Abstract:
:An in situ molecular hybridization system which will detect retrovirus RNA in the cytoplasm of individual virus-infected cells has been developed. The technique was applied to cells infected with simian sarcoma-leukemia virus, where the virus-specific RNA was detected by hybridization to simian sarcoma-leukemia virus 3H-labeled complementary DNA. The system is useful for detecting viral RNA-containing cells in the presence of an excess of virus-negative cells and for determining which type of cell in a heterogenous population is expressing viral RNA.
journal_name
J Viroljournal_title
Journal of virologyauthors
Kaufman SL,Gallo RC,Miller NRdoi
10.1128/JVI.30.2.637-641.1979subject
Has Abstractpub_date
1979-05-01 00:00:00pages
637-41issue
2eissn
0022-538Xissn
1098-5514journal_volume
30pub_type
杂志文章abstract::Bicistronic RNAs containing the 373-nucleotide-long 5' nontranslated region (NTR) of the classical swine fever virus (CSFV) genome as intercistronic spacer were used to show the presence of an internal ribosome entry site (IRES) in the 5' end of the CSFV genome. By coexpression of the poliovirus 2A protease it was dem...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.1.451-457.1997
更新日期:1997-01-01 00:00:00
abstract::Unintegrated linear and closed circular DNAs of B- and N-tropic endogenous BALB/c murine leukemia virus (MuLV) were extracted from newly infected mouse cells and cleaved with EcoRI, XhoI, PvuI, HindIII, SalI, XbaI, KpnI, SmaI, and PstI restriction endonucleases. The DNA fragments were separated by electrophoresis and ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.35.3.812-823.1980
更新日期:1980-09-01 00:00:00
abstract::Structures of somatically acquired murine leukemia virus (MuLV) genomes present in the DNA of a large panel of MuLV-induced C57BL and BALB/c B and non-T/non-B cell lymphomas were compared with those present in MuLV-induced T-cell lymphomas induced in the same low-"spontaneous"-lymphoma-incidence mice. Analyses were pe...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.57.3.1037-1047.1986
更新日期:1986-03-01 00:00:00
abstract::Hemagglutination (HA) by the mammalian reoviruses is mediated by interactions between the viral sigma 1 protein and sialoglycoproteins on the erythrocyte surface. Three serotype 3 (T3) reovirus strains were identified that do not agglutinate either bovine or type O human erythrocytes (HA negative): T3 clone 43 (T3C43)...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.10.5173-5176.1990
更新日期:1990-10-01 00:00:00
abstract::Candidate foot-and-mouth disease (FMD) DNA vaccines designed to produce viral capsids lacking infectious viral nucleic acid were evaluated. Plasmid DNAs containing a portion of the FMDV genome coding for the capsid precursor protein (P1-2A) and wild-type or mutant viral proteinase 3C (plasmids P12X3C or P12X3C-mut, re...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.5.4454-4457.1998
更新日期:1998-05-01 00:00:00
abstract::Hypoviruses Cryphonectria hypovirus 1 (CHV-1)/EP713, CHV-1/Euro7, and CHV-1/EP721, which infect the chestnut blight fungus Cryphonectria parasitica, differ in their degrees of virulence attenuation (hypovirulence), symptom expression, and viral RNA accumulation, even though they share between 90% and 99% amino acid se...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00961-12
更新日期:2012-12-01 00:00:00
abstract::The herpes simplex virus type 1 (HSV-1) genome contains three origins of DNA replication, one copy of oriL and two copies of oriS. Although oriL and oriS are structurally different, they have extensive nucleotide sequence similarity and can substitute for each other to initiate viral DNA replication. A fundamental que...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.5.3580-3587.1997
更新日期:1997-05-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is the causative agent of adult T-cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The HTLV-1 genome encodes the Tax protein that plays essential regulatory roles in HTLV-1 replication and oncogenic transformation of T lymphocytes....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02006-13
更新日期:2013-12-01 00:00:00
abstract::We have previously reported that chimpanzees chronically infected with hepatitis C virus (HCV) could be reinfected, even with the original infecting strain. In this study we tested the hypothesis that this might reflect the presence of minor quasispecies to which there was little or no immunity. To evaluate this hypot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.3.1725-1730.1998
更新日期:1998-03-01 00:00:00
abstract::The large scale of the Ebola virus disease (EVD) outbreak in West Africa in 2013-2016 raised the question whether the host cell interactions of the responsible Ebola virus (EBOV) strain differed from those of other ebolaviruses. We previously reported that the glycoprotein (GP) of the virus circulating in West Africa ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00177-17
更新日期:2017-04-13 00:00:00
abstract::The pandemic H1N1 virus of 2009 (2009 H1N1) continues to cause illness worldwide, primarily in younger age groups. To better understand the pathogenesis of these viruses in mammals, we used a mouse model to evaluate the relative virulence of selected 2009 H1N1 viruses and compared them to a representative human triple...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02742-09
更新日期:2010-05-01 00:00:00
abstract::The simian-human immunodeficiency virus SHIV-HXBc2 contains the envelope glycoproteins of a laboratory-adapted, neutralization-sensitive human immunodeficiency virus type 1 variant, HXBc2. Serial in vivo passage of the nonpathogenic SHIV-HXBc2 generated SHIV KU-1, which causes rapid CD4(+) T-cell depletion and an AIDS...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4208-4218.2001
更新日期:2001-05-01 00:00:00
abstract::Human immunodeficiency virus type 1 infection of mononuclear phagocytes has been implicated in disease manifestations, but postentry viral replication events in these cells have not been well characterized. Productive infection of activated T cells is associated with cell proliferation and accumulation of full-length ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.2.1258-1263.1994
更新日期:1994-02-01 00:00:00
abstract::The expression strategy of parvoviruses of the Densovirus genus has as yet not been reported. Clones were obtained from the densonucleosis virus of Galleria mellonella (GmDNV) that yielded infectious virus upon transfection into LD652 cells. Its genome was found to be the longest (6,039 nucleotides [nt]), with the lar...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.77.19.10357-10365.2003
更新日期:2003-10-01 00:00:00
abstract::The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage site N terminal of ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00356-15
更新日期:2015-08-01 00:00:00
abstract::The hepatitis C virus (HCV) core gene is more conserved at the nucleic acid level than is necessary to preserve the sequence of the core protein, suggesting that it contains information for additional functions. We used a battery of anticore antibodies to test the hypothesis that the core gene directs the synthesis of...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01679-08
更新日期:2009-04-01 00:00:00
abstract:UNLABELLED:The role of NF-κB in influenza A virus (IAV) infection does not reveal a coherent picture, as pro- and also antiviral functions of this transcription factor have been described. To address this issue, we used clustered regularly interspaced short palindromic repeat with Cas9 (CRISPR-Cas9)-mediated genome eng...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00946-16
更新日期:2016-08-12 00:00:00
abstract::Simian virus 40 (SV40)-transformed human cells (LN-SV) were fused with BALB/c peritoneal macrophages (BALB/c X LN-SV) and with C57BL peritoneal macrophages (C57BL X LN-SV) and hybrid clones, all of which had segregated human chromosomes and contained the entire complement of mouse chromosomes, were isolated. All 15 BA...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.18.3.1143-1146.1976
更新日期:1976-06-01 00:00:00
abstract::The genome of bacteriophage phi 6 contains three segments of double-stranded RNA. Procapsid structures whose formation was directed by cDNA copies of the large genomic segment are capable of packaging the three viral message sense RNAs in the presence of ATP. Addition of UTP, CTP, and GTP results in the synthesis of m...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.5.2611-2616.1992
更新日期:1992-05-01 00:00:00
abstract::A total of 51 restriction sites were recognized within the BK virus genome by the combination of 10 different restriction endonucleases. These sites were mapped and oriented relative to one another as well as to the five fragments generated by the digestion of BK virus DNA with HindIII and EcoRI. The result was a comp...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.29.3.915-925.1979
更新日期:1979-03-01 00:00:00
abstract::Human papillomaviruses (HPVs) are small DNA tumor viruses that are the causative agent of warts and are associated with many anogenital cancers. The viral gene encoding the E6 protein has been found to be involved in HPV oncogenesis. E6 is known to inactivate the cellular tumor suppressor, p53. In addition, E6 has bee...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.24.13039-13048.2002
更新日期:2002-12-01 00:00:00
abstract::Ribonucleic acid extracted from influenza virus was labeled at the 3' termini with (3)H and analyzed by polyacrylamide gel electrophoresis. Influenza virus was found to contain a minimum of seven and possibly as many as 10 polynucleotide chains, most of which appear to terminate at the 3' end in uridine. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.8.5.701-707.1971
更新日期:1971-11-01 00:00:00
abstract::The phosphoprotein (P) of the nonsegmented negative-sense RNA viruses is a multimeric modular protein that is essential for RNA transcription and replication. Despite great variability in length and sequence, the architecture of this protein is conserved among the different viral families, with a long N-terminal intri...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01789-19
更新日期:2020-02-28 00:00:00
abstract::Human metapneumovirus (HMPV) has recently been identified as a significant cause of serious respiratory tract disease in humans. In particular, the emerging information on the contribution of HMPV to pediatric respiratory tract disease suggests that it will be important to develop a vaccine against this virus for use ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.23.12877-12887.2004
更新日期:2004-12-01 00:00:00
abstract::Identification of cell surface viral binding proteins is important for understanding viral attachment and internalization. We have fused the pre-S domain of the duck hepatitis B virus (DHBV) large envelope protein to glutathione S-transferase and demonstrated a 170-kDa binding protein (p170) in [35S]methionine-labeled...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.69.11.7106-7112.1995
更新日期:1995-11-01 00:00:00
abstract::Open reading frame P (ORF P), located at the 3' terminus of the 8.5-kb DNA sequence transcribed during latency and almost completely antisense to the gamma(1)34.5 gene, is naturally repressed by infected cell protein 4 (ICP4), the major herpes simplex virus 1 regulatory protein. Earlier studies on cells infected with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.10.7750-7757.1997
更新日期:1997-10-01 00:00:00
abstract:UNLABELLED:The emergence of Middle East respiratory syndrome-coronavirus (MERS-CoV) in the Middle East since 2012 has caused more than 900 human infections with ∼40% mortality to date. Animal models are needed for studying pathogenesis and for development of preventive and therapeutic agents against MERS-CoV infection....
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03427-14
更新日期:2015-04-01 00:00:00
abstract::Measles virus (MV) fusion requires the participation of both the fusion (F) and hemagglutinin (H) glycoproteins. The canine distemper virus fusion protein (CDVF) cannot substitute for the measles virus fusion protein (MVF) in this process. Introduction of restriction enzyme sites into the cDNAs of CDVF and MVF by site...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.68.11.7546-7548.1994
更新日期:1994-11-01 00:00:00
abstract::Human T-cell leukemia virus (HTLV) Tax protein has been implicated in the HTLV oncogenic process, primarily due to its pleiotropic effects on cellular genes involved in growth regulation and cell cycle control. To date, several approaches attempting to correlate Tax activation of the CREB/activating transcription fact...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.6.2655-2662.2000
更新日期:2000-03-01 00:00:00
abstract::Mice and cattle injected with plasmids encoding bovine herpesvirus 1 (BHV-1) glycoproteins developed gene-specific antibody responses capable of neutralizing BHV-1. The ability of animals to respond serologically to DNA injections was in part dependent on the quantity of DNA injected and was also negatively affected b...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.67.9.5664-5667.1993
更新日期:1993-09-01 00:00:00