当前位置： SCI文献检索 > LIFE SCIENCES期刊下所有文献 > Lack of biological activity of preproendothelin [110-130] in several endothelin assays.

Lack of biological activity of preproendothelin [110-130] in several endothelin assays.

Abstract：

:A 21 amino acid peptide containing the prepropendothelin sequence from amino acids 110 to 130 and two intrachain disulfide bonds was synthesized and tested for biological activity in the following endothelin assays: 1.) a competition binding assay using [125I]ET-1 and dog heart membranes, 2.) three RIA's using 125I-ET-1, -2 and -3 and the respective anti-ET rabbit antisera; and 3.) a contractile activity bioassay using hamster aortic rings. The synthetic peptide which has been referred to as the "endothelin-like" peptide occurs 36 amino acids C-terminal to endothelin in the prepro-protein sequence. It contains only 40% sequence homology to the three endothelin isoforms, but has the same sequence and cyclization pattern of cysteines at positions 1, 3, 11 and 15. Despite the overall similarity in secondary structure to the three isoforms of endothelin and sarafotoxin S6b, preproendothelin [110-130] had no activity in any of the assays when tested at concentrations of 10(-10)M to 10(-5)M.

journal_name

Life Sci

journal_title

Life sciences

authors

Cade C,Lumma WC Jr,Mohan R,Rubanyi GM,Parker-Botelho LH

doi

10.1016/0024-3205(90)90308-e

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

2097-103

issue

23

eissn

0024-3205

issn

1879-0631

pii

0024-3205(90)90308-E

journal_volume

47

pub_type

杂志文章

相关文献

LIFE SCIENCES文献大全
  • Effect of nasal instillation of female urine or vaginal exudate on serum cortisol and prolactin levels in isolated and anesthetized male rhesus monkeys (Macaca mulatta).

    abstract：:Two male adult rhesus monkeys were used and caged individually. The room was temperature-controlled having a light-dark period of 12/12 hours. The animals were rapidly immobilized and immediately anesthetized with ketamine i. m. (10 mg/kg of body weight). They were bled four times at 15, 30, 45 and 60 mins after the k...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(91)90247-9

    authors： Mora OA,García Castiñeiras S,Ezquerra C,Guisado S

    更新日期：1991-01-01 00:00:00

  • MicroRNA let-7g inhibited hypoxia-induced proliferation of PASMCs via G0/G1 cell cycle arrest by targeting c-myc.

    abstract：AIMS:Pulmonary hypertension (PH) is a proliferative disorder characterized by enhanced proliferation and suppressed apoptosis of intrapulmonary vascular smooth muscle cells. Recently, network-based bioinformatics have identified let-7 family, a tumor suppressive microRNA, regulate multiple interacting targets relevant ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2016.11.020

    authors： Zhang WF,Xiong YW,Zhu TT,Xiong AZ,Bao HH,Cheng XS

    更新日期：2017-02-01 00:00:00

  • Captopril attenuates reflex adrenergic response in essential hypertension.

    abstract：:An attenuation of adrenergic activity during the inhibition of endogenous angiotensin II formation was evaluated by determining plasma norepinephrine concentration after a single oral administration of captopril compared to that after nifedipine in essential hypertension. Captopril produced a fall in mean arterial pre...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(91)90415-8

    authors： Mohara O,Masuyama Y

    更新日期：1991-01-01 00:00:00

  • Telmisartan attenuates diabetic nephropathy progression by inhibiting the dimerization of angiotensin type-1 receptor and adiponectin receptor-1.

    abstract：AIMS:The heterodimerization of angiotensin II receptors (AT1R and AT2R) with adiponectin receptor AdipoR1 and AdipoR2 may instigate high glucose (HG)-induced renal tubulointerstitial injury. This study examined the effect of telmisartan on diabetic nephropathy (DN) and its underlying mechanism. MAIN METHODS:Diabetes w...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2019.01.044

    authors： Zha D,Yao T,Bao L,Gao P,Wu X

    更新日期：2019-03-15 00:00:00

  • Increased cold allodynia following intrathecal N-methyl-D-aspartate in rats with a mononeuropathy.

    abstract：:NMDA receptors are involved in the modulation of neuropathic pain behavior and central sensitization. In vivo, this is mostly demonstrated by the use of specific antagonists such as MK801. Because studies evaluating the direct impact of spinal NMDA in neuropathic pain models are lacking, we performed a series of exper...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2004.10.068

    authors： Vissers KC,Hoffmann VL,Adriaensen HF,Heylen RJ,Meert TF

    更新日期：2005-06-10 00:00:00

  • Over expression of bone morphogenetic protein-3b (BMP-3b) using an adenoviral vector promote the osteoblastic differentiation in C2C12 cells and augment the bone formation induced by bone morphogenetic protein-2 (BMP-2) in rats.

    abstract：:BMP-3b is a novel BMP-3-related protein and its biological functions are unknown. In order to investigate the biological actions of BMP-3b, we constructed a BMP-3b-expressing recombinant adenoviral vector (AxCAKBMP-3b). We show that over expression of BMP-3b stimulated the induction of differentiation and the osteoind...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(02)02477-3

    authors： Kaihara S,Bessho K,Okubo Y,Sonobe J,Komatsu Y,Miura M,Miyatake S,Nakao K,Iizuka T

    更新日期：2003-02-28 00:00:00

  • Involvement of reduced acetylcholine release in Delta9-tetrahydrocannabinol-induced impairment of spatial memory in the 8-arm radial maze.

    abstract：:To clarify the mechanism by which Delta9-tetrahydrocannabinol, a major psychoactive component of marijuana, impairs spatial memory in the 8-arm radial maze in rats via the cholinergic system, we used two acetylcholinesterase inhibitors, physostigmine and tetrahydroaminoacridine. Moreover, we examined the effect of Del...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(02)02274-9

    authors： Mishima K,Egashira N,Matsumoto Y,Iwasaki K,Fujiwara M

    更新日期：2002-12-20 00:00:00

  • Enhancement of gastric mucosal integrity by Lactobacillus rhamnosus GG.

    abstract：:The gastric mucosa is frequently exposed to different exogenous and endogenous ulcerative agents. Alcoholism is one of the risk factors for the development of mucosal damage in the stomach. This study aimed to assess if a probiotic strain Lactobacillus rhamnosus GG (LGG) is capable of protecting the gastric mucosa fro...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2007.03.018

    authors： Lam EKY,Tai EKK,Koo MWL,Wong HPS,Wu WKK,Yu L,So WHL,Woo PCY,Cho CH

    更新日期：2007-05-16 00:00:00

  • 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenamide (Z23), an effective compound from the Chinese herb medicine Fissistigma oldhamii (Hemsl.) Merr, suppresses T cell-mediated immunity in vitro and in vivo.

    abstract：:Fissistigma oldhamii (Hemsl.) Merr [F. oldhamii], a traditional Chinese herb medicine, is widely used for treating rheumatoid arthritis (RA) in China. Following bioactivity-guided isolation, a representative immunosuppressive compound with low cytotoxicity, 7'-(3',4'-dihydroxyphenyl)-N-[(4-methoxyphenyl)ethyl]propenam...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2007.10.004

    authors： Hu XD,Zhong XG,Zhang XH,Zhang YN,Zheng ZP,Zhou Y,Tang W,Yang Y,Yang YF,Hu LH,Zuo JP

    更新日期：2007-12-14 00:00:00

  • The role of oxidative stress in developmental and reproductive toxicity of tamoxifen.

    abstract：:The antiestrogen tamoxifen (TAM) is widely used as a drug against breast cancer and is currently being tested as a chemopreventive agent. However, a number of studies showed genotoxic and carcinogenic effects of TAM. These effects are thought to be related to oxygen radical overproduction which occurs during TAM metab...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(01)00969-9

    authors： Pagano G,de Biase A,Deeva IB,Degan P,Doronin YK,Iaccarino M,Oral R,Trieff NM,Warnau M,Korkina LG

    更新日期：2001-03-02 00:00:00

  • Evidence that nitric oxide modulates food intake in mice.

    abstract：:Nitric oxide (NO) may be an intercellular modulator within the central nervous system. L-arginine, which results in NO synthesis, increased food intake in mice while the inhibitor of NO synthesis, L-NG-nitro arginine (L-NO Arg) inhibited food intake in food deprived mice. L-arginine, but not D-arginine, partially reve...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(91)90102-h

    authors： Morley JE,Flood JF

    更新日期：1991-01-01 00:00:00

  • Aging and sex influence the permeability of the blood-brain barrier in the rat.

    abstract：:The aim of the present study was to investigate the existence of aging- and sex-related alterations in the permeability of the blood-brain barrier (BBB) in the rat, by calculating a unidirectional blood-to-brain transfer constant (Ki) for the circulating tracer [14C]-alpha-aminoisobutyric acid. We observed that: a) th...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(90)90157-m

    authors： Saija A,Princi P,D'Amico N,De Pasquale R,Costa G

    更新日期：1990-01-01 00:00:00

  • Effects of tacrine (THA) on spatial reference memory and cholinergic enzymes in specific rat brain regions.

    abstract：:Cognitive function of rats treated with saline (control), THA (8 mg/kg, i.p.), scopolamine (5 mg/kg, i.p.), or a combination of THA (8 mg/kg) and scopolamine (5 mg/kg) was tested in the Morris water maze. The latency to find the platform in the water maze was used to evaluate performance. THA did not significantly alt...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(95)02254-6

    authors： Jackson JJ,Soliman MR

    更新日期：1996-01-01 00:00:00

  • Vitamin E, membrane fluidity, and blood pressure in hypertensive and normotensive rats.

    abstract：:Vitamin E treatment was found to lower blood pressure, and increase membrane fluidity in rats. The objectives of this study were to investigate the effects of the antioxidant, vitamin E, on the blood pressure and erythrocyte membrane fluidity in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Membrane fl...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(00)00775-x

    authors： Pezeshk A,Derick Dalhouse A

    更新日期：2000-09-01 00:00:00

  • The benefits of coffee on skeletal muscle.

    abstract：:Coffee is consumed worldwide with greater than a billion cups of coffee ingested every day. Epidemiological studies have revealed an association of coffee consumption with reduced incidence of a variety of chronic diseases as well as all-cause mortality. Current research has primarily focused on the effects of coffee ...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2015.11.005

    authors： Dirks-Naylor AJ

    更新日期：2015-12-15 00:00:00

  • Problems in the measurement of zinc using heparin as an anticoagulant.

    abstract：:Although several reports imply that anticoagulants and preservatives contain zinc, the quantity of zinc in heparin, if any, has not been documented. Zinc concentration was determined by flame atomic absorption spectroscopy in varying dilutions of multiple commercially obtained samples of purified sodium heparin N = 15...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(83)90348-x

    authors： Gervin CA,Gervin AS,Nichols W,Corrigan JJ Jr

    更新日期：1983-12-26 00:00:00

  • Luteolin sensitizes human 786-O renal cell carcinoma cells to TRAIL-induced apoptosis.

    abstract：AIMS:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been considered to be one of the most promising candidates in research on treatments for cancer, including renal cell carcinoma (RCC). However, many cells are resistant to TRAIL-induced apoptosis which limits the potential application of TRAIL in ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2014.02.002

    authors： Ou YC,Li JR,Kuan YH,Raung SL,Wang CC,Hung YY,Pan PH,Lu HC,Chen CJ

    更新日期：2014-04-01 00:00:00

  • Ruthenium red-sensitive cation channels, but not calcitonin gene-related peptide or substance P-mediated mechanisms, protect duodenal villi against acid-induced damage.

    abstract：:Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the protective mesenteric hyperemia after intraduodenal acidification. Mechanisms associated the sensory neuropeptides, e.g. calcitonin gene-related peptide (CGRP), substance P, and ruthenium red-sensitive cation channels contribute to acid-induc...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(02)02106-9

    authors： Leung FW,Iwata F,Kao J,Seno K,Itoh M,Leung JW

    更新日期：2002-10-18 00:00:00

  • Aggravation of DMCM-induced seizure by nitric oxide synthase inhibitors in mice.

    abstract：:The present study investigated the effects of nitric oxide synthase (NOS) inhibitors on the seizure threshold of DMCM in mice. The seizure threshold of DMCM was evaluated using an intravenous infusion technique. The threshold of DMCM was significantly decreased by pretreatment with N-nitro-L-arginine (NOARG; 8 mg/kg) ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(97)00226-9

    authors： Tsuda M,Suzuki T,Misawa M

    更新日期：1997-01-01 00:00:00

  • Changes in high affinity sodium independent gamma-aminobutyric acid binding in cerebral cortex and hippocampus of the rat following electroshock.

    abstract：:Electroshock induced seizures in the rat enhanced high affinity specific Na+ independent binding of 3H-gamma aminobutyric acid (GABA) to frozen, Triton X-100 treated cerebral synaptic membranes 30 minutes after exposures to electroshock, although no change in 3H GABA binding was observed in similarly treated preparati...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(82)90756-1

    authors： Ross SM,Craig CR

    更新日期：1982-11-29 00:00:00

  • Sumoylation enhances the activity of the TGF-β/SMAD and HIF-1 signaling pathways in keloids.

    abstract：:Excessive fibrosis and extracellular matrix deposition resulting from upregulation of target genes expression mediated by transforming growth factor-beta (TGF-β)/SMAD and hypoxia inducible factor-1 (HIF-1) signaling pathways are the main mechanisms that drive keloid formation. Sumoylation is a protein posttranslationa...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.117859

    authors： Lin X,Wang Y,Jiang Y,Xu M,Pang Q,Sun J,Yu Y,Shen Z,Lei R,Xu J

    更新日期：2020-08-15 00:00:00

  • Effects of oxymorphazone in frogs: long lasting antinociception in vivo, and apparently irreversible binding in vitro.

    abstract：:Oxymorphazone (at doses of 50-200 mg/kg) was found to be a relatively weak antinociceptive drug in intact frog (Rana esculenta) when acetic acid was used as pain stimulus. Frogs remained analgesic for at least 48 hrs following oxymorphazone (200 mg/kg) administration. The ligand increased the latency of wiping reflex ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(89)90302-0

    authors： Benyhe S,Hoffmann G,Varga E,Hosztafi S,Toth G,Borsodi A,Wollemann M

    更新日期：1989-01-01 00:00:00

  • Weak binding of 10-hydroxymetabolites of nortriptyline to rat brain muscarinic acetylcholine receptors.

    abstract：:The relative affinities of nortriptyline (NT) and its 10-hydroxymetabolites to rat brain muscarinic acetylcholine receptors have been determined by competition with 3H-quinuclidinyl benzilate binding. It is shown that the major NT metabolite, E-10-OH-NT, has only 1/18 the affinity of NT for the muscarinic receptor. Si...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(84)90395-3

    authors： Wägner A,Ekqvist B,Bertilsson L,Sjöqvist F

    更新日期：1984-09-24 00:00:00

  • Somatostatin antagonist analogue stimulates growth in rats.

    abstract：:The somatostatin antagonist analogue cyclo Ahep-Phe-D-Trp-Lys-Thr(Bzl) stimulates growth in young female rats, while somatostatin itself has only equivocal effects on inhibiting growth. ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(85)90621-6

    authors： Spencer GS,Hallett KG

    更新日期：1985-07-08 00:00:00

  • The effects of calorie-matched high-fat diet consumption on spontaneous physical activity and development of obesity.

    abstract：AIMS:To characterize the effects of a calorie matched high-fat diet (HFD) on spontaneous physical activity (SPA), body weight, inflammatory status and expression of genes related to energy homeostasis in hypothalamus of mice. MAIN METHODS:C57Bl/6 mice (n=5 per group) were fed a control diet (16.5% calories from fat) -...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2017.04.017

    authors： Moretto TL,Benfato ID,de Carvalho FP,Barthichoto M,Le Sueur-Maluf L,de Oliveira CAM

    更新日期：2017-06-15 00:00:00

  • Drug-induced antinuclear antibodies in the guinea pig.

    abstract：:Antinuclear antibodies (ANA) development was studied in male guinea pigs in response to chronic treatment with procainamide, hydralazine, acetanilide or caffeine. Acetanilide and caffeine have not previously been associated with ANA induction. Fifty-one weanling Hartley guinea pigs were divided into five groups which ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(82)90653-1

    authors： Robinson CJ,Balazs T

    更新日期：1982-01-11 00:00:00

  • Co-administration of dextromethorphan during pregnancy and throughout lactation significantly decreases the adverse effects associated with chronic morphine administration in rat offspring.

    abstract：:In this study, we have focused our investigation of the facts whether co-administration of a NMDA antagonist dextromethorphan (DM) with morphine during pregnancy and throughout lactation could prevent the adverse effects associated with chronic morphine administration in rat offspring. Adult female Sprague-Dawley rats...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(01)01316-9

    authors： Tao PL,Yeh GC,Su CH,Wu YH

    更新日期：2001-10-05 00:00:00

  • Potential of resveratrol in the treatment of heart failure.

    abstract：:The concept of food has expanded beyond its traditional role of survival and hunger satisfaction, to include a role in the prevention and treatment of disease. Polyphenols are classes of compounds that are synthesized by plants to serve a wide variety of functions including growth pollination and defense. These compou...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2013.12.011

    authors： Raj P,Louis XL,Thandapilly SJ,Movahed A,Zieroth S,Netticadan T

    更新日期：2014-01-30 00:00:00

  • Excretion of urinary epidermal growth factor in non-insulin dependent diabetes mellitus.

    abstract：:Morning urine samples were assayed for human EGF (hEGF) in 137 non-insulin dependent diabetic patients with normal serum creatinine and beta 2-microglobulin levels. Serving as controls, 80 age- and sex-matched healthy subjects were also examined. A significant positive correlation between hEGF excretion and the level ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(93)90100-h

    authors： Kawaguchi M,Kamiya Y,Ito J,Fujii T,Hayakawa F,Sakuma N,Fujinami T

    更新日期：1993-01-01 00:00:00

  • Chemerin influences endothelin- and serotonin-induced pulmonary artery vasoconstriction in rats.

    abstract：AIMS:Chemerin has been recently identified as a vasoactive adipokine implicated in blood pressure regulation. In this context, we evaluated whether chemerin could influence pulmonary vasoreactive response. MATERIALS AND METHODS:Vascular reactivity to chemerin and to phenylephrine, serotonin and endothelin-1 after chem...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2019.116580

    authors： Hanthazi A,Jespers P,Vegh G,Degroot GN,Springael JY,Lybaert P,Dewachter L,Mc Entee K

    更新日期：2019-08-15 00:00:00