HPV mRNA tests for the detection of cervical intraepithelial neoplasia: a systematic review.


OBJECTIVE:Perform a systematic review to determine the test performance of HPV mRNA testing compared to DNA testing using CIN2+ as the target condition. METHODS:We searched bibliographic databases (MEDLINE, EMBASE and Cochrane Library) from January 1996 through August 2010 using a predefined search strategy. The reference standard used to diagnose precancerous lesions was histologically confirmed cervical intraepithelial neoplasia 2+ (CIN2+). Two reviewers independently assessed study eligibility, extracted data, and assessed risk of bias. Sensitivity, specificity, positive and negative likelihood ratios and diagnostic odds ratios were calculated for each study. In addition, we fitted a series of summary receiver operating characteristics (SROC) curves. A subgroup analysis was performed according to specific inclusion covariates. RESULTS:Out of 3179 potentially relevant citations, 12 publications (11 studies) met our inclusion criteria. The included studies were of varying methodological quality, and were predominately performed in a secondary screening setting. Eight studies investigated the performance of the PreTect Proofer/NucliSENS EasyQ, two studies investigated the performance of the APTIMA assay and one study investigated both mRNA tests on the same patient samples. Due to few studies and considerable clinical heterogeneity, pooling of data was not possible. Instead, we compiled a 'best evidence synthesis' for E6/E7 mRNA HPV testing. Sensitivities ranged from 0.41 to 0.86 and from 0.90 to 0.95 for the PreTect Proofer/Easy Q and APTIMA assay, respectively. Specificities ranged from 0.63 to 0.97 and from 0.42 to 0.61 for the PreTect Proofer/Easy Q and APTIMA assay, respectively. The SROC curves for both mRNA tests were to the left of the diagonal and the APTIMA assay performed closest to the DNA tests. CONCLUSION:The review suggests that mRNA tests have diagnostic relevance, but additional studies and economic evaluations must be conducted in order to make a solid conclusion regarding the clinical applicability of HPV mRNA testing.


Gynecol Oncol


Gynecologic oncology


Burger EA,Kornør H,Klemp M,Lauvrak V,Kristiansen IS




Has Abstract


2011-03-01 00:00:00














  • Extraperitoneal laparoscopic lymph node staging: the University of Southern California experience.

    abstract:OBJECTIVES:To describe our experience with extraperitoneal lymph node staging in gynecologic oncology. MATERIALS AND METHODS:The extraperitoneal approach was performed to assess the lymph node histology in patients with gynecologic malignancies. The nodes are approached from a lateral approach after dissecting open th...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Burnett AF,O'Meara AT,Bahador A,Roman LD,Morrow CP

    更新日期:2004-10-01 00:00:00

  • Risk-specific follow-up for endometrial carcinoma patients.

    abstract:OBJECTIVE:To propose a risk-specific follow-up protocol for endometrial carcinoma patients. METHODS:A retrospective cohort of endometrial carcinoma patients was used to identify risk factors for recurrence. Based on a profile of risk factors, women were classified at either low or high risk for recurrence (median foll...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Shumsky AG,Brasher PM,Stuart GC,Nation JG

    更新日期:1997-06-01 00:00:00

  • Class III NSRH: oncological outcome in 170 cervical cancer patients.

    abstract:OBJECTIVE:To analyze local recurrence rate (LRR), morbidities and oncologic outcome of class III nerve-sparing radical hysterectomy. PATIENTS AND METHODS:170 consecutive class III NSRH cases were performed. Nineteen patients were addressed directly to surgery whilst neoadjuvant chemotherapy was administered in 151 pat...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Ditto A,Martinelli F,Hanozet F,Reato C,Solima E,Zanaboni F,Grijuela B,Carcangiu M,Haeusler E,Raspagliesi F

    更新日期:2010-11-01 00:00:00

  • Metastatic dermatofibrosarcoma protuberans of the vulva.

    abstract::Dermatofibrosarcoma protuberans (DFSP) of the vulva is a rare, low- to intermediate-grade sarcoma of dermal origin. This case report represents the 13th reported patient with vulvar DFSP and the first reported patient with metastatic vulvar DFSP. Although metastasis is rare, the possibility remains that the frequent r...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Soergel TM,Doering DL,O'connor D

    更新日期:1998-11-01 00:00:00

  • Treatment of malignant ascites with allogeneic and autologous lymphokine-activated killer cells.

    abstract::Two cases of peritonitis carcinomatosa with gynecological cancer, which did not respond to conventional treatment, were treated by intraperitoneal adoptive immunotherapy with allogeneic or autologous lymphokine-activated killer (LAK) cells plus interleukin-2. In case 1, reduction of acute ascites and disappearance of ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Kamada M,Sakamoto Y,Furumoto H,Mori K,Daitoh T,Irahara M,Aono T,Nii A,Yanagawa H,Sone S

    更新日期:1989-07-01 00:00:00

  • The incidence and risk factors associated with postoperative delirium in geriatric patients undergoing surgery for suspected gynecologic malignancies.

    abstract:BACKGROUND:The incidence of postoperative delirium (PD) in the elderly ranges between 3-60% but has never been examined in gynecologic oncology. Our goal was to identify pre, intra, and postoperative risk factors associated with the development of PD. METHODS:English speaking women of 60 years and above undergoing maj...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: McAlpine JN,Hodgson EJ,Abramowitz S,Richman SM,Su Y,Kelly MG,Luther M,Baker L,Zelterman D,Rutherford TJ,Schwartz PE

    更新日期:2008-05-01 00:00:00

  • Conservative management of early stage cervical cancer: is there a role for less radical surgery?

    abstract:OBJECTIVES:The utility of parametrial resection in women with early stage cervical cancer is controversial. In patients with favorable pathologic characteristics such as tumor size <2cm and the absence of lymphovascular space invasion, the rate of parametrial involvement is very low. More conservative surgical approach...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Schmeler KM,Frumovitz M,Ramirez PT

    更新日期:2011-03-01 00:00:00

  • Comparison of bevacizumab alone or with chemotherapy in recurrent ovarian cancer patients.

    abstract:BACKGROUND:To compare the efficacy of chemotherapy (C) combined with bevacizumab (Bev) versus Bev alone in recurrent, heavily pretreated epithelial ovarian cancer (EOC). METHODS:A multicenter analysis of patients treated from 2004 to 2011 was performed. Demographic, treatment, response, and adverse event information w...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,多中心研究


    authors: Fuh KC,Secord AA,Bevis KS,Huh W,ElNaggar A,Blansit K,Previs R,Tillmanns T,Kapp DS,Chan JK

    更新日期:2015-12-01 00:00:00

  • Phase II study of axalimogene filolisbac (ADXS-HPV) for platinum-refractory cervical carcinoma: An NRG oncology/gynecologic oncology group study.

    abstract:OBJECTIVE:Women with persistent, recurrent, and/or metastatic cervical cancer have a poor prognosis. Even with the availability of cisplatin plus paclitaxel and bevacizumab, median overall survival (OS) is only 17.0 months, with median post-progression survival of approximately seven months. We studied the therapeutic ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Huh WK,Brady WE,Fracasso PM,Dizon DS,Powell MA,Monk BJ,Leath CA 3rd,Landrum LM,Tanner EJ,Crane EK,Ueda S,McHale MT,Aghajanian C

    更新日期:2020-09-01 00:00:00

  • Characterization of CSOC 882, a novel immortalized ovarian cancer cell line expressing EGFR, HER2, and activated AKT.

    abstract:OBJECTIVE:Only a small number of comprehensively characterized immortalized ovarian cancer cell lines are available for laboratory studies on ovarian cancer. We describe a new ovarian cancer cell line that arose from primary culture of a stage IC, grade III ovarian carcinoma, designated CSOC 882. METHODS:We characteri...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Scoles DR,Pavelka J,Cass I,Tran H,Baldwin RL,Armstrong K,Karlan BY

    更新日期:2007-01-01 00:00:00

  • Endometrial stromal sarcoma: objective response to letrozole.

    abstract:BACKGROUND:Low-grade endometrial stromal sarcoma is generally an indolent tumor rich in estrogen and progesterone receptors. Objective responses to hormonal therapy, most commonly with megestrol acetate, have been reported. CASE:The patient is a 51-year-old woman who presented with low-grade endometrial stromal sarcom...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Maluf FC,Sabbatini P,Schwartz L,Xia J,Aghajanian C

    更新日期:2001-08-01 00:00:00

  • Single-dose actinomycin D in the treatment of postmolar trophoblastic disease.

    abstract::From 1973 to 1982 single-dose (0.04 mg/kg or 1.25 mg/m2), biweekly actinomycin D was used to treat 28 women with postmolar, good-prognosis trophoblastic disease. Among the 17 patients fully evaluable for response to treatment 12 were nonmetastatic (NMTD) and 5 had lung metastases (MTD). Nine NMTD patients and 5 MTD pa...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Schlaerth JB,Morrow CP,Nalick RH,Gaddis O Jr

    更新日期:1984-09-01 00:00:00

  • A new score based on biomarker values to predict the prognosis of locally advanced cervical cancer.

    abstract:OBJECTIVE:To define a prognostic score based on pretreatment values of leucocyte, platelet and hemoglobin in locally advanced cervical cancer (LACC). MATERIAL AND METHODS:We conducted a prospective study of 238 patients for LACC with negative PET imaging in the para-aortic (PA) area and who were undergoing laparoscopi...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Maulard A,Chargari C,Faron M,Alwohaibi A,Leary A,Pautier P,Genestie C,Morice P,Gouy S

    更新日期:2020-11-01 00:00:00

  • Optimal surgical debulking in uterine papillary serous carcinoma affects survival.

    abstract:OBJECTIVE:UPSC is similar to papillary serous ovarian carcinoma in its histology and pattern of spread. The survival advantage with optimal debulking for ovarian cancer has been demonstrated. We examined our experience with UPSC. METHODS:Seventy-eight UPSC patients were seen between 1995 and 2008 at Rush University Me...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Patsavas K,Woessner J,Gielda B,Rotmensch J,Yordan E,Bitterman P,Guirguis A

    更新日期:2011-06-01 00:00:00

  • Stage II endometrioid adenocarcinoma of the endometrium: clinical implications of cervical stromal invasion.

    abstract:OBJECTIVES:Endometrioid adenocarcinoma of the endometrium (EEC) is the most common histologic type of endometrial cancer, with stage being the most critical prognostic factor. Cervical involvement (CI), divided into IIA (epithelial involvement) and IIB (stromal invasion), is overall associated with decreased survival (...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Orezzoli JP,Sioletic S,Olawaiye A,Oliva E,del Carmen MG

    更新日期:2009-06-01 00:00:00

  • Phase II clinical trial of capecitabine in ovarian carcinoma recurrent 6-12 months after completion of primary chemotherapy, with exploratory TS, DPD, and TP correlates: a Gynecologic Oncology Group study.

    abstract:PURPOSE:A phase II trial was conducted to evaluate the anti-tumor activity and adverse effects of capecitabine in women with measurable platinum-sensitive ovarian cancer or platinum-sensitive primary peritoneal cancer and to explore the ability of thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), and th...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Garcia AA,Blessing JA,Lenz HJ,Darcy KM,Mannel RS,Miller DS,Husseinzadeh N,Gynecologic Oncology Group.

    更新日期:2005-03-01 00:00:00

  • Does the interval from primary surgery to chemotherapy influence progression-free survival in ovarian cancer?

    abstract:OBJECTIVES:The objective of this study was to determine whether the length of the interval from primary surgery to commencement of chemotherapy has any direct effect on progression-free survival in ovarian cancer. METHODS:The progression-free survival of 472 patients enrolled in four trials who had all received platin...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Flynn PM,Paul J,Cruickshank DJ,Scottish Gynaecological Cancer Trials Group.

    更新日期:2002-09-01 00:00:00

  • Tamoxifen and the risk of ovarian cancer in BRCA1 mutation carriers.

    abstract:OBJECTIVE:BRCA1 mutation carriers have a high rate of both breast and ovarian cancer. Tamoxifen is a selective estrogen receptor modulator (SERM), which is used for the treatment of primary breast cancer and for the prevention of contralateral breast cancer. Our objective is to assess if tamoxifen treatment is associat...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Vicus D,Rosen B,Lubinski J,Domchek S,Kauff ND,Lynch HT,Isaacs C,Tung N,Sun P,Narod SA,Hereditary Ovarian Cancer Clinical Study Group.

    更新日期:2009-10-01 00:00:00

  • Quality of laparoscopic radical hysterectomy in developing countries: a comparison of surgical and oncologic outcomes between a comprehensive cancer center in the United States and a cancer center in Colombia.

    abstract:OBJECTIVE:To help determine whether global collaborations for prospective gynecologic surgery trials should include hospitals in developing countries, we compared surgical and oncologic outcomes of patients undergoing laparoscopic radical hysterectomy at a large comprehensive cancer center in the United States and a ca...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Pareja R,Nick AM,Schmeler KM,Frumovitz M,Soliman PT,Buitrago CA,Borrero M,Angel G,Reis RD,Ramirez PT

    更新日期:2012-05-01 00:00:00

  • Phase II evaluation of oxaliplatin in previously treated squamous cell carcinoma of the cervix: a gynecologic oncology group study.

    abstract:OBJECTIVE:A phase II study was conducted to determine the efficacy of oxaliplatin therapy in patients with previously treated squamous cell carcinoma of the cervix. METHODS:Eligible patients were to have measurable disease and not more than one prior chemotherapy regimen that could include carboplatin or cisplatin but...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Fracasso PM,Blessing JA,Wolf J,Rocereto TF,Berek JS,Waggoner S

    更新日期:2003-07-01 00:00:00

  • Expression and clinical role of long non-coding RNA in high-grade serous carcinoma.

    abstract:OBJECTIVE:To profile long non-coding RNA (lncRNA) expression at the various anatomic sites of high-grades serous carcinoma (HGSC) and in effusion-derived exosomes. METHODS:LncRNA profiling was performed on 60 HGSC specimens, including 10 ovarian tumors, 10 solid metastases and 10 malignant effusions, as well as exosom...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Filippov-Levy N,Cohen-Schussheim H,Tropé CG,Hetland Falkenthal TE,Smith Y,Davidson B,Reich R

    更新日期:2018-03-01 00:00:00

  • The effect of beta-adrenergic stimulation on the bladder and urethra following radical hysterectomy.

    abstract::The effect of two beta-adrenergic stimulating agents on bladder and urethral function was assessed in 16 patients 3 weeks after radical hysterectomy for carcinoma of the cervix. Urodynamic evaluation was performed before and after inhalation of metaproterenol by metered dose inhaler in 10 patients and injection of 0.2...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章


    authors: Kerr-Wilson RH,Orr JW Jr,Shingleton HM,Hatch KD,Trammel MW

    更新日期:1986-03-01 00:00:00

  • Primary biliary carcinoma with metastasis to the ovary.

    abstract::The ovary is a relatively frequent site of metastasis from malignant neoplasia arising elsewhere in the body, the majority of these originating from the GI tract. The best known tumor of this type is signet-ring cell adenocarcinoma (Krukenberg tumor) of gastric origin. The gall bladder and bile ducts are rare sources ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章,评审


    authors: Lashgari M,Behmaram B,Hoffman JS,Garcia J

    更新日期:1992-11-01 00:00:00

  • Intraepithelial neoplasia in the vulvar region.

    abstract::From 1978 to 1982, 49 patients with intraepithelial neoplasia in the vulvar region were included in a prospective investigation. The main purpose was to evaluate the effect of treatment by local excision with a free margin of 2 mm and to estimate the importance of colposcopy. In 28% of the patients, the disease recurr...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Andreasson B,Bock JE

    更新日期:1985-07-01 00:00:00

  • Prediction of short-term surgical complications in women undergoing pelvic exenteration for gynecological malignancies.

    abstract:OBJECTIVE:Pelvic exenteration (PE) is an extensive surgery associated with high rates of postoperative morbidity and mortality. The absence of well-defined preoperative selection criteria to identify patients eligible for PE prompted the assessment of pre-operative predictors of 30-day major surgical complications. ME...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Tortorella L,Casarin J,Mara KC,Weaver AL,Multinu F,Glaser GE,Cliby WA,Scambia G,Mariani A,Kumar A

    更新日期:2019-01-01 00:00:00

  • Prospective assessment of survival, morbidity, and cost associated with lymphadenectomy in low-risk endometrial cancer.

    abstract:OBJECTIVE:Since 1999, patients with low risk endometrial cancer (EC) as defined by the Mayo criteria have preferably not undergone lymphadenectomy (LND) at our institution. Here we prospectively assess survival, sites of recurrence, morbidity, and cost in this low risk cohort. METHODS:Cause-specific survival (CSS) was...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Dowdy SC,Borah BJ,Bakkum-Gamez JN,Weaver AL,McGree ME,Haas LR,Keeney GL,Mariani A,Podratz KC

    更新日期:2012-10-01 00:00:00

  • Possible prognostic significance of human papillomavirus type in cervical cancer.

    abstract::While several different human papillomaviruses (HPV) have been associated with cancer of the cervix, it is yet to be determined if specific HPV types have clinical or prognostic significance. To address this question, 30 cases of invasive carcinoma (squamous carcinoma, adenosquamous carcinoma, and adenocarcinoma) with...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Barnes W,Delgado G,Kurman RJ,Petrilli ES,Smith DM,Ahmed S,Lorincz AT,Temple GF,Jenson AB,Lancaster WD

    更新日期:1988-03-01 00:00:00

  • Chlamydial cervicitis and cervical intraepithelial neoplasia: an immunohistochemical analysis.

    abstract::Studies using serological and culture techniques indicate that chlamydial infection is frequently associated with cervical intraepithelial neoplasia (CIN). This relationship was investigated by examining a series of biopsies containing normal epithelium, non-neoplastic condylomatous epithelium, and neoplastic epitheli...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Mitao M,Reumann W,Winkler B,Richart RM,Fujiwara A,Crum CP

    更新日期:1984-09-01 00:00:00

  • Risk-based stratification of carcinomas concurrently involving the endometrium and ovary.

    abstract:OBJECTIVE:Determining whether carcinomas concurrently involving endometrium and ovary are independent primary tumors (IPTs) or endometrial carcinomas with ovarian metastases (at least stage IIIA endometrial cancers, IIIA-EC) using clinicopathologic criteria is often challenging. Recent genomic studies showed that most ...

    journal_title:Gynecologic oncology

    pub_type: 杂志文章


    authors: Turashvili G,Gómez-Hidalgo NR,Flynn J,Gonen M,Leitao MM Jr,Soslow RA,Murali R

    更新日期:2019-01-01 00:00:00

  • A multicenter phase II study of gemcitabine, paclitaxel, and cisplatin in chemonaïve advanced ovarian cancer.

    abstract:OBJECTIVES:The objectives of this multicenter phase II study were to evaluate the effects of gemcitabine-paclitaxel-cisplatin combination chemotherapy on response rate, survival, and toxicity in patients with advanced epithelial ovarian cancer (AEOC). METHODS:Chemonaive AEOC patients with bidimensionally measurable di...

    journal_title:Gynecologic oncology

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Gupta SK,John S,Naik R,Arora R,Selvamani B,Fuloria J,Ganesh N,Awasthy BS

    更新日期:2005-07-01 00:00:00