Chymosin and other milk coagulants: sources and biotechnological interventions.

Abstract:

:Calf rennet, which consists of over 90% chymosin, is commonly used in cheese industries for the curdling of milk. Various animal, plant and microbial sources have been exploited as possible alternatives to calf rennet. The coagulating properties of the enzymatic preparations (coagulants) from these sources differ in terms of their physicochemical factors. The cheese industry has always sought out novel and stable enzyme sources, and recombinant chymosin has been found to be an effective alternative since it possesses several advantages over plant and microbial milk-clotting enzymes. This paper reviews the use of various milk coagulants, especially animal coagulants, for cheese making. Advancements in genetic and protein engineering to produce recombinant chymosin are discussed in addition to evaluating its identity to the rennet available from natural sources.

journal_name

Crit Rev Biotechnol

authors

Kumar A,Grover S,Sharma J,Batish VK

doi

10.3109/07388551.2010.483459

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

243-58

issue

4

eissn

0738-8551

issn

1549-7801

journal_volume

30

pub_type

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