Abstract:
OBJECTIVE:The aim of this study was to investigate the expression level of micro-ribonucleic acid-1207-5p (miR-1207-5p) in steroid-induced necrosis of femoral head (SNFH) and its correlation with SNFH. Meanwhile, we also aimed to analyze the relationship between miR-1207-5p expression and vascular endothelial growth factor (VEGF) in the femoral head. PATIENTS AND METHODS:From May 2016 to December 2017, 60 patients aged (55.4±8.7) were selected in our hospital. All patients were diagnosed and confirmed as SNFH. Total RNA was extracted from the necrotic femoral head tissues and peripheral blood. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect the expression level of miR-1207-5p in tissues. At the same time, immunohistochemistry and Western blotting were adopted to detect VEGF expression in the bone tissue of patients with high or low expression of miR-1207-5p. 7 patients with femoral neck fracture aged (45.6±4.51) were enrolled in the control group. In the animal experiment, the rat SNFH model was established by intraperitoneal injection of lipopolysaccharide and methylprednisolone. Subsequently, the expression levels of miR-1207-5p and VEGF in necrotic femoral tissues were detected. Meanwhile, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was applied to detect cell apoptosis in bone lacunae of miR-1207-5p high expression group and miR-1207-5p low expression group, respectively. RESULTS:The expression level of miR-1207-5p in the necrotic bone tissue of the SNFH group was significantly higher than that of the control group. The expression level of miR-1207-5p was inversely proportional to Harris Hip score (p<0.05). A higher expression of miR-1207-5p indicated a lower expression level of VEGF (p<0.05). The animal experimental results revealed that miR-1207-5p expression in the necrotic femoral head tissue of SNFH group was significantly higher than that of the control group. Furthermore, the number of apoptotic cells in bone lacunae was remarkably higher in miR-1207-5p high expression group (p<0.05). CONCLUSIONS:MiR-1207-5p is significantly up-regulated in necrotic femoral head tissue and serum of SNFH patients. Meanwhile, its expression level is inversely proportional to Harris Hip score of patients. The possible underlying mechanism may be related to the inhibitory effect of miR-1207-5p on VEGF.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Chao PC,Cui MY,Li XA,Jiang Y,Lin BC,Li ZBdoi
10.26355/eurrev_201904_17541subject
Has Abstractpub_date
2019-04-01 00:00:00pages
2710-2718issue
7eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to investigate the expression level of microRNA-21-5p and its target gene PLAG1 in the serum of patients with spinal cord injury (SCI), as well as the role and mechanism of microRNA-21-5p in lipopolysaccharide-induced primary microglial inflammation. PATIENTS AND METHODS:The levels ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21480
更新日期:2020-06-01 00:00:00
abstract:UNLABELLED:BACKGROUND, OBJECTIVES: Pancreatic cancer ranks fourth for cancer mortality for men and women in the United States. This is a particularly devastating cancer since the case-fatality proportion approaches 90% within 12 months following diagnosis. Therefore, understanding the etiology and identifying the risk ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2012-02-01 00:00:00
abstract:STUDY OBJECTIVES:In this study we evaluated the role of three currently available therapeutic regimens in the treatment of early stages of idiopathic pulmonary fibrosis (IPF). PATIENTS:The study population consisted of 57 consecutive suspected individuals with IPF. Patients with interstitial pneumonias other than IPF ...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2008-03-01 00:00:00
abstract:OBJECTIVE:Dysregulation of numerous oncogenes and their downstream signaling pathways, among others in the signaling transduction molecule p-CREB-1 (p-cAMP responsive element binding protein-1), is an essential feature of different types of cancer. To investigate whether p-CREB-1 is also pivotal in tumorigenesis and me...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202011_23806
更新日期:2020-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the expression of microRNA (miR)-551b in patients with low and high grade cervical intraepithelial neoplasia (CIN) and to find an association with high-risk Human Papillomavirus (HR-HPV) infection-related prognostic biomarkers. PATIENTS AND METHODS:The expression level of miR-551b was determined ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201807_15496
更新日期:2018-07-01 00:00:00
abstract::Urinary tract infections are often associated with urinary anomalies. An appropriate pharmacologic treatment may prevent, or may at least limit, any kidney damage due to pyelonephritis. The antibiotic prophylaxis plays a role as significant as early surgical therapy, taking into consideration also the present limitati...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract:INTRODUCTION:The hysto-morfological composition of the ascending aorta wall gives to the vessel its characteristic elasticity/distensibility, which is deteriorated due to both physiological (age) and pathological events (hypertension, diabetes, dyslipidemia). This contributes to reduce the wall elasticity and to occurr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:To investigate the impact of miR-124Aa on the proliferation, invasion and cytokine excretion of rheumatoid arthritis synovial fibroblasts (RASFs) in patients with rheumatoid arthritis (RA). PATIENTS AND METHODS:RASFs were separated for in-vitro culture, and transfected using lipidosome that connected with ch...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201807_15515
更新日期:2018-07-01 00:00:00
abstract::Ectopic parathyroid adenomas represent a diagnostic challenge, since they are quite rare in clinical practice. We present a case of a 61 years old man with symptomatic hypercalcemia due to an ectopic parathyroid adenoma in the upper-anterior mediastinum that was not localized by the scintigraphy. Ultrasonography ident...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the role of HOTAIR in inflammatory response after acute myocardium infarction (AMI) and to investigate its underlying mechanism. MATERIALS AND METHODS:The AMI model was first constructed in rats, and heart tissues were harvested. Expression levels of HOTAIR and receptor o...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201811_16282
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:Nasopharyngeal carcinoma (NPC) is a malignancy and is prone to distant metastasis and radioresistance. Long non-coding RNAs (lncRNAs) play vital roles in human cancers. The purpose of this study was to explore the role and the action mechanism of intergenic lncRNA (LINC00114) in NPC. MATERIALS AND METHODS:Th...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20517
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:Readmissions to hospital after discharge are considered adverse, serious and costly outcomes. In the last years, two new scores have been proposed to identify patients at high risk of hospital readmission, the HOSPITAL and the Elders Risk Assessment (ERA) indexes. The aim of this study was to evaluate these t...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To analyze the effect of serum levels of high molecular weight adiponectin (HMWA) on the occurrence of eclampsia during subsequent pregnancy in patients with primary pregnancy-induced hypertension (PIH). PATIENTS AND METHODS:Thirty patients with primary PIH (observation group) and sixty patients without prim...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:To explore whether microRNA-138 could regulate the incidence and progression of laryngeal carcinoma through modulating proliferation and apoptosis of laryngeal carcinoma cells via MAPK6. PATIENTS AND METHODS:MicroRNA-138 expression in laryngeal carcinoma tissues and paracancerous tissues were detected by qRT...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15820
更新日期:2018-09-01 00:00:00
abstract:OBJECTIVE:Platelet rich plasma (PRP) is a haemoderivative used in clinical practice for the treatment of hard-to-heal wounds. Platelet (PLT) activation is a key factor in the wound healing process leading to the production of extracellular vesicles. We obtained PRP and PRP-derived microvesicles (PLT-MVs) from healthy d...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202009_23055
更新日期:2020-09-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the effects of oxaliplatin on intestinal floras, inflammation level, apoptosis-related gene expressions and oxidative stress in rats with colorectal cancer. MATERIALS AND METHODS:A total of 30 adult Sprague-Dawley (SD) rats were selected as research objects and were d...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202010_23407
更新日期:2020-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202004_20851
更新日期:2020-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect and mechanism of bone marrow stem cells (BMSCs) modified with CXCR-4 gene combined with stromal derived factor-1α (SDF-1α) in the treatment of acute spinal cord injury (SCI) in rats. MATERIALS AND METHODS:CXCR-4 gene was transfected by a virus. Spinal cord injury rats were randomly div...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-06-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201907_18317
更新日期:2019-07-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract:OBJECTIVE:To explore possible mechanism of ERBB2 gene expression silencing mediating mitogen-activated protein kinase 1/mitogen-activated protein kinase 3 (MAPK1/MAPK3) signaling pathway on proliferation, migration, and invasion of ovarian cancer cells. PATIENTS AND METHODS:A total of 240 cancer specimens were collect...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21309
更新日期:2020-05-01 00:00:00
abstract:INTRODUCTION:In the latest years the use of lung ultrasound is increasing in the evaluation of pleural effusions, because it makes follow-up easier and drainage more efficient by providing guidance on the most appropriate sampling site. However, no standardized approach for ultrasound-guided thoracenthesis is actually ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVES:One important limitation in cell therapy protocols, and regenerative medicine (an innovative and promising strategy for different pathologies treatment), is the lack of knowledge about cells engraftment, proliferation and differentiation. In order to allow an efficient and successful cell transplant, it is n...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the regulatory role of lncRNA 00152 and JAK2/STAT3 pathway in the pathogenesis of hepatocellular carcinoma (HCC), and to investigate the possible underlying mechanism. PATIENTS AND METHODS:Expression levels of lncRNA 00152 in HCC tissues, matched para-cancerous tissues an...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201902_16991
更新日期:2019-02-01 00:00:00
abstract:OBJECTIVE:To discuss the clinical effects of treating secondary asthma attacks of children Mycoplasma pneumoniae with combined therapy of montelukast and azithromycin. PATIENTS AND METHODS:96 children patients diagnosed with secondary asthma attacks of Mycoplasma pneumonia were enrolled in this study. They were random...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-12-01 00:00:00
abstract::Monitoring oxidative stress in humans is achieved by assaying products of oxidative damage or by investigating the potential of an organism, tissue or body fluids to withstand further oxidation. Unfortunately, there is little consensus concerning the selection of parameters of oxidative stress or antioxidant state to ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVES:Aggressive cell growth and chemoresistance are notorious obstacles in neuroblastoma therapy. Accumulating evidence suggests that survivin is preferentially expressed in cancer cells and plays a crucial role in cell division and apoptosis dysfunction. Thus, in the present study, we investigated whether silenc...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00