Abstract:
:Neurogenesis persists throughout life in the rodent subventricular zone (SVZ) and increases in the adult after brain injury. In this study, postnatal day 7 (P7) rats underwent right carotid artery ligation followed by 8% O2 exposure for 90 min, a lesioning protocol that resulted in ipsilateral forebrain hypoxic-ischemic (HI) injury. The effects of HI injury on SVZ cell proliferation and neurogenesis were examined 1-3 wk later by morphometric measurement of dorsolateral SVZ size; by immunoassays to detect incorporation of bromodeoxyuridine (BrdU) in proliferating cells; and by immunoassays of doublecortin, a microtubule-associated protein expressed only by immature neurons. For determining the cell phenotypes of newly generated cells, tissue sections were double labeled with antibodies to BrdU and markers of mature neurons (neuronal nuclear protein), astrocytes (glial fibrillary acidic protein), or oligodendroglia (RIP). HI injury resulted in enlargement of the ipsilateral SVZ at P14-28 and a corresponding increase in BrdU cell numbers both in the ipsilateral SVZ and striatum at P21. HI injury also stimulated SVZ neurogenesis, based on increased doublecortin immunostaining in the SVZ ipsilateral to lesioning at P14-28. However, 4 wk after HI injury, in the lesioned striatum, although BrdU/glial fibrillary acidic protein and BrdU/RIP-labeled cells were identified, no BrdU/neuronal nuclear protein double-labeled cells were found. These results suggest that although acute neonatal HI injury stimulates SVZ proliferation and neurogenesis, there is inadequate trophic support for survival of newly generated neurons. Identification of the trophic factors that enhance maturation and survival of immature neurons could provide important clues for improving recovery after neonatal brain injury.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Ong J,Plane JM,Parent JM,Silverstein FSdoi
10.1203/01.PDR.0000179381.86809.02subject
Has Abstractpub_date
2005-09-01 00:00:00pages
600-6issue
3eissn
0031-3998issn
1530-0447pii
58/3/600journal_volume
58pub_type
杂志文章abstract::Completely penetrant mutations in the surfactant protein B gene (SFTPB) and >75% reduction of SFTPB expression disrupt pulmonary surfactant function and cause neonatal respiratory distress syndrome. To inform studies of genetic regulation of SFTPB expression, we created a catalogue of SFTPB variants by comprehensive r...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3180a03232
更新日期:2007-08-01 00:00:00
abstract::This study aimed to investigate 1) the plasma resistin concentration at birth, 2) the relationship of resistin with leptin and insulin, and 3) the association of resistin with anthropometric indexes in newborn infants. Blood samples for hormonal assay were obtained from preterm and term newborns within the first 2 h o...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000180556.76864.9A
更新日期:2005-10-01 00:00:00
abstract::We examined 78 fetuses on d 21 of gestation (G21) and 83 neonates on d 2 after birth (A2), which were first generation offspring of WKY rats mated with normal Wistar rats (F1). In addition, we examined six groups of fetuses on d 19 of gestation (G19): 65 Wistar rats, 111 WKY rats, 85 F1, 100 F1 X F1, 92 F1 X Wistar, a...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199109000-00004
更新日期:1991-09-01 00:00:00
abstract::In Brazil, similar intrauterine growth restriction (IUGR) rates were observed between cities with distinct levels of socioeconomic development, challenging the current knowledge that higher rates of IUGR would necessarily be observed in poorer areas than in wealthier ones. Ribeirão Preto, a city located in the most de...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000156504.29809.26
更新日期:2005-05-01 00:00:00
abstract::Up to 20% of pregnant women smoke and there is indirect evidence that certain tobacco-specific metabolites can cross the placental barrier and are genotoxic to the fetus. The presence of micronuclei results from chromosome damage and reflects the degree of underlying genetic instability. Fetal blood was obtained from ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000228327.63820.41
更新日期:2006-08-01 00:00:00
abstract::A model of patent ductus arteriosus in premature lambs was created to examine the lamb's ability to handle the volume load imposed by a patent ductus arteriosus and to determine the lamb's ability to handle any additional volume load. Fifteen preterm lambs [133 +/- 2 (+/- SD) days gestation, term 145 days], whose duct...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198711000-00012
更新日期:1987-11-01 00:00:00
abstract::In a patient with extrapyramidal movement disorder and extremely low creatinine concentrations in serum and urine, in vivo proton magnetic resonance spectroscopy disclosed a generalized depletion of creatinine in the brain. Oral substitution of arginine, a substrate for creatine synthesis, resulted in an increase of b...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199409000-00023
更新日期:1994-09-01 00:00:00
abstract::Little information is available regarding the time, rhythm, number, and appropriate dosage of human chorionic gonadotropin (HCG) for adequate testing of testicular function in human. The time course of the effect of two, three, or seven HCG injections at intervals of one, five, and two days, respectively, on the plasm...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198006000-00009
更新日期:1980-06-01 00:00:00
abstract::BackgroundAdults born preterm have higher levels of cardiometabolic risk factors than their term-born peers. Studies have suggested that at least those born smallest eat less healthily. We examined the association between early (<34 weeks) and late (34-36 weeks) preterm birth and diet and food preferences in adult age...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2017.301
更新日期:2018-03-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2012.151
更新日期:2013-01-01 00:00:00
abstract::Our goal was to determine the relationship between 4 amniotic fluid (AF) proteomic biomarkers (human neutrophil defensins 2 and 1, calgranulins C and A) characteristic of intra-amniotic inflammation, and funisitis and early-onset sepsis in premature neonates. The mass restricted (MR) score was generated from AF obtain...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.pdr.0000252439.48564.37
更新日期:2007-03-01 00:00:00
abstract::Prematurity is associated with delayed postnatal activation of mitochondrial oxidative phosphorylation and impaired switch from glycolytic to oxidative metabolism. Fatty acids (FA), which represent a major energy substrate in mature muscle cells, are engaged in the postnatal activation of genes of energy metabolism an...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000054687.07095.0B
更新日期:2003-04-01 00:00:00
abstract::Hypoxic-ischemic (HI) injury in the preterm neonate incurs numerous functional deficits, however little is known about the neurochemically-defined brain nuclei that may underpin them. Key candidates are the brainstem catecholamine neurons. Using an immature animal model, the postnatal day (P)-3 (P3) rat pup, we invest...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181659774
更新日期:2008-04-01 00:00:00
abstract:BACKGROUND:Kawasaki disease was well known for coronary artery abnormalities with few reports of incidence of acute kidney injury (AKI). Our aim was to identify the rate of AKI in patients with Kawasaki disease and its associated factors. METHODS:All patients with Kawasaki disease admitted to a medical center from Feb...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2016.81
更新日期:2016-08-01 00:00:00
abstract::A series of in vivo experiments is described in which [1-13C]phytanic acid was given as an oral substrate to a healthy subject and two patients showing an impairment in phytanic acid degradation, one with Refsum's disease and one with chondrodysplasia punctata. After intake of the substrate by the control in a dose of...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199211000-00016
更新日期:1992-11-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199504000-00006
更新日期:1995-04-01 00:00:00
abstract::Diabetes mellitus is characterized by microangiopathy and increased angiogenic response in various organs. Basic fibroblast growth factor (bFGF) as well as vascular endothelial growth factor (VEGF) are both angiogenic and are involved in vascular endothelial cell growth. The purpose of this study was to determine seru...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199812000-00008
更新日期:1998-12-01 00:00:00
abstract::Obese children have a great risk of hypertension and cardiovascular morbidity in adults. The insulin-like growth factor type II (IGF-II) regulates glucose homeostasis, cardiovascular functions, and lipid metabolism. IGF2 gene variants have shown a strong association with weight, body mass index (BMI), and metabolic pr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181d22757
更新日期:2010-04-01 00:00:00
abstract:BACKGROUND:Heterozygous ATP-binding-cassette subfamily A member 3 (ABCA3) mutations are associated with neonatal respiratory complications. In an adult murine model, we investigated whether Abca3 haploinsufficiency is a predisposing factor for lung injury induced by hyperoxia or mechanical ventilation. METHODS:Abca3 h...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.127
更新日期:2013-10-01 00:00:00
abstract::Alveolar hypoxia increases pulmonary artery pressure in lambs and sheep but increases lung lymph flow only in lambs; the reasons for this are unknown. To test the hypothesis that hypoxia-induced pulmonary venous constriction could in part explain the fluid filtration response in young lambs, eight young lambs (13 +/- ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198712000-00013
更新日期:1987-12-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199512000-00021
更新日期:1995-12-01 00:00:00
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journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/00006450-199407001-00021
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:The aim of this study was to examine in detail levels and patterns of physical activity in preschool children and the effects of gender and BMI on this activity. METHODS:Two hundred and fifty-three children aged 5 y participating in the Copenhagen Prospective Studies on Asthma in Childhood wore an accelerom...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.99
更新日期:2013-09-01 00:00:00
abstract::Neutrophils (PMN) from newborn calves generate significantly less superoxide anion (O2-) than do their adult counterparts after stimulation with direct protein kinase C agonists. To better understand this observation, we compared the activity and kinetics of NADPH oxidase in membrane fractions from phorbol 12-myristat...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199010000-00005
更新日期:1990-10-01 00:00:00
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journal_title:Pediatric research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1203/00006450-200101000-00022
更新日期:2001-01-01 00:00:00
abstract::Epithelial hyperplasia is an early feature of the renal and biliary lesions in autosomal recessive polycystic kidney disease (ARPKD). To explore the cellular basis of this hyperplasia we isolated, cultured, and characterized biliary tract epithelium from common bile duct explants of mice with ARPKD (the BPK mouse) and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199506000-00014
更新日期:1995-06-01 00:00:00
abstract::Our aim was to compare white matter (WM) microstructure in preterm infants with and without punctate WM lesions on MRI using tract-based spatial statistics (TBSS) and probabilistic tractography. We studied 23 preterm infants with punctate lesions, median GA at birth 30 (25-35) wk, and 23 GA- and sex-matched preterm co...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3182182836
更新日期:2011-06-01 00:00:00
abstract::Inflammatory phenomena seem to contribute to the occurrence of perinatal cerebral white matter damage and CP. The stimulus that initiates the inflammation remains obscure. One thousand two hundred forty-six infants born before the 28th postmenstrual week had a protocol ultrasound scan of the brain read concordantly by...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181bf5fab
更新日期:2010-01-01 00:00:00
abstract::The effect of alpha-adrenergic blockade by phentolamine on the pulmonary vascular response to dopamine was studied in chronically prepared newborn lambs. Dopamine was administered at doses of 2.7 micrograms . kg-1 . min-1, 27 micrograms . kg-1 . min-1 and 270 micrograms . kg-1 . min-1 with and without alpha-adrenergic...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198306000-00007
更新日期:1983-06-01 00:00:00
abstract::The fetal and maternal concentration of various plasma proteins alters during pregnancy. Cells in the livers of fetal hamsters accumulate serum amyloid A (SAA) and C-reactive protein (CRP) mRNA, major acute phase reactants, when lipopolysaccharide is administered to the fetal circulation. No fetal SAA or CRP mRNA resp...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199111000-00010
更新日期:1991-11-01 00:00:00