Abstract：
:Antimicrobial peptides (AMP) are powerful components of the innate immune system, as they display wide activity spectrum and low tendency to induce pathogen resistance. Hence, the development of AMP-based coatings is a very promising strategy to prevent biomaterials-associated infections. This work aims to investigate if Dhvar-5-chitosan conjugates, previously synthesized by us via azide-alkyne "click" reaction, can be applied as antimicrobial coatings. Ultrathin coatings were prepared by spin coater after dissolving Dhvar-5-chitosan conjugate powder in aqueous acetic acid. Peptide orientation and exposure from the surface was confirmed by ellipsometry and contact angle measurements. Bactericidal activity was evaluated against Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, the most prevalent pathogens in implant-associated infections. Results showed that Dhvar-5-chitosan coatings displayed bactericidal effect. Moreover, since Dhvar-5 has head-to-tail amphipathicity, it was clear that the bactericidal potency was dependent on which domain of the peptide (cationic or hydrophobic) was exposed. In this context, Dhvar-5 immobilized through its C-terminus (exposing its hydrophobic end) presented higher antimicrobial activity against Gram-positive bacteria and reduced adhesion of Gram-negative bacteria. This orientation-dependent antimicrobial activity was further corroborated by the anti-biofilm assay, as covalent immobilization of Dhvar-5 through its C-terminus provided anti-biofilm properties to the chitosan thin film. Immobilization of Dhvar-5 showed no cytotoxic effect against HFF-1 cells, as both metabolic activity and cell morphology were similar to control. In conclusion, Dhvar-5-chitosan coatings are promising antimicrobial surfaces without cytotoxic effects against human cells. STATEMENT OF SIGNIFICANCE: AMP-tethering onto ground biomaterial is still a poorly explored strategy in research. In this work, AMP-tethered ground chitosan is used to produce highly antibacterial ultrathin films. Powdered AMP-tethered chitosan appears as an alternative solution for antimicrobial devices production, as it is suitable for large scale production, being easier to handle for fabrication of different coatings and materials with antimicrobial properties and without inducing toxicity.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Barbosa M,Costa F,Monteiro C,Duarte F,Martins MCL,Gomes Pdoi
10.1016/j.actbio.2018.12.001subject
Has Abstractpub_date
2019-01-15 00:00:00pages
242-256eissn
1742-7061issn
1878-7568pii
S1742-7061(18)30726-8journal_volume
84pub_type
杂志文章abstract：:The aim of this work was to study the phase transformation during the setting reaction of two calcium phosphate bone cements based on either alpha tricalcium phosphate (alpha-TCP) or tetracalcium phosphate (TetCP) initial solid phase, and a magnesium carbonate-phosphoric acid solution as the hardening liquid. Low mole...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.01.006
更新日期：2008-07-01 00:00:00
abstract：:Highly porous titanium with aligned large pores up to 500 μm in size, which is suitable for scaffold applications, was successfully fabricated using the reverse freeze casting method. In this process we have newly developed, the Ti powders migrated spontaneously along the pre-aligned camphene boundaries at a temperatu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.03.020
更新日期：2012-07-01 00:00:00
abstract：:The influence of ionic substituents in calcium phosphates intended for bone and tooth replacement biomedical applications is an important research topic, owing to the essential roles played by trace elements in biological processes. The present study investigates the mechanical and biological evaluation of ionic doped...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.12.009
更新日期：2011-04-01 00:00:00
abstract：:Glass-ceramic macroporous scaffolds for tissue engineering have been developed using a polyurethane sponge template and bioactive glass powders. The starting glass (CEL2) belongs to the system SiO(2)-P(2)O(5)-CaO-MgO-Na(2)O-K(2)O and has been synthesised by a conventional melting-quenching route. A slurry of CEL2 powd...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.07.012
更新日期：2007-03-01 00:00:00
abstract：:Serum albumin was conjugated to poly-(ethylene glycol) (PEG) and cross-linked to form mono-PEGylated albumin hydrogels. These hydrogels were used as a basis for drug carrying tissue engineering scaffold materials, based on the natural affinity of various drugs and compounds for the tethered albumin in the polymer netw...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.07.017
更新日期：2011-01-01 00:00:00
abstract：:The deposition of fibronectin (FN) from saline buffer-based cryogenic targets by matrix-assisted pulsed laser evaporation (MAPLE) onto silicon substrates is reported. A uniform distribution of FN was revealed by Ponceau staining after control experiments on nitrocellulose paper. Well-organized particulates with height...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2011.06.016
更新日期：2011-10-01 00:00:00
abstract：:Tilapia fish-scale type I atelocollagen hydrogels with aligned fibril structures were fabricated under a strong magnetic field of 6 or 12 T using two different methods. In the first method, a solution of acid-soluble collagen was neutralized with phosphate buffer saline and maintained in the magnetic field at 28°C for...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.09.014
更新日期：2011-02-01 00:00:00
abstract：:Peripheral nerve fibrosis and painful adhesions are common, recurring pathological sequelae following injury. In this study, vital human amnion (hAM), an increasingly interesting biomaterial for regenerative medicine, was investigated as a novel therapy. hAM was first analyzed in vitro regarding its anti-adhesive char...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.11.042
更新日期：2018-01-15 00:00:00
abstract：:α-Galactosylceramide (GalCer) is a glycolipid widely known as an activator of Natural killer T (NKT) cells, constituting a promising adjuvant against cancer, including melanoma. However, limited clinical outcomes have been obtained so far. This study evaluated the synergy between GalCer and major histocompatibility co...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.06.029
更新日期：2018-08-01 00:00:00
abstract：:Thin films of amorphous carbon and amorphous, graphitic and fullerene-like carbon nitride were deposited by reactive magnetron sputtering and optically characterized with spectroscopic ellipsometry. Complementary studies using scanning electron microscopy and atomic force microscopy were performed. The films were expo...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.10.024
更新日期：2011-03-01 00:00:00
abstract：:Olfaction is normally taken for granted in our lives, not only assisting us to escape from dangers, but also increasing our quality of life. Although olfactory neuroepithelium (ON) can reconstitute its olfactory receptor neurons (ORNs) after injury, no adequate treatment for olfactory loss has yet emerged. The present...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.08.022
更新日期：2019-10-01 00:00:00
abstract：:Recent phase 1 trials of embryonic stem cell and induced pluripotent stem cell (iPSCs) derived RPE transplants for the treatment of macular degeneration have demonstrated the relative safety of this process. However, there is concern over clumping, thickening, folding, and wrinkling of the transplanted RPE. To deliver...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.11.058
更新日期：2018-02-01 00:00:00
abstract：:Abnormal wound healing caused by the over-expression of collagen and fibronectin leads to fibrosis, the major complication of all treatment modalities. A three-layer nanofiber scaffold was designed, optimized, and fabricated. This scaffold comprised two supportive polycaprolactone (PCL)-chitosan layers on the sides an...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.06.031
更新日期：2020-09-01 00:00:00
abstract：UNLABELLED:Recently, we reported on a series of short amphipathic α-helical peptides, comprising the backbone sequence (LLKK)2, with the ability to kill susceptible and drug-resistant Mycobacterium tuberculosis. In this study, the effect of key physicochemical parameters including hydrophobicity and helicity of α-helic...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2015.09.015
更新日期：2015-12-01 00:00:00
abstract：:In the present work we have revisited the application of quantitative ultrasound imaging (QUI) to cellular hydrogels, by using the reference phantom method (RPM) in combination with a local attenuation compensation algorithm. The investigated biological samples consisted of cell-laden collagen hydrogels with PC12 neur...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2019.04.055
更新日期：2019-06-01 00:00:00
abstract：:Implant-derived material from metal-on-metal (MOM) hip arthroplasties may be responsible for an unexplained tissue inflammatory response. The chemical form of the metal species in the tissues is predominantly chromium (Cr), but the currently used techniques have not been able to determine whether this is Cr(III) phosp...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.06.006
更新日期：2010-11-01 00:00:00
abstract：:Myocardial infarction (MI) leads to the loss of cardiomyocytes, followed by left ventricular (LV) remodeling and cardiac dysfunction. The authors hypothesize that an elastic, biodegradable nanofibrous cardiac patch loaded with mesenchymal stem cells (MSC) could restrain LV remodeling and improve cardiac function after...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.02.030
更新日期：2014-06-01 00:00:00
abstract：:The combination of biodegradable metals and additive manufacturing (AM) leads to a revolutionary change of metal implants in many aspects including materials, design, manufacturing, and clinical applications. The AM of nondegradable metals such as titanium and CoCr alloys has proven to be a tremendous success in clini...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.04.046
更新日期：2019-10-15 00:00:00
abstract：:Differentiation of stem and progenitor cells routinely relies on the application of soluble growth factors, an approach that enables temporal control of cell fate but enables no spatial control of the differentiation process. Angiogenic progenitor cells derived from mouse embryonic stem cells (ESCs) were differentiate...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.005
更新日期：2010-06-01 00:00:00
abstract：:In this paper, we report X-ray diffraction investigations as well as Raman and solid-state (31)P and (23)Na magic angle spinning nuclear magnetic resonance (NMR) characterization of three series of calcium orthophosphates. The general formulae of the studied compounds are Ca(10.5-x/2)M(x)(PO(4))(7), where M=K or Na an...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.12.016
更新日期：2011-04-01 00:00:00
abstract：:Semiconducting nanoparticles, more commonly known as quantum dots, possess unique size and shape dependent optoelectronic properties. In recent years, these unique properties have attracted much attention in the biomedical field to enable real-time tissue imaging (bioimaging), diagnostics, single molecule probes, and ...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.05.022
更新日期：2019-08-01 00:00:00
abstract：:A chitosan oligosaccharide (CSO)-indomethacin (IDM) conjugate (CI) was synthesized to fabricate chemosensitizing nanoparticles (NPs) for tumor-targeted drug delivery. IDM was conjugated to a CSO backbone via amide bond formation, of which successful synthesis was confirmed by proton-nuclear magnetic resonance analyses...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.05.012
更新日期：2017-07-15 00:00:00
abstract：:Corneal collagen cross-linking (CXL) treatment can restore vision in patients suffering from keratoconus and corneal injury, by improving the mechanical properties of the cornea. The correlation between ultraviolet-A (UVA) irradiant energies of standard CXL (SCXL) and corneal visco-hyperelastic mechanical behavior rem...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.06.005
更新日期：2020-09-01 00:00:00
abstract：:For next-generation regenerative medicine and cancer therapy applications, the development of multifunctional biodegradable biomaterials with good antibacterial activity, biocompatibility and gene delivery capability is showing increased interests. As a natural cationic polypeptide, poly(ε-l-lysine) (EPL) has been as ...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.06.010
更新日期：2017-08-01 00:00:00
abstract：:Biomaterials made of biodegradable poly(α-hydroxyesters) such as poly(lactide-co-glycolide) (PLGA) are known to decrease the pH in the vicinity of the implants. Bioactive glass (BG) is being investigated as a counteracting agent buffering the acidic degradation products. However, in dentistry the question arises wheth...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.06.030
更新日期：2013-11-01 00:00:00
abstract：:Thin film nitinol has unique mechanical properties (e.g., superelasticity), excellent biocompatibility, and ultra-smooth surface, as well as shape memory behavior. All these features along with its low-profile physical dimension (i.e., a few micrometers thick) make this material an ideal candidate in developing low-pr...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2015.03.025
更新日期：2015-07-01 00:00:00
abstract：:This study investigated the surface characteristics, in vitro and in vivo biocompatibility of Ti-6Al-4V alloy implants incorporating strontium ions (Sr), produced by hydrothermal treatment using a Sr-containing solution, for future biomedical applications. The surface characteristics were evaluated by scanning electro...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.01.017
更新日期：2010-07-01 00:00:00
abstract：:The use of human mesenchymal stromal cells (hMSC) for treating diseased tissues with poor vascularization has received significant attention, but low cell survival has hampered its translation to the clinic. Bioglasses and glass-ceramics have also been suggested as therapeutic agents for stimulating angiogenesis in so...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.12.009
更新日期：2018-02-01 00:00:00
abstract：:Dentin contains 1-2μm diameter tubules extending from the pulp cavity to near the junction with enamel. Peritubular dentin (PTD) borders the tubule lumens and is surrounded by intertubular dentin (ITD). Differences in PTD and ITD composition and microstructure remain poorly understood. Here, a (∼200nm)(2), 10.1keV syn...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.05.027
更新日期：2014-09-01 00:00:00
abstract：:Recently, the progress in biomaterials for biomedical applications brings the focus of the research community toward nanomaterials. The nanofibrous matrices offer certain advantages (structural similarity to extracellular matrix, high surface area-to-volume ratio, increased elasticity, biostability, and strength) comp...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.07.047
更新日期：2018-09-15 00:00:00
