Abstract:
:We have developed and validated a high-performance liquid chromatography method coupled with a mass detector to quantify itraconazole, voriconazole, and posaconazole using quinoxaline as the internal standard. The method involves protein precipitation with acetonitrile. Mean accuracy (percent deviation from the true value) and precision (relative standard deviation percentage) were less than 15%. Mean recovery was more than 80% for all drugs quantified. The lower limit of quantification was 0.031 microg/ml for itraconazole and posaconazole and 0.039 microg/ml for voriconazole. The calibration range tested was from 0.031 to 8 microg/ml for itraconazole and posaconazole and from 0.039 to 10 microg/ml for voriconazole.
journal_name
Antimicrob Agents Chemotherjournal_title
Antimicrobial agents and chemotherapyauthors
Baietto L,D'Avolio A,Ventimiglia G,De Rosa FG,Siccardi M,Simiele M,Sciandra M,Di Perri Gdoi
10.1128/AAC.01807-09subject
Has Abstractpub_date
2010-08-01 00:00:00pages
3408-13issue
8eissn
0066-4804issn
1098-6596pii
AAC.01807-09journal_volume
54pub_type
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