Abstract：
OBJECTIVE:Molecular chaperone 78 kDa glucose-regulated protein (GRP78) is a residential protein in the endoplasmic reticulum (ER) that is induced by an unfolded-protein response triggered under many kinds of stress against a cell. GRP78 is also known to act as an anti-apoptotic factor by protecting ER-stress-induced cell death. In this study, we examined the significance of GRP78 expression in endometrial cancer. METHODS:Tissue samples obtained from patients with a diagnosis of enodometrial cancer were subjected to immunohistochemistry and RT-PCR to determine protein and mRNA expression levels of GRP78 and estrogen receptor α. We used Western blot and RT-PCR to examine whether estrogen induced GRP78 expression in cancer cell lines. Western blots and MTT assays of GRP78 siRNA transfected Ishikawa and HHUA cells were used to demonstrate whether GRP78 is involved in chemoresistence. RESULTS:GRP78 was highly expressed in well and moderately differentiated endometrial carcinoma. Estrogen induced GRP78 expression, which was correlated with cell viability and resistance to paclitaxel and cisplatin. Western blot analysis indicated that active caspase-3 and the 85-kDa protein poly (ADP-ribose) polymerase (PARP) were increased by incubation with either paclitaxel or cisplatin, suggesting that the apoptotic pathway was involved in cancer-drug-induced cell death. CONCLUSIONS:These results may open up a novel therapeutic strategy for endometrial cancer: namely, the targeting of GRP78 to sensitize the tumor cell to chemotherapy.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Luvsandagva B,Nakamura K,Kitahara Y,Aoki H,Murata T,Ikeda S,Minegishi Tdoi
10.1016/j.ygyno.2012.04.025subject
Has Abstractpub_date
2012-07-01 00:00:00pages
132-9issue
1eissn
0090-8258issn
1095-6859pii
S0090-8258(12)00280-6journal_volume
126pub_type
杂志文章abstract：BACKGROUND:Non-Hodgkin's lymphoma (NHL) may involve the lower female genital tract, most often as a manifestation of systemic disease and rarely as a primarily localisation. CASE:A 73-year-old woman, HIV-negative, presented with a 5-month history of a mass in the left Bartholin's gland. The performed biopsy was report...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2002.6828
更新日期：2002-12-01 00:00:00
abstract：OBJECTIVES:To evaluate recurrence-free survival (RFS) and overall survival (OS) for patients who underwent robotic-assisted laparoscopic hysterectomy (RALH) for uterine malignancies. METHODS:Medical records from 372 patients with uterine malignancies who underwent RALH from 3/06 to 3/09 at two institutions were review...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.11.005
更新日期：2013-02-01 00:00:00
abstract：:A case of microinvasive squamous cell carcinoma of the vulva is reported in which regional lymph node recurrence was noted after excisional therapy. Review of the initial pathology revealed only 0.72 mm of invasion. The results of a literature survey regarding microinvasive vulvar carcinoma are discussed. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(89)90123-6
更新日期：1989-07-01 00:00:00
abstract：INTRODUCTION:Focused ultrasound under real-time MR guidance and control (MRgFUS) can be used for the thermal ablation of tissue. Currently this technique is used clinically for the noninvasive treatment of uterine leiomyomas and is in clinical evaluation for breast cancer, adenomyosis and other indications. MRgFUS is b...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.08.079
更新日期：2008-01-01 00:00:00
abstract：OBJECTIVES:Fixed-dose rate gemcitabine plus docetaxel is active as second-line therapy for metastatic uterine leiomyosarcoma. We sought to determine the activity of this regimen as first-line treatment. METHODS:Eligible women with advanced uterine leiomyosarcoma were treated with gemcitabine 900 mg/m(2) over 90 min, o...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2008.03.010
更新日期：2008-06-01 00:00:00
abstract：OBJECTIVES:The objectives of this study were to assess efficacy and toxicity of the combination of bleomycin, etoposide, and cisplatin (BEP) in this Phase II trial as first-line therapy for ovarian stromal malignancies. METHODS:Patients with incompletely resected Stages II-IV or recurrent cancer underwent surgical deb...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1006/gyno.1998.5304
更新日期：1999-02-01 00:00:00
abstract：OBJECTIVES:To determine prognostic factors for survival in ovarian cancer patients treated with intraperitoneal (IP) chemotherapy using ancillary data from cooperative group clinical trials. METHODS:Data were collected from 428 patients with stage III ovarian cancer who underwent optimal surgical cytoreduction (<1 cm)...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2013.04.001
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:To determine overall survival and progression-free survival among patients with high-risk, stage III and IV endometrial cancer who receive paclitaxel and carboplatin (TC) after complete tumor resection. PATIENTS AND METHODS:Patients with stage III/IV endometrial cancer with less than 2 cm of disease after surg...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.03.019
更新日期：2006-11-01 00:00:00
abstract：:Primary vaginal melanoma is an aggressive and rare gynecological malignancy with < 150 reported cases to date. Historically, patients with this disease have a poor prognosis for all types of treatment. In several studies containing small numbers of patients conservative therapy often has been recommended. Eight patien...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1994.1283
更新日期：1994-11-01 00:00:00
abstract：OBJECTIVE:This study aims to identify favorable preoperative characteristics and examine the impact of secondary cytoreductive surgery on survival for patients with recurrent epithelial ovarian carcinoma. METHODS:Patients who underwent cytoreductive surgery for recurrent epithelial ovarian cancer were identified in ou...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.04.006
更新日期：2007-09-01 00:00:00
abstract：BACKGROUND:Several cytokines have been associated with immune regulation and prognosis in ovarian cancer. CD4+CD25+ Tregs and CD3+CD56+ NK-like T cells are involved in the immune response against the tumor. We have investigated the association of cytokines in the ascites from patients with ovarian cancer with these pop...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.10.018
更新日期：2008-02-01 00:00:00
abstract：OBJECTIVES:In preparation for the launch of a gynecologic oncology survivorship program, this study looked at the informational needs of women with gynecologic cancers. Although studies have touched on some of these needs, no published literature has investigated the comprehensive informational needs of gynecologic onc...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2011.10.030
更新日期：2012-03-01 00:00:00
abstract：OBJECTIVES:The objectives of this study were twofold: to prospectively assess whether expressed intention to undergo prophylactic oophorectomy translated into uptake and to evaluate the psychological impact of the procedure in a sample of unaffected women with a strong family history of breast/ovarian cancer. METHODS:...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2002.6737
更新日期：2002-08-01 00:00:00
abstract：OBJECTIVE:Approximately 20% of patients receiving platinum-based chemotherapy for epithelial ovarian cancer (EOC) are refractory or develop early recurrence. Identifying these patients early could reduce treatment-associated morbidity and allow quicker transfer to more effective therapies. Much attention has focused on...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.05.006
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:Wee1-like kinase (Wee1) is a tyrosine kinase which negatively regulates entry into mitosis at the G2 to M-phase transition and has a role in inhibition of unscheduled DNA replication in S-phase. The present study investigated the clinical role of Wee1 in advanced-stage (FIGO III-IV) ovarian serous carcinoma. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2014.07.102
更新日期：2014-10-01 00:00:00
abstract：OBJECTIVE:To compare surgical morbidity and clinical-pathologic factors for patients with endometrial cancer (EC) undergoing robotic-assisted laparoscopic hysterectomy (RALH) versus total abdominal hysterectomy (TAH) with aortic and/or pelvic lymphadenectomy (LA). METHODS:During the first 14 months of a robotics surgi...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.08.025
更新日期：2008-12-01 00:00:00
abstract：BACKGROUND:The ovarian yolk sac tumor (OYST) is a very rare malignancy arising in young women. Our objective was to determine whether an early decline in serum alpha-fetoprotein (AFP) during chemotherapy has a prognostic impact. METHODS:This retrospective study is based on prospectively recorded OYST cases at Gustave ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2016.07.005
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVE:Debate continues about optimal management of patients with node-positive stage I cervical cancer. Our objective was to determine if patient outcomes are affected by radical hysterectomy in the modern era of adjuvant chemoradiation. METHODS:Cervical cancer patients diagnosed from 2000 to 2008 were identified....
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.03.046
更新日期：2012-07-01 00:00:00
abstract：BACKGROUND:Cutaneous lymphangitis carcinomatosis from cervical carcinoma is a very rare form of tumor metastatization; only anecdotal cases are reported in the literature. Most of the patients with skin relapse experienced metastasis as a single or multiple nodules. CASE:A case of cutaneous lymphangitis carcinomatosis...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.2002.6808
更新日期：2002-10-01 00:00:00
abstract：:The optimal treatment of early-stage ovarian borderline tumors is controversial. Only a few randomized trials evaluating adjuvant treatment for this disease have been published. Between 1970 and 1988 four consecutive randomized trials, including patients with ovarian borderline tumors, were conducted at the Norwegian ...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章,随机对照试验,评审
doi：10.1006/gyno.1993.1279
更新日期：1993-11-01 00:00:00
abstract：:Studies using serological and culture techniques indicate that chlamydial infection is frequently associated with cervical intraepithelial neoplasia (CIN). This relationship was investigated by examining a series of biopsies containing normal epithelium, non-neoplastic condylomatous epithelium, and neoplastic epitheli...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(84)90163-x
更新日期：1984-09-01 00:00:00
abstract：OBJECTIVES:To refine models to predict surgical morbidity and 90-day mortality after primary debulking surgery (PDS) for advanced epithelial ovarian cancer (EOC). METHODS:Women with stage IIIC/IV EOC who underwent PDS with curative intent between 1/2/2003 and 12/30/2011 were included. Patient characteristics, intraope...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2015.10.025
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:Therapy related acute myeloid leukemia (t-AML) is a potential late complication of cytotoxic therapy, and it is of particular concern in the treatment of patients with epithelial ovarian carcinoma (EOC) exposed to multiple courses of chemotherapy during the course of their disease. This study examines the inc...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2011.07.097
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVES:To determine if SAHA, a histone deacetylase inhibitor, decreases ovarian cancer cell viability when combined with paclitaxel in vitro, and to explore molecular alterations of combined paclitaxel+SAHA treatment. METHODS:SKOV3 and Hey ovarian cancer cell lines were treated for 24 h with paclitaxel, then re-tr...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2009.09.039
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To quantify the effect of race/ethnicity on risk of complete and partial molar pregnancy. METHODS:We conducted a cross-sectional study including women who were followed for complete or partial mole and those who had a live singleton birth in a teaching hospital in the northeastern United States between 2000 ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2016.07.117
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:Lymphadenectomy is an integral part of staging and treatment of gynecologic malignancies. We evaluated the feasibility and oncologic value of laparoscopic transperitoneal pelvic and paraaortic lymphadenectomy in correlation to complication rate and body mass index. METHODS:Between August 1994 and September 2...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.07.025
更新日期：2004-10-01 00:00:00
abstract：:To determine the best noninvasive means of evaluating response in patients with advanced ovarian carcinoma, 50 abdominal ultrasound (US) and computed tomography (CT) scans were performed in clinically disease-free ovarian cancer patients. The scans were correlated with the results obtained at a subsequent second- or t...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90348-o
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVE:Mucinous borderline ovarian tumor (mucinous-BOT) and invasive well-differentiated mucinous ovarian cancer (mucinous-OC) are often histopathologically misclassified. The objective of this study was to examine differences in clinico-pathological characteristics and outcomes of these two entities. METHODS:This ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2019.02.003
更新日期：2019-05-01 00:00:00
abstract：:A number of chemical agents have been found to influence the proliferation, morphology, enzymatic activity, and antigen expression of neoplastic cells toward a more differentiated phenotype. We studied the effects of differentiating agents retinoic acid, sodium butyrate, and dibutyryl cyclic AMP on the expression of t...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(91)90356-a
更新日期：1991-09-01 00:00:00
abstract：OBJECTIVES:This trial was conducted to evaluate the safety and efficacy of cisplatin plus gemcitabine in previously treated squamous cell carcinoma of the cervix. SUBJECTS AND METHODS:All women had measurable histologically confirmed squamous cell cervical cancer and a GOG performance status less than or equal to 2. T...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2005.09.009
更新日期：2006-02-01 00:00:00
