraphy, autoradiography and histology comparing homopolymer poly(HPMA) with biotin-pHPMA. The organ distribution patterns of the two polymers correlated well, except with respect to kidney. It is assumed that the accumulation of biotin-pHPMA in the distal tubuli is due to a biotin transporter in the brush border membrane. The technique presented is useful for a more comprehensive understanding of the biodistribution of soluble macromolecules.
journal_name
Eur J Nucl Med Mol Imagingauthors
Kissel M,Peschke P,Subr V,Ulbrich K,Strunz AM,Kühnlein R,Debus J,Friedrich Edoi
10.1007/s00259-002-0835-0subject
Has Abstractpub_date
2002-08-01 00:00:00pages
1055-62issue
8eissn
1619-7070issn
1619-7089journal_volume
29pub_type
杂志文章abstract：PURPOSE:The serotonin-1A (5-HT(1A)) receptor is implicated in the pathophysiology of major neuropsychiatric disorders. Thus, the functional imaging of 5-HT(1A) receptors by positron emission tomography (PET) may contribute to the understanding of its role in those pathologies and their therapeutics. These receptors exi...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1274-y
更新日期：2010-03-01 00:00:00
abstract：PURPOSE:Despite modern CT systems and expert evaluators, the diagnostic performance of coronary CT angiography is limited by overestimation of vessel stenosis which reduces the positive predictive value (PPV) of the test. The aim of this study was to evaluate the performance of combined cardiac PET/64-detector CT angio...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1213-y
更新日期：2009-12-01 00:00:00
abstract：:In Alzheimer's disease (AD), the deposition of β-amyloid (Aβ) is hypothesized to result in a series of secondary neurodegenerative processes, leading ultimately to synaptic dysfunction and neuronal loss. Since the advent of the first Aβ-specific positron emission tomography (PET) ligand, (11)C-Pittsburgh compound B ([...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-015-3208-1
更新日期：2016-02-01 00:00:00
abstract：:Nuclear medicine techniques are becoming more important in imaging oncological and infectious diseases. For metabolic imaging of these diseases, antibody and peptide imaging are currently used. In recent years peptide imaging has become important, therefore the rationale for the use of peptide imaging is described in ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-011-2038-z
更新日期：2012-02-01 00:00:00
abstract：PURPOSE:Improved bifunctional chelates (BFCs) are needed to facilitate efficient (64)Cu radiolabeling of monoclonal antibodies (mAbs) under mild conditions and to yield stable, target-specific agents. The utility of two novel BFCs, 1-Oxa-4,7,10-triazacyclododecane-5-S-(4-isothiocyanatobenzyl)-4,7,10-triacetic acid (p-S...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-010-1506-1
更新日期：2010-11-01 00:00:00
abstract：PURPOSE:(11)C-PK11195 is a radiopharmaceutical for in vivo assessment of peripheral benzodiazepine receptor (PBR) activity using PET. We sought to clarify the metabolic fate of (11)C-PK11195 in a test-retest setting using radio-HPLC in comparison with radio-TLC, and the whole-body distribution in humans. MATERIALS AND...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-008-1000-1
更新日期：2009-04-01 00:00:00
abstract：PURPOSE:Myocardial perfusion with PET/CT has advantages over conventional SPECT. We describe our initial European experience using (82)Rubidium-PET/CT, as part of a clinical myocardial perfusion service. METHODS:We studied the first 100 patients (64 male; 36 female, mean age = 60: SD +/-12.5y, mean body mass index = 3...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-007-0537-8
更新日期：2007-12-01 00:00:00
abstract：PURPOSE:Point-spread function (PSF) or PSF + time-of-flight (TOF) reconstruction may improve lesion detection in oncologic PET, but can alter quantitation resulting in variable standardized uptake values (SUVs) between different PET systems. This study aims to validate a proprietary software tool (EQ.PET) to harmonize ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,多中心研究
doi：10.1007/s00259-015-3128-0
更新日期：2015-12-01 00:00:00
abstract：PURPOSE:The usefulness of (18)F-FDG PET/CT for bone metastasis evaluation has already been established. The amino acid PET tracer [(18)F]-3-fluoro-alpha-methyl tyrosine ((18)F-FAMT) has been reported to be highly specific for malignancy. We evaluated the additional value of (18)F-FAMT PET/CT to complement (18)F-FDG PET...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-013-2487-7
更新日期：2013-10-01 00:00:00
abstract：PURPOSE:Targeted therapy with α-particle emitting radionuclides is a promising new option in cancer therapy. Stable conjugates of the vascular tumour-homing peptide F3 with the α-emitter (213)Bi specifically target tumour cells. The aim of our study was to determine efficacy of combined (213)Bi-diethylenetriaminepentaa...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-012-2203-z
更新日期：2012-12-01 00:00:00
abstract：PURPOSE:The purpose of this study was to monitor the metabolic effects of temozolomide (TMZ) chemotherapy in malignant gliomas by means of repeated positron emission tomography (PET) with [(11)C]methionine (MET). METHODS:Fifteen patients with histologically proven malignant glioma were treated by TMZ chemotherapy. MET...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-005-0002-5
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:This study investigated the diagnostic performance and prognostic value of fluorodeoxyglucose (FDG) positron emission tomography (PET)/CT in suspected malignant pleural mesothelioma (MPM) recurrence, in the context of patterns and intensity of FDG uptake, histologic type, and treatment algorithm. METHODS:Fifty...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-010-1704-x
更新日期：2011-05-01 00:00:00
abstract：PURPOSE:To study the feasibility of the in vivo [18F]-DPA-714 TSPO positron emission tomography (PET) to detect glial activation in a rat model of progressive parkinsonism induced by viral-mediated overexpression of A53T mutated human α-synuclein (hα-syn) in the substantia nigra pars compacta (SNpc). METHODS:We conduc...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04772-4
更新日期：2020-10-01 00:00:00
abstract：PURPOSE:Primary tumor (PT) and metastatic lymph node (MLN) status have a great influence on diagnosis and treatment of lung cancer. Our main purpose was to investigate the imaging characteristics of PT or MLN by applying the 18F-FDG PET dynamic modeling approach for non-small cell lung cancer (NSCLC). METHODS:Dynamic ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-020-04682-5
更新日期：2020-05-01 00:00:00
abstract：PURPOSE:To investigate the value of 68Ga-HBED-CC PSMA (68Ga-PSMA) PET/CT for response assessment in metastatic castration-sensitive and castration-resistant prostate cancer (mCSPC and mCRPC) during docetaxel chemotherapy. METHODS:68Ga-PSMA PET/CT was performed in seven mCSPC patients before and after six cycles of upf...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3887-x
更新日期：2018-04-01 00:00:00
abstract：:Vagus nerve stimulation (VNS) has gained recognition as a treatment for refractory epilepsies where surgical treatment is not possible. While it appears that this treatment is effective in some patients, the mechanism of action is not clearly understood. The purpose of this study was to clarify findings of other posit...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-1026-8
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:We compared the prognostic value of 99mTc-tetrofosmin single-photon emission computed tomography (SPECT) after nitrate administration and positron emission tomography (PET) with 18F-fluorodeoxyglucose (FDG) in patients with ischaemic left ventricular (LV) dysfunction. METHODS:Eighty-nine patients with previous...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-006-0304-2
更新日期：2007-04-01 00:00:00
abstract：:Jérôme Barriere was inadvertently missing in the original version of this article. He has participated to the study design, protocol writing and inclusion of a significant number of patients. ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 已发布勘误
doi：10.1007/s00259-019-04321-8
更新日期：2019-07-01 00:00:00
abstract：PURPOSE:The purpose of this study was to evaluate the usefulness of L-4-borono-2-18F-fluoro-phenylalanine (18F-FBPA) as a tumor-specific probe, in comparison to 18F-FDG and 11C-methionine (Met), focusing on its transport selectivity by L-type amino acid transporter 1 (LAT1), which is highly upregulated in cancers. MET...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3487-1
更新日期：2017-02-01 00:00:00
abstract：PURPOSE:Cervical dystonia (CD) is associated with tremor/jerks (50%) and psychiatric complaints (17-70%). The dopaminergic system has been implicated in the pathophysiology of CD in animal and imaging studies. Dopamine may be related to the motor as well as non-motor symptoms of CD. CD is associated with reduced striat...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3664-x
更新日期：2017-08-01 00:00:00
abstract：PURPOSE:Overactivity of the multidrug efflux transporter P-glycoprotein (P-gp) at the blood-brain barrier (BBB) is believed to play an important role in resistance to central nervous system drug treatment. (R)-[(11)C]verapamil (VPM) PET can be used to measure the function of P-gp at the BBB, but low brain uptake of VPM...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-009-1332-5
更新日期：2010-05-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate metabolic and textural parameters from pretreatment [(18)F]FDG PET/CT scans for the prediction of neoadjuvant radiation chemotherapy response and 3-year disease-free survival (DFS) in patients with locally advanced rectal cancer (LARC). METHODS:We performed a retrospect...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-015-3180-9
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:The purpose of this study was to design a fully automatic computer-assisted diagnostic system for early- and late-onset mild Alzheimer's disease (AD). METHODS:Glucose metabolic images were obtained from mild AD patients and normal controls using positron emission tomography (PET) and( 18)F-fluorodeoxyglucose (...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-005-0015-0
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:To compare the performance characteristics of positron emission mammography (PEM) with those of whole-body PET (WBPET) and PET/CT in women with newly diagnosed breast cancer. METHODS:A total of 178 women consented to PEM for presurgical planning in an IRB-approved protocol and also underwent either WBPET (n = ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-013-2553-1
更新日期：2014-02-01 00:00:00
abstract：:The goal of this study was to perform a systematic, semi-quantitative analysis of limbic perfusion in patients with Alzheimer's disease (AD) using coregistered single-photon emission tomography (SPET) images aligned to magnetic resonance (MR) images. Limbic perfusion in 40 patients with mild to moderate AD was compare...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-002-0816-3
更新日期：2002-07-01 00:00:00
abstract：PURPOSE:We tested the hypothesis that lateralized hemispheric glucose metabolism may have diagnostic implications in Alzheimer's disease (AD) and mild cognitive impairment (MCI). METHODS:We performed FDG-PET/CT in 23 patients (mean age 63.7 years, range 50-78, 17 females) diagnosed with AD (n = 15) or MCI (n = 8) duri...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-018-4248-0
更新日期：2019-05-01 00:00:00
abstract：PURPOSE:Focal congenital hyperinsulinism (CHI) is curable by surgery, which is why identification of the focal lesion is crucial. We aimed to determine the use of 18F-fluoro-dihydroxyphenylalanine (18F-DOPA) PET/CT vs. 68Ga-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic-acid-1-Nal3-octreotide (68Ga-DOTANOC) PET/CT...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-017-3867-1
更新日期：2018-02-01 00:00:00
abstract：INTRODUCTION:Ischemia in hypertrophic cardiomyopathy (HCM) is caused by coronary microvascular dysfunction (CMD), which is detected by measuring myocardial blood flow (MBF) with PET. Whether CMD may be associated with ischemic left ventricular (LV) dysfunction is unclear. We therefore assessed LV ejection fraction (EF)...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章
doi：10.1007/s00259-016-3603-2
更新日期：2017-05-01 00:00:00
abstract：:In locally advanced head and neck squamous cell carcinoma (HNSCC), the role of imaging becomes more and more critical in the management process. In this framework, molecular imaging techniques such as PET allow noninvasive assessment of a range of tumour biomarkers such as metabolism, hypoxia and proliferation, which ...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 杂志文章,评审
doi：10.1007/s00259-014-2972-7
更新日期：2015-04-01 00:00:00
abstract：INTRODUCTION:A targeted theragnostic approach based on increased expression of prostate-specific membrane antigen (PSMA) on PC cells is an attractive treatment option for patients with metastatic castration-resistant prostate cancer (mCRPC). METHODS:Ten consecutive mCRPC patients were selected for 177Lu-PSMA617 therap...
journal_title：European journal of nuclear medicine and molecular imaging
pub_type： 临床试验,杂志文章
doi：10.1007/s00259-016-3609-9
更新日期：2017-05-01 00:00:00
