Abstract：
OBJECTIVE:Platelet rich plasma (PRP) is a haemoderivative used in clinical practice for the treatment of hard-to-heal wounds. Platelet (PLT) activation is a key factor in the wound healing process leading to the production of extracellular vesicles. We obtained PRP and PRP-derived microvesicles (PLT-MVs) from healthy donors and compared their pro-healing efficacy in an in vitro wound model using human keratinocytes. MATERIALS AND METHODS:We evaluated PLT-MVs' direct effect on an in vitro model of wound healing. PRP, PRP activated using calcimycin, and PLT-MVs separated by high speed centrifugation were added to scratched keratinocyte monolayers. Fluorescein diacetate was used in flow cytometry to distinguish PLTs and PLT-MVs from debris, and then, PLT-MVs were quantified on the basis of relative dimensions (Forward Scatter signals). RESULTS:Wound areas were measured at time 0 and after 24 hours and they were healed by 24.80 ± 4.28% in control conditions, while PRP, activated PRP, and PLT-MVs increased closure of 62.94 ± 0.96%, 52.69 ± 17.20% and 52.76 ± 9.44%, respectively. CONCLUSIONS:Our results indicate that PRP pro-healing effects were fully replicable by PLT-MVs, suggesting a key role of microvesicles in the healing process and a possible clinical use as an alternative to PRP.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Lovisolo F,Carton F,Gino S,Migliario M,Renò Fdoi
10.26355/eurrev_202009_23055subject
Has Abstractpub_date
2020-09-01 00:00:00pages
9658-9664issue
18eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract：AIM:To investigate the impacts of infectious complications on mortality and morbidity; and to identify the other potential factors effective in mortality in peritoneal dialysis (PD) patients. PATIENTS AND METHODS:We included patients who initiated therapy between 2001-2011. Patients were divided into two groups regard...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:Dynamic pupillometry (DP) is a simple, non-invasive computerized assessment of pupillary light response which provides data concerning both branches of the autonomous nervous system (ANS). Heart rate variability (HRV) analysis assess cardiac health and the ANS modulation on the heart. In this study, we aimed ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:The diagnostic and staging approach for the mediastinal lymphadenopathies, with or whithout pulmonary lesions endoscopically visible, is based on transbronchial needle aspiration (TBNA) during fiberoptic bronchoscopy and on mediastinoscopy. One important factor impacting on TBNA sensitivity is the rapid on s...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To elucidate whether microRNA-374b could participate in the development of lung cancer (LC) through downregulating PTEN (gene of phosphate and tensin homolog deleted on chromosome ten) expression via activating PI3K/Akt pathway. PATIENTS AND METHODS:Expression levels of microRNA-374b and PTEN in LC tissues a...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201902_17002
更新日期：2019-02-01 00:00:00
abstract：OBJECTIVE:This study aimed to detect the expression of microRNA-378 in OSCC, and further studies its effects on clinicopathology and prognosis of OSCC patients. PATIENTS AND METHODS:Real-Time quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect the expression levels of microRNA-378 in 96 pairs of OSCC t...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201907_18437
更新日期：2019-07-01 00:00:00
abstract：:Mucoepidermoid carcinoma (MEC) of the skin is an extremely rare neoplasm but is common in the major and minor salivary glands accounting of approximately 30% of all malignant tumors arising from these glands. Cutaneous involvement should be carefully assessed to exclude the possibility of metastases from distant sites...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2012-10-01 00:00:00
abstract：OBJECTIVE:The aim of this work was to explore whether lncRNA-MEG3 could serve as a serum biomarker for diagnosing chronic hepatitis B (CHB) and improve the early diagnostic and treatment efficacies. PATIENTS AND METHODS:Serum level of lncRNA-MEG3 in CHB patients and healthy controls was detected by quantitative Real T...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201905_17943
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:To investigate the association between serum uric acid levels and the risk of incident atrial fibrillation in patients after coronary artery bypass graft (cABG) operation. PATIENTS AND METHODS:A total of 174 patients undergoing nonemergency coronary artery bypass graft operation were included in the study. P...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:The ubiquitin-conjugating enzyme E2C (UBE2C) has been known as a crucial factor upregulated in various tumors. The functions of UBE2C is mainly involved in the pathway protein ubiquitination. This study investigates the expression of UBE2C in gastric cancers and its correlation with overall survival rate. MA...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201803_14578
更新日期：2018-03-01 00:00:00
abstract：OBJECTIVE:This study examines the relationship between intracranial pressure (ICP) changes after skull injury repair and neurocognitive function before and after the repair. PATIENTS AND METHODS:Sixty patients undergoing skull injury repair participated in the study. A non-invasive detection analyzer was used to detec...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-03-01 00:00:00
abstract：AIM:γ-oryzanol (OZ) is a rich source of commercially-important bioactive phytochemicals, most of them of interest in nutrition, pharmacy and cosmetics. However, the poor solubility of OZ limited the use. In the paper, ultraviolet-visible (UV-Vis) analysis was conducted to analysis the solubilization of OZ under combine...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:Oral squamous cell carcinoma (OSCC) is still one of the most frequent neck and head malignancies and is one of the most common cancers in the world. The main purpose of this research was to illustrate the functional role of LINC00961 in OSCC and provide novel insight of biomarkers and therapeutic strategies i...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17699
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:Post-tonsillectomy pain is believed to be mediated by noxious stimulation of C-fiber afferents located in the peritonsillary space, and local anesthetic infiltration to this area may decrease pain by blocking the sensory pathways and thus preventing the nociceptive impulses. We aimed to compare the effects of...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2015-04-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to observe the association between N-myc downstream regulated gene 2 (NDRG2)/interleukin-6 (IL-6)/signal transducer and activator of transcription 3 (STAT3) signaling pathway and diabetic retinopathy (DR) in rats. MATERIALS AND METHODS:The model of diabetes was successfully establis...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202004_20806
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVE:To analyze the different influence of body position on wireless high-resolution anorectal manometry parameters and in classification for chronic constipation patients. PATIENTS AND METHODS:Fifty consecutive patients with chronic constipation and 20 healthy volunteers were included in this study, all of whom ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201910_19162
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVE:The development of multidrug resistance (MDR) is a key issue for tumor recurrence and metastasis, leading to treatment failure of gastric cancer (GC). Long non-coding RNA (lncRNA) DANCR has been shown to be highly expressed in GC patients, which accelerates growth and metastasis of GC cells. This study aims t...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17554
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:Laryngeal cancer is a common malignant tumor in the head and neck, which affects swallowing, breathing, and pronunciation function. In recent years, many long non-coding RNAs (lncRNAs) have been shown to be involved in the progression of cancer with the development of gene sequencing, transcriptomics, and bio...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201804_14723
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore the effects of micro ribonucleic acid (miR)-506 and phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) pathway on myocardial ischemia-reperfusion injury (MIRI) in rats. MATERIALS AND METHODS:A total of 90 healthy rats weighing 260-300 g were selected as r...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202012_24193
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:The aim of the study is to explore the potential familial hypercholesterolemia markers by comparing with healthy controls. MATERIAL AND METHODS:We downloaded the gene expression profile GSE13985 from Gene Expression Omnibus database including five patients diagnosed with familial hypercholesterolemia (FH) an...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：OBJECTIVE:Our study aims to demonstrate that the use in the preconceptional period until the 24th week of pregnancy of inositol and folic acid, first of all, preserves the product of conception from neural tube defects (NTDs) and then, thanks to inositol supplementation, it possibly counteracts and prevents the onset o...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:To study the mechanism of lncRNA GAS5 affecting epithelial-mesenchymal transition and invasion of breast cancer cells by regulating miR-216b. PATIENTS AND METHODS:Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expressions of GAS5 and miR-216b in breast cancer and paracancer...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202005_21176
更新日期：2020-05-01 00:00:00
abstract：OBJECTIVE:To investigate the safety and effectiveness of the application of high-volume hemofiltration (HVHF) in children with sepsis combined with acute kidney injury. PATIENTS AND METHODS:A total of 76 child patients were enrolled and randomly divided equally (n=38) into control and the observation groups respective...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：10.26355/eurrev_201802_14298
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:To explore a new approach for treating renal insufficiency with gene therapy by implanting decorin (DCN)-expressing fibroblasts within the renal tissue of rats with renal failure to neutralize TGF-β1 activity. MATERIALS AND METHODS:The 5/6 kidney of the selected male SD rats were removed under aseptic condit...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201804_14843
更新日期：2018-04-01 00:00:00
abstract：BACKGROUND:Lung adenocarcinoma (LAC) is the most common type of lung cancer, accounting for 30-35% of all cases. AIM:In this study we aim to predict potential genes and confirm pathways which are associated with LAC. MATERIALS AND METHODS:By using the meta-analysis method, GSE10072 and GSE 2514 datasets were merged t...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,meta分析
doi：
更新日期：2012-09-01 00:00:00
abstract：:The purpose of this study was to identify new indexes of body composition that characterize the normal weight obese (NWO) women. We measured body composition by dual energy x-ray absorptiometry (DXA) and resting metabolic rate (RMR) by indirect calorimetry in a cohort of seventy-five healthy Italian women, subdivided ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Cardiovascular diseases remain one of the major health problems worldwide. The worldwide research against cardio-vascular diseases as well as genome wide association studies were successful in indentifying the loci associated with these prominent life threatening diseases but still a substantial amount of casualty rem...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND:To this date, only a small number of studies have described the long-term use of BTX in Hemifacial spasm. AIM:To assess the prognosis and long-term effectiveness of Botulinum toxin A (BTXA) in the treatment of unilateral spasms of the eyelid. PATIENTS AND METHODS:From September 1998 to January 2006, 245 co...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:To illustrate the role of interleukin 6 (IL-6) in the progression of non-small cell lung cancer (NSCLC) via activating STAT1. PATIENTS AND METHODS:The level of IL-6 mRNA in 48 paired NSCLC tissues and matched normal ones was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Kaplan-Mei...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202004_20837
更新日期：2020-04-01 00:00:00
abstract：OBJECTIVE:To uncover the role of UPF1 in alleviating the progression of glioma via targeting long non-coding RNA (lncRNA) CYTOR and underlying mechanism. PATIENTS AND METHODS:A total of 30 glioma tissues surgically resected from glioma patients and 30 brain tissues were collected from brain trauma patients undergoing ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201911_19567
更新日期：2019-11-01 00:00:00
abstract：OBJECTIVE:To investigate the larvicidal and ovicidal activities of acetone, benzene, hexane and methanol leaf extracts of Basella rubra and Cleome viscosa against dengue vector, Aedes aegypti. MATERIALS AND METHODS:Twenty five early third instar larvae of Aedes aegypti were exposed to various concentrations and were a...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-05-01 00:00:00
