Abstract:
:One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the health authorities. This paper reports an innovative approach to coating surfaces using a natural extracellular matrix (ECM) extracted from the Wharton's jelly (WJ) of the umbilical cord (referred to as WJ-ECM). We have shown by atomic force microscopy (AFM), that the deposition of WJ-ECM on surfaces is homogenous with a controllable thickness, and that this easily-prepared coating is appropriate for both the adhesion and proliferation of human mesenchymal stem cells and mature endothelial cells. Furthermore, under physiological shear stress conditions, a larger number of cells remained adhered to WJ-ECM than to a conventional coating such as collagen - a result supported by the higher expression of both integrins α2 and β1 in cells cultured on WJ-ECM. Our data clearly show that Wharton's jelly is a highly promising coating for the design of human biocompatible surfaces in tissue engineering as well as in regenerative medicine. STATEMENT OF SIGNIFICANCE:Discovery and design of biomaterial surface are a hot spot in the tissue engineering field. Natural matrix is preferred to mimic native cell microenvironment but its use is limited due to poor resource availability. Moreover, current studies often use single or several components of natural polymers, which is not the case in human body. This paper reports a natural extracellular matrix with full components derived from healthy human tissue: Wharton's jelly of umbilical cord. Reconstituting this matrix as a culture surface, our easily-prepared coating provides superior biocompatibility for stem and mature cells. Furthermore, we observed improved cell performance on this coating under both static and dynamic condition. This novel human derived ECM would be a promising choice for regenerative medicine.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Dan P,Velot É,Francius G,Menu P,Decot Vdoi
10.1016/j.actbio.2016.10.018subject
Has Abstractpub_date
2017-01-15 00:00:00pages
227-237eissn
1742-7061issn
1878-7568pii
S1742-7061(16)30545-1journal_volume
48pub_type
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2013.04.014
更新日期:2013-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Acta biomaterialia
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更新日期:2010-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2016.09.026
更新日期:2016-12-01 00:00:00
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doi:10.1016/j.actbio.2018.02.026
更新日期:2018-04-15 00:00:00
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更新日期:2015-10-01 00:00:00
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更新日期:2016-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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