Abstract:
:Knowledge about the magnitude of individual polymorphism is a critical part in understanding the complexity of comprehensive mismatching. HLA-B*44:09 differs from the highly frequent HLA-B*44:02 allele by amino acid exchanges at residues 77, 80, 81, 82 and 83. We aimed to identify the magnitude of these mismatches on the features of HLA-B*44:09 bound peptides since residues 77, 80 and 81 comprise part of the F pocket which determines sequence specificity at the pΩ position of the peptide. Using soluble HLA technology we determined >200 individual (nonduplicate) self-peptides from HLA-B*44:09 and compared their features with that of the published peptide features of HLA-B*44:02. Both alleles illustrate an anchor motif of E at p2. In contrast to the C-terminal peptide binding motif of B*44:02 (W, F, Y or L), B*44:09-derived peptides are restricted predominantly to L or F. The source of peptides for both alleles is identical (LCL 721.221 cells) allowing us to identify 23 shared peptides. The majority of these peptides however contained the restricted B*44:09 anchor motif of F or L at the pΩ position. Molecular modelling based on the B*44:02 structure highlights that the differences of the C-terminal peptide anchor between both alleles can be explained primarily by the B*44:02(81Ala) > B*44:09(81Leu) polymorphism which restricts the size of the amino acid that can be accommodated in the F pocket of B*44:09. These results highlight that every amino acid substitution has an impact of certain magnitude on the alleles function and demonstrate how surrounding residues orchestrate peptide specificity.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Huyton T,Schumacher H,Blasczyk R,Bade-Doeding Cdoi
10.1007/s00251-012-0625-1subject
Has Abstractpub_date
2012-09-01 00:00:00pages
663-8issue
9eissn
0093-7711issn
1432-1211journal_volume
64pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::The generation and maintenance of allelic polymorphism in genes of the major histocompatibility complex (MHC) is a central issue in evolutionary genetics. Recently, the focus has changed from ex situ to in situ populations to understand the mechanisms that determine adaptive MHC polymorphism under natural selection. B...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-007-0205-y
更新日期:2007-05-01 00:00:00
abstract::Mammalian interleukin-4 (IL-4) and IL-13 are T helper type 2 (Th2) cytokines with pleiotropic functions in immunity. They signal through receptors containing IL-4Rα and IL-2Rγ or IL-13Rα1. In addition, a decoy receptor, IL-13Rα2, is known to exist and modulates the function of IL-13. The existence of fish orthologues ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0508-2
更新日期:2011-04-01 00:00:00
abstract::The A15, L6, and S5.7(TAPA1) proteins are members of the transmembrane 4 superfamily (TM4SF). The A15 is expressed in immature human T cells and in the human brain. The MXS1(CCG-B7) gene which codes for A15 contains triplet nucleotide repeats which have been associated with neuropsychiatric diseases such as Huntington...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00189229
更新日期:1994-01-01 00:00:00
abstract::The anti-HLA-DQ3 monoclonal antibodies (mAb) KS13, SO1, SO2, SO3, SO4, and SO5 recognize spatially close but distinct antigenic determinants, since they crossinhibit each other in their binding to HLA-DQ3 antigens, but do not share idiotopes recognized in their antigen combining site by syngeneic and anti-id antisera ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00178583
更新日期:1995-01-01 00:00:00
abstract::Probes for loci situated near one end of the proximal (Tcp-1) and distal (Qa-2, 3) inversions of the mouse t complex have been hybridized to chromosomes of mice with and without t complexes and with morphologically distinguishable chromosome 17s. Both the probe for Tcp-1 and that for Qa-2, 3 hybridized to clearly diff...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00395134
更新日期:1988-01-01 00:00:00
abstract::Class II genes of the human major histocompatibility complex (MHC) are polymorphic. Allelic variation of the coding region of these genes is involved in the antigen presentation and is associated with susceptibility to certain autoimmune diseases. The DR region is unique among human class II regions in that multiple D...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216386
更新日期:1993-01-01 00:00:00
abstract::The variability of immune responses modulated by human leukocyte antigen (HLA) genes and secreted cytokines is a significant factor in the development of a protective effect of measles vaccine. We studied the association between type 1 helper T cells (Th1)- and Th2-like cytokine immune responses and HLA class I allele...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-005-0061-6
更新日期:2005-12-01 00:00:00
abstract::The membrane immunoglobulin M (mIgM) of a B lymphocyte serves as a receptor for its cognate antigen. Our aim is to elucidate the structure and function of this membrane-bound receptor. The first step is to determine the requirements for proper membrane placement of IgM. We have used mIgM-positive B lymphocyte tumors f...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00364758
更新日期:1983-01-01 00:00:00
abstract::Earlier studies suggest that end-stage renal disease (ESRD) is associated with inflammatory state and have become a major cause of morbidity and mortality worldwide. This study speculated the role of interleukins (IL)-2, -4, and -6 cytokines gene polymorphism with risk of susceptibility to ESRD. Polymorphism in IL-2 (...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0182-6
更新日期:2007-02-01 00:00:00
abstract::Footpad swelling response was used to measure the alloantigenicity of epidermal cells (ECs) in delayed-type hypersensitivity (DTH). Strong footpad swelling was observed 3 h after the challenge, and it continued for 48 h after the challenge. Genetical incompatibility between the recipients and the ECs was required to i...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372666
更新日期:1986-01-01 00:00:00
abstract::In order to further our understanding of major histocompatibility complex (MHC) class I gene organization, we began a comparative analysis of the large scale organization of the class I region in diverse haplotypes. For these studies, the MHC in healthy Japanese donors who have the predominant MHC haplotypes and/or HL...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050252
更新日期:1997-01-01 00:00:00
abstract::The incidence of arthritis and the antibody response to mouse and to rat type II collagen after immunization with native rat type II collagen was studied in different mouse strains, including wild mouse-derived strains belonging to the H-2p/H-2q family. High serum levels of antibodies to mouse and rat type II collagen...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00373114
更新日期:1986-01-01 00:00:00
abstract::Cichlid fishes of the East African Rift Valley lakes constitute an important model of adaptive radiation. Explosive speciation in the Great Lakes, in some cases as recently as 12 400 years ago, generated large species flocks that have been the focus of evolutionary studies for some time. The studies have, however, bee...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050243
更新日期:1997-01-01 00:00:00
abstract::Natural-killer group 2 member D (NKG2D) is an activating receptor that plays an important role in the immune response mediated by NK cells, γδ(+) T cells, and CD8(+) T cells. In humans, MHC class I chain-related genes and UL-16 binding protein (ULBP)/retinoic acid early transcript 1 (REAT1) gene family encode ligands ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0531-y
更新日期:2011-08-01 00:00:00
abstract::We have used seven HLA-D homozygous typing cells (HTC) in a comparative study of the DRw8 antigen complex in three racial groups. Three distinct HLA-D specificities were recognized, each associated with HLA-DRw8. Four of the HTC defined a DRw8-associated HLA-D specificity designated 8.1, one defined a specificity desi...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372458
更新日期:1983-01-01 00:00:00
abstract::The human leukocyte receptor complex (LRC) at Chromosome 19q13.4 encodes Ig superfamily proteins which regulate the function of various hematopoietic cell types. We investigated characteristics of the Ig-like transcript (ILT)/leukocyte Ig-like receptor (LIR) group of LRC genes in comparison with the other major LRC lo...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510100332
更新日期:2001-05-01 00:00:00
abstract::Comparison of the rate of synonymous and nonsynonymous nucleotide substitutions suggests that certain regions of the functional H-2 genes, which are part of the mouse major histocompatibility complex (Mhc), are under strong positive selection pressure. Thus far, however, little evidence has been provided for the exist...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00205819
更新日期:1991-01-01 00:00:00
abstract::Two out of ten Rg-specific antisera tested contain a third antibody specific for the beta chain of C4. Analysis of the beta chains of 66 unrelated individuals by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the epitope detected is located exclusively on the light (L) beta chain. A strong, bu...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF02425274
更新日期:1989-01-01 00:00:00
abstract::Although major histocompatibility complex (MHC) class I molecules are, as a rule, highly polymorphic in mammalian species, those of the New World primate Saguinus oedipus (cotton-top tamarin) exhibit limited polymorphism. We have cloned and sequenced twelve MHC class I cDNAs from this species. Since cloned cotton-top ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00210819
更新日期:1991-01-01 00:00:00
abstract::The polymorphism of major histocompatibility complex class I chain-related gene B (MICB) and variations in MICB alleles in a variety of populations have been characterized using several genotyping approaches. In the present study, a novel polymerase chain reaction sequence-based typing (PCR-SBT) method was established...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-013-0699-4
更新日期:2013-07-01 00:00:00
abstract::Assembly of HLA class I molecules was studied using pulse-chase labeling of B-lymphoblastoid cell lines with 35S-methionine, immunoprecipitation with antibodies detecting free or beta 2-microglobulin-associated heavy chain and isoelectric focusing. Marked differences between the products of different class I alleles w...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216831
更新日期:1993-01-01 00:00:00
abstract::Avian influenza is a serious threat to the poultry industry and, as the potential source of a human pandemic virus, to public health. Different Mx alleles have been reported to confer resistance or susceptibility to influenza virus replication, and so knowledge of their frequencies is important when considering the po...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-007-0235-5
更新日期:2007-08-01 00:00:00
abstract::We studied the relationship between human leukocyte antigen (HLA) class I alleles and cervical cancer among Indian women. Seventy-five cervical cancer cases were compared with 175 noncancer controls. Cervical biopsy tissue specimen from cancer cases and cervical swab specimen from controls were collected for HPV detec...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-014-0805-2
更新日期:2014-12-01 00:00:00
abstract::Phenotypic variability is often observed in cynomolgus monkeys on preclinical studies and may, in part, be driven by genetic variability. However, the role of monkey genetic variation remains largely unexplored in the context of drug response. This study evaluated genetic variation in cynomolgus monkey FcγR3A and TAP1...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-017-0970-1
更新日期:2017-04-01 00:00:00
abstract::The major histocompatibility complex of the rat (RT1) encodes the class II molecules involved with antigen presentation and cell to cell communication. The organization of these class II genes has been studied by Southern blot hybridization using genomic DNA from inbred and recombinant rat strains digested with variou...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00346516
更新日期:1987-01-01 00:00:00
abstract::The major histocompatibility complex (MHC) of the domestic cat has been poorly characterized to date, primarily because of numerous difficulties in the preparation of allotypic sera. We present here a comparative analysis of class I and class II genes in domestic cat populations using molecular probes of the MHC from ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00364427
更新日期:1988-01-01 00:00:00
abstract::The interaction between 9-mer peptides and HLA-B51 molecules was investigated by quantitative peptide binding assay using RMA-S cells expressing human beta2-microglobulin and HLA-B51 molecules. Of 147 chemically synthesized 9-mer peptides possessing two anchor residues corresponding to the motif of HLA-B*5101 binding ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF02440994
更新日期:1996-01-01 00:00:00
abstract::A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. Nucleotide sequence analysis of the two intronless genes, HSP70-1 and HSP70-2, has shown that they encode an identical protein pro...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00187095
更新日期:1990-01-01 00:00:00
abstract::Recent genome-wide association studies (GWAs) have identified several new genetic risk factors for asthma; however, their influence on disease behavior and treatment response is still unclear. The aim of our study was the association analysis of the most significant single nucleotide polymorphisms (SNPs) recently repo...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-013-0755-0
更新日期:2014-03-01 00:00:00
abstract::The rat major histocompatibility complex loci RT1-B and RT1-D are equivalent to the human leucocyte antigens HLA-DQ and HLA-DR respectively. Here we describe the complementary DNA (cDNA) sequence encoding the alpha and beta chains of both the RT1-B and RT1-D locus genes of the rat RT1u haplotype. We have found entire ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0584-7
更新日期:2003-08-01 00:00:00