Abstract：
:Challenges remain in the generation of heterogeneous tissues and the repairing of interfacial tissue between soft and hard tissues. The development of tissue engineering scaffolds with gradients in composition, structure, mechanical and chemical properties is essential to modulate cellular behaviors in a graded way and potentially support the growth of functionally graded tissues. Integrated with the three-dimensional (3-D) nanofibrous skeletal structure of native extracellular matrix, electrospun fibers with gradients in amino groups were generated in the current study through an aminolysis process by using a microinfusion pump. Gelatin grafts were constructed to create fibrous scaffolds with gradients in hydroxyapatite (HA) contents, crystal size and mechanical properties through in situ mineralization. Plasmid DNA (pDNA) was included during the mineralization process, and gradations in pDNA loading contents were created on fibrous scaffolds on the basis of HA gradients. Obvious gradients in cell density, osteoblastic differentiation and collagen deposition were demonstrated along the long axis of fibrous mats after cell seeding. Gradients in the amount of pDNA released and the expression of target proteins were indicated on the fibrous mats, which offered a temporally and spatially controlled delivery of growth factors in scaffolds. The creation of gradient futures on 3-D fibrous scaffolds may provide physical, chemical and biological cues and result in efficient regeneration of tissues with spatial distributions of the cell proliferation, differentiation, and matrix secretion.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Zou B,Liu Y,Luo X,Chen F,Guo X,Li Xdoi
10.1016/j.actbio.2012.01.003subject
Has Abstractpub_date
2012-04-01 00:00:00pages
1576-85issue
4eissn
1742-7061issn
1878-7568pii
S1742-7061(12)00010-4journal_volume
8pub_type
杂志文章abstract：:Thin films of amorphous carbon and amorphous, graphitic and fullerene-like carbon nitride were deposited by reactive magnetron sputtering and optically characterized with spectroscopic ellipsometry. Complementary studies using scanning electron microscopy and atomic force microscopy were performed. The films were expo...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.10.024
更新日期：2011-03-01 00:00:00
abstract：:Serum albumin was conjugated to poly-(ethylene glycol) (PEG) and cross-linked to form mono-PEGylated albumin hydrogels. These hydrogels were used as a basis for drug carrying tissue engineering scaffold materials, based on the natural affinity of various drugs and compounds for the tethered albumin in the polymer netw...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.07.017
更新日期：2011-01-01 00:00:00
abstract：:Introducing neural stem/progenitor cells (NS/PCs) for repairing facial nerve injuries could be an alternative strategy for nerve gap reconstruction. However, the lack of success associated with current methods of applying NS/PCs to neurological disease is due to poor engraftment following transplantation into the host...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.11.064
更新日期：2017-03-01 00:00:00
abstract：:The boundary layers coating articular cartilage in synovial joints constitute unique biomaterials, providing lubricity at levels unmatched by any human-made materials. The underlying molecular mechanism of this lubricity, essential to joint function, is not well understood. Here we study the interactions between surfa...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.06.043
更新日期：2017-09-01 00:00:00
abstract：:Predictive in vitro models are valuable alternatives to animal experiments for evaluating the transport of molecules and (nano)particles across biological barriers. In this work, an improved triple co-culture of air-blood barrier was set-up, being exclusively constituted by human cell lines that allowed to perform exp...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.037
更新日期：2019-06-01 00:00:00
abstract：:Despite progress in use of decellularized lung scaffolds in ex vivo lung bioengineering schemes, including use of gels and other materials derived from the scaffolds, the detailed composition and functional role of extracellular matrix (ECM) proteoglycans (PGs) and their glycosaminoglycan (GAG) chains remaining in dec...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.11.029
更新日期：2020-01-15 00:00:00
abstract：UNLABELLED:Antifouling polymers have wide applications in biomedical engineering and marine industry. Recently, zwitterionic materials have been reported as promising candidates for antifouling applications, while strong hydration is believed to be the key antifouling mechanism. Zwitterionic materials can be designed w...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2016.02.030
更新日期：2016-08-01 00:00:00
abstract：:One of the outstanding goals in tissue engineering is to develop a natural coating surface which is easy to manipulate, effective for cell adhesion and fully biocompatible. The ideal surface would be derived from human tissue, perfectly controllable, and pathogen-free, thereby satisfying all of the standards of the he...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.10.018
更新日期：2017-01-15 00:00:00
abstract：:The development of an in situ formed pH-responsive theranostic nanocomposite for anticancer drug delivery and computed tomography (CT) imaging was reported. β-cyclodextrin-{poly(lactide)-poly(2-(dimethylamino) ethyl methacrylate)-poly[oligo(2-ethyl-2-oxazoline)methacrylate]}21 [β-CD-(PLA-PDMAEMA-PEtOxMA)21] unimolecul...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.06.003
更新日期：2017-08-01 00:00:00
abstract：:This work focuses on the synthesis and characterization of a novel bioresorbable glass ceramic phosphate-based material (GC-ICEL). More specifically, its solubility in different aqueous media (water, Tris-HCl and acellular simulated body fluid) and the response of human stromal cells cultured on it were investigated. ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.07.017
更新日期：2010-02-01 00:00:00
abstract：:Human enamel has been found to be a coating with excellent mechanical performance, and has undergone extensive investigation and discussion. However, most of the reported studies consider the enamel as a homogeneous anisotropic biocomposite. The current study illustrated the graded properties of the biocomposite from ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.12.029
更新日期：2013-05-01 00:00:00
abstract：:The objective of this study was to develop and characterize a novel intravaginal membrane platform for pH-triggered release of nanoparticles (NPs), which is essential for efficient intravaginal delivery of certain effective but acid-labile therapeutic agents for sexually transmitted infections, such as small interferi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.10.003
更新日期：2018-12-01 00:00:00
abstract：:The quest for predictive tumor markers for osteosarcoma (OS) has not well progressed over the last two decades due to a lack of preclinical models. The aim of this study was to investigate if microenvironmental modifications in an original humanized in vivo model alter the expression of OS tumor markers. Human bone mi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.02.051
更新日期：2019-04-15 00:00:00
abstract：UNLABELLED:Epithelial cellular heterogeneity has been observed in pathological tissues with abnormal matrix stiffness and cells cultured on rigid substrates. However, it remains unclear how matrix stiffness influences cellular heterogeneity formation in multi-cellular population. Here, we demonstrated that cellular het...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.05.025
更新日期：2016-09-01 00:00:00
abstract：:Articular cartilage is a remarkable tissue whose sophisticated composition and architecture allow it to withstand complex stresses within the joint. Once injured, cartilage lacks the capacity to self-repair, and injuries often progress to joint wide osteoarthritis (OA) resulting in debilitating pain and loss of mobili...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.01.061
更新日期：2019-07-15 00:00:00
abstract：:Tissue engineered vascular grafts (TEVGs) represent a promising therapeutic option for emergency vascular intervention. Although the application of small-diameter TEVGs using patient-specific primary endothelial cells (ECs) to prevent thrombosis and occlusion prior to implantation could be hindered by the long time co...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.11.007
更新日期：2021-01-01 00:00:00
abstract：:CRISPR gene editing technology is strategically foreseen to control diseases by correcting underlying aberrant genetic sequences. In order to overcome drawbacks associated with viral vectors, the establishment of an effective non-viral CRISPR delivery vehicle has become an important goal for nanomaterial scientists. H...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.07.027
更新日期：2020-09-15 00:00:00
abstract：:Incorporation of hydroxyapatite (HA) within a degradable polymeric scaffold may provide a favorable synthetic microenvironment that more closely mimics natural bone tissue physiology. Both incorporation of HA nanoparticles and alterations of the paracrine cell-cell signaling distance may affect the intercellular signa...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.11.007
更新日期：2011-03-01 00:00:00
abstract：OBJECTIVE:An underlying abnormality in collagen turnover is implied in the occurrence of complications and recurrences after mesh augmented pelvic floor repair surgeries. Ascorbic acid is a potent stimulant of collagen synthesis. The aim of this study is to produce ascorbic acid releasing poly-lactic acid (PLA) scaffol...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.10.019
更新日期：2016-01-01 00:00:00
abstract：UNLABELLED:Porous titanium implants are widely used in dental, orthopaedic and otorhinolaryngology fields to improve implant integration to host tissue. A possible step further to improve the integration with the host is the incorporation of autologous cells in porous titanium structures via cell-laden hydrogels. Fast ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.01.023
更新日期：2016-03-01 00:00:00
abstract：:Beta-tricalcium phosphate (beta-TCP) is a suitable biomaterial in oral and maxillofacial surgery since it can induce a rapid proliferation of woven bone. Granules, prepared by the polyurethane foam method, were implanted in critical size defects performed in the femoral condyles of New Zealand rabbits. Animals were st...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.01.042
更新日期：2010-08-01 00:00:00
abstract：:A novel bactericidal material comprising rod-shaped core-shell-shell Au-Ag-Au nanorods is constructed as a nanoheater in the near-infrared (NIR) region. The outer Au shell melts under laser irradiation and results in exposure of the inner Ag shell, facilitating the controlled release of the antibacterial Ag shell/laye...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.09.005
更新日期：2015-01-01 00:00:00
abstract：:Fluorescence resonance energy transfer (FRET) was employed to monitor the molecular dissociation of a chitosan/DNA complex with different molecular weights of chitosan. Chitosan with different molecular weights was complexed with plasmid DNA and the complex formation was monitored using dynamic light scattering and a ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.01.010
更新日期：2008-07-01 00:00:00
abstract：:Our objectives were to correlate the mineral density (MD) and elastic modulus (E) of natural white spot lesions (WSLs) and compare them with analytical and numerical models. Five natural WSLs from four extracted sound premolar teeth were scanned at a voxel size of 7.6μm using a desktop X-ray microtomography (XRMT) sys...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.06.028
更新日期：2010-12-01 00:00:00
abstract：:Much work has been devoted to analysing thermodynamic models for solid dispersions with a view to identifying regions in the phase diagram where amorphous phase separation or drug recrystallization can occur. However, detailed partial differential equation non-equilibrium models that track the evolution of solid dispe...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.06.009
更新日期：2019-08-01 00:00:00
abstract：:Avidin was covalently conjugated to the surface of calcium phosphate nanoparticles, coated with a thin silica shell and terminated by sulfhydryl groups (diameter of the solid core about 50nm), with a bifunctional crosslinker connecting the amino groups of avidin to the sulfhydryl group on the nanoparticle surface. Thi...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.05.049
更新日期：2017-07-15 00:00:00
abstract：:Hydrogels have been recognized as crucial biomaterials in the field of tissue engineering, regenerative medicine, and drug delivery applications due to their specific characteristics. These biomaterials benefit from retaining a large amount of water, effective mass transfer, similarity to natural tissues and the abili...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2017.07.028
更新日期：2017-10-15 00:00:00
abstract：:This study deals with cytotoxicity assays performed on an array of commercially manufactured inorganic nanoparticulate materials, including Ag, TiO(2), Fe(2)O(3), Al(2)O(3), ZrO(2), Si(3)N(4), naturally occurring mineral chrysotile asbestos and carbonaceous nanoparticulate materials such as multiwall carbon nanotube a...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.11.004
更新日期：2007-05-01 00:00:00
abstract：:The understanding of corrosion processes of metal implants in the human body is a key problem in modern biomaterial science. Because of the complicated and adjustable in vivo environment, in vitro experiments require the analysis of various physiological corrosion media to elucidate the underlying mechanism of "biolog...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.03.004
更新日期：2011-06-01 00:00:00
abstract：:Breast implant use has tripled in the last decade with over 320,000 breast implant based reconstructions and augmentations performed in the US per annum. Unfortunately a considerable number of women will experience capsular contracture, the irrepressible and disfiguring, tightening and hardening of the fibrous capsule...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.11.052
更新日期：2017-02-01 00:00:00
