Abstract：
INTRODUCTION:Tazobactam-piperacillin (TZP) is a mixture of a broad-spectrum penicillin and an irreversible β-lactamase inhibitor. TZP is effective against Gram-negative bacteria that produce extended-spectrum β-lactamases, and it is used as a first-line or second-line drug to treat serious infections. METHODS:This study identified three TZP-resistant and two TZP-intermediate strains among 514 clinical isolates of Escherichia coli. RESULTS:These five isolates possessed one or more β-lactamase genes, blaTEM-1, blaCTX-M-2, blaCTX-M-14, and/or blaCMY-8. The expression levels of β-lactamase genes and acrAB genes in the strains were examined by using real-time reverse transcription PCR. The total enzymatic piperacillin-degrading activity in cells was determined. Two TZP-resistance mechanisms were identified: hyperproduction of TEM-1 in the two resistant strains; and simultaneous high production of β-lactamase and efflux pump AcrAB in the two TZP-intermediate isolates. The latter are an international high-risk clone O25b:H4-ST131-H30R. CONCLUSION:TZP resistance is still rare in clinical isolates of E. coli. However, resistance can develop on high production and/or combinations of known antimicrobial resistance mechanisms in different ways.
journal_name
Int J Antimicrob Agentsjournal_title
International journal of antimicrobial agentsauthors
Suzuki Y,Sato T,Fukushima Y,Nakajima C,Suzuki Y,Takahashi S,Yokota SIdoi
10.1016/j.ijantimicag.2020.105919subject
Has Abstractpub_date
2020-04-01 00:00:00pages
105919issue
4eissn
0924-8579issn
1872-7913pii
S0924-8579(20)30062-5journal_volume
55pub_type
杂志文章abstract：:The aim of this study was to screen lead compounds exhibiting potent in vitro antimicrobial activity against multidrug-resistant (MDR) Acinetobacter baumannii strains from a library of chemical compounds. In a high-throughput screening analysis of 7520 compounds representative of 340,000 small molecules, two 4H-4-oxoq...
journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2004.01.012
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
doi：10.1016/j.ijantimicag.2016.06.013
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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更新日期：2007-03-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
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更新日期：2020-11-29 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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更新日期：2013-07-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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更新日期：2019-12-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：International journal of antimicrobial agents
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journal_title：International journal of antimicrobial agents
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更新日期：1996-05-01 00:00:00
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journal_title：International journal of antimicrobial agents
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更新日期：2005-09-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章,评审
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更新日期：2009-07-01 00:00:00
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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journal_title：International journal of antimicrobial agents
pub_type： 杂志文章
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更新日期：2015-10-01 00:00:00
