Abstract:
:Neutrophils (PMN) from newborn calves generate significantly less superoxide anion (O2-) than do their adult counterparts after stimulation with direct protein kinase C agonists. To better understand this observation, we compared the activity and kinetics of NADPH oxidase in membrane fractions from phorbol 12-myristate 13-acetate-stimulated adult and newborn PMN. After phorbol 12-myristate 13-acetate stimulation, PMN were sonicated and the membranes assayed for O2- production with increasing concentrations of NADPH. O2- production was calculated 1 and 2 min after the beginning of the reaction. At all concentrations of NADPH used, there was no difference (p greater than 0.05) in O2- production between adult (n = 8) and newborn (n = 9) PMN membrane preparations. Enzyme kinetics calculations revealed no differences (p greater than 0.05) between age groups in Km and Vmax or in the velocity of the reactions. Determination of the protein content in the membrane pellet, however, showed that adult PMN yielded significantly (p less than 0.01) higher amounts of protein (2.82 +/- 0.14 mg/mL) than did newborn PMN (1.78 +/- 0.07 mg/mL). This difference could be partly attributed to cell size; flow cytometric analysis showed that newborn PMN had a significantly (p less than 0.01) smaller diameter (10.94 +/- 0.07 microns) than did adult PMN (11.65 +/- 0.06 microns), and calculated cell volume and surface area were also both significantly less (p less than 0.01) in newborn PMN.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Doré M,Slauson DO,Neilsen NRdoi
10.1203/00006450-199010000-00005subject
Has Abstractpub_date
1990-10-01 00:00:00pages
327-31issue
4eissn
0031-3998issn
1530-0447journal_volume
28pub_type
杂志文章abstract::Infants with polycythemia and hyperviscosity are known to have a reduced cerebral blood flow. Eight newborn lambs were studied to determine what effect the reduction in cerebral blood flow might have on the cerebral delivery and uptake of oxygen, glucose, lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate. Meas...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198803000-00019
更新日期:1988-03-01 00:00:00
abstract::Pesticides are chemicals that are designed specifically for the purpose of killing or suppressing another living organism. Human toxicity is possible with any pesticide, and a growing body of literature has investigated possible associations with neurodevelopmental disorders. Attention deficit disorder with or without...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/s41390-018-0200-z
更新日期:2019-01-01 00:00:00
abstract::Glutaryl-CoA dehydrogenase (GCDH) deficiency is a rare inborn disorder of L-lysine, L-hydroxylysine, and L-tryptophan metabolism complicated by striatal damage during acute encephalopathic crises. Three decades after its description, the natural history and how to treat this disorder are still incompletely understood....
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000219387.79887.86
更新日期:2006-06-01 00:00:00
abstract::This study reports the Hb, erythropoietin (Epo), and reticulocyte course at different postnatal ages (range, 8-83 d) of 56 blood samples from 30 newborns (range, one or two blood samples from each newborn) with erythroblastosis fetalis (18 intrauterine transfused (IUTd) and 12 non-IUTd). Hb, Epo, and reticulocyte valu...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199608000-00027
更新日期:1996-08-01 00:00:00
abstract::In spontaneously breathing newborn lambs, alveolar hypoxia increases lung microvascular pressure, which causes lung lymph flow to increase and the concentration of protein in lymph to decrease. To see if this response derives from hypoxia itself rather than from the change in breathing pattern that occurs during hypox...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198405000-00009
更新日期:1984-05-01 00:00:00
abstract::Epidermal growth factor (EGF), a potent mitogen present in milk, is postulated to play an important physiologic role in the ontogeny of the intestine. An artificial rat milk, free of EGF, was developed and fed to newborn rats in an effort to evaluate the physiologic role of EGF on intestinal glucose transport. Compari...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198704000-00017
更新日期:1987-04-01 00:00:00
abstract::We hypothesized that fetal pancreatic digestive enzymes play a role in the lung damage after meconium aspiration. We studied the effect of meconium on the A549 alveolar epithelial cell line. The exposure of the cells to 0.5 to 5% meconium resulted in significant disruption of connections between A549 cells and caused ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181ebd4c3
更新日期:2010-09-01 00:00:00
abstract:INTRODUCTION:Dietary fish oil (FO) was reported to lower fecal fat excretion in a weanling rat model of short bowel syndrome (SBS) after ileocecal resection (ICR), and to induce changes in secretion and synthesis of bile acid (BA) in adults. We hypothesized that dietary FO, as compared with corn oil (CO), increases int...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.41
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:Early recognition of patients at risk for sepsis is paramount to improve clinical outcomes. We hypothesized that subtle signatures of illness are present in physiological and biochemical time series of pediatric-intensive care unit (PICU) patients in the early stages of sepsis. METHODS:We developed multivar...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-019-0518-1
更新日期:2019-11-01 00:00:00
abstract::To determine whether human milk contained soluble receptors and cytokine antagonists that might contribute to its anti-inflammatory properties, ELISA and enzyme-amplified sensitivity immunoassay methods were used to quantitate soluble intercellular and vascular cell adhesion molecules, soluble E-selectin, soluble IL-6...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199612000-00011
更新日期:1996-12-01 00:00:00
abstract::Developmental differences in myocardial performance are known to exist. It is likely that the profile of protein isoforms present on the developing thin filament contributes to these observed differences. We have prepared thin filaments from developing and mature rat hearts by using an immunoprecipitation procedure de...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199109000-00006
更新日期:1991-09-01 00:00:00
abstract::Necrotizing enterocolitis (NEC) is a devastating intestinal disease of premature infants. Maternal milk has been suggested to be partially protective against NEC; however, the mechanisms of this protection are not defined. The aim of this study was to examine the effect(s) of artificial feeding of rat milk (RM)-versus...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000050657.56817.E0
更新日期:2003-03-01 00:00:00
abstract::Synchronized atrial contraction may be much more important in the newborn, who has a faster heart rate and a less compliant ventricle, than in the adult. We therefore investigated the extent by which synchronized atrioventricular contraction contributes to cardiac output and cardiac work in a neonatal circulation, and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199803000-00004
更新日期:1998-03-01 00:00:00
abstract::We have previously discovered that the adult Drosophila melanogaster is tolerant to a low O2 environment, withstanding hours of total O2 deprivation without showing any evidence of cell injury. Subsequently, our laboratory embarked on the study of hypoxia tolerance using a mutagenesis and overexpression screens to beg...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1203/PDR.0b013e3181b27275
更新日期:2009-09-01 00:00:00
abstract:BACKGROUND:Neonatal mortality and morbidity are increasingly recognized as important globally, but detailed estimates of neonatal morbidity from conditions and long-term consequences are yet to be published. METHODS:We describe the general methods for systematic reviews, meta-analyses, and modeling used in this supple...
journal_title:Pediatric research
pub_type: 历史文章,杂志文章
doi:10.1038/pr.2013.203
更新日期:2013-12-01 00:00:00
abstract::Heme oxygenase (HO), the rate-limiting enzyme in the formation of bilirubin, is expressed in the lung and may serve as an antioxidant. This enzyme results in the formation of antioxidant bile pigments and the degradation of pro-oxidant heme. We wanted to evaluate the differences in expression of HO-1, the inducible fo...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-200301000-00010
更新日期:2003-01-01 00:00:00
abstract::Rats were fed a 50% galactose diet during pregnancy and nursing, and the testes were later examined and hormone levels determined in male offspring. Exposure to galactose for various periods during pregnancy, throughout the entire gestation, or postnatally to nursing mother until pups were 5 wk of age produced no sign...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198404000-00008
更新日期:1984-04-01 00:00:00
abstract:BACKGROUND:Increasing numbers of preterm-born children survive nowadays, and improving long-term health and neurodevelopment is becoming more important. Early-life growth has been linked to neurodevelopmental outcomes. We aimed to study whether this association has changed with time. METHODS:We studied two cohorts of ...
journal_title:Pediatric research
pub_type: 杂志文章,多中心研究
doi:10.1038/s41390-018-0139-0
更新日期:2019-02-01 00:00:00
abstract::Factors affecting bone turnover in premature infants are not entirely clear but certainly are different from those influencing bones of adults and children. To identify fetal and maternal factors that might influence bone turnover, we prospectively studied 50 infants (30 preterm and 20 full-term) born at Ain Shams Uni...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000160591.70409.C8
更新日期:2005-05-01 00:00:00
abstract::Cerebral blood flow (CBF) was measured by means of Celabeled microspheres in infant (20-day-old) and adult (3-month-old) rats, anesthetised with Na-5-ethyl-5-(1-methylpropyl)2-thiobarbituric acid. Cerebral arteriovenous differences of acetoacetate, D-beta-hydroxybutyrate, glucose, lactate, and oxygen and brain DNA con...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197611000-00002
更新日期:1976-11-01 00:00:00
abstract::The absolute and the percentage plasma NEFA pattern of healthy children, Cystic Fibrosis (C.F.) patients and their parents have been determined (table I, III, V) and compared with those of age and sex matched control subjects (table II, Iv, V). There is a striking difference in the percentage plasma NEFA pattern of C....
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198009000-00015
更新日期:1980-09-01 00:00:00
abstract::Neurogenesis persists throughout life in the rodent subventricular zone (SVZ) and increases in the adult after brain injury. In this study, postnatal day 7 (P7) rats underwent right carotid artery ligation followed by 8% O2 exposure for 90 min, a lesioning protocol that resulted in ipsilateral forebrain hypoxic-ischem...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000179381.86809.02
更新日期:2005-09-01 00:00:00
abstract::The length of the Y chromosome was measured in 42 black patients with trisomy 21 (47,XY,+21) and a similar number of normal individuals of American black ancestry. The length of the Y was expressed as a function of Y/F ratio and arbitrarily classified into five groups using subjectively defined criteria as follows: ve...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198209000-00012
更新日期:1982-09-01 00:00:00
abstract::We have evaluated the effect of galanin (Gal), a newly identified hypothalamic peptide, on growth hormone (GH) secretion in 10 children with normal stature (NS), nine with constitutional growth delay (CGD), and five with isolated GH deficiency (IGHD). Gal was infused intravenously at a rate of 8 or 15 micrograms/kg/h....
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198910000-00006
更新日期:1989-10-01 00:00:00
abstract::In Chinese children with steroid-resistant nephrotic syndrome (SRNS), it was reported that NPHS2 mutation was detected in 4.3%, which was lower than that in Caucasians (10-30%). However, there were no data on WT1 mutation in nephrotic syndrome (NS), especially in early-onset NS of Chinese children. Thus, a study, whic...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181e4c9e3
更新日期:2010-08-01 00:00:00
abstract::This study was made to determine the life span of adult red cells transfused to early preterm infants. Nineteen very preterm infants (birth weight, 878.7 +/- 221 g; gestational age, 26.8 +/- 1.5 wk at birth) were sampled weekly after their last blood transfusion to determine the level (%) of fetal Hb in their circulat...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199707000-00002
更新日期:1997-07-01 00:00:00
abstract::Increased deposition of hyaluronan (HA) is part of the early response to fibrogenic stimulus in the lung exposed to bleomycin injury and has been associated with increased lung water in adult animals. Early respiratory distress syndrome (RDS) in premature infants is characterized by increased lung water, and late sequ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199402000-00024
更新日期:1994-02-01 00:00:00
abstract::We determined the inhibitory effects of concentrations of tin- and zinc protoporphyrin (1-100 microM) and mesoporphyrin (0.1-10 microM) on the in vitro heme oxygenase (HO) (E.C.1.14.99.3) activity in liver, spleen, and intestine 13,000 x g tissue supernatants from fasted adult male Wistar rats through measurement of c...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198910000-00015
更新日期:1989-10-01 00:00:00
abstract::Antigenic analysis of neuroblastoma cells was performed by autologous typing and indirect immunofluorescent test combined with absorption tests. Serum of a patient with neuroblastoma before treatment showed weak reactivity with cultured autologous neuroblastoma cells, while serum obtained from the same patient 3 month...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198610000-00001
更新日期:1986-10-01 00:00:00
abstract::Intracellular levels of cysteine (CSH) and reduced glutathione (GSH) in the kidney and liver of rats from the newborn to the adult period have been determined using a sensitive high performance liquid chromatography method. In the kidney, the intracellular level of free CSH increased 4-fold from 1 to 4 nmol/mg protein...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198711000-00025
更新日期:1987-11-01 00:00:00