Abstract：
PURPOSE:Growth factors are considered as key molecules that participating in fibrosis formation. This research aimed to clarify potential effects of p53 on regulation of transforming growth factor β (TGF-β) and fibrosis formation and investigate the associated mechanisms. METHODS:Vimentin was examined to identify human Tenon's fibroblasts (HTFs). p53-targeting small interfere RNA (siRNA) was synthesis and transfected into HTFs. Real-time PCR assay was utilized to evaluate p53 and microRNA-29b (miR-29b) expression. Immunocytochemical assay was used to observe TGF-β expression. The wound healing assay was conducted to evaluate migration of HTFs. Dual-luciferase assay was employed to identify interaction between p53 and miR-29b in HTFs. RESULTS:Vimentin was extensively distributed in HTFs cells. HTFs at density of 5 × 104 cells/ml and 6 days exhibited the best growth. The p53 level in TGF-β treatment group was significantly higher compared to that in blank group (p < 0.01). miR-29b level in siRNA targeting p53 group was significantly increased compared to that in blank group (p < 0.01). siRNA targeting p53 could significantly inhibit HTFs migration compared to that in single TGF-β treating HTFs group (p < 0.01). Relative luciferase activity was significantly increased in p53 overexpressed HTFs compared to that in cells transfected with empty pcDNA3.0 plasmid (p < 0.01). CONCLUSIONS:p53 inhibited expression of TGF-β, suppressed HTFs migration and inhibited HTFs growth, by reducing miR-29b expression and interacting with miR29b gene in HTFs.
journal_name
Int Ophthalmoljournal_title
International ophthalmologyauthors
Li N,Zhang Rdoi
10.1007/s10792-020-01320-9subject
Has Abstractpub_date
2020-06-01 00:00:00pages
1509-1516issue
6eissn
0165-5701issn
1573-2630pii
10.1007/s10792-020-01320-9journal_volume
40pub_type
杂志文章abstract：PURPOSE:Early predictors of diabetic complications may aid in the prevention and/or management of these complications. The aim of this cross-sectional study was to determine the predictive value of retinopathy for the presence of other diabetic complications. METHODS:The population studied comprised 648 patients with ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1023/a:1014409829614
更新日期：2001-01-01 00:00:00
abstract：:Lipoid proteinosis is a rare disorder involving multiple organ systems. The ocular manifestations are usually trivial, but greatly aid in the diagnosis. We describe a patient who presented to us with typical moniliform blepharosis. A brief description of relevance to the ophthalmologist and review of the literature fo...
journal_title：International ophthalmology
pub_type： 杂志文章,评审
doi：10.1007/s10792-011-9417-0
更新日期：2011-04-01 00:00:00
abstract：:The objective of this study is to evaluate the visual prognosis and postoperative course in advanced glaucoma patients who underwent trabeculectomy. The records of 30 patients with advanced visual field (VF) defects undergoing trabeculectomy were retrospectively reviewed. Severe VF defects were defined as those with a...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-011-9494-0
更新日期：2011-12-01 00:00:00
abstract：:This study aims to report a case of acute macular neuroretinopathy (AMN) misdiagnosed as optic neuritis. The methods include review of the clinical, photographic, angiographic, visual field, infrared imaging, and optical coherence tomography records of a patient with AMN. A 30-year-old female who was initially diagnos...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-014-0024-8
更新日期：2015-02-01 00:00:00
abstract：:This paper builds on results of a previous paper on the prevalence of trachomatous visual impairment as the foundation for assessing the global burden of trachomatous blindness and low vision: approximately 2.9 million cases of trachomatous blindness and 3.8 million low visioned corresponding to a global prevalence of...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/BF00130921
更新日期：1995-01-01 00:00:00
abstract：:To investigate subconjunctival bevacizumab as an adjuvant in first-time glaucoma filtration surgery, we conducted a non-comparative, interventional case series, enrolling consecutive patients with uncontrolled primary glaucomas. All patients underwent trabeculectomy with mitomycin C and received a 1.25 mg subconjuncti...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-012-9704-4
更新日期：2013-12-01 00:00:00
abstract：:To analyze the pattern of intermediate uveitis (IU) in a referral center in Tunisia, North Africa. A retrospective, descriptive study of 87 consecutive patients (145 eyes) examined at the Department of Ophthalmology of Monastir (Tunisia) from January 1996 to August 2008. All patients underwent an extensive ocular and ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-010-9352-5
更新日期：2010-10-01 00:00:00
abstract：PURPOSE:To determine whether there are angiographic differences among normal, preperimetric and advanced glaucoma eyes using indocyanine green angiography with SLO. This method was chosen because of its sensibility to detect peripapillary capillary vessels. METHODS:Scanning laser opthalmoscopy was preformed on normal ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1023/a:1014431404096
更新日期：2001-01-01 00:00:00
abstract：:This study reports the outcomes of therapeutic penetrating keratoplasties, defined as keratoplasties performed to eradicate active infectious corneal diseases or to repair a structural defect of the cornea. The records of 24 eyes of 24 patients (17 female and 7 male) treated with therapeutic penetrating keratoplasty b...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-014-9931-y
更新日期：2015-04-01 00:00:00
abstract：:To investigate the efficacy of single-dose intravitreal dexamethasone implantation in the treatment of persistent diabetic macular edema (DME) unresponsive to 3 consecutive ranibizumab injections over a period of 6 months. Forty-one patients with a previous history of treatment for DME including at least three consecu...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-015-0155-6
更新日期：2016-08-01 00:00:00
abstract：PURPOSE:Regional variations of the preoperative biometry can affect the refractive accuracy of cataract surgery. We aimed to compare the preoperative biometric data for cataract surgery between two domestic institutions. METHODS:We retrospectively reviewed the preoperative biometric data of 673 consecutive eyes underg...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-020-01476-4
更新日期：2020-11-01 00:00:00
abstract：PURPOSE:To compare the efficacy of intravitreal injection of ranibizumab, dexamethasone implant and aflibercept for the management of macular edema (ME) related to branch retinal vein occlusion (BRVO). METHODS:This retrospective and comparative study included 62 eyes of 62 patients with BRVO and ME. Patients received ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-017-0618-z
更新日期：2018-08-01 00:00:00
abstract：:The purpose of the study was to compare the accuracy of various biometric formulas for predicting postoperative refraction determined using applanation A-scan ultrasound. This retrospective comparative study included 485 eyes that underwent uneventful phacoemulsification with intraocular lens (IOL) implantation. Appla...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-016-0195-6
更新日期：2016-10-01 00:00:00
abstract：:Oximetry may be useful for understanding pathologies of the eye. We use a prototype scanning laser ophthalmoscope capable of simultaneous multiple wavelength imaging to record fundus images. The system is run under non-confocal conditions in using slit apertures with a width of up to 600 microm. The laser lines launch...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1023/a:1014406831412
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:To investigate the natural course, visual outcome, and risk factors for visual loss after nonarteritic anterior ischemic optic neuropathy (NAION) attack in Chinese patients from a tertiary medical center in Southern Taiwan. METHODS:This is a longitudinal observational study that included sixty NAION patients, ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-020-01448-8
更新日期：2020-10-01 00:00:00
abstract：PURPOSE:To assess safety and efficacy of deep topical anesthesia with ropivacaine-soaked sponge compared with topical anesthesia with oxybuprocaine in patients undergoing phacoemulsification. METHODS:This was a retrospective study where records of patients operated for cataract were evaluated. Patients using a visual ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-019-01132-6
更新日期：2019-12-01 00:00:00
abstract：PURPOSE:To investigate the characteristics of dynamic visual acuity (DVA) and contrast sensitivity (CS) in pseudophakic patients with posterior capsular opacification (PCO). METHODS:Fifty-four eyes (36 patients) with PCO planned for laser capsulotomy were recruited. They underwent examinations of static visual acuity ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-020-01536-9
更新日期：2020-12-01 00:00:00
abstract：:We report the functional outcome of a patient who had undergone Boston keratoprosthesis type I for extensive ocular surface damage caused by latex of Euphorbia. The milky sap or latex of the Euphorbia plant is highly toxic and an irritant to the skin and eye. Damage to the eye ranges from mild to severe corneal edema,...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-012-9515-7
更新日期：2012-02-01 00:00:00
abstract：:We report a case of transient ectropion associated with corneal ulcer. The corneal ulcer responded well to treatment with fortified gentamicin and cefazolin. As the corneal ulcer healed, the eyelid simultaneously returned to its original position. We suggest that in the presence of involutional eyelid changes, even a ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1023/a:1006004320762
更新日期：1997-01-01 00:00:00
abstract：:The purpose of this study is to evaluate whole lysyl oxidase like 1 (LOXL1) gene by sequence analysis in Turkish patients with exfoliation glaucoma (XFG). A total of 48 (35 male, 13 female) patients with XFG were enrolled. Besides routine ophthalmological examination, peripapillary retinal nerve fibre layer (RNFL) ana...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-016-0174-y
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:To evaluate whether retinal arteriolar widths change in normal patients and in different glaucoma types. We measured the arteriolar width at the merge in the optic nerve (MD), at the edge of the optic nerve (ED) and at the peripapillary area (1 disc diameter distance) (disc distance D). HRT software 1.11, Inter...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1023/a:1014417606396
更新日期：2001-01-01 00:00:00
abstract：:The aim of this study is to determine the incidence of cystoid macular edema (CME) following repositioning and McCannel iris-suturing of dislocated intraocular lenses. This study is conducted in an urban private practice. A retrospective chart review was performed on consecutive patients who presented with posteriorly...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-014-9964-2
更新日期：2014-10-01 00:00:00
abstract：:Ocular wall dimensions (axial eye length, anterior chamber depth, lens thickness, vitreous length, thickness and volume of the ocular wall) were determined by echobiometry on 10 eyes of 10 patients with glaucoma one day before and four days after trabeculectomy. The results showed significant differences: decreased ax...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/BF00917990
更新日期：1992-09-01 00:00:00
abstract：PURPOSE:To evaluate the long-term safety and efficacy of infliximab therapy for refractory uveitis associated with Behçet's disease (BD). METHODS:We prospectively enrolled six patients who failed to respond to conventional immunosuppressive treatment. Infliximab infusions (5 mg/kg) were administered at weeks 0, 2, 6, ...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-006-9006-9
更新日期：2005-06-01 00:00:00
abstract：INTRODUCTION:Vogt-Koyanagi-Harada syndrome (VKHS) is an inflammatory systemic autoimmune disease principally affecting pigmented tissues in the ocular, auditory, integumentary and central nervous systems. Patients are generally women in the fourth decade of life. The prognosis is correlated mainly with the time between...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-017-0504-8
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:We carried out a study by in-vivo confocal microscopy to investigate the appearance of iridocorneal endothelial (ICE) syndrome, and discuss its diagnostic potential. METHODS:Twelve patients, each with unilateral ICE syndrome, had both their eyes examined by in-vivo confocal microscopy. The images were recor...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-007-9187-x
更新日期：2009-02-01 00:00:00
abstract：PURPOSE:Recently, the vasodilator relaxin 2 has been introduced as a treatment for acute heart failure. However, its role on vessels of the eye and intraocular pressure (IOP) remains unclear though it has been hypothesized to induce a decrease IOP after intramuscular injection in humans. We aimed to test whether the ho...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-018-0884-4
更新日期：2019-04-01 00:00:00
abstract：PURPOSE:To evaluate circular neodymium: YAG laser incision in cases of anterior capsular contraction syndrome (ACCS) by comparing it to radial Neodymium: YAG laser incision as for efficacy and safety. SETTINGS:Ophthalmology Department, Benha University Hospitals. METHODS:The study was done on 74 eyes of 66 patients w...
journal_title：International ophthalmology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10792-019-01094-9
更新日期：2019-11-01 00:00:00
abstract：PURPOSE:To assess variability in the coefficient of variation (COV) in cell area estimates when using different numbers of cells for endothelial morphometry. METHODS:Using non-contact specular microscopy images of the corneal endothelium, 4 sets of 20 cases were selected that included 200 cells and had overall (global...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-017-0765-2
更新日期：2018-12-01 00:00:00
abstract：:To report a case of bilateral granulomatous post-streptococcal syndrome uveitis in association with reactive arthritis as manifestation of post-streptococcal syndrome. To our knowledge, this could represent the first reported case in the literature. A 9-year-old girl, with no past ocular history, presented with a 5-da...
journal_title：International ophthalmology
pub_type： 杂志文章
doi：10.1007/s10792-012-9626-1
更新日期：2015-10-01 00:00:00
