当前位置： SCI文献检索 > TRANSPLANTATION PROCEEDINGS期刊下所有文献 > Transfection of hairpin small interfering RNA expression vector targeting rat nuclear factor (NF) (κB) inhibits rat cell proliferation induced by NF-κB signal pathway activation.

Transfection of hairpin small interfering RNA expression vector targeting rat nuclear factor (NF) (κB) inhibits rat cell proliferation induced by NF-κB signal pathway activation.

Abstract：

OBJECTIVE:The aim of this work was to construct one small interfering RNA (siRNA) eukaryotic expression vector targeting rat nuclear factor (NF)κB p65 and identify its inhibition effect on cell proliferation according to its down-regulation of NF-κB pathway. METHODS:The p65siRNA expression vector "pGenesil-1.2-p65siRNA" and negative control plasmid "HK" were transfected into the cultured rat cells. After transfection, cells were divided into 4 treatment groups: 1) control cells cultured in complete. Dulbecco modified Eagle medium; 2) lipopolysaccharide (LPS) (1 μg/mL); (3) LPS (1 μg/mL) + HK-transfected; 4) LPS (1 μg/mL) + p65siRNA (pGenesil-1.2-p65siRNA). Thereafter, the protein levels of NF-κB p65 in the cells were detected by Western blotting at 72 hours after LPS stimulation. Furthermore, to observe cell proliferation, the proliferative rate of the cell growth was evaluated by the methylthiazolyl tetrazolium assay (at 24, 48, and 72 hours). The cell cycle distribution at 72 hours was detected by flow cytometry. RESULTS:p65siRNA effectively down-regulated the protein level of p65 (P < .05). Meanwhile, the proliferation of cells transfected with p65siRNA expression vector was significantly inhibited (P < .05), the ratio of cells at G(0)/G(1) stage markedly increased, and the proportion of cells at S stage was significantly decreased among transfected compared with control cells (P < .05). CONCLUSIONS:p65siRNA effectively suppressed NF-κB, expression, inhibiting rat cell proliferation induced by NF-κB signal pathway activation.

journal_name

Transplant Proc

journal_title

Transplantation proceedings

authors

He XP,Li XX,Wang ZH,Bi YW,Liu FY,Liu XY

doi

10.1016/j.transproceed.2010.09.168

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

4633-7

issue

10

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(10)01604-0

journal_volume

42

pub_type

杂志文章

相关文献

TRANSPLANTATION PROCEEDINGS文献大全
  • Hemophagocytic Syndrome as Uncommon Cause of Severe Pancytopenia After Liver Transplantation.

    abstract：BACKGROUND:A rare but life-threatening cause of pancytopenia after liver transplantation is hemophagocytic syndrome. We present a 48-year-old woman who underwent liver transplantation and developed a hemophagocytic syndrome secondary to Epstein-Barr virus with a fatal course, despite initial treatment with immunosuppre...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2020.02.060

    authors： Valenzuela EF,García AC,García-Pajares F,Tejada JT,Muñoz RN,Martín CM,Delgado LS,Martín CA,Álvarez CA,Fernández-Fontecha E,Sánchez-Antolín G

    更新日期：2020-06-01 00:00:00

  • Why we consistently use the left donor kidney in living related transplantation: initial experience of right laparoscopic donor nephrectomy and comparison with left nephrectomy.

    abstract：OBJECTIVES:Laparoscopic donor nephrectomy has become the method of choice for removal of living donor kidneys. However, the majority of laparoscopic donor nephrectomy cases have been limited to the left side owing to technical difficulties and renal vessel length. This study described the technique and compared donor o...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2006.06.023

    authors： Liu KL,Chiang YJ,Wu CT,Lai WJ,Wang HH,Chu SH

    更新日期：2006-09-01 00:00:00

  • Antibody-mediated response in rat liver chronic rejection.

    abstract：PURPOSE:The aim was to study the effect of an antibody-mediated response on rat chronic liver rejection. METHODS:Liver transplantation was performed in rats assigned to one of the 3 treatment groups: group A, Brown Norway (BN) to BN + CsA; group B, dark Agouti (DA) to BN; and group C, DA to BN + CsA. In addition to su...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2011.03.043

    authors： Wan R,Ying W,Zeng L,Deng X,Chen H,Gao L

    更新日期：2011-06-01 00:00:00

  • Optimizing outcomes by preconditioning the donor.

    abstract：:The criteria that define a so-called "marginal donor" kidney have been standardized since 2002. However, every transplant center must establish its own guidelines on organ acceptability. An expanded criteria donor (ECD) kidney is age at least 60 years, or 50 to 59 years with at least two of three specified comorbiditi...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2010.12.020

    authors： Lledó-Garcia E,Subirá-Ríos D,Tejedor-Jorge A,del Cañizo-López JF,Hernández-Fernández C

    更新日期：2011-01-01 00:00:00

  • Changes in notification and demographics of deceased donors during the past decade in the state of Michigan, USA.

    abstract：:From 1993 to 2003 there have been significant changes in the number and demographics of deceased donors referred to the organ procurement organization (OPO) in the state of Michigan (USA). It was the aim of this study to document the magnitude of these changes and attempt to explain them. There has been a 26-fold incr...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2004.12.023

    authors： Rosenberg JC,Beyersdorf T,Pietroski R

    更新日期：2005-03-01 00:00:00

  • Serum level of soluble fibrinogen-like protein 2 in renal allograft recipients with acute rejection: a preliminary study.

    abstract：BACKGROUND:Soluble fibrinogen-like protein 2 (sfgl2), which is mainly secreted by T cells, is a novel effector of regulatory T cells with immunosuppressive functions. The aim of this study was to investigate serum levels of sfgl2 among renal allograft recipients. METHODS:From November 2010 to August 2011 we retrospect...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2012.05.082

    authors： Zhao Z,Yang C,Tang Q,Zhao T,Jia Y,Ma Z,Rong R,Xu M,Zhu T

    更新日期：2012-12-01 00:00:00

  • Tacrolimus and Mycophenolic Acid Blood Concentration and Cellular Rejection After Heart Transplantation in First Endomyocardial Biopsy.

    abstract：BACKGROUND:Tacrolimus and mycophenolic acid (MPA) are the most important immunosuppressive drugs in modern heart transplantation. The pharmacokinetics of tacrolimus are best described by a 2-compartment model. MPA has very variable pharmacokinetics. The aim of this research was to compare kinetics of the immunosuppress...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2020.03.006

    authors： Nadziakiewicz P,Grochla M,Krauchuk A,Szyguła-Jurkiewicz B,Lorek J,Barańska A,Zembala MO,Przybyłowski P

    更新日期：2020-09-01 00:00:00

  • Expanded criteria donor kidneys for younger recipients: acceptable outcomes.

    abstract：INTRODUCTION:European senior programme (ESP) is well known for acceptable outcomes using expanded criteria donor (ECD) kidneys from donors older than 65 years for recipients older than 65 years. The incidence of end-stage renal disease (ESRD) is 229/million in India with a mean age of 45 years. We performed a retrospec...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2010.09.040

    authors： Goplani KR,Kute VB,Vanikar AV,Shah PR,Gumber MR,Patel HV,Modi PR,Trivedi HL

    更新日期：2010-12-01 00:00:00

  • Cholangiocarcinoma, 10-Year Survival After Liver Transplantation: A Case Report.

    abstract：:Cholangiocarcinoma is the second most common neoplasm in the liver, with a very poor, short-term prognosis. Today, surgery associated with or without an adjuvant is the only curative treatment. Liver transplantation (LT) is the best treatment for hepatocellular carcinoma tumor. In recent years, treatment of hilar chol...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2019.12.030

    authors： Herrero Torres MA,Dominguez Bastante M,Molina Raya A,Palomo Lopez I,Santoyo Villalba J,Villegas Herrera MT,Becerra Massare A,Brea Gomez E,Zambudio Carroll N,Villar Del Moral JM

    更新日期：2020-03-01 00:00:00

  • Protective effects of sirolimus by attenuating connective tissue growth factor expression in human chronic allograft nephropathy.

    abstract：BACKGROUND:Chronic allograft nephropathy (CAN) remains a great challenge for the transplant clinician. The introduction of sirolimus (RAPA) with cyclosporine (CsA) reduction maybe shed new light to improve graft survival. The aim of this study was to investigate the overall effects of sirolimus conversion on biopsy-pro...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.transproceed.2007.03.072

    authors： Liu M,Zhang W,Gu M,Yin C,Zhang WY,Lv Q,Xu D

    更新日期：2007-06-01 00:00:00

  • A comparative study of the traditional method, and a point-score system for allocation of deceased-donor kidneys: a national multicenter study in Mexico.

    abstract：BACKGROUND:The National Transplant Center in Mexico has ruled that deceased-donor kidney allocation is a function of each hospital's Internal Transplant Committee. The aim of this study was to compare and analyze results for of the traditional method and a point-score system in the allocation of deceased patient's kidn...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.transproceed.2011.09.091

    authors： Cantú-Quintanilla G,Alberú J,Reyes-Acevedo R,Medeiros M,Villa MS,Arreola JM,Gracida C,Reyes-López A

    更新日期：2011-11-01 00:00:00

  • Solid Organ Transplantation in HIV+ Recipients: Italian Experience.

    abstract：INTRODUCTION:According to current estimates, there are about 540,000 patients who are infected with HIV in Western Europe, of which about 3100 are potential candidates for organ transplantation. In Italy, there are currently 85 HIV patients on the transplant list. METHODS:Organ transplantation activity in HIV recipien...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2015.12.049

    authors： Morabito V,Grossi P,Lombardini L,Ricci A,Trapani S,Peritore D,Gaeta A,Ballanti D,Del Sordo E,Rizzato L,Nanni Costa A

    更新日期：2016-03-01 00:00:00

  • Short- and Long-Term Outcomes of Live Donor Renal Allografts From Older and Younger Donors.

    abstract：BACKGROUND:The rising demand for living renal donors has led to the recruitment of older donors. Findings vary, but these grafts appear to survive as long as grafts from standard criteria deceased and expanded criteria deceased donors. We investigated the effects of donor age ≥65 years and the presence or absence of do...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2018.08.046

    authors： Matsuyama T,Ushigome H,Osaka M,Masuda K,Harada S,Nakamura T,Nobori S,Iida T,Yoshimura N

    更新日期：2018-12-01 00:00:00

  • Atenolol attenuates autonomic dysfunction of rat jejunum submitted to cold ischemic preservation.

    abstract：:In vitro studies have demonstrated that cold ischemic preservation (CIP) employed in small bowel transplantation produces loss of intestinal motility due to severe lesions of autonomic enteric nerves and that this autonomic dysfunction is attenuated by antioxidant agents. In this work, we investigated whether preserva...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2006.05.024

    authors： Taha MO,Fraga MM,Guimarães FA,Jurkiewicz A,Caricati-Neto A

    更新日期：2006-07-01 00:00:00

  • Interleukin-4 induces lipogenesis in porcine endothelial cells, which in turn is critical for induction of protection against complement-mediated injury.

    abstract：:Interleukin (IL)-4 has been shown to induce protection in porcine vascular endothelial cells (ECs) from killing by human complement. This protection is dependent on the PI3K/Akt signaling pathway. In this study, we investigated mechanisms downstream of Akt and found that activation of the lipid biosynthesis pathway is...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.02.013

    authors： Black SM,Schott ME,Benson BA,Rutherford MS,Young BK,Dalmasso AP

    更新日期：2008-03-01 00:00:00

  • São Paulo lung transplantation waiting list: patient characteristics and predictors of death.

    abstract：INTRODUCTION:Nowadays, lung transplantation (LTx) allocation in Brazil is based mainly on waiting time. There is a need to evaluate the equity of the current lung allocation system. OBJECTIVES:We sought to (1) determine the characteristics of registered patients on the waiting list and (2) identify predictors of death...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2009.01.048

    authors： Costa da Silva F Jr,Afonso JE Jr,Pêgo-Fernandes PM,Caramori ML,Jatene FB

    更新日期：2009-04-01 00:00:00

  • Intestinal water and electrolyte absorption and secretion.

    abstract：:Diarrhea is a frequent complication of small bowel transplantation in patients and experimental models. Although there are many potential causes, the underlying basis for most are not fully understood. Improved therapeutic approaches will come about through better understanding of mechanisms of bowel adaptation and re...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,评审

    doi：

    authors： Chang EB

    更新日期：1996-10-01 00:00:00

  • Transplantation in Congenital Heart Disease: A Challenge.

    abstract：BACKGROUND:Heart failure is the leading cause of death in grown-up congenital heart disease patients (GUCH). Although heart transplantation (OHT) remains the gold standard in end-stage heart failure, the ratio of GUCH patients undergoing this procedure remains low. OBJECTIVE:Describe the cohort of GUCH patients underg...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2019.12.027

    authors： Palomo-López N,Escalona-Rodríguez S,Martín-Villén L,Herruzo-Avilés Á,Hinojosa-Pérez R,Escoresca-Ortega A,Porras-López M,Corcia-Palomo Y,Adsuar-Gómez A

    更新日期：2020-03-01 00:00:00

  • Novel Application of Internal-External Drainage Catheter as Biliary Stent for Percutaneous Transhepatic Treatment of Biliary Strictures in Living Donor Liver Transplantation Recipient Patients.

    abstract：OBJECTIVES:Although endoscopic management is considered as the first-line treatment for biliary strictures, it may be challenging in living donor liver transplant recipients due to the complex nature of duct-to-duct reconstruction. In this study we present the use of a pigtail drainage catheter as a biliary stent to tr...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2019.01.153

    authors： Server S,Sabet S,Sahin T,Guven K,Aydın U,Tokat Y,Inan N

    更新日期：2019-09-01 00:00:00

  • A necessary step toward kidney donor safety: the transition from locking polymer clips to transfixion techniques in laparoscopic donor nephrectomy.

    abstract：:Donor safety is of paramount importance in addressing end-stage renal failure through living kidney transplantation. The United States Food and Drug Administration (FDA) issued a Class II recall on the use of Hem-o-lok (Teleflex, Limerick, Pennsylvania, United States) polymer clips on the renal artery in laparoscopic ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2013.11.120

    authors： Goh YS,Cheong PS,Lata R,Goh A,Vathsala A,Li MK,Tiong HY

    更新日期：2014-01-01 00:00:00

  • Pretreatment with the tumor nerosis factor-alpha blocker etanercept attenuated ischemia-reperfusion renal injury.

    abstract：INTRODUCTION:Tumor necrosis factor (TNF)-alpha mediates inflammation and apoptosis in ischemia-reperfusion (IR) injury of the kidneys. Etanercept, a soluble TNF-alpha receptor, has shown anti-inflammatory and anti-apoptotic effects in several animal models of renal injury, including chronic insufficiency and unilateral...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2009.05.042

    authors： Choi DE,Jeong JY,Lim BJ,Na KR,Shin YT,Lee KW

    更新日期：2009-11-01 00:00:00

  • Laparoscopic treatment of biliary peritonitis after removal of T-tube in liver transplant patients.

    abstract：INTRODUCTION:T-tube removal in liver transplant patients can occasionally cause a massive biliary leak and may require surgical treatment for its resolution. We present our experience with a laparoscopic approach to biliary peritonitis in liver transplant patients after the removal of a T-tube. PATIENTS AND METHODS:Fr...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2012.05.018

    authors： Cascales Campos P,Ramírez Romero P,González R,Pons JA,Miras M,Sanchez Bueno F,Robles R,Parrilla P

    更新日期：2012-07-01 00:00:00

  • Laparoscopic hepatectomy for a modified right graft in adult-to-adult living donor liver transplantation.

    abstract：BACKGROUND:We performed a modified right hepatectomy completely by laparoscopic techniques preserving the middle hepatic vein (MHV) branches in adult-to-adult living donor liver transplantation (LDLT). PATIENTS AND METHODS:Two young women (24 and 25 years old) volunteered to be live donors for their parents who had he...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2008.07.134

    authors： Suh KS,Yi NJ,Kim J,Shin WY,Lee HW,Han HS,Lee KU

    更新日期：2008-12-01 00:00:00

  • Functional and histological comparison of rat liver preserved in University of Wisconsin solution compared with tissue preserved in a novel solution.

    abstract：:An isolated perfused rat liver model was used to investigate biochemical and histologic changes during 2 hours of reperfusion after 24 hours of cold storage to compare Leeds solution (LS) with University of Wisconsin solution (UW). Compared with livers stored in UW, those perfused with LS showed significantly higher b...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2010.06.029

    authors： Corps CL,Ahmed I,McKenzie S,Shires M,Potts DJ,Lodge JP

    更新日期：2010-11-01 00:00:00

  • Living donor transplant: wider selection criteria.

    abstract：:The availability of cadaveric donor organs is insufficient for actual needs. The organ demand increases by 20% per year. Living donor transplant (LDT) may be a valid therapeutical alternative provided one uses proper criteria. LDT provides many advantages, like improved patient and organ survival, short waiting time, ...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2004.02.003

    authors： Splendiani G,Cipriani S,Valeri M,Torlone N,Vega A,Tullio T,Condò S,Dominijanni S,Casciani CU

    更新日期：2004-04-01 00:00:00

  • Robot-Assisted Radical Prostatectomy in a Post-Kidney Transplant Patient: An Initial Case Report in China.

    abstract：OBJECTIVE:Our aim was to evaluate the general applicability, feasibility, and safety of robot-assisted radical prostatectomy (RARP) in Chinese renal allograft patients for prostate cancer removal. PATIENTS AND METHODS:A 62-year-old patient diagnosed as having biopsy-proven localized prostate cancer in March 2016 who h...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2018.09.015

    authors： Fang Y,Chen Z,Juan L,Feng Z,Cao J,Zhou B,Huang Y,Cen J,Lu J,Liang Y,Wei J,Luo J,Chen W

    更新日期：2018-12-01 00:00:00

  • Living donor kidney transplantation: chance for the recipient--financial risk for the donor?

    abstract：BACKGROUND:With living donation, in addition to the medical risk, the financial risk for the donor is essential, especially in case of complications that potentially can led to disability and loss of work. We report the experiences of those who have donated a kidney in our transplant center. METHODS:We contacted 80 do...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/s0041-1345(03)00675-4

    authors： Wolters HH,Heidenreich S,Senninger N

    更新日期：2003-09-01 00:00:00

  • Rituximab after autologous stem cell transplantation enhances survival of B-cell lymphoma patients: a meta-analysis and systematic review.

    abstract：BACKGROUND:Numerous studies have demonstrated the efficacy of rituximab before autologous stem cell transplantation (ASCT) for the treatment of B-cell non-Hodgkin lymphoma, but the few studies on rituximab treatment after ASCT have not established conclusively the clinical benefits of this particular treatment regimen....

    journal_title：Transplantation proceedings

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.transproceed.2014.11.040

    authors： He G,Wang C,Tan H,He S

    更新日期：2015-03-01 00:00:00

  • Reduced and standard target concentration tacrolimus with sirolimus in renal allograft recipients.

    abstract：:We report 6-month results of renal allograft recipients enrolled in seven Australian centers as part of a worldwide, multicenter, randomized, open-label, concentration-controlled trial comparing standard tacrolimus (sTAC) with reduced tacrolimus (rTAC) both with sirolimus (SRL) and steroids. Patients were randomized 1...

    journal_title：Transplantation proceedings

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/s0041-1345(03)00210-0

    authors： Russ GR,Campbell S,Chadban S,Eris J,O'Connell P,Pussell B,Walker R,Australian Rapamune-Tacrolimus Study Group.

    更新日期：2003-05-01 00:00:00

  • Massive degeneration and atrophy of the native heart after heterotopic transplantation: a case report.

    abstract：:Extreme myocardial degeneration leading to advanced stages of cardiomyopathy with extensive atrophy is rarely observed before patients die. However, heterotopic transplantation is a special situation wherein this phenomenon can be observed. The greater part of the failed heart shows recuperation after receiving circul...

    journal_title：Transplantation proceedings

    pub_type： 杂志文章

    doi：10.1016/j.transproceed.2009.02.012

    authors： Fiorelli AI,Coelho GH,Lima JL,Lourenço DD,Gutierres P,Bacal F,Bocchi E,Dias RR,Stolf NA

    更新日期：2009-04-01 00:00:00