Abstract：
:Load-bearing tissues owe their mechanical strength to their highly anisotropic collagenous structure. To date attempts to engineer mechanically strong connective tissue have failed, mainly due to a lack of ability to reproduce the native collagen organization in constructs synthesized by cultured cells in vitro. The ability to influence the orientation of self-assembling collagen molecules to produce highly anisotropic structures has applications ranging from de novo engineering of complex tissues to the production of organized scaffolds for cell culture contact guidance. In this investigation we have used the simple technique of spin-coating to produce highly aligned arrays of collagen fibrils. By a simple modification of the method we have also successfully produced orthogonal collagen lamellae. Alternating collagen lamellae are frequently seen in load-bearing tissues such as cornea, annulus fibrosus, and cortical bone. Culturing of corneal fibroblasts on aligned collagen shows that the cells adopt the organization of fibrils. In this investigation we observed the reversal of fibrillar growth direction or "hook" formation similar to that seen previously in a microfluidic shear flow chamber. Although the results of this investigation clearly show that it is possible to produce small areas (1cm(2)) of collagen fibrils with enough alignment to guide fibroblasts, there is evidence that thin film instabilities are likely to be a significant barrier to producing organized collagen fibrils over larger areas. Successful application of this method to produce highly controlled and organized collagenous structures will require the development of techniques to control thin film instability and will be the subject of future work.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Saeidi N,Sander EA,Zareian R,Ruberti JWdoi
10.1016/j.actbio.2011.02.038subject
Has Abstractpub_date
2011-06-01 00:00:00pages
2437-47issue
6eissn
1742-7061issn
1878-7568pii
S1742-7061(11)00108-5journal_volume
7pub_type
杂志文章abstract：:The main objective of this study was to investigate calcium phosphate (CaP) coatings on Ti6Al4V substrates by using the biomimetic technique. To this purpose, a new solution was developed to coat CaP on Ti6Al4V alloy substrates. The newly formulated body fluid (Lac-SBF) contained appropriate amounts of sodium lactate ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.013
更新日期：2010-06-01 00:00:00
abstract：:The management of diabetic wounds remains a major therapeutic challenge in clinics. Herein, we report a personalized treatment using 3D scaffolds consisting of radially or vertically aligned nanofibers in combination with bone marrow mesenchymal stem cells (BMSCs). The 3D scaffolds have customizable sizes, depths, and...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.03.035
更新日期：2020-05-01 00:00:00
abstract：:Bone morphogenetic protein-2 (BMP-2) is known to enhance fracture healing when delivered via a bovine collagen sponge. However, collagen rapidly releases BMP-2 with a high burst phase that is followed by a low sustained phase. As a result, supra-physiological doses of BMP-2 are often required to successfully treat bon...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.07.008
更新日期：2013-11-01 00:00:00
abstract：:Perinatal tissues are an abundant source of human extracellular matrix proteins, growth factors and stem cells with proved potential use in a wide range of therapeutic applications. Due to their placental origin, these tissues possess unique biological properties, including being angiogenic, anti-inflammatory, anti-fi...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2020.04.035
更新日期：2020-07-01 00:00:00
abstract：UNLABELLED:Humans make high levels of antibody to carbohydrates with terminal galactose α 1,3 galactose (Gal) modifications. This Gal antigen is widely expressed in other mammals and is present on an array of current animal derived biomedical devices including bioprosthetic heart valves. There is growing interest in us...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.06.007
更新日期：2016-09-01 00:00:00
abstract：:Three protease-resistant bioorganic 304 stainless steel surfaces were created through the reaction of synthetic peptides consisting of the D-enantiomeric isomer (D-K122-4), the retro-inverso D-enantiomeric isomer (RI-K122-4), and a combination of the two peptides (D+RI) of the Pseudomonas aeruginosa PilA receptor bind...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.11.026
更新日期：2013-04-01 00:00:00
abstract：:Tissue growth in bioscaffolds is influenced significantly by pore geometry, but how this geometric dependence emerges from dynamic cellular processes such as cell proliferation and cell migration remains poorly understood. Here we investigate the influence of pore size on the time required to bridge pores in thin 3D-p...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.07.010
更新日期：2020-09-15 00:00:00
abstract：:A rapidly expanding literature broadly documents the impact of biophysical cues on cellular behaviors. In spite of increasing research efforts in this field, the underlying signaling processes are poorly understood. One of the candidate molecules for being involved in mechanotransduction is focal adhesion kinase (FAK)...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.07.004
更新日期：2012-12-01 00:00:00
abstract：:We previously reported that PTH(1-34) inhibits the terminal differentiation of articular chondrocytes and, in turn, suppresses the progression of osteoarthritis (OA). However, this treatment requires an injection of PTH(1-34) once every 3 days over the treatment period. In this study, we studied the effect of sustaine...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.03.015
更新日期：2012-07-01 00:00:00
abstract：:Several studies have reported the benefits of mesenchymal stem cells (MSCs) for bone tissue engineering. However, vascularization remains one of the main obstacles that must be overcome to reconstruct large bone defects. In vitro prevascularization of the three-dimensional (3-D) constructs using co-cultures of human p...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.05.025
更新日期：2013-09-01 00:00:00
abstract：:Thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. This work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. Boophilin, a direct ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.07.020
更新日期：2012-11-01 00:00:00
abstract：UNLABELLED:Recent studies reveal many biomaterial vaccine carriers are able to activate immunostimulatory pathways, even in the absence of other immune signals. How the changing properties of polymers during biodegradation impact this intrinsic immunogenicity is not well studied, yet this information could contribute t...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.12.026
更新日期：2016-03-01 00:00:00
abstract：:In this work, plasma-sprayed nanostructured zirconia coatings stabilized with 3mol.% yttria (3Y-TZP) were deposited on Ti substrates. The microstructure and phase composition of coatings were characterized using scanning electron microscopy and X-ray diffraction. The in vitro bioactivity of coatings was evaluated by e...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.01.023
更新日期：2009-07-01 00:00:00
abstract：:Self-assembled nanovehicles of chemotherapy drug with photothermal agent are regarded as intriguing chemo-photothermal therapy nanoplatform. However, most of the drugs and photothermal agents have poor water solubility and poor interactions to drive the formation of self-assembled nanovehicles, which is a bottleneck o...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.11.049
更新日期：2019-01-15 00:00:00
abstract：:In this research, the flexural performance of hedgehog spines is investigated in four ways. First, X-ray micro-computed tomography (μCT) is employed to analyze the complex internal architecture of hedgehog spines. μCT images reveal distinct structural morphology, characterized by longitudinal stringers and transverse ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.036
更新日期：2019-08-01 00:00:00
abstract：:Recently, a significant amount of effort has been dedicated to understanding factors that influence the functionality of bio-electronic sensors and to development of novel coating technologies for modifying biosensor surfaces. Due to its well-known biocompatibility, alginate hydrogel (HG) has been used as a coating ma...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.07.034
更新日期：2010-01-01 00:00:00
abstract：:In the present study, the density functional theory implemented in the Vienna Ab-initio Simulation Package has been used to investigate the alloying effects of different elements of calcium, zinc, yttrium, aluminum and iron when introduced in the Mg crystal lattice. In particular, studies have been conducted to invest...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.08.016
更新日期：2010-05-01 00:00:00
abstract：:Antimicrobial peptides (AMP) are powerful components of the innate immune system, as they display wide activity spectrum and low tendency to induce pathogen resistance. Hence, the development of AMP-based coatings is a very promising strategy to prevent biomaterials-associated infections. This work aims to investigate...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.12.001
更新日期：2019-01-15 00:00:00
abstract：:In the U.S., 30% of adults suffer joint pain, most commonly in the knee, which severely limits mobility and is often attributed to injury of cartilage and underlying bone in the joint. Current treatment methods such as microfracture result in less resilient fibrocartilage with eventual failure; autografting can cause ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.10.016
更新日期：2018-12-01 00:00:00
abstract：:Three-dimensional (3D) porous zinc (Zn) with a moderate degradation rate is a promising candidate for biodegradable bone scaffolds. However, fabrication of such scaffolds with adequate mechanical properties remains a challenge. Moreover, the composition, crystallography and microstructure of the in vivo degradation pr...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.11.032
更新日期：2021-02-01 00:00:00
abstract：UNLABELLED:Cardiovascular disease is the leading cause of death in the world. In this study, coaxial electrospinning is employed to fabricate fibers in a core-shell structure with polyvinyl alcohol (PVA) in the core and gelatin in the shell for evaluation as a potential vascular tissue engineering construct. PVA, a syn...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.08.044
更新日期：2015-11-01 00:00:00
abstract：:Cartilaginous tissues engineered using mesenchymal stem cells (MSCs) have been shown to generate bone in vivo by executing an endochondral programme. This may hinder the use of MSCs for articular cartilage regeneration, but opens the possibility of using engineered cartilaginous tissues for large bone defect repair. H...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.11.031
更新日期：2015-02-01 00:00:00
abstract：:Corneal disease is a major cause of blindness and keratoplasty is an effective treatment method. However, clinical treatment is limited due to a severe shortage of high-quality allogeneic corneal tissues and the bacterial infection after corneal transplantation. In this study, we develop a novel artificial and antibac...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.08.033
更新日期：2014-01-01 00:00:00
abstract：:Photochemical internalisation (PCI) is a method for enhancing delivery of drugs to their intracellular target sites of action. In this study we investigated the efficacy of PCI using a porphyrin photosensitiser and a cytotoxic agent on spheroid and non-spheroid compressed collagen 3D constructs of ovarian cancer versu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.09.041
更新日期：2018-11-01 00:00:00
abstract：:Decellularized extracellular matrix (ECM) derived from stem cells has been shown as a promising biomaterial for bone regeneration because of the promotion effect on osteogenesis in mesenchymal stem cells (MSCs). However, bone regeneration is also influenced by bone resorption and little is known about the effect of ce...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.03.003
更新日期：2018-04-15 00:00:00
abstract：:A major problem with transcutaneous osseointegrated implants is infection, mainly due to improper closure of the implant-skin interface. Therefore, the design of transcutaneous osseointegrated devices that better promote skin growth around these exit sites needs to be examined and, if successful, would clearly limit i...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.016
更新日期：2010-06-01 00:00:00
abstract：:Three-dimensional (3D) tissue-engineered in vitro models, particularly multicellular spheroids and organoids, have become important tools to explore disease progression and guide the development of novel therapeutic strategies. These avascular constructs are particularly powerful in oncological research due to their a...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.09.042
更新日期：2020-11-01 00:00:00
abstract：:Bone replacement using synthetic and degradable materials is desirable in various clinical conditions. Most applied commercial materials are based on hydroxyapatite, which is not chemically degradable under physiological conditions. Here we report the effect of a long-term intramuscular implantation regime on the diss...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.05.022
更新日期：2011-09-01 00:00:00
abstract：:There is increasing interest in the development of new tissue engineering strategies to deliver cells and bioactive agents encapsulated in a biodegradable matrix through minimally invasive procedures. The present work proposes to combine chitosan-beta-glycerophosphate salt formulations with bioactive glass nanoparticl...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.08.006
更新日期：2009-01-01 00:00:00
abstract：:One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the he...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.10.018
更新日期：2017-01-15 00:00:00
