Abstract:
INTRODUCTION:Renal graft dysfunction due to acute rejection, acute tubular necrosis, or anticalcineurin toxicity with development of interstitial fibrosis or tubular atrophy are the primary causes of graft failure. Determination of kidney function using the serum creatinine concentration demonstrates low sensitivity as a marker for the diagnosis, and kidney biopsy is an invasive procedure. The levels of urinary messenger RNA of genes that regulate epithelial-mesenchymal transition (EMT) can reflect early kidney damage. Thus, repeated transcriptome studies of these genes can provide information about the evolution of the graft, and possibly enable early diagnosis and treatment. OBJECTIVE:To analyze the temporal relationships between early graft evolution and gene expression of EMT biomarkers. METHODS:Of 70 kidney transplant procedures performed between January 1, 2007 and December 31, 2008, 42 were analyzed prospectively for 3 months. Analytical and clinical data were recorded, as well as histologic findings if available. Urine mRNA extraction was performed using a commercially available kit. RNA gene expression of EMT, angiotensinogen, epidermal growth factor receptor, E-cadherin, N-cadherin, transforming growth factor-β, and bone morphogenetic protein 7 was determined at real-time quantitative polymerase chain reaction. β2-Microglobulin was used as a reference gene. RESULTS:At 75 days posttransplantation, analysis revealed that angiotensinogen (mean [SD], 2.91 [0.70] vs 6.04 [1.24]; P=.04) and N-cadherin (1.01 [0.43] vs 4.31 [0.92]; P=.01) discriminate good evolution from acute rejection. Epidermal growth factor receptor (2.78 [0.66] vs 6.02 [1.09]; P=.33) and bone morphogenetic protein 7 (0.85 [0.33] vs 3.07 [1.37]; P=.04) discriminate good evolution vs delayed graft function. CONCLUSION:Differential gene expression at 75 days posttransplantation reflects differences related to initial histologic damage. This observation encourages design of a long-term longitudinal analysis with multiple markers to obtain early diagnosis and forecast the prognosis of graft dysfunction.
journal_name
Transplant Procjournal_title
Transplantation proceedingsauthors
Gomez-Alamillo C,Benito-Hernandez A,Ramos-Barron MA,Agüeros C,Rodrigo E,Ruiz JC,Sanchez M,San Cosme L,Arias Mdoi
10.1016/j.transproceed.2010.07.051subject
Has Abstractpub_date
2010-10-01 00:00:00pages
2886-8issue
8eissn
0041-1345issn
1873-2623pii
S0041-1345(10)01096-1journal_volume
42pub_type
杂志文章abstract:BACKGROUND:Egypt has the highest prevalence of the world hepatitis C virus (HCV) load. Hence, the problem of end-stage liver disease (ESLD) is considered a huge burden on the community. Living donor liver transplantation (LDLT) is the only source of donation in Egypt till now. Survival rates had shown significant impro...
journal_title:Transplantation proceedings
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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doi:10.1016/j.transproceed.2016.01.060
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journal_title:Transplantation proceedings
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doi:10.1016/j.transproceed.2012.01.135
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Transplantation proceedings
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章,评审
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更新日期:2018-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.transproceed.2003.08.010
更新日期:2003-11-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章
doi:10.1016/j.transproceed.2013.08.052
更新日期:2013-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.transproceed.2006.01.040
更新日期:2006-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1978-03-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章,评审
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更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章
doi:10.1016/j.transproceed.2008.07.141
更新日期:2008-12-01 00:00:00
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journal_title:Transplantation proceedings
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-04-01 00:00:00